This research seeks to comprehensively assess the water footprint and environmental sustainability of millet-based ethanol production in India, responding to the growing interest in resilient and nutritious alternatives. Utilizing a life cycle assessment approach, the study will scrutinize water usage across the entire production process, from millet cultivation to ethanol extraction. The primary aim is to gain an in-depth understanding of water consumption at each stage, pinpointing areas for enhancement and efficiency. Rajasthan exhibited the highest total water footprint at 6596 m3/ton, primarily due to its substantial ETc value, while Andhra Pradesh showcased the lowest at 1906 m3/ton, owing to its high average yield. Haryana displayed significant water usage during crop production, with a water footprint of 5870 m3/ton, where WFgreen, WFblue, and WFgrey contributed 3730 m3/ton, 2010 m3/ton and 130 m3/ton respectively. Uttar Pradesh demonstrated a comparatively lower total water footprint of 2564 m3/ton. These findings are anticipated to offer valuable insights into the viability and sustainability of millet-derived ethanol as an eco-friendly biofuel option in India, guiding policymakers, researchers, and stakeholders in promoting its adoption and addressing environmental challenges linked with conventional fuel sources.