The over-voltage effect on a LiFePO4-based cell is discussed in this article. A full overview of the infrastructure required to accomplish a controlled over-voltage of an electric cell is provided. This article examines the waveform of cell over-voltage, changes in electrical parameters, cell temperature, and cell size change in detail. The influence of over-voltage on the parameters of the internal equivalent replacement electrical scheme is the major focus of the article. The detailed parameter change of elements R and C of the equivalent replacement electrical scheme is depicted graphically in relation to the quantity of energy contained in the tested cell (current SOC).