Lichen Genus Research Articles

Habitat type controls microarthropod community changes across a Magellanic sub-Antarctic elevation gradient

IntroductionElevation gradients are often used as a proxy for climate change as they allow comparisons of ecological responses over much larger temporal and spatial scales than is possible through experimental manipulations.MethodsHere, we tested how microarthropod communities (Collembola and Acari) are affected by climatic differences between sea level and 600 m a.s.l. on Navarino Island, in the Magellanic sub-Antarctic ecoregion of southern Chile (mean annual temperatures of 5.6 vs 3.1°C, respectively). We quantified microarthropod abundance, richness and community trait characteristics in dominant moss (Racomitrium lanuginosum and Polytrichum strictum) and lichen (Usnea trachycarpa, Pseudocyphellaria freycinetii and Stereocaulon alpinum) vegetation growing at both elevations. These moss and lichen genera are characterized by large morphological differences and allow testing of how habitat characteristics affect microarthropod community response across elevation gradients.ResultsCollembola and Acari community composition differed between the low and high elevation sites. Total abundance levels of Acari were maintained in each habitat across elevation, whereas Collembola richness strongly declined (50%) at high elevation in the moss habitats. Acari community differences across elevation were driven by relative abundance changes whereas the Collembola community lost species at higher elevation. An anticipated decline of smaller eudaphic Collembola at high elevation was only observed in the moss Racomitrium, reflecting potentially lower temperature buffering capacity and shelter options compared to Polytrichum. Lichens mostly supported larger epigeic species irrespective of elevation. There were no consistent patterns linking microarthropod communities with habitat water holding capacity or water loss rates across the studied habitats and elevation.DiscussionHabitat type and the genus of moss or lichen were associated with microarthropod community changes across elevation, including examples of declines, increases and no change. These findings highlight that community responses across gradients may not always relate to the generally hypothesized environmental variables (e.g. temperature variability) and that habitat characteristics should be taken into account when using elevation as a proxy for climate change.

The application of haplotypes instead of species-level ranks modifies the interpretation of ecological preferences in lichen symbiont interactions in Parmelia

The analysis of the interaction between main bionts (mycobiont and photobiont) in the lichen symbiosis delivers substantial information about their preferences in the selection of symbiotic partners, and their ecological preferences. The selectivity in the Parmelia genus has been defined as strong so far. However, data on this lichen genus, which includes several widely distributed species, are biogeographically limited. Therefore, using specialization indicators and extended sampling, in this study, we estimated the interactions between the main bionts of selected Parmelia spp., using two levels of estimation (species/OTU and haplotype). A comparison of mycobiont-photobiont interactions at different levels showed that considering only mycobiont species and Trebouxia OTUs, greater specialization is found, while Parmelia species studied in this work present a more generalistic strategy in photobiont choice when haplotypes are considered. Despite the uneven sampling of Parmelia species, the interpretation of specialization within species and individuals of the genus leads to a more precise and accurate interpretation of their adaptation strategies. Furthermore, the data from P. sulcata indicate the existence of a different pool of compatible haplotypes in some geographical regions compared to neighboring areas. This observation suggests the potential influence of climatic factors.

Three New Species and a New Record of the Lichen Genus Peltula (Peltulaceae) from Helan Mountain in China.

In this study, a systematic taxonomic analysis was carried out on the lichen genus Peltula, collected from Helan Mountain in China; three new species (Peltula helanense, P. overlappine, and P. reticulata) and a new record (P. crispatula (Nyl.) Egea) for China were identified. Four species were identified by morph-anatomical, chemical, and phylogenetic analyses by combining two gene loci (ITS and LSU). Peltula helanense is with tiny individual thalli up to 1mm, attached by creamy-white cylindrical rhizoids and apothecia filling the whole squamule. Peltula overlappine is characterized by thallus squamulose forming rosette-shaped patches and squamules with distinctive thickened margins. Peltula reticulata is characterized by brownish brown thallus and squamules with densely reticulate upper surface. P. crispatula is characterized by irregular squamules attached to a tuft of hyphae. The four species are described in detail, compared, and discussed with similar species, and images of morpho-anatomical structures of the four species are also provided. Moreover, a key to the species of Peltula from Helan Mountain is provided. The results enrich the data of the genus Peltula and also indicate that the rich diversity of lichen species in Helan Mountain is worthy of in-depth study.

A phylogenetic study of Micareamelaeniza and similar-looking species (Pilocarpaceae) unveils hidden diversity and clarifies species boundaries and reproduction modes.

Micarea (Ascomycota, Pilocarpaceae) is a large cosmopolitan genus of crustose lichens. We investigated molecular systematics and taxonomy of the poorly known Micareamelaeniza group focussing on M.melaeniza, M.nigella and M.osloensis. A total of 54 new sequences were generated and using Bayesian and maximum likelihood analysis of two markers (nuITS and mtSSU), we discovered two previously unrecognized phylogenetic lineages, one of which is described here as Micareaeurasiatica Kantelinen & G. Thor, sp. nov., morphologically characterized by pycnidia that are sessile to emergent, cylindrically shaped, with greenish-black K+ olive green, wall pigmentation and containing large mesoconidia up to 6 µm in length. The species is known from Japan and Finland. In addition, we show that the reproduction biology of M.osloensis has been poorly understood and that the species often occurs as an anamorph with stipitate pycnidia. We present a species synopsis and notes on pigments. Our research supports previous results of asexuality being an important reproductive strategy of species growing on dead wood.

New species and additional records in the lichen genus Malmidea from India

New species and additional records in the lichen genus <i>Malmidea</i> from India

Species of Peltula (lichenized Ascomycota) are shown to tolerate rock substrate rich in natural γ-radiation in NE Brazil

Pedra Municipality in Pernambuco State (Northeastern Brazil) is known for encompassing areas with high natural radiation from 238U sources, contaminating soil, atmosphere, water, vegetation, and animals, including rural activities. A lichen survey was performed in the area, and species belonging to 19 genera were identified. The diversity is considered very low when compared to nearby municipalities and other similar regions, which might indicate the effect of radiation on the environment. The lichen genus viz. Peltula seems uranium-resistant since its three species: Peltula obscurans (Nyl.) Gyeln., Peltula euploca (Ach.) Poelt and Peltula impressa (Vain.) Swinscow & Krog were the only ones found on rocks rich in this radionuclide. In this study, we show the first report of the occurrence of different Peltula species on anomalous rocks rich in natural radionuclides. In addition, it is the first official reference of P. obscurans and P. impressa to Pernambuco state. The confirmation of the preference of Peltula for substrata radionuclides-rich can indicate this genus as a radioactivity bioindicator.

Phylogeny of the genus Loxospora s.l. (Sarrameanales, Lecanoromycetes, Ascomycota), with Chicitaea gen. nov. and five new combinations in Chicitaea and Loxospora.

Loxospora is a genus of crustose lichens containing 13 accepted species that can be separated into two groups, based on differences in secondary chemistry that correlate with differences in characters of the sexual reproductive structures (asci and ascospores). Molecular phylogenetic analyses recovered these groups as monophyletic and support their recognition as distinct genera that differ in phenotypic characters. Species containing 2'-O-methylperlatolic acid are transferred to the new genus, Chicitaea Guzow-Krzem., Kukwa & Lendemer and four new combinations are proposed: C.assateaguensis (Lendemer) Guzow-Krzem., Kukwa & Lendemer, C.confusa (Lendemer) Guzow-Krzem., Kukwa & Lendemer, C.cristinae (Guzow-Krzem., Łubek, Kubiak & Kukwa) Guzow-Krzem., Kukwa & Lendemer and C.lecanoriformis (Lumbsch, A.W. Archer & Elix) Guzow-Krzem., Kukwa & Lendemer. The remaining species produce thamnolic acid and represent Loxospora s.str. Haplotype analyses recovered sequences of L.elatina in two distinct groups, one corresponding to L.elatina s.str. and one to Pertusariachloropolia, the latter being resurrected from synonymy of L.elatina and, thus, requiring the combination, L.chloropolia (Erichsen) Ptach-Styn, Guzow-Krzem., Tønsberg & Kukwa. Sequences of L.ochrophaea were found to be intermixed within the otherwise monophyletic L.elatina s.str. These two taxa, which differ in contrasting reproductive mode and overall geographic distributions, are maintained as distinct, pending further studies with additional molecular loci. Lectotypes are selected for Lecanoraelatina, Pertusariachloropolia and P.chloropoliaf.cana. The latter is a synonym of Loxosporachloropolia. New primers for the amplification of mtSSU are also presented.

The typification of Variolaria discoidea Pers.

The type species of the lichen genus Variolaria is V. discoidea. The name is solely based on a Hoffmann (1784) illustration. To stabilize the nomenclature, we have designated a lectotype and an epitype from Germany, for which we generated molecular data. The species was formerly placed in the genus Pertusaria as P. albescens, and is presently correctly named Lepra albescens.

Pseudomonas syringae isolated in lichens for the first time: Unveiling Peltigera genus as the exclusive host.

Pseudomonas syringae is a bacterial complex that is widespread through a range of environments, typically associated with plants where it can be pathogenic, but also found in non-plant environments such as clouds, precipitation, and surface waters. Understanding its distribution within the environment, and the habitats it occupies, is important for examining its evolution and understanding behaviours. After a recent study found P. syringae living among a range of vascular plant species in Iceland, we questioned whether lichens could harbour P. syringae. Sixteen different species of lichens were sampled all over Iceland, but only one lichen genus, Peltigera, was found to consistently harbour P. syringae. Phylogenetic analyses of P. syringae from 10 sampling points where lichen, tracheophyte, and/or moss were simultaneously collected showed significant differences between sampling points, but not between different plants and lichens from the same point. Furthermore, while there were similarities in the P. syringae population in tracheophytes and Peltigera, the densities in Peltigera thalli were lower than in moss and tracheophyte samples. This discovery suggests P. syringae strains can localize and survive in organisms beyond higher plants, and thus reveals opportunities for studying their influence on P. syringae evolution.

A New Species and Two New Records of the Lichen Genus Fissurina from China

The lichenized fungal genus Fissurina with mostly slit-like lirellae, belongs to Graphidaceae and is mainly distributed in tropical and subtropical regions. A total of 17 Fissurina species have been reported from China. During a survey of the lichen diversity of southern China, a new species Fissurina wuyinensis K.J. Shi, Z.F. Jia and X. Zhao, sp. nov. was found, which is characterized by a corticolous thallus without detected secondary substances, uncarbonized lirellae, and an exposed disc with pruina, muriform and amyloid ascospores. Furthermore, two new records of F. pseudostromatica, F. subcomparimuralis have been identified by morphological, anatomical, chemical and molecular studies. Phylogenetic analyses of three loci (ITS, nuLSU and mtSSU) supported the position of these species within Fissurina. Detailed morphological descriptions as well as high-resolution photographs of the morphology and anatomy of the three species are provided, as well as a comparison and discussion of the characteristics of similar species. The studied specimens were deposited in the Fungarium of the College of Life Sciences, Liaocheng University (LCUF).

Molecular and taxonomic studies on some Acarospora (Acarosporales, Ascomycota) species in Türkiye

Acarospora is a crustose lichen genus in the family Acarosporaceae and has a wide distribution. While the lichen genus Acarospora has more than 200 species in the world, the number of species so far determined in Türkiye is 41. Here we report three Acarospora species: A. irregularis H. Magn., A. rosulata (Th. Fr.) H. Magn., A. thamnina (Tuck.) Herre and two lichenicolous fungal species: Lichenostigma svandae Vondrák &amp; Šoun, Stigmidium fuscatae (Arnold) R. Sant. new to Türkiye. Detailed information on these 5 taxa is provided along with photographs. The nrITS, β tubulin and mtSSU gene regions of the new Acarospora records are studied and their phylogenetical positions are discussed.

Frequency, Abundance and Distribution of Lichen In National Botanical Garden, Mirpur, Dhaka, Bangladesh

An investigation was carried out to study lichen flora of National Botanical Garden, Dhaka, during the tenure of May 2018 to April 2019. Seven genera of lichens namely Chrysothrix, Cladonia, Cryptothecia, Herpothallon, Parmelia, Pertusaria and Usnea were recorded from 15 blocks of the Garden. Lichens were abundantly grown and their number increased from June to October, whereas from November to April the number of lichens gradually decreased. The maximum frequency percentage (36.52) of the association found in Parmelia while minimum frequency percentage was recorded 2.48 in Usnea. Among three different heights, the highest number of lichens was recorded within 2 to 4 feet. Most exclusively, Parmelia was found in all the studied blocks, whereas other identified lichens were observed randomly. The present investigation will be helpful to enrich the knowledge on lichen biota of Bangladesh. Dhaka Univ. J. Biol. Sci. 31(2): 273-280, 2022 (July)

Four New Records of the Lichen Genus Fissurina from China

Four New Records of the Lichen Genus Fissurina from China

A NEW SPECIES OF LABROCARPON (ASTERINALES), A LICHENICOLOUS ASCOMYCOTA WITH SUBMURIFORM ASCOSPORES FROM INDIA

During lichenicolous fungal studies in India, lichen genus Ochrolechia was found infected with a black lirellate fungus. Critical microscopical examination of the fungus revealed that it is a new species of Labrocarpon, which differs from L. canariense in having narrow exciple (15.0–20.0 µm vs 20.0–50.0 µm thick), 4-spored asci, 1-septate to submuriform, constricted ascospores with larger l/w value [(2.5–)2.9–3.4–3.9(–4.5) vs (1.9–)2.1–2.5(–2.8)], habitat preference (corticolous vs saxicolous) and host selection (Ochrolechia vs Pertusaria). The new species Labrocarpon submuriforme is described in detail.

Investigation of Dyeing Properties of Mohair Fiber Dyed with Natural Dyes Obtained from Candelariella reflexa

ABSTRACT The current study reports on using ascorbic acid as a possible substitute for improving fastness properties of natural dyes. In the mordanting process, microwave energy, which is a part of the sustainable and ecological production approach, was used. Renewable natural dye source Candelariella reflexa, which is a genus of lichen, was obtained from the trunk of Pinus nigra. Mohair fiber was dyed with natural dye extracted from Candelariella reflexa by using a conventional method. Before dyeing, mohair fiber was subjected to the premordanted process with iron (III) chloride (FeCl3) using microwave energy. In order to determine the effect of mordanting process parameters on dyeing properties, the mordanting process was performed with different concentrations and durations. In the dyeing process, ascorbic acid was added at different concentrations in the dyeing bath to improve the light fastness of samples. After the dyeing process, spectrophotometric features, light, and rubbing and washing fastness of samples were investigated. The color strength, washing, light, and rubbing fastness of dyed mohair fiber improve slightly with the premordanting process and by adding ascorbic acid. The spectrophotometric measurement results show that color coordinates vary from the mordanting time and amount of ascorbic acid. Furthermore, the use of microwave energy in the mordanting process leads to saving of energy and time. Besides, in this study, a machine learning-based model exploiting the artificial neural network (ANN) was developed for prediction of dyeing properties of mohair fiber dyed with natural dyes obtained from Candelariella reflexa. Experimental data obtained through various tests were first used to feed the proposed ANN, and then the trained ANN was validated and tested for the aim of prediction. The study results show that the proposed model can successfully predict most of the dyeing properties of mohair fiber. Therefore, this model can be used as an effective tool to estimate dyeing characteristics of mohair fiber.

Usnea in West Java: a potential source of bioactive secondary metabolites

Lichen is a symbiotic organism consisting of algae (photobionts) and fungi (mycobiont). In Indonesia, Usnea is the only genus of lichen that has been widely recognized and used by the local community for traditional medicine. It is locally known as kayu angin, rusuk angin, or janggot Kai. Furthermore, it produces lichen acid and other compounds as secondary metabolites that need investigation. Therefore, this study aimed to identify species and determine the secondary metabolites of Usnea in West Java. There were 13 species of Usnea identified, with U. rubrotincta, U. mutabilis, and U. barbata being new to West Java. The extraction of U. esperantiana has the highest number of lichen acids, with 20 different types identified. This study showed that Usnea contains lichen acid, flavonoid, and saponin compounds. The secondary metabolites are a potential source of natural products for bioprospecting.

Species diversity, systematic revision and molecular phylogeny of Ganodermataceae (Polyporales, Basidiomycota) with an emphasis on Chinese collections.

Ganodermataceae is one of the main families of macrofungi since species in the family are both ecologically and economically important. The double-walled basidiospores with ornamented endospore walls are the characteristic features of Ganodermataceae. It is a large and complex family; although many studies have focused on Ganodermataceae, the global diversity, geographic distribution, taxonomy and molecular phylogeny of Ganodermataceae still remained incompletely understood. In this work, taxonomic and phylogenetic studies on worldwide species of Ganodermataceae were carried out by morphological examination and molecular phylogenetic analyses inferred from six gene loci including the internal transcribed spacer regions (ITS), the large subunit of nuclear ribosomal RNA gene (nLSU), the second largest subunit of RNA polymerase II gene (rpb2), the translation elongation factor 1-α gene (tef1), the small subunit mitochondrial rRNA gene (mtSSU) and the small subunit nuclear ribosomal RNA gene (nSSU). A total of 1 382 sequences were used in the phylogenetic analyses, of which 817 were newly generated, including 132 sequences of ITS, 139 sequences of nLSU, 83 sequences of rpb2, 124 sequences of tef1, 150 sequences of mtSSU and 189 sequences of nSSU. The combined six-gene dataset included sequences from 391 specimens representing 146 taxa from Ganodermataceae. Based on morphological and phylogenetic analyses, 14 genera were confirmed in Ganodermataceae: Amauroderma, Amaurodermellus, Cristataspora, Foraminispora, Furtadoella, Ganoderma, Haddowia, Humphreya, Magoderna, Neoganoderma, Sanguinoderma, Sinoganoderma, Tomophagus and Trachydermella. Among these genera, Neoganoderma gen. nov. is proposed for Ganoderma neurosporum; Sinoganoderma gen. nov. is proposed for Ganoderma shandongense; Furtadoella gen. nov. is proposed to include taxa previously belonging to Furtadoa since Furtadoa is a homonym of a plant genus in the Araceae; Trachydermella gen. nov. is proposed to include Trachyderma tsunodae since Trachyderma is a homonym of a lichen genus in the Pannariaceae. Twenty-three new species, viz., Ganoderma acaciicola, G. acontextum, G. alpinum, G. bubalinomarginatum, G. castaneum, G. chuxiongense, G. cocoicola, G. fallax, G. guangxiense, G. puerense, G. subangustisporum, G. subellipsoideum, G. subflexipes, G. sublobatum, G. tongshanense, G. yunlingense, Haddowia macropora, Sanguinoderma guangdongense, Sa. infundibulare, Sa. longistipitum, Sa. melanocarpum, Sa. microsporum and Sa. tricolor are described. In addition, another 33 known species are also described in detail for comparison. Scanning electron micrographs of basidiospores of 10 genera in Ganodermataceae are provided. A key to the accepted genera of Ganodermataceae and keys to the accepted species of Ganoderma, Haddowia, Humphreya, Magoderna, Sanguinoderma and Tomophagus are also provided. In total, 278 species are accepted as members of Ganodermataceae including 59 species distributed in China. Taxonomic novelties: New genera: Furtadoella B.K. Cui & Y.F. Sun, Neoganoderma B.K. Cui & Y.F. Sun, Sinoganoderma B.K. Cui, J.H. Xing & Y.F. Sun and Trachydermella B.K. Cui & Y.F. Sun; New species: Ganoderma acaciicola B.K. Cui, J.H. Xing & Y.F. Sun, G. acontextum B.K. Cui, J.H. Xing & Vlasák, G. alpinum B.K. Cui, J.H. Xing & Y.F. Sun, G. bubalinomarginatum B.K. Cui, J.H. Xing & Y.F. Sun, G. castaneum B.K. Cui, J.H. Xing & Y.F. Sun, G. chuxiongense B.K. Cui, J.H. Xing & Y.F. Sun, G. cocoicola B.K. Cui, J.H. Xing & Y.F. Sun, G. fallax B.K. Cui, J.H. Xing & Vlasák, G. guangxiense B.K. Cui, J.H. Xing & Y.F. Sun, G. puerense B.K. Cui, J.H. Xing & Y.F. Sun, G. subangustisporum B.K. Cui, J.H. Xing & Y.F. Sun, G. subellipsoideum B.K. Cui, J.H. Xing & Y.F. Sun, G. subflexipes B.K. Cui, J.H. Xing & Y.F. Sun, G. sublobatum B.K. Cui, J.H. Xing & Y.F. Sun, G. tongshanense B.K. Cui, J.H. Xing & Y.F. Sun, G. yunlingense B.K. Cui, J.H. Xing & Y.F. Sun, Haddowia macropora B.K. Cui, Vlasák & Y.F. Sun, Sanguinoderma guangdongense B.K. Cui & Y.F. Sun, Sa. infundibulare B.K. Cui & Y.F. Sun, Sa. longistipitum B.K. Cui & Y.F. Sun, Sa. melanocarpum B.K. Cui & Y.F. Sun, Sa. microsporum B.K. Cui & Y.F. Sun and Sa. tricolor B.K. Cui & Y.F. Sun; New combinations: Furtadoella biseptata (Costa-Rezende et al.) B.K. Cui & Y.F. Sun, Fu. brasiliensis (Singer) B.K. Cui & Y.F. Sun, Fu. corneri (Gulaid & Ryvarden) B.K. Cui & Y.F. Sun, Neoganoderma neurosporum (J.S. Furtado) B.K. Cui & Y.F. Sun, Sinoganoderma shandongense (J.D. Zhao & L.W. Xu) B.K. Cui, J.H. Xing & Y.F. Sun and Trachydermella tsunodae (Yasuda ex Lloyd) B.K. Cui & Y.F. Sun. Citation: Sun Y-F, Xing J-H, He X-L, Wu D-M, Song C-G, Liu S, Vlasák J, Gates G, Gibertoni TB, Cui B-K (2022). Species diversity, systematic revision and molecular phylogeny of Ganodermataceae (Polyporales, Basidiomycota) with an emphasis on Chinese collections. Studies in Mycology 101: 287-415. doi: 10.3114/sim.2022.101.05.

An acid deficient population of Lambiella psephota from Antarctica and a new combination in the genus from Campbell Island with a world-wide key to the genus

Only two species of the lichen genus Lambiella are known from Antarctica: L. impavida and L. psephota. Here we report a new chemotype of L. psephota collected from James Ross Island in the North-East Antarctic Peninsula region. nrITS, mtSSU and RPB1 gene regions of the norstictic acid deficient L. psephota were obtained, which showed the new chemotype phylogenetically belongs to L. psephota. We also transfer Rimularia maculata to Lambiella, and provide an identification key of the 17 known species of Lambiella world-wide

Potential of Usnic Acid Compound from Lichen Genus Usnea sp. as Antidiabetic Agents.

Lichen Usnea sp. is potential as a new natural medicine. This study report isolation of secondary metabolites from lichen Usnea sp. and α-glucosidase inhibitory, which is potential as an antidiabetic agent. Lichen powder was macerated using methanol, separated using column chromatography gravity and thin-layer chromatography. The crystalline was isolated and purified by the recrystallization process for obtaining pure compound. The isolated compound was determined using FTIR and NMR spectroscopy (1H and 13C). The results showed that the isolated compound was yellow needle crystals. Based on the spectra data interpretation, it was obtained usnic acid compound with the molecular formula of C18H16O7. The α-glucosidase inhibitory activity test showed that the usnic acid had activity in inhibiting the α-glucosidase enzyme with an IC50 value of 106.78 µg/mL. The usnic acid from Usnea sp. has a very good impact on the source of natural compounds as an antidiabetic drug in the future.

Bactrospora totonacae (Arthoniales, Ascomycota), una nueva especie de Veracruz, México

Antecedentes y Objetivos: Bactrospora es un género de líquenes costrosos que se caracteriza por el color negro, aspecto verrucoso a denticulado y pequeño de sus apotecios, posee esporas mayoritariamente aciculares y en ocasiones muriformes, pero siempre más largas que anchas. El género está representado por 38 especies aceptadas. El objetivo de este trabajo es describir Bactrospora totonacae, una especie nueva del bosque tropical seco de Veracruz, México.Métodos: Los especímenes fueron recolectados en el “Cerro de los Metates”, municipio Actopan, Veracruz, México. Se realizaron pruebas macroquímicas, reacción UV y cromatografía en capa fina; asimismo, con la solución de Lugol se verificó la presencia de sustancias amiloides distribuidas en el talo y el ascoma. Las muestras estudiadas se depositaron en el herbario XALU de la Facultad de Biología de la Universidad Veracruzana.Resultados clave: La nueva especie Bactrospora totonacae se caracteriza por presentar pruina K + magenta rojizo en el disco. Conclusiones: La especie que se describe en Bactrospora es única porque la combinación de pruina K + magenta rojizo y un excípulo carbonizado y grueso no había sido reportada en alguna otra especie de este género.