This study includes two newly synthesized prodrugs of Levofloxacin derivatives mono and dipeptide H2, where was the Synthesized monoPeptide as Levofloxacin-Histadine (L-H), as well as dipeptide H6 as Synthesis of diPeptide (phenylalanine- levofloxacin (GPA-L), using a Levofloxacin substituted. Spectroscopic data were studied for two derivative compounds H2 and H6 and reactivity indices were characterized using techniques FT-IR, 1H-NMR, and 13C-NMR. All the newly produced derivative compounds H2 and H6 have FT-IR spectra that share similarities in certain fingerprint-like bands and other bands. The essential functional group vibration bands. In the DMSO-d6 solvent, the compound H2, and H6 of 1H NMR spectra were studied, Typically, the fitted intensity ratio of the observed compound 1H NMR spectra gives, and the expected signals. The DMSO-d6 solvent was employed to record the 13C NMR spectra of levofloxacin derivatives H2 and H6. portrayed the 13C NMR spectra of generated compounds H1 through H6, comprised of the 13C NMR data. where done Further confirmation of the properties of the developed molecule emerged from 13C-NMR spectra. 1
 The bioactivity was studied for two derivative compounds H2 and H6 against three gram-positive and three gram-negative Bacteria. The mono and dipeptide prodrugs that are derivatives of levofloxacin have appeared to have excellent results against these Bacteria. Antibiotic susceptibility experimental findings revealed that various bacteria had taken different approaches to the tested drugs. most bacteria isolated in gram-positive and gram-negative demonstrated severe sensitivity to the generated compounds (H2 and H6). The spectral results in the FT-IR analyses as well as the nuclear magnetic resonance studies showed the high validity of the compounds formed ( H2 and H6) interaction, as well as the biological results showed a clear positive in killing bacteria of the type of Gram-negative and Gram-Positive.