Liaocheng City Research Articles

Characteristics and genesis of high-quality metasilicate mineral water in Liaocheng City, Shandong Province.

Drinking natural mineral water often contains minerals and trace elements essential for human beings, such as strontium and silicon. As people's quality of life improves, so do their requirements for drinking water. Therefore, it is crucial to identify and develop high-quality groundwater that is rich in metasilicate and other minerals. The aim of this study is to reveal the distribution pattern and causes of high-quality metasilicate-rich groundwater in Liaocheng City through scientific methods, so as to provide a theoretical basis for the rational development and protection of mineral water resources. The results shown that Dong'e County was the main concentration area of high-quality metasilicate mineral water in Liaocheng City. The main reason for its high concentration of metasilicic acid was due to water-rock interaction. There was bedrock fissure water in the underlying water supply rock groups in Dong'e County, and the reaction between the metasilicate minerals in the rock groups and water was the main source of metasilicic acid. In addition, the development of fissures in the area provided a good storage and conductivity system for the enrichment of metasilicic acid in groundwater. Based on the analysis of hydrogeological conditions and the mechanism of formation of enriched areas, the enriched areas were divided into developable areas, protected and restricted development areas, and restricted development areas. The results of the study can lay a theoretical foundation for the systematic and scientific development, utilization, and protection of high-quality metasilicate mineral water in Liaocheng City.

Trade-Off and Coordination between Development and Ecological Protection of Urban Agglomerations along Rivers: A Case Study of Urban Agglomerations in the Shandong Section of the Lower Yellow River

Urban development of clusters situated along rivers significantly affects the health of the river ecosystems, the quality of urban environments, and the overall well-being of local communities. Ecosystem service supply value (ESSV) measures the delivery of ecosystem goods and services within a specific timeframe in a particular area. Using the lower Yellow River urban agglomeration (Shandong section) as a case, we comprehensively applied land use structure and intensity change analysis, quantitative calculation of ESS, and geographical probe methods to unveil ESS and its mechanism of response to the spatio-temporal evolution of the intensity of land use in urban agglomeration along the river. The key results were as follows: (1) Over the past two decades, farmland and construction land areas have continued to decrease and increase, respectively, with the intensity of land use change being highest from 2005 to 2010. (2) ESS has continued to rise over the past 20 years, with the income in 2020 being 11.142 billion yuan, an increase of 31.13%. The “low-value areas” are mainly concentrated in Liaocheng City, Dezhou City, and Tai’an City, which are characterized by predominantly flat terrains where farmland constitutes the principal land use type. Conversely, “high-value areas” are largely in the counties bordering the Yellow River, including the upper estuary in the north and the rugged, southeastern terrains. (3) Areas with concentrated ESSV were primarily localized in the northern estuary area and along the Yellow River in a scattered point-like pattern. The spatial distribution of hotspots has become increasingly concentrated, transitioning from points to planes. Conversely, cold spots initially increased in number before subsequently decreasing. Waterbody was the most sensitive ESSV-determining factor. (4) The spatial heterogeneity of ESSV emerges as a consequence of the interaction of multiple factors, and among these interactions, those involving NDVI and POP contain the greatest explanatory power. Our findings are expected to offer a scientific foundation for optimizing land spatial patterns and enhancing ecological management in the lower Yellow River region.

Epidemiological characteristics and spatial aggregation of acute myocardial infarction in Shandong Province

Objective: To understand the characteristics and trends of acute myocardial infarction (AMI) in Shandong Province and to provide evidence for formulating prevention and control strategies. Methods: Data were derived from the AMI incidence reports of Shandong Province's Chronic Disease Surveillance Information Management System in 2012-2021. The crude and standardized incidence rates were used as indicators to describe the incidence level of AMI. Joinpoint regression analysis was used to analyze the trends in the incidence and age of onset over the years. The contribution of population aging to the increase in AMI incidence was assessed using the rate difference decomposition method. The incidence of AMI in each district (county) in Shandong Province was visualized using ArcGIS 10.8 software, and global and local spatial autocorrelation analysis was performed using DeoDa 1.12 software. Results: From 2012 to 2021, 198 233 cases of AMI were reported from 19 provincial monitoring sites in Shandong Province, of which 53.13% were males and 97.12% were ≥45 years old. The reported crude incidence increased from 90.12 per 100 000 in 2012 to 176.54 per 100 000 in 2021, with an average annual increase of 7.01% (Z=7.35, P<0.001). There was no significant upward trend in standardized incidence (Z=1.64, P=0.140), but the standardized incidence of male residents showed an increasing trend (Z=2.76, P=0.028). Before 2014, the reported crude incidence of males was similar to that of females, but after 2014, the reported crude incidence of males was continuously higher than that of females. However, males' standardized incidence was higher than females in all years. Both crude and standardized incidence rates were higher in rural residents than in urban areas. The median onset of AMI increased from 71.6 years old in 2012 to 73.5 years old in 2021. The median age of onset in males was lower than that in females in all years, and in most years, the median age of onset in urban residents was lower than that in rural residents. The incidence of AMI in males showed a trend in younger age groups. According to the seasonal decomposition, the incidence peak of AMI was in January, and the trough was in September. The contribution of aging population to the increase in crude incidence of AMI increased from 8.63% in 2013 to 52.58% in 2021. The global spatial autocorrelation analysis showed that the incidence of AMI presented an obvious spatial clustering distribution. Local spatial autocorrelation analysis found that the high-incidence areas (counties) were mainly concentrated in Liaocheng City and Dezhou City in the northwest region of Shandong Province and Heze City in the southwest. Conclusions: The incidence of AMI among residents in Shandong Province was rising, with spatial clustering and seasonal clustering characteristics. People aged 45 years and older, male residents, and rural residents were at high risk of developing AMI. There was a certain trend of younger age at onset among men. Targeted prevention and control measures should be taken for high-incidence seasons, high-risk groups, and high-incidence clustering areas in northwestern Shandong Province.

Research on the protection of and renewal strategies for historic buildings in the ancient city of Liaocheng based on multisource data

ABSTRACT Historic buildings play an important role in studying the evolution of urban history. However, fast urbanisation has proposed challenges to maintaining its vitality. These challenges highlight the importance of identifying solutions for the revitalisation of historic buildings. Therefore, this paper aims to explore solutions for the revitalisation of historic buildings in the ancient city of Liaocheng. A comprehensive evaluation of the existing eight unused historic buildings was conducted using the building adaptive reuse potential method to determine the potential for renovation design and the priority of reuse. Simultaneously, the Reach value, core density, and correlation of supporting facilities within the ancient city were analysed based on GIS technology and urban network analysis tools. Based on the evaluation, strategies for functional transformation and optimisation of surrounding facilities are proposed to provide a reference plan for the sustainable development of historic buildings in the ancient city of Liaocheng.

First Report of Sugarcane Mosaic Virus Infecting Goose Grass in Shandong Province, China.

Sugarcane mosaic virus (SCMV genus Potyvirus, family Potyviridae) can infect maize, sugarcane, sorghum, other graminaceous crops, and some weed species (Alegria et al., 2003; Achon et al., 2007). In August 2023, the leaves of goose grass (Eleusine indica) plants surrounding maize fields in a village of Liaocheng City, Shandong Province, China showed mosaic and chlorotic symptoms (26%, 11 of 43 grasses; Figure S1). Three symptomatic goose grass samples were selected and pooled for total RNA isolation using TRIzol reagent (Tiangen, Beijing, China). A small RNA library was created using 2.0 μg of total RNA and the mirVana miRNA Isolation Kit, followed by size selection (18-28 nt), adapter ligation, purification, reverse transcription (RT), and polymerase chain reaction (PCR) enrichment. High-throughput sequencing (HTS) was then performed on a HiSeq 2500 platform (Illumina, San Diego, CA, USA). The adapter sequences were removed and the reads were assembled de novo into larger contigs using ABySS software v. 1.9.0 with a k-mer of 32. Fifty-one contigs were obtained after the reads were spliced and screened (alignment length > 30 bp; e-value ≤ 0.05). The contigs were compared with viral sequences in GenBank using local BLASTn. Thirty-four contigs (34-64 nt) had the highest identities (97.18-100%) with the SCMV genome sequence, covering approximately 12.8% of the SCMV genome (Table S1). The low coverage of small contigs mapping to the SCMV genome in the HTS results may be attributed to variations in sequencing depth and sample preparation quality, biological aspects of the virus affecting siRNA production and detection, as well as the variability in viral genome and its size (Golyaev et al., 2019; Valenzuela et al., 2022). The other 17 contigs did not align to any plant virus sequences, but aligned to plant sequences, including Phragmites australis and Panicum virgatum. Potyvirus-degenerated primers PotyF (5'-ATGGTHTGGTGYATHGARAAYGG-3') and PotyR (5'-TGCTGCKGCYTTCATYTG-3') (Marie-Jeanne et al. 2000) were used in RT-PCR to detect SCMV in symptomatic leaves, yielding a ~300 bp amplicon. Sanger sequencing and BLASTn analysis confirmed the 97.98% nucleotide identity with SCMV isolate BJ (GenBank accession No. AY042184.1). The sequence was deposited in GenBank under accession number OR777055. In addition, specific SCMV primers SCMV-F (5'- TCCGGAACTGTGGATGCA-3') and SCMV-R (5'- GTGGTGCTGCTGCACTCCC-3') (coat protein region, 939 bp) detected the virus in all 11 symptomatic goose grass leaves, with no detection in asymptomatic leaves. Inoculation tests using extracts from symptomatic goose grass on maize plants resulted in mosaic symptoms (7 of 15 plants) at 4-6 days post-inoculation (Figure S2 and 3). However, no symptoms were observed in maize plants following inoculation with leaf extracts from healthy goose grass. RT-PCR confirmed the presence of SCMV in the diseased maize plants. Sequencing analysis revealed that all amplified fragments shared 100% identity with the partial CP-encoding sequence of SCMV. Taken together these results support the presence of SCMV in symptomatic goose grass. To the best of our knowledge, this is the first report of SCMV in E. indica in China. In general, potyviruses can be easily transmitted in multiple ways including aphid vectors, grafting, and wounding. Therefore, investigating SCMV in goose grass is crucial for developing integrated strategies to prevent its transmission to economically important plants such as maize.

Urban Flood Risk Assessment Based on a Combination of Subjective and Objective Multi-Weight Methods

Against the backdrop of global warming and rising sea levels coupled with increasing urbanization, flood risks for plain cities have intensified. This study takes Liaocheng City as its research object and constructs a regional flood risk assessment model based on a combination of subjective and objective multi-weight methods. The model sets weights according to different return periods from three perspectives: the severity of disaster-causing factors, the exposure of disaster-prone environments, and the vulnerability of disaster-bearing bodies. It also uses a subjective–objective combination of weights for the severity of disaster-causing factors, adopts CRITIC-entropy weights for the exposure of disaster-prone environments and vulnerability of disaster-bearing bodies, and adopts AHP subjective weights for the criterion layer. Based on GIS spatial analysis technology, the examination and zoning of flood disasters at a county scale were carried out. The results show that, unlike the existing weighting methods and machine learning methods, this multi-weight combination method can simultaneously avoid the subjectivity of the results and the uncertainty of parameters, thus enabling more accurate decision-making results to be obtained. The spatial distribution of the comprehensive risk is high in the central and western parts and relatively low in the south and north, while the area characterized by very high risk is concentrated in Dongchangfu District and Guanxian County. With the gradual increase in return periods, the overall spatial distribution of medium-to-very-high-risk areas in risk zoning gradually shrinks, and the spatial distribution of very-high-risk areas gradually moves south but maintains a stable distribution rule. Flood risk assessment is an important basic process for disaster prevention and mitigation in plain cities, and the results of this study can provide a reference for similar plain cities.

A Quantitative Study on the Intersection of Personal Competence and Classroom Efficiency in Dance Teaching: A Case of Middle and Primary Schools in Dongchangfu District, Liaocheng City, Shandong Province, China

Dance education plays a vital role in fostering creativity, self-confidence, and overall well-being among students. Effective dance instruction requires a deep understanding of the intersection between personal competence and classroom efficiency among dance teachers. However, this intersection remains relatively unexplored, particularly in the context of Dongchangfu District, Liaocheng City, Shandong Province, China. To address this gap, this quantitative study examines how dance teachers' personal competence influences classroom efficiency in terms of student engagement, learning outcomes, and overall teaching effectiveness. A sample of 93 dance teachers from 45 primary and secondary schools in Dongchangfu District participated in the study. The findings reveal that dance teachers generally perceive themselves as competent and effective in their teaching practices, with high levels of technical skills, pedagogical knowledge, adaptability in teaching methods, communication effectiveness, and engagement in professional development activities. Moreover, teachers reported high levels of classroom efficiency, including student participation and engagement, achievement of learning objectives, effectiveness of teaching methods, classroom behavior management, and overall satisfaction with teaching outcomes. The study underscores the importance of continuous professional development, student-centered instructional strategies, effective classroom management, and support for novice teachers in enhancing dance education practices. The implications of the findings for practice, policy, and future research are discussed.

Stress Analysis and Structural Optimization of Steel–Concrete Joint of Zhonghua Road Bridge in Liaocheng

Based on Zhonghua Road Bridge in Liaocheng city, a single tower hybrid cable-stayed bridge, the stress of the steelconcrete joint of the girder is studied using the finite element method with the software MIDAS and ANSYS. The beam element model of the whole bridge is established in MIDAS CIVIL, from which the internal forces of the critical sections of the steel–concrete joint under six adverse conditions are obtained. Then, the results are applied in the refined solid-shell finite element model established in ANSYS. With the stress analysis, it is found that when the steel–concrete joint is used in the girder of the single tower cable-stayed bridge, both the compressive strength of concrete and the tensile strength of steel can be fully used, and the structural stiffness can be smoothly transferred through the two different materials in the joint, which makes the structural behavior more reasonable. The detailed stress analysis also shows that it is prone to generate considerable stress concentration at the corners and the connection between the steel lattice chamber and the pressure bearing plate, and the concentration can be avoided by local stiffening and smoothing of the chamfers. The sensitivity analysis of the web thickness in the joints and bearing plates showed that increasing the thickness of the intermediate web would lead to a moderate reduction in the stress of the web itself, while the stress in the steel member remained relatively constant. However, when the thickness of the bearing plate is increased from 60 to 80 mm, the stress of each part of the steel plate in the steel grid will be significantly reduced, and the maximum reduction can reach 48%. The improvement of concrete strength has little effect on the stress of the steel cell, and the C50 concrete strength grade used in the design is more reasonable.

Epidemiological and comparative genomic analyses of Multidrug-Resistant Acinetobacter baumannii collected between 2020 and 2022 in Liaocheng City, Shandong Province, China

The emergence and prevalence of multidrug-resistant Acinetobacter baumannii (MRAB) poses a huge challenge to clinical treatment. To investigate the genetic and epidemiologic characteristics of MRAB in Liaocheng, China, and to explore potential resistance mechanisms, 56 MRAB strains were collected from the clinical departments of seven hospitals in Liaocheng between 2020 and 2022. Molecular typing, antimicrobial resistance patterns, and epidemiological characteristics were determined by genome sequencing and comparative genome analysis. Sequence type (ST) 540 was the most prevalent ST of the 56 MRAB in Liaocheng, and most strains (92.86%) were grouped into CC92. Core genome multilocus sequence typing subdivided the strains according to the number of allelic differences and could distinguish different outbreaks caused by ST540 isolates in the hospitals. All the isolates harbored blaOXA-23 and blaADC-25, and at least 92.86% of the isolates were resistant to 10 antibiotics. The major MRAB epidemic clone detected in Liaocheng was ST540, which was different from the results reported in other regions in China. Furthermore, several inter-hospital transmissions of ST540 isolates were observed. The findings highlight the urgent need for effective nosocomial infection control measures and the continuous surveillance of ST540 MRAB in Liaocheng.

Disaster Risk Regionalization and Prediction of Corn Thrips Combined with Cloud Model: A Case Study of Shandong Province, China

Corn thrips do serious harm to the yield and quality of corn. In this paper, the Shandong Province of China was taken as the study area. Based on the data of the occurrence of corn thrips in Shandong Province, a risk regionalization model was established by using eight indicators under four categories of hazard, sensitivity, vulnerability and the disaster prevention and mitigation capacity of diseases and pests on a monthly time scale. Firstly, the cloud model was introduced to determine the weight of each indicator, and then the risk regionalization of the corn thrips disaster in Shandong Province was carried out using the weighted percentage method, the weighted comprehensive evaluation method and the natural disaster risk index method. Finally, combined with the collected data, the disaster prediction of corn thrip occurrence degree was realized based on multiple linear regression, genetic algorithm optimized back-propagation neural network and genetic algorithm optimized support vector machine methods. The results show that: (1) the risk of Corn thrips disaster is mainly concentrated in the central and western parts of Shandong Province. Heze City is a high-risk area. Liaocheng City, Dezhou City, Jinan City and Weifang City are relatively high-risk areas. (2) By comparing the prediction accuracy of the three models, it was determined that the genetic algorithm optimized support vector machine model has the best effect, with an average accuracy of 79.984%, which is 7.013% and 22.745% higher than that of the multiple linear regression and genetic algorithm optimized back-propagation neural network methods, respectively. The results of this study can provide a scientific basis for fine prevention of corn thrips in Shandong Province.

东方白鹳在山东省适宜栖息地的分布预测

PDF HTML阅读 XML下载 导出引用 引用提醒 东方白鹳在山东省适宜栖息地的分布预测 DOI: 10.5846/stxb202009052308 作者: 作者单位: 作者简介: 通讯作者: 中图分类号: 基金项目: 云南省基础研究计划项目(20210AT070040,2019FD0070) Prediction of the suitable habitat distribution of Oriental White Stork in Shandong Province Author: Affiliation: Fund Project: 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:东方白鹳(Ciconia boyciana) 隶属鹳形目(Ciconiiformes)鹳科(Cioniidae)鹳属(Ciconia),被IUCN濒危物种红色名录列为濒危(EN),主要繁殖于俄罗斯远东地区与中国东北三江平原,在长江中下游鄱阳湖等沿海内陆湖泊越冬。山东省从2003年开始有东方白鹳繁殖记录,黄河三角洲为该物种在山东省的主要繁殖地。以在黄河三角洲自然保护区收集到的位点数据、物种分布数据库(GBIF)提取的位点数据和山东省环境气候因子为基础数据,基于最大熵(Maxent)模型和地理信息系统(ArcGIS)技术平台,预测东方白鹳在山东省的潜在适生区。结果表明在该物种在黄河三角洲及附近地区存在较集中的高适宜区,庙岛群岛和微山湖地区存在少量高适宜区,高适宜区总面积为1359.27km2;北至河北边界,南至微山县,沿黄河支流至西到达聊城市、菏泽市,存在1893.82km2较适宜区和3955.63km2低适宜区。潜在适宜生境总和为7208.72km2,占比为研究区域的0.89%。新出现的较为集中的较适宜区在微山湖周围地区,此地目前并无该物种出现的记录。通过对环境因子分析得知,东方白鹳选择适宜栖息地时更倾向于选择的植被类型是灌丛和草甸,土地利用类型是沼泽地、滩涂与河渠,远离道路,显著影响东方白鹳选择适宜区的气候因子是最暖季度降雨量和最干月份降雨量。研究旨在探究何种环境因子影响东方白鹳对适宜栖息地的选择,为该物种的栖息地管理和保护区建立提供科学依据,进而促进该物种种群和栖息地保护。 Abstract:The Oriental White Stork (Ciconia boyciana) belongs to the Ciconiiformes Stork family (Cioniidae) and the Stork genus (Ciconia). It is an endangered (EN) species on the International Union for Conservation of Nature (IUCN) Red List and is the national first-level protected wildlife. They mainly breed in the Far East of Russia and the Sanjiang Plain in Northeast China and overwinter in coastal inland lakes such as Poyang Lake in the middle and lower reaches of the Yangtze River. Shandong Province has recorded the reproduction of the Oriental White Stork since 2003. The Yellow River Delta is the main breeding ground for this species in Shandong Province. The purpose of this study is to explore what environmental factors affect the selection of suitable habitats for the Oriental White Stork. This article uses the site data collected in the Yellow River Delta Nature Reserve, the site data extracted from the Species Distribution Database (GBIF) and the environmental and climate factors of Shandong Province as the basic data, based on the Maximum Entropy (Maxent) model and geographic information system (ArcGIS), and predicts the potential suitable area of the Oriental White Stork in Shandong Province. The results show that the most suitable habitat for the species is concentrated in the Yellow River Delta and nearby areas. From the border of Hebei in the north of Shandong Province to Weishan County in the south, along the tributaries of the Yellow River to the west to Liaocheng City and Heze City, there are 3955.63 km2 low suitable areas and 1893.82 km2 more suitable area. The total potential suitable habitat is 7208.72 km2, of which the most suitable area is 1359.27 km2, accounting for 0.89% of the study area. The newly emerged more concentrated and more suitable patches are in the area around Weishan Lake, where there is currently no record of this species. Through the analysis of environmental factors, it is known that the vegetation types that the Oriental White Stork prefers to choose when choosing suitable habitats are shrubs and meadows, and the land use types are swamps, tidal flats and canals far away from roads. The climatic factors that significantly affect the Oriental White Stork's selection of suitable areas are the warmest quarter rainfall and the driest month rainfall. The results provide basic scientific evidence for the habitat management and establishment of the protected areas for this species, and promote the protection of the species' population and habitat. 参考文献 相似文献 引证文献

Optimization and Construction of Ecological Security Patterns Based on Natural and Cultivated Land Disturbance

In previous research on the construction of ecological security patterns (ESPs), the positioning characteristics of urban development were rarely considered, resulting in the identification of key conservation areas that are insufficient to support the ecological security of the entire region and the overall development of urban functions. Firstly, a “quality-importance-connectivity-balance” framework was created to identify ESPs and chose Liaocheng City (LC), a typical main agricultural production area, as the study site. Secondly, the ecological security level of the watershed perspective is an integrated resistance assessment method that exhibits topography, human activities, distance, and agricultural environmental impacts. Finally, the coordination pattern between agriculture and ecology was divided by the comprehensive quality of cultivated land and ESPs. A “six cores-seven belts-three zones” optimization pattern was constructed based on the components of ESPs and the distribution of cultivated land comprehensive quality. The study presents a novel approach for measuring ESPs and is an essential resource for ecological conservation and regional development planning in agroecologically complex regions.

First Report of Fusarium solani Species Complex Causing Vascular Wilt on Cauliflower in China.

Cauliflower (Brassica oleracea var. botrytis L.), which belongs to the family Cruciferae, is a cool-season vegetable with green leaves around a large hard white head of flowers. China is the leading cauliflower and broccoli producing country in the world, with approximately 10.71 MT production (FAOSTAT 2019). During September 2018 to July 2019, wilting symptoms were observed on cauliflower in several commercial fields, with approximately 45% to 65% disease incidence in Shen county (115°48'E, 35°98N) of Liaocheng city, Shandong province, China. Plant stunting, leaves yellowing and wilting, and dark brown, hollow appearance of vascular stem tissues were the symptoms prominently observed. To isolate the causal organism, nine symptomatic tissues were collected and cut into small pieces (5 × 5 mm), disinfected in 75% ethanol for 30 s, rinsed three times in sterile water, transferred onto potato dextrose agar (PDA) medium. The plates were then incubated in air-conditioned room at 26°C with an artificial 12 h light-dark cycle provided by incandescent lamp. In total, 15 single-spore isolates were obtained and morphological characterization of 15 isolates was done on both PDA and carnation leaf agar (CLA; Leslie and Summerell 2006). The mycelia on PDA were initially white, fluffy, later becoming brown, and the underside of the colonies were light brown. Typical macroconidia were abundant on CLA. Macroconidia were hyaline, slightly curved, one to five septa, both ends were smooth, measuring 3.7 to 6.4 μm × 23.7 to 38.1 μm (n = 40). Microconidia were oval to cylindrical, hyaline, zero to one septate, measuring 2.0 to 4.1 × 4.3 to 10.3 μm (n = 40). Chlamydospores were terminal or intercalary, solitary or in pairs, globose to oval, thick wall, smooth or rough, 6.3 to 9.8 μm. Based on morphological characteristics, all of the 15 isolates were identified as Fusarium solani (Leslie and Summerell 2006). The isolates were further identified based on PCR amplification. The ITS, mtSSU, EF-1α and RPB2 genes were amplified using primers ITS1/ITS4, NSM1/NSM2 (Li et al. 1994), EF-1 Ha/EF-2Tb (O'Donnell et al. 1998) and RPB2-5F2/fRPB2-7cR (O'Donnell et al. 2008). BLAST analysis showed that 15 isolates were highly similar to F. solani species complex, with 100% similarity for ITS (AB470904.1), mtSSU (KF125009.1), EF-1α (KF372878.1), and RPB2 (MK048113.1), respectively. The sequences of isolate HYC1410080102 had been deposited in GenBank with accessions MT378292.1 for ITS, MT383122.1 for mtSSU, OK595059.1 for EF-1α and OK595060.1 for RPB2, respectively. Pathogenicity of the 15 isolates were conducted on 4-true-leaf seedlings cv. Jinsong by dipping the roots into a conidial suspension (107 conidia/mL) for 10 min. The conidial was prepared from 7-day old cultures grown on CLA at 26°C and suspended in sterilized water. Control plants were dipped in sterile distilled water. All treated seedlings were planted in 5.0 cm diameter plastic pots containing pasteurized soil matrix. Then the plants were kept in a greenhouse at 15°C (night)/26°C (day) and 80%RH with natural daylight. Twelve days later, brown lesions appeared on stem bases in all inoculated cauliflowers, and finally, the plants wilted, similar to those observed in the field. The control plants remained healthy. Re-isolation of the infected tissues showed same morphological characteristics of F. solani as the original isolates, which were verified using PCR. To our knowledge, this is the first report of F. solani causing cauliflower wilt in China and the world (Farr and Rossman 2021). F. solani is a destructive pathogen with a broad host range worldwide and is responsible for significant crop losses, prevention and control measures should be considered.

The Causes Affecting the Parenting Stress of the Post-80s Generation in China with Working Mothers of Infants and Young Children as the Target

At present, people’s life pressure is increasing. In order to help alleviate the pressure of professional mothers’ upbringing, in this study, the treatment program for working mothers or parental education program was analyzed. A questionnaire survey was conducted among 278 mothers of male and female infants aged 0–5 in three kindergartens in Liaocheng City, Shandong Province. SPSS 23.0 software was used for frequency analysis, correlation analysis, and regression analysis. This study aims to improve the mood and self-esteem of working mothers with young children, and briefly introduce the policy of providing important support in addition to relatives. The results show that: first, the age of children is the cause of mother’s parenting pressure. The younger the children are, the greater the parenting pressure is; second, the higher the mother’s sense of parenting efficacy, the lower the parenting pressure; third, the higher the mother’s self-esteem, the lower the parenting pressure; fourth, the social support that working mothers receive may reduce their childcare pressure. Reducing the parental rearing pressure of working mothers with low self-esteem can help actively promote the positive development of their children. This article provides some reference for the relief of the pressure of professional mothers’ parenting.

Analysis of Chemical Composition Characteristics and Source of PM2.5 under Different Pollution Degrees in Autumn and Winter of Liaocheng, China

Analysis of chemical composition characteristics of PM2.5 under different pollution degrees can reveal the changes of pollution sources. In order to make clear the evolution process of PM2.5 compositions in autumn and winter, PM2.5 samples were continuously collected and analyzed at Liaocheng city, China. The collected samples were classified as clean days (CLD), mild-moderate pollution days (MMD) and severe-serious pollution days (SSD). It was concluded that with the increase of pollution degrees, the concentrations of water-soluble ions and carbon components increased significantly, while elements only increased slightly. In addition, as the pollution degrees increased, the percentage of NO3−, SO42− and NH4+ increased significantly, from 23.0% in CLD to 49.0% in SSD, while the percentage of other components decreased, especially crust material. The PMF analyzed results showed that secondary transformation (36.7%), combustion sources (20.4%), secondary organic aerosols (SOA) (11.7%), vehicle sources (11%), dust (10.5%) and industrial processes (9.7%) were the main sources of PM2.5 during autumn and winter in Liaocheng. The contribution of secondary transformation reached 57% at the SSD level, which indicated that it was the main reason for the increase of PM2.5 concentrations. The air mass mainly came from five paths to Liaocheng. The secondary transformation contribution of the air mass with short transmission distance was higher, while the contribution of the dust was higher from the long distance.

Spatial variation and influence factor analysis of soil heavy metal As based on geoDetector

Heavy metals in soil are closely related to our production and life. As heavy metal has relatively high toxicity, it is necessary to clarify its existence. This study took Liaocheng City as the study area. Sampling was carried out by the 10 km × 10 km grid center method and the concentration of heavy metal arsenic (As) at the sampling points was extracted. Based on the national secondary standard and Shandong background value, the single factor index was used to evaluate the status of heavy metal As in the soil of Liaocheng City, and eight factors related to heavy metal As were selected. This study used geographic detectors to identify spatial relationships among the factors. By the statistical description of the heavy metal As in the soil of Liaocheng City and the evaluation of the single factor index, we found that there was light pollution in most areas of Liaocheng City. By analyzing the results of the GeoDetector, it was found that the soil organic matter, soil subcategory, distance to river, and GDP were the dominant factors that affected the concentration and spatial variation of heavy metal As. The interaction results showed that the interaction between GDP and other influencing factors significantly increased the explanatory power of As.

A Preventive and Control Strategy for COVID-19 Infection: An Experience From a Third-Tier Chinese City.

COVID-19 is a rapidly spreading infectious disease that has led to a global pandemic. This study describes a novel strategy for preventing and controlling COVID-19 infection in the third-tier city of Liaocheng, China. The prevention and control measurements included city-wide orders to close workspaces, sanitize essential workspaces, quarantine individuals with a travel history to an epidemic area, and issue emergency medical responses to quarantine and treat COVID-19 patients using all necessary technologies, personnel, and resources. As a result, there were only 38 diagnosed COVID-19 cases in Liaocheng since the pandemic began in China in late 2019, including in the metropolitan area and six suburban counties, accounting for more than 6.39 millions residents living in a 8,715 km2 area. There was no COVID-19-related fatality and no healthcare professional inter-transmission as of June 25, 2020. The strategies of this third-tier Chinese city provide useful insights into approaches to prevent and control COVID-19 spread in other Chinese cities and countries.

Effects of long-term excessive iodine intake on blood lipids in Chinese adults: a cross-sectional study.

The aim of this study was to evaluate the effects of long-term excessive iodine intake on blood lipids in adults. Three counties from Dezhou city and Liaocheng city in the Shandong province of China were selected as survey locations. Three to five villages were selected from each county and then categorized by the iodine concentration detected in the groundwater into Low (<10 µg/L), Medium (10-150 µg/L), High (150-300 µg/L), and Excessive (>300 µg/L) groups. A self-reported questionnaire was completed by each subject to provide demographic characteristics. Body height, weight, and blood pressure were recorded by trained staff. Blood lipids were measured. A total of 2156 subjects were recruited for the final analysis. The serum triglyceride (TRIG) was significantly higher in the Excessive group than in the other three groups (P < 0.05). Total cholesterol (TCHOL) and low-density lipoprotein-cholesterol (LDL-C) showed downward trends with the increases in the water iodine concentration. A statistical significance of the crude correlation coefficient was detected between the water iodine concentration and the TRIG, TCHOL, or LDL-C (P < 0.05). A significant correlation was also noted between the water iodine concentration and TCHOL or LDL-C after adjustment for covariates. High iodine concentration was a significant protective factor for TCHOL and LDL-C in adults, whereas elevated BMI and advancing age were risk factors for both variables. An association was identified between iodine excess and low TCHOL or LDL-C. In areas with excessive iodine, iodine intake should be considered in studies examining the factors that influence blood lipids.

Associations between dietary patterns and physical fitness among Chinese elderly.

To explore the relationship between dietary patterns and physical fitness among older Chinese (≥60 years) individuals. Cross-sectional survey. Dietary data were collected by a simplified semi-quantitative FFQ. The 30-s Chair Stand test (30sCST), 30-s Arm Curl test (30sACT), 8-foot Time Up-and-Go test (8fTUAGT) and 6-min Walking test (6mWT) were used to assess physical fitness. Dietary patterns were obtained by factor analysis. The association between dietary patterns and physical fitness was explored by multiple logistic regression. Six communities (villages) of three districts in Liaocheng City (Shandong Province, China). A total of 596 residents were recruited from April to May 2017. Among 556 residents who were finally enrolled, 196 were men (35 %) and 360 were women (65 %). Three dietary patterns were identified: 'Western', 'Vegetarian' and 'Modern'. The 30sACT revealed that men in the fourth quartile of the Western pattern were less likely to be classified in the 'high-level' group, but men in the fourth quartile of the Vegetarian pattern were classified in the high-level group. The 6mWT revealed that men in the fourth quartile of the Modern pattern were classified in the high-level group. These associations were independent of confounding factors. Adherence to the Vegetarian pattern and Modern pattern may be protective factors for maintaining good physical fitness in older Chinese individuals. The Western pattern may lead to poor physical fitness in this population.

Research on the Payment Method of Medical Insurance—A Case Study of Chiping County in Liaocheng City

Since the reform and opening up, medical insurance system in China has made great achievements. As the focus and difficulty of medical insurance management and reform, the payment method of medical insurance has attracted increasing attention. Based on the semi-structured interview data of chiping county, the status quo and development trend of the payment method of medical insurance in China are discussed. It is found that the current payment method of medical insurance in China is mainly based on the payment by service items, which belongs to post payment system. The development trend of payment method is changing from single payment method to mixed payment method, changing from post payment system to prospective payment system, emphasizing and promoting payment by disease type and increasing the application of diagnosis related groups-prospective payment system. Late payment and insufficient payment restrict the payment method from post payment system to prospective payment system. The payment method of medical insurance determines the level of medical expenses, the flow of medical resources, the income of medical institutions and the quality of medical services, which will be one of the key factors affecting the success or failure of medical insurance system in China.