Abstract This paper presents new records of 135 lichen taxa in Bolivia. Two species, Myriotrema laeviusculum (Nyl.) Hale and Graphis tenoriensis Chaves & Lücking, are new for the Southern Hemisphere; two, Cladonia uncialis (L.) Weber ex F. H. Wigg. and Thelocarpon laureri (Flot.) Nyl., are new for South America; and 27 species are new for Bolivia: Acanthotrema brasilianum (Hale) Frisch, Bulbothrix laevigatula (Nyl.) Hale, B. leprieurii Aubel, Canoparmelia salacinifera (Hale) Hale, Catolechia wahlenbergii (Flot. ex Ach.) Körb., Chapsa alborosella (Nyl.) Frisch, Coccocarpia dissecta Swinscow & Krog, Graphis apertoinspersa Rivas Plata & Lücking, G. cinerea Fée, G. globosa (Fée) Spreng., G. haleana R. C. Harris, G. marginata Raddi, G. pilarensis Cáceres & Lücking, G. striatula (Ach.) Spreng., Hemithecium oryzaeforme (Fée) Staiger, Hypotrachyna protochlorina Sipman, Elix & T. H. Nash, H. sinuosella Elix, T. H. Nash & Sipman, H. tariensis Elix, Parmeliella isidiopannosa Jørg., Parmotrema conferendum Hale, P. enteroxanthum Hale, P. laciniellum (Ferraro & Elix) Blanco et al., P. pilosum (Stizenb.) Krog & Swinscow, P. recipiendum (Nyl.) Hale, P. ruptum (Lynge) Hale ex DePriest & Hale, Pyxine pungens Zahlbr. and Xanthoparmelia substenophylloides Hale. Notes on distribution are provided for the species. Bulbothrix goebelii (Zenker) Hale is excluded from the list of Bolivian lichens; the record is referred to B. laevigatula.