Library Members Research Articles

Single-round deoxyribozyme discovery.

Artificial evolution experiments typically use libraries of ∼1015 sequences and require multiple rounds of selection to identify rare variants with a desired activity. Based on the simple structures of some aptamers and nucleic acid enzymes, we hypothesized that functional motifs could be isolated from significantly smaller libraries in a single round of selection followed by high-throughput sequencing. To test this idea, we investigated the catalytic potential of DNA architectures in which twelve or fifteen randomized positions were embedded in a scaffold present in all library members. After incubating in either the presence or absence of lead (which promotes the nonenzymatic cleavage of RNA), library members that cleaved themselves at an RNA linkage were purified by PAGE and characterized by high-throughput sequencing. These selections yielded deoxyribozymes with activities 8- to 30-fold lower than those previously isolated under similar conditions from libraries containing 1014 different sequences, indicating that the disadvantage of using a less diverse pool can be surprisingly small. It was also possible to elucidate the sequence requirements and secondary structures of deoxyribozymes without performing additional experiments. Due to its relative simplicity, we anticipate that this approach will accelerate the discovery of new catalytic DNA and RNA motifs.

Monitoring “Real-Time” Biological Binding Events on a Microelectrode Array

Because microelectrode arrays contain collections of spatially isolated electrodes that can each be individually addressed, they provide an intriguing platform for monitoring the activity of small molecules, peptides, and proteins. The arrays are utilized by placing the members of a molecular library on the surface of the array above the electrodes such that each unique member of the library is located on top of a unique electrode or a set of electrodes in the library. In this way, the binding activity on the molecular library and the electrochemical reactions happen on the electrodes are coupled. The binding events between the molecules on the surface of the array and molecules in solution can thus be monitored with the change of behavior of the electrochemical reactions on the electrodes. The overall strategy is illustrated in Figure below.We are expanding the types of experiments that can be employed to examine enzyme-peptide binding with the use of microelectrode array. We have previously shown RGD-peptide/ integrin, v-107/ VEGF, and R6A - Gαi1 interactions can be monitored on an array. While success of the synthetic chemistry can be ensured by fluorescence labeling, the binding can be monitored by cyclic voltammogram.Now, our focus has moved from distinguishing specific binding/non-specific binding/no binding to distinguishing different types of specific binding events. We are working to employ those electrochemical methods in conjunction with the traditional FRET studies in order to provide unique insights into the mechanism of peptide cleavage reactions. In so doing, we hope to add new capabilities to existing surface analytical methods. Figure 1

New Reductive Mediators for Expanding Array-Based Parallel Synthesis

Microelectrode arrays are powerful analytical tools for monitoring interactions between biological molecules in real-time. By placing members of a molecular library at defined electrodes, one can easily screen interactions with biological targets in the supernatant through simple electrochemical impedance experiments. However, one of the limitations to using arrays with very high electrode densities is the requirement of functionalizing each individual electrode (commonly over 12500/cm2) with a member of the molecular library to be tested. The Moeller Lab seeks to bypass this road block by developing synthetic tools that would allow for synthesis of the combinatorial library directly on the array surface through parallel synthesis techniques. One of the key features of any parallel synthesis strategy is protecting group orthogonality. The ability to selectively cleave one protecting group in the presence of various other protecting group moieties allows for diversification of a core scaffold in a site-specific manner. To this end, the Moeller Lab has utilized electrochemical methods to site-selectively generate acids, bases, Ce(IV), Ru(VII), Os(VIII), Cr(VI), Pd(0), Pd(II), Cu(I), Cu(II), quinones, H2, and Sc(III) at defined electrodes on a microelectrode array. In order to expand this toolset further, we are investigating protecting groups that are reductively cleavable, such as p-nitrobenzyl carbonates and carbamates. The reduction potential of these groups is readily tunable by modifying the substitution on the aromatic ring, giving rise to a small library of cleavable groups with different reduction potentials. One of the challenges towards using these groups on the arrays, however, is selection of an appropriate reductive mediator to shuttle electrons from the electrode at the array surface to the substrates localized on the outside of a polymer coating over the array. While oxidative mediators are relatively well-characterized and easy to come by, reductive mediators often require multistep syntheses to obtain and are less known than their oxidative counterparts. We hypothesize that these reductively cleavable protecting groups can be mediated by the aryl ring moiety present in the protecting group (e.g. p-nitrobenzene for a p-nitrobenzyl carbamate-protected amine). Addition of an alkyl substituent (such as the methylene in the carbamate) to an aryl ring makes the reduction potential of the aryl ring more negative by ~200 mV. Thus the free aryl ring should be able to serve as the mediator for reductive cleavage of the protecting group. In support of this hypothesis, we have shown that we can deprotect a p-nitrobenzyl carbamate-protected amine site-selectively on a microelectrode array using p-nitrobenzene as the mediator. In addition, CV studies of a p-nitrobenzyl-protected amine substrate show that p-nitrobenzene is able to transfer electrons to the substrate -- the reduction wave of the mediator disappears when substrate is added. Thus by optimizing these mediated reactions on the microelectrode arrays and in solution, we hope to introduce a new family of reductive mediators and protecting groups to the synthetic community. Figure 1

Toward the assembly and characterization of an encoded library hit confirmation platform: Bead-Assisted Ligand Isolation Mass Spectrometry (BALI-MS)

The ability to predict chemical structure from DNA sequence has to date been a necessary cornerstone of DNA-encoded library technology. DNA-encoded libraries (DELs) are typically screened by immobilized affinity selection and enriched library members are identified by counting the number of times an individual compound’s sequence is observed in the resultant dataset. Those with high signal reads (DEL hits) are subsequently followed up through off-DNA synthesis of the predicted small molecule structures. However, hits followed-up in this manner often fail to translate to confirmed ligands. To address this low conversion rate of DEL hits to off-DNA ligands, we have developed an approach that eliminates the reliance on chemical structure prediction from DNA sequence. Here we describe our method of combining non-combinatorial resynthesis on-DNA following library procedures as a rapid means to assess the probable molecules attached to the DNA barcode. Furthermore, we apply our Bead-Assisted Ligand Isolation Mass Spectrometry (BALI-MS) technique to identify the true binders found within the mixtures of on-DNA synthesis products. Finally, we describe a Normalized Enrichment (NE) metric that allows for the quantitative assessment of affinity selection in these studies. We exemplify how this combined approach enables the identification of putative hit matter against a clinically relevant therapeutic target bisphosphoglycerate mutase, BPGM.

Triaging of DNA-Encoded Library Selection Results by High-Throughput Resynthesis of DNA-Conjugate and Affinity Selection Mass Spectrometry.

DNA encoded library (DEL) technology allows for rapid identification of novel small-molecule ligands and thus enables early-stage drug discovery. DEL technology is well-established, numerous cases of discovered hit molecules have been published, and the technology is widely employed throughout the pharmaceutical industry. Nonetheless, DEL selection results can be difficult to interpret, as library member enrichment may derive from not only desired products, but also DNA-conjugated byproducts and starting materials. Note that DELs are generally produced using split-and-pool combinatorial chemistry, and DNA-conjugated byproducts and starting materials cannot be removed from the library mixture. Herein, we describe a method for high-throughput parallel resynthesis of DNA-conjugated molecules such that byproducts, starting materials, and desired products are produced in a single pot, using the same chemical reactions and reagents as during library production. The low-complexity mixtures of DNA-conjugate are then assessed for protein binding by affinity selection mass spectrometry and the molecular weights of the binding ligands ascertained. This workflow is demonstrated to be a practical tool to triage and validate potential hits from DEL selection data.

PERANCANGAN SISTEM INFORMASI PERPUSTAKAAN BERBASIS WEB DI SMK PLUS PRATAMA ADI BANDUNG

The rapid development of the computer world that brings every individual or group of people will use information and communication technology in every activity. Likewise in the world of education, including libraries that utilize information and communication technology in managing the process of borrowing and returning library books. The benefits of making a web-based library information system include being able to save time where there is no need for manual recording, making it easier to find book data or library members, making it easier in making book loan reports and other reports and other benefits. The research method used is Research and Development (R&D). The making of software is done by a waterfall model consisting of: (1) needs analysis, (2) design, (3) implementation, and (4) testing. Then UML visual modeling is used which is the standardization of modeling language for object-oriented software development. The expected results of this study produce a web-based library information system using the YII Framework with MVC (Model View Controller) method in SMK Plus Pratama Adi Bandung that can solve existing problems. The formulation of the problem of this research is how to build a library information system in SMK Plus Pratama Adi Bandung so that it can present accurate and efficient information. The purpose of this research is to produce a library information system.

Penerapan Sistem Informasi Administrasi Perpustakaan Menggunakan Model Desain User Experience

The library at SMP N 5 Bandarlampung is a junior high school library that has library management staff and the number of book collections according to library standards and has adequate library facilities and infrastructure. Students and students are required to become members of the school library to support the learning process. However, the process of borrowing and returning books currently carried out is still conventional, that is, all library administration data collection in borrowing and returning books is still written in the book, and when looking for the required data, you must open the book page. Application of library administration information systems using this user experience design, users can better understand and easily use applications that are made to maximize the performance of library staff, school principals, and students in managing library administration. The design of the library administration information system using the user experience design at SMP Negeri 5 Bandarlampung using the user centered design method has 5 stages namely, Empethized, Define Problem Statements, Indentation, and Prototype in accordance with the needs of users in the library administration information system based on the user experience design undertaken. The results of testing the criteria for the DeLone and McLean Model criteria for the success of the information system were 84.31% with the criteria being Very Good .

Bioresorbable tyrosol‐derived poly(ester‐arylate)s with tunable properties

AbstractWe designed and developed a novel library of tyrosol‐derived poly(ester‐arylate)s that exhibit tunable chemical, thermal, mechanical, and degradative properties. To build the library, the diphenols 4‐hydroxyphenethyl 2‐(4‐hydroxyphenyl)acetate (HTy) and 4‐hydroxyphenethyl 3‐(4‐hydroxyphenyl)propanoate (DTy) are synthesized and subsequently polymerized with various diacids. Characterization of library members is performed in order to assess the impact of chemical structure on polymer properties. Specifically, the relative influence of diphenol pseudosymmetry versus asymmetry, diacid carbon chain length, and diacid bond rigidity on resulting properties is investigated. Diphenol choice greatly impacts resulting polymer thermal properties and processability. HTy‐containing polymers generally have lower melting temperatures compared to their DTy‐derived counterparts and are easier to quench in the amorphous phase. As a result, processing results in greater tunability for HTy‐derived polymers. One specific example was pHTy3, which increased its tensile modulus from 1 GPa to 3 GPa upon drawing. Diacid lengths and bond rigidity also significantly influence thermal, mechanical, and degradative properties. In all, members of this library can be synthesized efficiently, with high molecular weight and exhibit a wide range of properties, motivating future commercial translation.

Appraising the Current Status, Issues and Challenges of Library and Information Faculty Members in the Universities of Pakistan

During recent times, there is much discussion about the challenges associated with the present and future of library and information science (LIS) education in Pakistan. The present study is aimed to explore the current status, issues and challenges of LIS faculty member who are considered the pillar of academic programs. It develops our understanding of LIS educators’ characteristics by presenting key findings with regard to their designation, gender, academic qualification, teaching strategies and work activities. Various issues and challenges along with faculty development strategies were also explored. Data were collected by employing both quantitative and qualitative approaches. A profile of LIS educators (i.e. budgeted positions for faculty members in different cadres and number of actual incumbents, gender wise distribution and academic qualification) was made by collecting quantitative data through questionnaire. Whilst interviews were conducted with17 faculty members about their teaching strategies, work activities and the overall challenges confronted to LIS educators in the country. Various faculty development strategies suggested by the respondents are also reported. The findings presented in the paper provide a unique insight into the characteristics and challenges of Pakistani library and information educators and will help to understand the needs of this workforce.

Utilization of Online Learning Platforms by LIS Arab Faculty Members during the Coronavirus Outbreak

The goal of this study is to investigate the use of online learning platforms by Arab public universities during the Novel Coronavirus outbreak from the perspective of faculty members of library & information sciences. A quantitative research approach was used in the form of an online survey conducted in April 2020 on 121 members, 72 males, and 49 females. Platforms, such as Youtube, Whatsapp, presentation tube, Google classrooms, Zoom, Blackboard, and Facebook have been identified as the most commonly used online learning platforms. Students’ engagement and improvement of their digital literacy were the most significant reasons for using these platforms

The XPS library website: A resource for the XPS community including - The XPS library of information, XPS spectra-base having >70,000 monochromatic XPS spectra, and spectral data processor (SDP) v8.0 software

Surface chemical analysis by XPS is more than 60 years old. During the last 15 years ther-e has been an explosive growth in the publication of XPS peak-fits and interpretations. Unfortunately, these publications suffer from a variety of problems in reliability and usefulness because the new authors are not familiar enough with the science and application of XPS, chemical state assignments, and peak-fitting. Efforts to help new users include journal publications such as self-training guides for peak-fitting, international standards, collections of BEs, examples of peak-fitting, and more. These user guides are useful and are published in journals that are known to publish XPS information. The basics of XPS are taught at a few graduate level courses in universities and there are 3–5 day training courses at conferences, but the need is much greater. To help the international XPS community and the infrequent users of XPS to overcome these limitations, problems and needs, The XPS Library™ was formed as a non-profit institute to provide an on-line resource of XPS information, self-training material, free >70,000 monochromatic XPS reference spectra, free spectral data processing software, reference tables, links, examples of good and bad peak-fitting, and more. In 1986, this author began to build The XPS Spectra-Base™ by collecting and organizing detailed sets of “self-consistent” monochromatic XPS spectra from reference materials, natural crystals, man-made single crystals, and commercial products. The current number of individual spectra in digital form is >70,000 and the number of materials is >9,000. Each set of spectra (4–10) is stored inside individual data directories having the name of the material analyzed. Multiple data directories belonging to the same category are placed in a directory with the name of that category. Each category name is distinct and is based on the chemistry of the materials. Currently, “XPS Library” members must download individual sets of spectra or full directories that are stored in ZIP files. To view, peak-fit, export, analyze, overlay, process, or print the spectra provided by The XPS Spectra-Base the library member needs a software that can import or directly open spectra having: VAMAS-ISO 14,976 ASCII file format, other ASCII file formats (XY, XYZ), or the SDP binary file format. Most software from the instrument makers can import these types of spectra. The “Spectral Data Processor” (SDP™) v8.0 is a 32-bit Windows™ (v7−10 compatible) software is provided free of charge to library members. Using SDP v8 the user can: (a) access and open the raw or processed spectra downloaded from The XPS Spectra-Base, (b) save all processing results and labels to permanent memory, (c) completely reprocess, peak-fit, analyze, overlay, process, or print the spectra, (d) export or save the raw or processed spectra in VAMAS-ISO ASCII and other ASCII file formats, and (e) import spectra having VAMAS-ISO 14,976 ASCII file format and other ASCII file formats (XY, XYZ). Several thousand High Energy Resolution Auger Electron Spectra (HER-AES) from insulators, conductors, and native oxides will be added. All spectra are owned, copyrighted, and are the property of The XPS Library and are protected by a registered international copyright (TX 4−560-881).

Optimization of multi-site nicking mutagenesis for generation of large, user-defined combinatorial libraries.

Generating combinatorial libraries of specific sets of mutations are essential for addressing protein engineering questions involving contingency in molecular evolution, epistatic relationships between mutations, as well as functional antibody and enzyme engineering. Here we present optimization of a combinatorial mutagenesis method involving template-based nicking mutagenesis, which allows for the generation of libraries with >99% coverage for tens of thousands of user-defined variants. The non-optimized method resulted in low library coverage, which could be rationalized by a model of oligonucleotide annealing bias resulting from the nucleotide mismatch free-energy difference between mutagenic oligo and template. The optimized method mitigated this thermodynamic bias using longer primer sets and faster annealing conditions. Our updated method, applied to two antibody fragments, delivered between 99.0% (32451/32768 library members) to >99.9% coverage (32757/32768) for our desired libraries in 2 days and at an approximate 140-fold sequencing depth of coverage.

Synthetic Molecular Evolution to Identify Hemocompatible Antimicrobial Peptides Effective Against Drug-Resistant, Biofilm-Forming Bacteria

Previously, we identified antimicrobial peptides (AMPs) that retain activity in the presence of eukaryotic cells by using a synthetic molecular evolution (SME) based approach. We demonstrated that variation in the sequence of antimicrobial peptides selected from primary screening of iterative peptide library can lead to identification of peptides that have superior antimicrobial activity, solubility, and lower cytotoxicity against red blood cells (RBCs) and mammalian cells. D-CONGA, a variant obtained by rational modification of the consensus sequence of peptides selected from stringent screening of iterative, second-generation antimicrobial peptide library is highly effective in vitro against all ESKAPE pathogens in the presence of RBCs and serum. The peptide also has anti-biofilm activity in vitro and shows potency against gram-negative and gram-positive drug-resistant bacteria and their biofilms in relevant infected wound model in mice. We next designed and synthesized 13 variants of D-CONGA and characterized them for their bactericidal activity in the presence and absence of RBCs. Their cytotoxicity against mammalian fibroblast, WI-38, and hemolysis in RBC were also measured. The best D-CONGA variant, D-CONGA-Q7, was then tested against 14 clinical isolates of drug-resistant bacteria. The anti-microbial activity of the new peptide was compared with D-CONGA and eight conventional antibiotics from four different classes and was found to be superior to all. Following this, we designed and synthesized a third-generation peptide library based on D-CONGA-Q7. The library members are being screened against gram-positive and gram-negative bacteria in the presence of RBCs. The selected peptides sterilize broad spectrum bacteria at submicromolar concentrations. Hence, we have shown that using rational design and SME, we can improve AMPs with each generation and discover evolved peptides that have increasingly potent and relevant activity against MDR bacteria, compared to conventional antibiotic or other known AMPs.

Abstract PR002: Highly parallel knock-in targeting for genome engineering of cellular therapies

Abstract Genetically-engineered immune cell therapies have proven effective to treat some types of cancer, but most tumors still cannot be cured. Forward genetics with CRISPR loss-of-function screens in T cells have identified some mutations to enhance cellular therapies, however these methods do not yet take full advantage of the power of gain-of-function knock-in targeting to engineer cell functions. We developed a robust new platform to assess the functional effects of pools of knock-in constructs in parallel. We used high-efficiency nonviral gene editing to introduce large panels of candidate therapeutic knock-in constructs into a defined genome position in human T cells allowing us to compete the cells against each other and test which constructs enhance T cell function. High-throughput pooled screening of targeted cells identified distinct library members that promoted T cell fitness under various resting, stimulated and immunosuppressive in vitro conditions. Direct competition among adoptively transferred human T cells in immunodeficient mice revealed a subset of constructs that promoted in vivo accumulation of tumor infiltrating lymphocytes. Pooled knock-ins combined with single-cell sequencing also revealed high-dimensional cellular phenotypes induced by each construct ex vivo and in an in vivo tumor microenvironment. Overall, these studies demonstrate the power of pooled knock-in technology to discover and functionally characterize complex synthetic gene programs that can be written into targeted genome sites to generate more effective cellular therapies. This abstract is also being presented as PO073. Citation Format: Theodore L. Roth, Alexander Marson. Highly parallel knock-in targeting for genome engineering of cellular therapies [abstract]. In: Abstracts: AACR Virtual Special Conference: Tumor Immunology and Immunotherapy; 2020 Oct 19-20. Philadelphia (PA): AACR; Cancer Immunol Res 2021;9(2 Suppl):Abstract nr PR002.

WEB-BASED LIBRARY INFORMATION SYSTEM IN MADRASAH IBTIDAIYAH NEGERI SURAKARTA

Library is a primary supporting facility in any level of education spanning from elementary school, middle school and higher education i.e., university. The main aim of library is providing facility in which it can improve educational quality and allow the member to advance their knowledge from various reference sources. Managing book circulation is a crucial aspect for library in which it further increases effectivity for its business process such as returning and borrowing books. However, Madrasah Ibtidaiyah (MI) Negeri Surakarta does not have any such application system which can help its library management, as a result the library member cannot obtain have updated information related to the library collection. Currently, the school relies on manual procedure where the library staff records borrowing and returning books activities in a certain book log. Unfortunately, the manual procedure causes missing data and data consistency in the recording book. Therefore, research aim to design and build e-library. The e-library is a web-based information system that has specific features that can address the school problems. To realize objective, we adopt waterfall software development method to build the information system. We also evaluate our design by using black box testing to ensure its functionality. Results show that our developed system works as it is designed and can handle any request from user without causing any errors.

Glucose Binding Drives Reconfiguration of a Dynamic Library of Urea-Containing Metal-Organic Assemblies.

A bis-urea-functionalized ditopic subcomponent assembled with 2-formylpyridine and FeII , resulting in a dynamic library of metal-organic assemblies: an irregular FeII4 L6 structure and three FeII2 L3 stereoisomers: left- and right-handed helicates and a meso-structure. This library reconfigured in response to the addition of monosaccharide derivatives, which served as guests for specific library members, and the rate of saccharide mutarotation was also enhanced by the library. The (P) enantiomer of the FeII2 L3 helical structure bound β-D-glucose selectively over α-D-glucose. As a consequence, the library collapsed into the (P)-FeII2 L3 helicate following glucose addition. The α-D-glucose was likewise transformed into the β-D-anomer during equilibration and binding. Thus, β-D-glucose and (P)-3 amplified each other in the product mixture, as metal-organic and saccharide libraries geared together into a single equilibrating system.

Glucose Binding Drives Reconfiguration of a Dynamic Library of Urea‐Containing Metal–Organic Assemblies

AbstractA bis‐urea‐functionalized ditopic subcomponent assembled with 2‐formylpyridine and FeII, resulting in a dynamic library of metal–organic assemblies: an irregular FeII4L6 structure and three FeII2L3 stereoisomers: left‐ and right‐handed helicates and a meso‐structure. This library reconfigured in response to the addition of monosaccharide derivatives, which served as guests for specific library members, and the rate of saccharide mutarotation was also enhanced by the library. The (P) enantiomer of the FeII2L3 helical structure bound β‐D‐glucose selectively over α‐D‐glucose. As a consequence, the library collapsed into the (P)‐FeII2L3 helicate following glucose addition. The α‐D‐glucose was likewise transformed into the β‐D‐anomer during equilibration and binding. Thus, β‐D‐glucose and (P)‐3 amplified each other in the product mixture, as metal–organic and saccharide libraries geared together into a single equilibrating system.

APLIKASI CLIENT-SERVER UNTUK SISTEM INFORMASI KEPUSTAKAAN PADA PERPUSTAKAAN PEGADILAN NEGERI SIDIKALANG

This study aims to solve the problem of processing book data at the Sidikalang District Court Library, which currently does not have an information system to process library data such as book data, library member data, borrowing and returning books. In the absence of information regarding available books, the library leader, namely customer representative recreation, the Sidikalang District Court does not know exactly the type and number of books available so that it can hamper the development of the library. This problem is resolved by designing a client-server application for library information systems in libraries. Sidikalang District Court. With this library information system it is hoped that it can simplify the work of librarians, the system for recording book data and member data becomes more structured, fast and accurate, accelerates the process of searching for member data, book data, borrowing, and returning transaction data, and services to library members become more good.

Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries.

Current methods used for measuring amino acid side-chain reactivity lack the throughput needed to screen large chemical libraries for interactions across the proteome. Here we redesigned the workflow for activity-based protein profiling of reactive cysteine residues by using a smaller desthiobiotin-based probe, sample multiplexing, reduced protein starting amounts and software to boost data acquisition in real time on the mass spectrometer. Our method, streamlined cysteine activity-based protein profiling (SLC-ABPP), achieved a 42-fold improvement in sample throughput, corresponding to profiling library members at a depth of >8,000 reactive cysteine sites at 18 min per compound. We applied it to identify proteome-wide targets of covalent inhibitors to mutant Kirsten rat sarcoma (KRAS)G12C and Bruton's tyrosine kinase (BTK). In addition, we created a resource of cysteine reactivity to 285 electrophiles in three human cell lines, which includes >20,000 cysteines from >6,000 proteins per line. The goal of proteome-wide profiling of cysteine reactivity across thousand-member libraries under several cellular contexts is now within reach.

SIKAP GENERASI Z TERHADAP PROGRAM PERPUSTAKAAN DIGITAL i-BAGENDIT

This study aims to determine the knowledge, feelings, and actions of Generation Z about the i-Bagendit Digital Library. The scope of this study includes the attitudes of Generation Z using three indicators of Cognitive, Affective, and conative . The method used in this research is descriptive quantitative. The population in this study are members of the i-Bagendit Digital Library, amounting to 198 people, with a sample of 50 respondents including Generation Z active users of the i-Bagendit Digital Library. The data collection technique uses a digital questionnaire distributed online. The results of this study prove the existence of a positive attitude given by Generation Z users of the i-Bagendit Digital Library. In the cognitive aspect, the percentage figure is 61.7%, proving that Generation Z has sufficient knowledge about the i-Bagendit Digital Library. In the affective aspect, the percentage figure is 78%, proving that Generation Z has positive feelings about the i-Bagendit Digital Library. In the conative aspect, the percentage figure is 71.4%, proving that Generation Z has positive actions about the i-Bagendit Digital Library. In the future, it is hoped that the management of the i-Bagendit Digital Library can increase the quantity of digital collections, design displays, and interesting features.