Life Cycle Transitions Research Articles

Ethical and Intrapsychic Issues in Ending a Practice

Ethical and Intrapsychic Issues in Ending a Practice

Women’s Leadership and Transnational Currents: The Adhiparasakthi Community in Toronto, Canada

This article analyzes the ways in which the Adhiparasakthi community in Toronto, Canada is linked to the larger transnational Adhiparasakthi organization in Melmaruvathur, India and illustrates how women’s ritual authority, congregational pūjā, and “inclusivity” ethics have shaped the identity of this diaspora community locally. As women serve in positions of ritual authority and leadership within the Adhiparasakthi Temple Society of Canada, they are in a position to effect change. The article further addresses some of the particular concerns within the community, such as those regarding rituals, lifecycle transitions, and fragmented support networks, and shows how some members of the community have found resolutions through the auspices of the mandram (temple). As women’s ritual lives and interpretations of the divine feminine converge with sevā (humanitarian work) in the Toronto context, their leadership and engagement has emerged as a current of support in the mandram community.

Impact of household transitions on domestic energy consumption and its applicability to urban energy planning

The household sector consumes roughly 30% of Earth’s energy resources and emits approximately 17% of its carbon dioxide. As such, developing appropriate policies to reduce the CO2 emissions, which are associated with the world’s rapidly growing urban population, is a high priority. This, in turn, will enable the creation of cities that respect the natural environment and the well-being of future generations. However, most of the existing expertise focuses on enhancing the thermal quality of buildings through building physics while few studies address the social and behavioral aspects. In fact, focusing on these aspects should be more prominent, as they cause between 4% and 30% of variation in domestic energy consumption. Premised on that, the aim of this study was to investigate the effect in the context of the UK of household transitions on household energy consumption patterns. To achieve this, we applied statistical procedures (e.g., logistic regression) to official panel survey data comprising more than 5500 households in the UK tracked annually over the course of 18 years. This helped in predicting future transition patterns for different household types for the next 10 to 15 years. Furthermore, it enabled us to study the relationship between the predicted patterns and the household energy usage for both gas and electricity. The findings indicate that the life cycle transitions of a household significantly influence its domestic energy usage. However, this effect is mostly positive in direction and weak in magnitude. Finally, we present our developed urban energy model “EvoEnergy” to demonstrate the importance of incorporating such a concept in energy forecasting for effective sustainable energy decision-making.

Ontogenetic Changes in the Bacterial Symbiont Community of the Tropical Demosponge Amphimedon queenslandica: Metamorphosis Is a New Beginning

Vertical transmission of bacterial symbionts, which is known in many species of sponge (Porifera), is expected to promote strong fidelity between the partners. Combining 16S rRNA gene amplicon sequencing and electron microscopy, we have assayed the relative abundance of vertically-inherited bacterial symbionts in several stages of the life cycle of Amphimedon queenslandica, a tropical coral reef sponge. We reveal that adult A. queenslandica house a low diversity microbiome dominated by just three proteobacterial OTUs, with a single gammaprotebacterium clearly dominant through much of the life cycle. This ontogenetic perspective has revealed that, although vertical transmission occurs very early in development, the inherited symbionts do not maintain proportional dominance of the bacterial community at every developmental stage. A reproductive bottleneck in the A. queenslandica life cycle is larval settlement, when a free-swimming pelagic larva settles out of the water column onto the benthos and completes metamorphoses into the sessile body plan within just 3 to 4 days. During this dramatic life cycle transition, an influx of environmentally-derived bacteria leads to a major reorganization of the microbiome, potentially challenging the fidelity and persistence of the vertically-inherited symbiotic relationships. However, dominance of the primary, vertically-inherited symbionts is restored in adult sponges. The mechanisms underlying ontogenetic changes in the bacterial community are unknown, including how the dominance of the primary symbionts is restored in the adult sponge – does the host or symbiont regulate this process? Using high-resolution transcriptional profiling in multiple stages of the A. queenslandica life cycle combined with this natural perturbation of the microbiome immediately following larval settlement, we are beginning to identify candidate host genes associated with animal-bacterial crosstalk. Among the sponge host genes upregulated during the times of active microbiome assembly, there is an enrichment of genes potentially involved in innate immunity, including scavenger receptors, and of genes containing eukaryote-like domains, which have elsewhere been implicated in host-symbiont interactions. Intriguingly, we also see an enrichment of sponge genes arising from ancient horizontal transfer events from bacteria, which raises the possibility that host-bacterial associations in the evolutionary past may help to regulate host-bacterial associations in the ecological present.

High-Throughput Proteomics Reveals the Unicellular Roots of Animal Phosphosignaling and Cell Differentiation

Cell-specific regulation of protein levels and activity is essential for the distribution of functions among multiple cell types in animals. The finding that many genes involved in these regulatory processes have a premetazoan origin raises the intriguing possibility that the mechanisms required for spatially regulated cell differentiation evolved prior to the appearance of animals. Here, we use high-throughput proteomics in Capsaspora owczarzaki, a close unicellular relative of animals, to characterize the dynamic proteome and phosphoproteome profiles of three temporally distinct cell types in this premetazoan species. Weshow that life-cycle transitions are linked to extensive proteome and phosphoproteome remodeling and that they affect key genes involved in animal multicellularity, such as transcription factors and tyrosine kinases. The observation of shared features between Capsaspora and metazoans indicates that elaborate and conserved phosphosignaling and proteome regulation supported temporal cell-type differentiation in the unicellular ancestor of animals.

Enabling technologies, lifecycle transitions, and industrial systems in technology foresight: Insights from advanced materials FTA

This paper investigates opportunities to enhance Future-oriented Technology Analysis (FTA) of emerging technologies within innovation systems. We address key challenges faced by policymakers developing innovation strategies for emerging technologies. In particular, we explore ways that FTA might be structured to investigate the complex innovation system journeys of novel technologies as they are developed, diffused, and deployed. In doing this, we draw on concepts from technology and operations management and related literatures to more carefully characterise the: (1) ‘technical infrastrucutre’ required to develop emerging technologies; (2) key technology transitions involved in diffusion; and (3) complex industrial value networks into which they may eventually get deployed.We investigate the extent to which these categories are already used within national technology foresight exercises. In particular, we review over 240 international FTA-related policy, strategy, and analysis documents for ‘advanced materials’. We find that – although generally used inconsistently and unsystematically within FTA – these categories repeatedly emerge as important elements in many policies, strategies, and underpinning foresight exercises. We conclude by arguing that these categories should be carefully considered in initial FTA design. And that, by doing so, FTA exercises may better reveal potential ‘innovation system failures’ and help policymakers coordinate policy actions in response.

Contributions of dynamic environmental signals during life-cycle transitions to early life-history traits in lodgepole pine (<i>Pinus contorta</i> Dougl.)

Abstract. Environmental signals are important triggers in the life-cycle transitions and play a crucial role in the life-history evolution. Yet very little is known about the leading ecological factors contributing to the variations of life-history traits in perennial plants. This paper explores both the causes and consequences for the evolution of life-history traits (i.e., seed dormancy and size) in lodgepole pine (Pinus contorta Dougl.) across British Columbia (B.C.), Canada. We selected 83 logepole pine populations covering 22 ecosystem zones of B.C. and through their geographic coordinate, 197 climatic variables were generated accordingly for the reference (1961–1990) and future (2041–2070) periods. We found that dynamic climatic variables rather than constant geographic variables are the true environmental driving forces in seed dormancy and size variations and thus provide reliable predictors in response to global climate change. Evapotranspiration and precipitation in the plant-to-seed chronology are the most critical climate variables for seed dormancy and size variations, respectively. Hence, we predicted that levels of seed dormancy in lodgepole pine would increase across large tracts of B.C. in 2050s. Winter-chilling is able to increase the magnitude of life-history plasticity and lower the bet-hedge strategy in the seed-to-plant transition; however, winter-chilling is likely to be insufficient in the north of 49° N in 2050s, which may delay germination while unfavorable conditions during dry summers may result in adverse consequences in the survival of seedlings owing to extended germination span. These findings provide useful information to studies related to assessments of seed transfer and tree adaptation.

The Dynamic Regulatory Genome of Capsaspora and the Origin of Animal Multicellularity

SummaryThe unicellular ancestor of animals had a complex repertoire of genes linked to multicellular processes. This suggests that changes in the regulatory genome, rather than in gene innovation, were key to the origin of animals. Here, we carry out multiple functional genomic assays in Capsaspora owczarzaki, the unicellular relative of animals with the largest known gene repertoire for transcriptional regulation. We show that changing chromatin states, differential lincRNA expression, and dynamic cis-regulatory sites are associated with life cycle transitions in Capsaspora. Moreover, we demonstrate conservation of animal developmental transcription-factor networks and extensive network interconnection in this premetazoan organism. In contrast, however, Capsaspora lacks animal promoter types, and its regulatory sites are small, proximal, and lack signatures of animal enhancers. Overall, our results indicate that the emergence of animal multicellularity was linked to a major shift in genome cis-regulatory complexity, most notably the appearance of distal enhancer regulation.

Calcium Signaling in Oomycetes: An Evolutionary Perspective.

Oomycetes are a family of eukaryotic microbes that superficially resemble fungi, but which are phylogenetically distinct from them. These organisms cause major global economic losses to agriculture and fisheries, with representative pathogens being Phytophthora infestans, the cause of late potato blight and Saprolegnia diclina, the instigator of “cotton molds” in fish. As in all eukaryotes, cytoplasmic Ca2+ is a key second messenger in oomycetes, regulating life-cycle transitions, controlling motility and chemotaxis and, in excess, leading to cell-death. Despite this, little is known about the molecular mechanisms regulating cytoplasmic Ca2+ concentrations in these organisms. Consequently, this review analyzed the presence of candidate calcium channels encoded within the nine oomycete genomes that are currently available. This revealed key differences between oomycetes and other eukaryotes, in particular the expansion and loss of different channel families, and the presence of a phylum-specific group of proteins, termed the polycystic kidney disease tandem ryanodine receptor domain (PKDRR) channels.

Demography and population growth rate of the tree Prosopis flexuosa with contrasting grazing regimes in the Central Monte Desert

One of the most important current challenges for ecologists is to evaluate how human-induced changes in ecosystems would impact viability of populations. Demographic response to anthropogenic impact could help us to understand how to manage those impacts. Using demographic techniques and population projection models, here we assess if demography and population dynamics of the tree Prosopis flexuosa change in cattle grazed areas compared to ungrazed areas in the Central Monte desert, Mendoza, Argentina. To this end, we quantified vital rates and constructed a population projection matrix model to compare the deterministic population growth rate (λ) between grazed and ungrazed areas. We also estimated elasticities of vital rates to evaluate their potential importance for future changes in λ and performed a life table response experiment (LTRE) to identify the life cycle transitions that contribute the most to the observed differences in λ between the two treatments. Although we found differences in demographic processes, such as lower seed production and higher probability of reversion to smaller size classes in young individuals when cattle were present, our results indicate that cattle grazing had no significant effect on λ for this species. According to the elasticity analysis, survival of large trees is the main driver of the population growth rate (λ) of P. flexuosa, and the vital rates related to tree reproduction, such as seed production and germination, have a poor contribution to λ. Therefore, limitations of activities that can affect survival of large trees should be considered as part of the conservation strategy for this species. Our study provides a compilation of demographic information that can be useful to set policies connecting the conservation objectives for this woodlands with that of ranch managers of the area.

Serial Dissection of Parasite Gene Families.

Calcium ion signaling regulates central aspects of the biology controlling stage and life cycle transitions of apicomplexan parasites. In the current issue of Infection and Immunity, Long and coworkers (S. Long, Q. Wang, and L. D. Sibley, Infect Immun 84:1262-1273, 2016, http://dx.doi.org/10.1128/IAI.01173-15) describe a powerful genetic system enabling reliable serial genetic dissection of a large gene family encoding novel calcium-dependent protein kinases (CDPKs) that provides new insights into the roles of CDPKs during Toxoplasma gondii infection.

A Plasmodium yoelii Mei2-Like RNA Binding Protein Is Essential for Completion of Liver Stage Schizogony.

Plasmodium parasites employ posttranscriptional regulatory mechanisms as their life cycle transitions between host cell invasion and replication within both the mosquito vector and mammalian host. RNA binding proteins (RBPs) provide one mechanism for modulation of RNA function. To explore the role of Plasmodium RBPs during parasite replication, we searched for RBPs that might play a role during liver stage development, the parasite stage that exhibits the most extensive growth and replication. We identified a parasite ortholog of the Mei2 (Meiosis inhibited 2) RBP that is conserved among Plasmodium species (PlasMei2) and exclusively transcribed in liver stage parasites. Epitope-tagged Plasmodium yoelii PlasMei2 was expressed only during liver stage schizogony and showed an apparent granular cytoplasmic location. Knockout of PlasMei2 (plasmei2(-)) in P. yoelii only affected late liver stage development. The P. yoelii plasmei2(-) liver stage size increased progressively until late in development, similar to wild-type parasite development. However, P. yoelii plasmei2(-) liver stage schizonts exhibited an abnormal DNA segregation phenotype and failed to form exoerythrocytic merozoites. Consequently the cellular integrity of P. yoelii plasmei2(-) liver stages became increasingly compromised late in development and the majority of P. yoelii plasmei2(-) underwent cell death by the time wild-type liver stages mature and release merozoites. This resulted in a complete block of P. yoelii plasmei2(-) transition from liver stage to blood stage infection in mice. Our results show for the first time the importance of a Plasmodium RBP in the coordinated progression of late liver stage schizogony and maturation of new invasive forms.

Moss development: Starting BELL for embryos.

Major life cycle transitions happen after changes in stem cells trigger new developmental programs. In moss, expression of the homeobox transcription factor BELL1 is sufficient to induce sporophyte stem cells from the gametophyte phase, without having to go through fertilization.

Secreted Cyclic Di-GMP Induces Stalk Cell Differentiation in the Eukaryote Dictyostelium discoideum.

Cyclic di-GMP (c-di-GMP) is currently recognized as the most widely used intracellular signal molecule in prokaryotes, but roles in eukaryotes were only recently discovered. In the social amoeba Dictyostelium discoideum, c-di-GMP, produced by a prokaryote-type diguanylate cyclase, induces the differentiation of stalk cells, thereby enabling the formation of spore-bearing fruiting bodies. In this review, we summarize the currently known mechanisms that control the major life cycle transitions of Dictyostelium and focus particularly on the role of c-di-GMP in stalk formation. Stalk cell differentiation has characteristics of autophagic cell death, a process that also occurs in higher eukaryotes. We discuss the respective roles of c-di-GMP and of another signal molecule, differentiation-inducing factor 1, in autophagic cell death in vitro and in stalk formation in vivo.

SAS-4 Protein in Trypanosoma brucei Controls Life Cycle Transitions by Modulating the Length of the Flagellum Attachment Zone Filament

The evolutionarily conserved centriole/basal body protein SAS-4 regulates centriole duplication in metazoa and basal body duplication in flagellated and ciliated organisms. Here, we report that the SAS-4 homolog in the flagellated protozoan Trypanosoma brucei, TbSAS-4, plays an unusual role in controlling life cycle transitions by regulating the length of the flagellum attachment zone (FAZ) filament, a specialized cytoskeletal structure required for flagellum adhesion and cell morphogenesis. TbSAS-4 is concentrated at the distal tip of the FAZ filament, and depletion of TbSAS-4 in the trypomastigote form disrupts the elongation of the new FAZ filament, generating cells with a shorter FAZ associated with a longer unattached flagellum and repositioned kinetoplast and basal body, reminiscent of epimastigote-like morphology. Further, we show that TbSAS-4 associates with six additional FAZ tip proteins, and depletion of TbSAS-4 disrupts the enrichment of these FAZ tip proteins at the new FAZ tip, suggesting a role of TbSAS-4 in maintaining the integrity of this FAZ tip protein complex. Together, these results uncover a novel function of TbSAS-4 in regulating the length of the FAZ filament to control basal body positioning and life cycle transitions in T. brucei.

Stochastic modelling of perfect inspection and repair actions for leak–failure prone internal corroded pipelines

To enhance the performance of any facility, reduce cost and failure probability involves proper inspection and repair decisions. To be able to establish the cost of repair and inspection of corroded pipelines at different stages of the corrosion defect depth growth, Markov modelling technique was adopted. This model formulated an inspection and repair technique, which has the potentials of aiding policy makers in maintenance management of internally corroded pipelines. The transition states were determined using the Remaining Useful Life (RUL) of the pipelines whilst Weibull distribution was used for calculating the corrosion wastage rates at the lifecycle transition phases. Monte Carlo simulation and degradation models were applied for determining future corrosion defect depth growth, in a bid to establish periodic inspection and repair procedures and their costs. Data from an onshore pipeline inspected with Magnetic Flux Leakage (MFL) in-Line-Inspection (ILI) technique was used to test the validity of the model. The results obtained indicate that the model has practical applications for inspection and repairs of aged-internally corroded pipelines.

Cross-reactive acquired immunity influences transmission success of the Lyme disease pathogen, Borrelia afzelii

Cross-reactive acquired immunity in the vertebrate host induces indirect competition between strains of a given pathogen species and is critical for understanding the ecology of mixed infections. In vector-borne diseases, cross-reactive antibodies can reduce pathogen transmission at the vector-to-host and the host-to-vector lifecycle transition. The highly polymorphic, immunodominant, outer surface protein C (OspC) of the tick-borne spirochete bacterium Borrelia afzelii induces a strong antibody response in the vertebrate host. To test how cross-immunity in the vertebrate host influences tick-to-host and host-to-tick transmission, mice were immunized with one of two strain-specific recombinant OspC proteins (A3, A10), challenged via tick bite with one of the two B. afzelii ospC strains (A3, A10), and infested with xenodiagnostic ticks. Immunization with a given rOspC antigen protected mice against homologous strains carrying the same major ospC group allele but provided little or no cross-protection against heterologous strains carrying a different major ospC group allele. There were cross-immunity effects on the tick spirochete load but not on the probability of host-to-tick transmission. The spirochete load in ticks that had fed on mice with cross-immune experience was reduced by a factor of two compared to ticks that had fed on naive control mice. In addition, strain-specific differences in mouse spirochete load, host-to-tick transmission, tick spirochete load, and the OspC-specific IgG response revealed the mechanisms that determine variation in transmission success between strains of B. afzelii. This study shows that cross-immunity in infected vertebrate hosts can reduce pathogen load in the arthropod vector with potential consequences for vector-to-host pathogen transmission.

The Kundum Festival in Ghana

Abstract During the Kundum festival in Ghana, people ritually express their wishes for productive farming and fishing seasons, social progress, spiritual protection, and a healthy natural environment, bringing into play the nonhumans expected to uphold or oppose these objectives. This article contributes to an improved analysis of the perspectives of intersubjectivity, relationship, and community in African rituals by examining the interaction between nonhumans and humans in the Kundum festival. It analyzes some of the transitional rites that constitute Kundum. These rites are less the markers of life-cycle or seasonal transitions than the very cultural resources by which transitions are put into effect in creating hope for the fertility of farmlands and rivers. They are linked to the creation of a purified food production season, to healing, to the roles assigned to nonhumans in the initiation of the festival, and to a configuration of relationships in a shared world.

Corporate Life-Cycle Dynamics of Cash Holdings

This paper shows that the corporate life-cycle is an important dimension for the dynamics and valuations of cash holdings. Our results indicate that firms’ cash policies are markedly interacted with their strategy choices. While firms in early stages and post-maturity stages hold large amounts of cash, cash ratios decrease when firms move towards maturity. Much of this variation in cash holdings is attributable to a changing demand function for cash over the different life-cycle stages. Trade-off and pecking order motives are of different importance for cash policies dependent on a firm’s life-cycle stage. An additional dollar in cash is highly valuable for introduction and growth firms, while a dollar in cash adds, on average, less than a dollar in market value for firms in later life-cycle stages, most likely due to increasing agency problems. Most of the dynamics in cash holdings are observed at life-cycle transition points rather than during the different life-cycle stages. Finally, the secular trend in cash holdings seems strongly attributable to increases in cash in the introduction and the decline stage.

Myoinhibitory peptide regulates feeding in the marine annelid Platynereis.

BackgroundDuring larval settlement and metamorphosis, marine invertebrates undergo changes in habitat, morphology, behavior and physiology. This change between life-cycle stages is often associated with a change in diet or a transition between a non-feeding and a feeding form. How larvae regulate changes in feeding during this life-cycle transition is not well understood. Neuropeptides are known to regulate several aspects of feeding, such as food search, ingestion and digestion. The marine annelid Platynereis dumerilii has a complex life cycle with a pelagic non-feeding larval stage and a benthic feeding postlarval stage, linked by the process of settlement. The conserved neuropeptide myoinhibitory peptide (MIP) is a key regulator of larval settlement behavior in Platynereis. Whether MIP also regulates the initiation of feeding, another aspect of the pelagic-to-benthic transition in Platynereis, is currently unknown.ResultsHere, we explore the contribution of MIP to the regulation of feeding behavior in settled Platynereis postlarvae. We find that in addition to expression in the brain, MIP is expressed in the gut of developing larvae in sensory neurons that densely innervate the hindgut, the foregut, and the midgut. Activating MIP signaling by synthetic neuropeptide addition causes increased gut peristalsis and more frequent pharynx extensions leading to increased food intake. Conversely, morpholino-mediated knockdown of MIP expression inhibits feeding. In the long-term, treatment of Platynereis postlarvae with synthetic MIP increases growth rate and results in earlier cephalic metamorphosis.ConclusionsOur results show that MIP activates ingestion and gut peristalsis in Platynereis postlarvae. MIP is expressed in enteroendocrine cells of the digestive system suggesting that following larval settlement, feeding may be initiated by a direct sensory-neurosecretory mechanism. This is similar to the mechanism by which MIP induces larval settlement. The pleiotropic roles of MIP may thus have evolved by redeploying the same signaling mechanism in different aspects of a life-cycle transition.Electronic supplementary materialThe online version of this article (doi:10.1186/s12983-014-0093-6) contains supplementary material, which is available to authorized users.