The article presents the results of field studies in 2021–2022 and revision of the lichenological collection of A.V. Gudovicheva collected in the period from 2000 to 2021. The list of lichen flora of the Tula Region is supplemented with 34 taxa: 28 lichens, 2 allied saprotrophic fungi, and 4 lichenicolous fungi. Cladonia conista, Lecanora sarcopidoides, Lichenodiplis lichenicola, Marchandiomyces corallinus and Pyrenodesmia chalybaea are listed for the first time for the territory of Central Russia (within the Central Federal District). Bactrospora dryina, Chaenotheca hispidula, Cladonia parasitica and Cresponea chloroconia, as indicators of old-growth intact forest communities, are recommended to be included in the list of rare and vulnerable lichen species. The populations of these species need constant monitoring and control in the Tula Region.