Life Processes Research Articles

Links Between Daily Life and Laboratory Emotion Regulation Processes: The Role of Age and Cognitive Status.

This study investigates how daily use of emotion regulation (ER) strategies predicts ER processes in the laboratory among young adults and cognitively diverse older adults. Young adults (aged 21-34, n=66), cognitively normal older adults (CN; aged 70-83, n=87), and older adults with researcher-defined mild cognitive impairment (MCI; aged 70-84; n=58) completed an experience sampling procedure (7x/day for 9 days) reporting their distraction and reappraisal use in daily life. In a laboratory task inducing high-arousal negative emotion, they reported their (a) distraction and reappraisal use when instructed to reduce negative emotion and (b) ER success and perceptions when randomly assigned to regulate using distraction or reappraisal. Among CN older adults, higher frequency of using a strategy in daily life predicted greater success deploying the strategy when instructed to do so but was unrelated to spontaneous strategy use in the laboratory. In contrast, among older adults with researcher-defined MCI, greater daily life strategy use predicted greater laboratory use, but not greater success. Daily strategy use in younger adults was unrelated to strategy use and success in the laboratory. Older adults with researcher-defined MCI experienced ER as more demanding but did not differ from non-impaired individuals in terms of perceived ER effort. Cognitively normal older adults may be better able to leverage their ER experience in novel contexts than younger adults. Older adults with MCI may be motivated to manage their emotions but experience more ER difficulty, perhaps in part due to reliance on default strategies.

Modes of Presentation, Diagnostic Difficulties and Pattern of Management of Carcinoma of Stomach: Study of 100 Cases Admitted in SBMCH, Barisal

Background: Aging is inevitable process of life. In Bangladesh, the number of older people is growing rapidly due to falling fertility and increased expectancy of life. Objective: We aimed to determine the prevalence of self-reported morbidity, to describe the morbidity pattern, health-seeking behavior, and socio-demographic profile, among the rural elderly people of the Chattogram district of Bangladesh. Methods: A descriptive cross-sectional study was conducted at 16 villages under 8 unions of the Mirsarai upazilla of the Chattogram district conveniently. Households were selected by systematic random sampling. Data were collected through face-to-face interviews from 200 participants. We used a semi-structured questionnaire. It was divided into two parts: questions related to socio demographic characteristics, questions related to morbidity pattern and health seeking behavior. Descriptive statistics and chi-square test were done. Data was cleaned and prepared with Microsoft Excel and analysis was performed using SPSS, version 23. Results: Mean age of the respondents was 66.5 years. Only (10.5%) had no morbidity and more than two-thirds (70%) of the respondents had multimorbidity. Most common morbidities identified among them were osteoarthritis (66.5%) and hypertension (51%) followed by chronic obstructive pulmonary disease (29.5%), ischemic heart disease (25%), Diabetes mellitus (21%), etc. Among them, (80%) seek treatment for their health problems. The majority (27%) preferred going to the government hospital and one-fourth (25%) preferred a private doctor. Among older people not seeking treatment for their medical condition, a majority (47.5%) said they didn’t feel the need to go to a doctor and (35%) said that they had financial problems. Conclusion: A high prevalence of morbidity among the elderly population is found, suggesting that geriatric care should become an integral part of primary health care. Regular screening and health education need to be ........

Using Machine Learning for Chronic Disease Diagnosis and Prediction

Machine learning had a strong effect on day-to-day life processes, and in the future, it's going to continue influencing this field. A key where machine learning illustrates promise in healthcare, especially in diagnosing and predicting chronic diseases, conditions that last for at least a year. This paper examines various methods through which machine learning is used in healthcare, consider how these models might be improved to perform specialized tasks, like detecting cancer from individual cells, as they are used to analyse medical images. The paper also explores the inner workings of machine learning and enlightens on some of its current limitations. The article addresses concerns such as model bias and the significant expenses associated with implementing machine learning solutions

CD4+ T-Cell Senescence in Neurodegenerative Disease: Pathogenesis and Potential Therapeutic Targets.

With the increasing proportion of the aging population, neurodegenerative diseases have become one of the major health issues in society. Neurodegenerative diseases (NDs), including multiple sclerosis (MS), Alzheimer's disease (AD), Parkinson's disease (PD), and amyotrophic lateral sclerosis (ALS), are characterized by progressive neurodegeneration associated with aging, leading to a gradual decline in cognitive, emotional, and motor functions in patients. The process of aging is a normal physiological process in human life and is accompanied by the aging of the immune system, which is known as immunosenescence. T-cells are an important part of the immune system, and their senescence is the main feature of immunosenescence. The appearance of senescent T-cells has been shown to potentially lead to chronic inflammation and tissue damage, with some studies indicating a direct link between T-cell senescence, inflammation, and neuronal damage. The role of these subsets with different functions in NDs is still under debate. A growing body of evidence suggests that in people with a ND, there is a prevalence of CD4+ T-cell subsets exhibiting characteristics that are linked to senescence. This underscores the significance of CD4+ T-cells in NDs. In this review, we summarize the classification and function of CD4+ T-cell subpopulations, the characteristics of CD4+ T-cell senescence, the potential roles of these cells in animal models and human studies of NDs, and therapeutic strategies targeting CD4+ T-cell senescence.

Morbidity Pattern and Health Seeking Behaviour among the Geriatric People of Rural Areas of Chattogram District in Bangladesh

Background: Aging is inevitable process of life. In Bangladesh, the number of older people is growing rapidly due to falling fertility and increased expectancy of life. Objective: We aimed to determine the prevalence of self-reported morbidity, to describe the morbidity pattern, health-seeking behavior, and socio-demographic profile, among the rural elderly people of the Chattogram district of Bangladesh. Methods: A descriptive cross-sectional study was conducted at 16 villages under 8 unions of the Mirsarai upazilla of the Chattogram district conveniently. Households were selected by systematic random sampling. Data were collected through face-to-face interviews from 200 participants. We used a semi-structured questionnaire. It was divided into two parts: questions related to socio demographic characteristics, questions related to morbidity pattern and health seeking behavior. Descriptive statistics and chi-square test were done. Data was cleaned and prepared with Microsoft Excel and analysis was performed using SPSS, version 23. Results: Mean age of the respondents was 66.5 years. Only (10.5%) had no morbidity and more than two-thirds (70%) of the respondents had multimorbidity. Most common morbidities identified among them were osteoarthritis (66.5%) and hypertension (51%) followed by chronic obstructive pulmonary disease (29.5%), ischemic heart disease (25%), Diabetes mellitus (21%), etc. Among them, (80%) seek treatment for their health problems. The majority (27%) preferred going to the government hospital and one-fourth (25%) preferred a private doctor. Among older people not seeking treatment for their medical condition, a majority (47.5%) said they didn’t feel the need to go to a doctor and (35%) said that they had financial problems. Conclusion: A high prevalence of morbidity among the elderly population is found, suggesting that geriatric care should become an integral part of primary health care. Regular screening and health education need to be.........

Empirical Research of Professional Creativity of the Head of Educational Institution

The aim of our research is to show the concepts of “professional creativity” and “creativity in the process of professional activity”; to organize the experimental research and to analyze its results; to describe the processes cause a creative reinterpretation of stereotypes – meaning-making – and changes in the entire life process – life-making; to show the results of empirical researches of professional creativity of the head of educational institutions. Methods of the research. The following theoretical methods of the research were used to solve the tasks formulated in the article: a categorical method, structural and functional methods, the methods of the analysis, systematization, modeling and generalization. The experimental method was the method of organizing empirical research. The results of the research. We proved, that if there was no personal meaning in the process of professional activity, for the sake of which professional activity would be carried out, and only the objective orientation of this activity was expressed. Then such activity would not take the form of creative activity, it would not reflect the actual spiritual search on oneself that is meaningful for a person. The internal objective determinants, the objective orientation of the process of professional activity allows the manager to find his/her own personal meaning, and only when the manager clearly understands why he/she will carry out creative activity, its goals and values will be determined. Only in the case when the process of professional creativity acquires a personally significant meaning for the manager, only then this activity can be considered in a creative aspect, in the paradigm of the manager’s acquisition of personal meanings and the formation of his/her creative individuality. Thus, the professional creativity of a manager is understood by us as a conscious, purposeful, active process of the unique activity of its kind, aimed at finding a new, independent, innovative and original way of managing the activities of subordinates with the aim of highly effective creative resolution of managerial tasks and problems. Conclusions. The results of professional creativity are: a new understanding of the subject of activity – the creation of new ideas, programs, concepts, projects, paradigms, aimed at obtaining fundamentally new results, which make it possible to involve a wider social community in creative cooperation; the emergence of new ways of carrying out professional activities, hitherto unknown technologies, activity algorithms, etc. Professional creativity of the manager is accompanied by constant personal development – the need for new, original, non-standard and unique ideas.

Identifikasi Permasalahan Yang Dihadapi Guru PJOK DALAM Pelaksanaan Pembelajaran di SMAK ST. Thomas Aquinas Ruteng

Education is the most important thing in human life, this means that every Indonesian person deserves it and is expected to always develop in it, education will never end, education in general has the meaning of a life process in developing each individual to be able to live and live life. The purpose of this study is to find out what are the obstacles faced by physical education teachers at SMAK St. Thomas Aquinas Ruteng and to find out what factors influence the problems or obstacles faced by physical education teachers at SMAK St. Thomas Aquinas Ruteng. This research is a research that uses a qualitative approach. The research subjects in this study were school principals, physical education teachers (2 teachers) and also students (3 children). The main method in this study uses the interview method used to obtain direct information about the problems faced in physical education learning. The results showed that there are 4 factors that affect physical education learning, namely teacher factors, student factors, curriculum factors and environmental factors. From the results of the study, it was concluded that, 1.) Physical education learning at SMAK St. Thomas Aquinas Ruteng has adjusted the stages that must be carried out by the physical education teacher, namely curriculum, opening lessons, learning preparation, learning implementation, facilities and infrastructure, time allocation. 2.) The most prominent and problematic factor in PE learning at SMAK St. Thomas Aquinas Ruteng is the lack of enthusiasm from students to follow PE subjects because the facilities and infrastructure are not adequate enough for the implementation of the learning process.

Phraseological verbs in Heydar Aliyev's speech

In this article which studies Heydar Aliyev's speech from the point of view of linguistics, 20 phraseological verbs are examined, and the reasons why the great leader used them are explained. There are such phraseologiycal verbs in this post that they are political-diplomatic contents — such as “to put a veto”, “to adress a speech”, “o deliver a speech”, “to take responsibility”, “to raise a question”. There is such that it is refugee content like “to fall into trouble”. There are those who are literary-poetic-art - such as “to go into ecstasy”, “to give a stimulation”, “to put pen to paper”. The rest are active verbs in the daily life process - such as “to draw a plan” and so on.

Single-Molecule Electrical Profiling of Peptides and Proteins.

In recent decades, there has been a significant increase in the application of single-molecule electrical analysis platforms in studying proteins and peptides. These advanced analysis methods have the potential for deep investigation of enzymatic working mechanisms and accurate monitoring of dynamic changes in protein configurations, which are often challenging to achieve in ensemble measurements. In this work, the prominent research progress in peptide and protein-related studies are surveyed using electronic devices with single-molecule/single-event sensitivity, including single-molecule junctions, single-molecule field-effect transistors, and nanopores. In particular, the successful commercial application of nanopores in DNA sequencing has made it one of the most promising techniques in protein sequencing at the single-molecule level. From single peptides to protein complexes, the correlation between their electrical characteristics, structures, and biological functions is gradually being established. This enables to distinguish different molecular configurations of these biomacromolecules through real-time electrical monitoring of their life activities, significantly improving the understanding of the mechanisms underlying various life processes.

Cellular ATP redistribution achieved by deleting Tgparp improves lignocellulose utilization of Trichoderma under heat stress.

Thermotolerance is widely acknowledged as a pivotal factor for fungal survival across diverse habitats. Heat stress induces a cascade of disruptions in various life processes, especially in the acquisition of carbon sources, while the mechanisms by which filamentous fungi adapt to heat stress and maintain carbon sources are still not fully understood. Using Trichoderma guizhouense, a representative beneficial microorganism for plants, we discover that heat stress severely inhibits the lignocellulases secretion, affecting carbon source utilization efficiency. Proteomic results at different temperatures suggest that proteins involved in the poly ADP-ribosylation pathway (TgPARP and TgADPRase) may play pivotal roles in thermal adaptation and lignocellulose utilization. TgPARP is induced by heat stress, while the deletion of Tgparp significantly improves the lignocellulose utilization capacity and lignocellulases secretion in T. guizhouense. Simultaneously, the absence of Tgparp prevents the excessive depletion of ATP and NAD+, enhances the protective role of mitochondrial membrane potential (MMP), and elevates the expression levels of the unfolded protein response (UPR)-related regulatory factor Tgire. Further investigations reveal that a stable MMP can establish energy homeostasis, allocating more ATP within the endoplasmic reticulum (ER) to reduce protein accumulation in the ER, thereby enhancing the lignocellulases secretion in T. guizhouense under heat stress. Overall, these findings underscored the significance of Tgparp as pivotal regulators in lignocellulose utilization under heat stress and provided further insights into the molecular mechanism of filamentous fungi in utilizing lignocellulose.

PCR Independent Strategy-Based Biosensors for RNA Detection

RNA is an important information and functional molecule. It can respond to the regulation of life processes and is also a key molecule in gene expression and regulation. Therefore, RNA detection technology has been widely used in many fields, especially in disease diagnosis, medical research, genetic engineering and other fields. However, the current RT-qPCR for RNA detection is complex, costly and requires the support of professional technicians, resulting in it not having great potential for rapid application in the field. PCR-free techniques are the most attractive alternative. They are a low-cost, simple operation method and do not require the support of large instruments, providing a new concept for the development of new RNA detection methods. This article reviews current PCR-free methods, overviews reported RNA biosensors based on electrochemistry, SPR, microfluidics, nanomaterials and CRISPR, and discusses their challenges and future research prospects in RNA detection.

Assessment of the impact of micro fertilizers on winter wheat and winter barley crops under the Sevan basin conditions

Background: Microelements are crucial for numerous physiological and biochemical processes in plant life. They constitute parts of various vitamins and enzymes, stimulate respiration, and accelerate oxidation and regeneration reactions, positively impacting protein metabolism in plant organisms. Objective: Considering the significant role of microelements in living organisms, our aim is to investigate the effectiveness of applying microfertilizers and biohumus on the growth, development, and quality of winter wheat and winter barley. We conducted field experiments and laboratory research to analyze the impact of trace element content in the soil at our experimental sites. This research holds particular significance amidst the ongoing global climate changes, emphasizing the urgency and relevance of our study․ Methods: Field experiments, both in winter wheat and winter barley, were set up with three replications, with the same fertilization scheme. The size of each plot was 30 m2, and the size of the experimental field in each crop area was 630 m2 consisting of the following types: 1. Control (non-fertilized); 2. Background: biohummus t/ha; 3. Background+Mn (MnSO4 4H2O); 4. Background +Cu (CuSO4. 5H2O); 5. Background+B (Na2 B407.10 H2O); 6. Background + Mo (HH4)2MoO4; 7. Background +Zn (ZnSO45H2O): Microfertilizers were used for pre-sowing seed treatment. Results: The three-year results of the studies revealed that the amount of harvest and the quality indicators of these crops increased from the application of molybdenum, manganese, and copper in the sowing of autumn wheat and autumn barley on the background of biohumus. The increase of the autumn wheat crop under the influence of these elements was 3.3-6.4 c/ha (6.9-13.3%), and the increase of the autumn barley grain crop compared to the biohumus background was 2.6-5.4 c/ha. ha, or 6.5-13.4%. A certain increase in the nitrogen content of wheat and barley grains can be seen as an indicator of a protein problem necessary for life. According to research, it was found that the nitrogen content in the grains of spikelets is high in the versions that received molybdenum, manganese, and copper. Specifically, for wheat, the nitrogen content ranges from 2.64-2.68% for Mo, 2.53-2.57% for Cu, and for barley grains, it ranges from 2.16-2.24% for Mo, 2.08-2.10% for Cu, and 2.02-2.07% for Mn. Simultaneously with the studies, it was proved that the crop yield and quality indicators did not undergo significant changes from the application of Zn and B, which is explained by the higher content of the total and mobile forms of these elements in the soil. Conclusion: In the practice of fertilizing grain crops such as wheat and barley, it's essential to judiciously use specific micro fertilizers like molybdenum ammonium (NH4MoO4), manganese sulfate (MnSO4 · 4H2O), or copper sulfate (CuSO4 · 5H2O). These should be applied alongside organic fertilizers to ensure that the crops receive a comprehensive supply of both microelements and macroelements. This approach not only guarantees the production of high-quality crops but also plays a pivotal role in managing the protein content within them, which is crucial for nutritional value and overall crop health. Keywords: microelements, winter wheat, winter barley, growth and development, yield quantity and quality

“Rich arginine and strong positive charge” antimicrobial protein protamine: From its action on cell membranes to inhibition of bacterial vital functions

Protamine, an antimicrobial protein derived from salmon sperm with a molecular weight of approximately 5 kDa, is composed of 60–70 % arginine and is a highly charged protein. Here, we investigated the mechanism of antimicrobial action of protamine against Cutibacterium acnes (C. acnes) focusing on its rich arginine content and strong positive charge. Especially, we focused on the attribution of dual mechanisms of antimicrobial protein, including membrane disruption or interaction with intracellular components. We first determined the dose-dependent antibacterial activity of protamine against C. acnes. In order to explore the interaction between bacterial membrane and protamine, we analyzed cell morphology, zeta potential, membrane permeability, and the composition of membrane fatty acid. In addition, the localization of protamine in bacteria was observed using fluorescent-labeled protamine. For investigation of the intracellular targets of protamine, bacterial translation was examined using a cell-free translation system. Based on our results, the mechanism of the antimicrobial action of protamine against C. acnes is as follows: 1) electrostatic interactions with the bacterial cell membrane; 2) self-internalization into the bacterial cell by changing the composition of the bacterial membrane; and 3) inhibition of bacterial growth by blocking translation inside the bacteria. However, owing to its strong electric charge, protamine can also interact with DNA, RNA, and other proteins inside the bacteria, and may inhibit various bacterial life processes beyond the translation process.

Stretchable Photonic Crystal-Assisted Glycoprotein Identification for Ovarian Cancer Diagnosis.

Photonic crystals with specific wavelengths can realize surface-enhanced excitation and emission intensities of fluorophores and enhance the fluorescence signals of fluorescent molecules. Herein, stretchable photonic crystals with good mechanochromic properties provide continuously adjustable forbidden wavelengths by stretching to change the lattice spacing, with reflectance peaks blue-shifted up to 110 nm to match indicators of different wavelengths and produce differentiated optical enhancement effects. Glycoproteins are significantly identified as clinical markers. However, the wide participation of glycoproteins in various life processes poses enormous complexity and critical challenges for rapid, facile, high-throughput, and accurate clinical analysis or health assessment. In this work, we proposed a stretchable photonic crystal-assisted glycoprotein identification approach for early ovarian cancer diagnosis. Stretchable photonic crystals can provide rich optical information to efficiently identify glycoproteins in complex matrices. A double-indicator fluorescence sensor was designed to respond to the protein trunk and oligosaccharide segment of glycoproteins separately for improved recognition accuracy. Seven typical glycoproteins could be discriminated from proteins, saccharides, or mixture interferents. Clinical ovarian cancer samples for early, intermediate, and advanced ovarian cancer and healthy subjects were verified with 100% accuracy. This strategy of stretchable photonic crystal-assisted glycoprotein identification provides an effective method for accurate, rapid ovarian cancer diagnosis and timely clinical treatment.

BmATAD3A mediates mitochondrial ribosomal protein expression to maintain the mitochondrial energy metabolism of the silkworm, Bombyx mori.

ATAD3A is a mitochondrial membrane protein belonging to the ATPase family that contains the AAA+ domain. It is widely involved in mitochondrial metabolism, protein transport, cell growth, development and other important life processes. It has previously been reported that the deletion of ATAD3A causes growth and development defects in humans, mice and Caenorhabditis elegans. To delve into the mechanism underlying ATAD3A defects and their impact on development, we constructed a Bombyx mori ATAD3A (BmATAD3A) defect model in silkworm larvae. We aim to offer a reference for understanding ATAD3A genetic defects and elucidating the molecular regulatory mechanisms. The results showed that knockout of the BmATAD3A gene significantly affected the weight, survival rate, ATPase production and mitochondrial metabolism of individuals after 24h of incubation. Combined metabolomics and transcriptomics analysis further demonstrated that BmATAD3A knockout inhibits amino acid biosynthesis through the regulation of mitochondrial ribosomal protein expression. Simultaneously, our findings indicate that BmATAD3A knockout impeded mitochondrial activity and ATPase synthesis and suppressed the mitochondrial oxidative phosphorylation pathway through B.mori mitochondrial ribosomal protein L11 (BmmRpL11). These results provide novel insights into the molecular mechanisms involved in the inhibition of development caused by ATAD3A deficiency, offering a potential direction for targeted therapy in diseases associated with abnormal ATAD3A expression.

Synthetic Peptides with Genetic‐Codon‐Tailored Affinity for Assembling Tetraspanin CD81 at Cell Interfaces and Inhibiting Cancer Metastasis

AbstractProbing biomolecular interactions at cellular interfaces is crucial for understanding and interfering with life processes. Although affinity binders with site specificity for membrane proteins are unparalleled molecular tools, a high demand remains for novel multi‐functional ligands. In this study, a synthetic peptide (APQQ) with tight and specific binding to the untargeted extracellular loop of CD81 evolved from a genetically encoded peptide pool. With tailored affinity, APQQ flexibly accesses, site‐specifically binds, and forms a complex with CD81, enabling in‐situ tracking of the dynamics and activity of this protein in living cells, which has rarely been explored because of the lack of ligands. Furthermore, APQQ triggers the relocalization of CD81 from diffuse to densely clustered at cell junctions and modulates the interplay of membrane proteins at cellular interfaces. Motivated by these, efficient suppression of cancer cell migration, and inhibition of breast cancer metastasis were achieved in vivo.

Synthetic Peptides with Genetic-Codon-Tailored Affinity for Assembling Tetraspanin CD81 at Cell Interfaces and Inhibiting Cancer Metastasis.

Probing biomolecular interactions at cellular interfaces is crucial for understanding and interfering with life processes. Although affinity binders with site specificity for membrane proteins are unparalleled molecular tools, a high demand remains for novel multi-functional ligands. In this study, a synthetic peptide (APQQ) with tight and specific binding to the untargeted extracellular loop of CD81 evolved from a genetically encoded peptide pool. With tailored affinity, APQQ flexibly accesses, site-specifically binds, and forms a complex with CD81, enabling in-situ tracking of the dynamics and activity of this protein in living cells, which has rarely been explored because of the lack of ligands. Furthermore, APQQ triggers the relocalization of CD81 from diffuse to densely clustered at cell junctions and modulates the interplay of membrane proteins at cellular interfaces. Motivated by these, efficient suppression of cancer cell migration, and inhibition of breast cancer metastasis were achieved in vivo.

Cation Triggered Self-Assembly of α-Lactalbumin Nanotubes.

Metal ions play a dual role in biological systems. Although they actively participate in vital life processes, they may contribute to protein aggregation and misfolding and thus contribute to development of diseases and other pathologies. In nanofabrication, metal ions mediate the formation of nanostructures with diverse properties. Here, we investigated the self-assembly of α-lactalbumin into nanotubes induced by coordination with metal ions, screened among the series Mn2+, Co2+, Ni2+, Zn2+, Cd2+, and Au3+. Our results revealed that the affinity of metal ions toward hydrolyzed α-lactalbumin peptides not only impacts the kinetics of nanotube formation but also influences their length and rigidity. These findings expand our understanding of supramolecular assembly processes in protein-based materials and pave the way for designing novel materials such as metallogels in biochip and biosensor applications.

Synthetic Peptides: Promising Modalities for the Targeting of Disease-Related Nucleic Acids.

DNA and RNA play pivotal roles in life processes by storing and transferring genetic information, modulating gene expression, and contributing to essential cellular machinery such as ribosomes. Dysregulation and mutations in nucleic acid-related processes are implicated in numerous diseases. Despite the critical impact on health of nucleic acid mutations or dysregulation, therapeutic compounds addressing these biomolecules remain limited. Peptides have emerged as a promising class of molecules for biomedical research, offering potential solutions for challenging drug targets. This review focuses on the use of synthetic peptides to target disease-related nucleic acids. We discuss examples of peptides targeting double-stranded DNA, including the clinical candidate Omomyc, and compounds designed for regulatory G-quadruplexes. Further, we provide insights into both library-based screenings and the rational design of peptides to target regulatory human RNA scaffolds and viral RNAs, emphasizing the potential of peptides in addressing nucleic acid-related diseases.

Synthetic Peptides: Promising Modalities for the Targeting of Disease‐Related Nucleic Acids

AbstractDNA and RNA play pivotal roles in life processes by storing and transferring genetic information, modulating gene expression, and contributing to essential cellular machinery such as ribosomes. Dysregulation and mutations in nucleic acid‐related processes are implicated in numerous diseases. Despite the critical impact on health of nucleic acid mutations or dysregulation, therapeutic compounds addressing these biomolecules remain limited. Peptides have emerged as a promising class of molecules for biomedical research, offering potential solutions for challenging drug targets. This review focuses on the use of synthetic peptides to target disease‐related nucleic acids. We discuss examples of peptides targeting double‐stranded DNA, including the clinical candidate Omomyc, and compounds designed for regulatory G‐quadruplexes. Further, we provide insights into both library‐based screenings and the rational design of peptides to target regulatory human RNA scaffolds and viral RNAs, emphasizing the potential of peptides in addressing nucleic acid‐related diseases.