Lever Arm Research Articles

Sequence Alignment-Based Prediction of Myosin 7A: Structural Implications and Protein Interactions.

Myosin, a superfamily of motor proteins, obtain the energy they require for movement from ATP hydrolysis to perform various functions by binding to actin filaments. Extensive studies have clarified the diverse functions performed by the different isoforms of myosin. However, the unavailability of resolved structures has made it difficult to understand the way in which their mechanochemical cycle and structural diversity give rise to distinct functional properties. With this study, we seek to further our understanding of the structural organization of the myosin 7A motor domain by modeling the tertiary structure of myosin 7A based on its primary sequence. Multiple sequence alignment and a comparison of the models of different myosin isoforms and myosin 7A not only enabled us to identify highly conserved nucleotide binding sites but also to predict actin binding sites. In addition, the actomyosin-7A complex was predicted from the protein-protein interaction model, from which the core interface sites of actin and the myosin 7A motor domain were defined. Finally, sequence alignment and the comparison of models were used to suggest the possibility of a pliant region existing between the converter domain and lever arm of myosin 7A. The results of this study provide insights into the structure of myosin 7A that could serve as a framework for higher resolution studies in future.

Surgical outcomes of anterior column reconstruction for spinal fractures caused by minor trauma-preoperative examination of the number of intervertebral bone bridges is key to obtaining good bone fusion-

BackgroundTo achieve good bone fusion in anterior column reconstruction for vertebral fractures, not only bone mineral density (BMD) and bone metabolism markers but also lever arms due to bone bridging between vertebral bodies should be evaluated. However, until now, no lever arm index has been devised. Therefore, we believe that the maximum number of vertebral bodies that are bony and cross-linked with the contiguous adjacent vertebrae (maxVB) can be used as a measure for lever arms. The purpose of this study is to investigate the surgical outcomes of anterior column reconstruction for spinal fractures and to determine the effect of bone bridging between vertebral bodies on the rate of bone fusion using the maxVB as an indicator of the length of the lever arm.MethodsThe clinical data of 81 patients who underwent anterior column reconstruction for spinal fracture between 2014 and 2022 were evaluated. The bone fusion rate, back pain score, between the maxVB = 0 and the maxVB ≥ 2 patients were adjusted for confounding factors (age, smoking history, diabetes mellitus history, BMD, osteoporosis drugs, surgical technique, number of fixed vertebrae, materials used for the anterior props, etc.) and analysed with multivariate or multiple regression analyses. The bone healing rate and incidence of postoperative back pain were compared among the three groups (maxVB = 0, 2\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$$\\leqq$$\\end{document}maxVB\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$$\\leqq$$\\end{document}8, maxVB ≧ 9) and divided by the maxVB after adjusting for confounding factors.ResultsPatients with a maxVB ≥ 2 had a significantly higher bone fusion rate (p < 0.01) and postoperative back pain score (p < 0.01) than those with a maxVB = 0. Among the three groups, the bone fusion rate and back pain score were significantly higher in the 2\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$$\\leqq$$\\end{document}maxVB\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$$\\leqq$$\\end{document}8 group (p = 0.01, p < 0.01).ConclusionsExamination of the maxVB as an indicator of the use of a lever arm is beneficial for anterior column reconstruction for vertebral fractures. Patients with no intervertebral bone bridging or a high number of bone bridges are in more need of measures to promote bone fusion than patients with a moderate number of bone bridges are.

Research on dynamic characteristics of cutter breaking frozen soil and optimal drum rotation speed of the new shaft tunneling machine

To improve the efficiency of frozen soil excavation, the new shaft tunneling machine was developed. The new shaft tunneling machine exerts pressure on the frozen soil through the cutter under the joint action of its own gravity, the drum rotational force and the inertia force, and the frozen soil is damaged. By unique way of breaking frozen soil to improve the efficiency of frozen soil excavation, the drum rotation speed is one of the factors affecting the performance of frozen soil excavation. This article applies SolidWorks software to establish the model of cutter breaking frozen soil, takes advantage of Hyper Mesh finite element software coupled with LS-DYNA solver to acquire the regular pattern of change in the force change, frozen soil stress–strain and specific energy of cutter crushing frozen soil, etc., which analyzes the destruction of frozen soil when the drum of the new shaft tunneling machine is rotating at the speed of 25–40 rpm. Combine with field test to investigate the mechanism of cutter breaking frozen soil under the optimal drum rotation speed. The investigation results demonstrate that: when frozen soil's self-bearing capacity is lower than the force of cutter, it breaks up and detaches from the soil body, and frozen soil undergoes tensile, compressive and shear damages. For this research, it is instructive for practical engineering.

Exponential decay of solutions to an inertial model for a wave equation with viscoelastic damping and time varying delay

In the present work, we study the global existence and uniform decay rates of solutions to the initial-boundary value problem related to the dynamic behavior of evolution equations accounting for rotational inertial forces along with a linear time-nonlocal Kelvin-Voigt damping arising in viscoelastic materials. By constructing appropriate Lyapunov functional, we show that the solution converges to the equilibrium state exponentially in the energy space.

The internal joint stabilizer for elbow instability: current concepts

The management of residual elbow instability is challenging in both acute and chronic injuries. Among the available devices, the hinged external fixator provides an additional joint stabilization while allowing an early motion, but it is clumsy and associated to high rate of pin track complications. To address these issues, an internal joint stabilizer (IJS) has been recently developed. An easier recreation of the axis of rotation coupled to the reduced lever arm of the hinge is the root of the consistent and satisfactory results thus far observed. In addition, the device is more comfortable for the patients being an internal stabilizer. Nonetheless, a second surgery for the device removal is necessary, of which the timing is still not standardized. This current concepts paper describes literature regarding outcomes of the IJS focusing on the rate of maintained radiographic joint reduction, the resultant range of motion, and the associated complication profile.

Low-cost, manual centrifuge for separation of particles from water

Water separation is a critical process with far-reaching implications for the environment, public health, and various industries. Traditional water filtration methods are often limited to commercial use, posing challenges for individuals lacking access to clean water, or the equipment to separate particles suspended in water. In this study, we developed a spinning device for water separation through centrifugal and rotation force. Our goal was to create a device that could provide a low-cost alternative for removing contaminants from water. During the testing, we found that the device had a theoretical efficiency of 67% and longer separation times consistently produced significantly lower absorbance values in the visible range, indicating more effective particle removal. A computational model was also used to simulate the movement of particles under the influence of rotational forces, and showed that the smallest particle in this experiment (microplastics) can be theoretically moved by the rotational forces. This study underscores the promise of a manually powered spinning toy as an innovative and efficient tool for water separation and contaminant removal, holding the potential to address global water quality challenges.

Infantile Acute Subdural Hematohygroma: A Clinical and Neuroimaging Analysis of 18 Cases

&amp;lt;i&amp;gt;Objective&amp;lt;/i&amp;gt;: Even after the advent of magnetic resonance imaging (MRI), few studies have focused on the clinical profiles and neuroimaging characteristics of acute subdural hematohygroma (ASDHy) in infants. The present study attempted to elucidate the pathogenesis of ASDHy by analyzing the clinical and neuroimaging findings of ASDHy. &amp;lt;i&amp;gt;Materials &amp; Methods&amp;lt;/i&amp;gt;: The subjects were 18 patients (eight males and ten females) younger than 2 years with ASDHy. Their median age at diagnosis was 3.8 months (range: 2-10 months), and 15 (83%) were younger than 4 months. Imaging studies found no evidence of any primary brain injury. &amp;lt;i&amp;gt;Results&amp;lt;/i&amp;gt;: Five patients experienced a fall, and 13 (72%) had no history of head trauma. The presenting symptoms and signs were the acute onset of generalized convulsive seizures and or repeated vomiting. Retinal hemorrhage occurred in 14 patients (78%). The imaging studies revealed a large amount of bilateral, subdural fluid collection with an intensity higher than that of cerebrospinal fluid on MRI. Benign enlargement of the subarachnoid space (BESS) was found in 14 patients (78%), an enlarged sylvian fissure (LSF) was found in all the patients (100%), and greater, left-sided enlargement was evident in 16 patients (89%). Two patients underwent subdural drainage, and 16 patients (89%) were conservatively managed. All the patients had a favorable outcome. &amp;lt;i&amp;gt;Conclusions&amp;lt;/i&amp;gt;: ASDHy is a self-limiting disorder associated with retinal hemorrhage and a benign clinical course. Most cases develop in early infancy. LSF presumably loosens the tight fixation of the temporal lobes at the middle cranial fossa, thereby increasing the rotational force of the brain even during mild or unnoticeable impacts. Increased cranio-cerebral disproportion, including BESS and LSF, is a structural vulnerability which can lead to ASDHy in early infancy.

Homologous mutations in human β, embryonic, and perinatal muscle myosins have divergent effects on molecular power generation

Mutations at a highly conserved homologous residue in three closely related muscle myosins cause three distinct diseases involving muscle defects: R671C in β-cardiac myosin causes hypertrophic cardiomyopathy, R672C and R672H in embryonic skeletal myosin cause Freeman-Sheldon syndrome, and R674Q in perinatal skeletal myosin causes trismus-pseudocamptodactyly syndrome. It is not known whether their effects at the molecular level are similar to one another or correlate with disease phenotype and severity. To this end, we investigated the effects of the homologous mutations on key factors of molecular power production using recombinantly expressed human β, embryonic, and perinatal myosin subfragment-1. We found large effects in the developmental myosins but minimal effects in β myosin, and magnitude of changes correlated partially with clinical severity. The mutations in the developmental myosins dramatically decreased the step size and load-sensitive actin-detachment rate of single molecules measured by optical tweezers, in addition to decreasing overall enzymatic (ATPase) cycle rate. In contrast, the only measured effect of R671C in β myosin was a larger step size. Our measurements of step size and bound times predicted velocities consistent with those measured in an in vitro motility assay. Finally, molecular dynamics simulations predicted that the arginine to cysteine mutation in embryonic, but not β, myosin may reduce pre-powerstroke lever arm priming and ADP pocket opening, providing a possible structural mechanism consistent with the experimental observations. This paper presents direct comparisons of homologous mutations in several different myosin isoforms, whose divergent functional effects are a testament to myosin's highly allosteric nature.

Effects of simulated radioulnar synostosis on supination and pronation in cats. A cadaveric study.

To evaluate the effect of an induced synostosis with a screw on pronation and supination in cats. Ex vivo biomechanical study. A total of 58 feline forelimbs. A total of 58 cadaveric feline thoracic limbs were mounted on a custom-built jig with the elbow and carpus flexed at a 90° angle. To exclude any orthopedic disease, radiographs of the forelimbs were performed prior to the mechanical tests. Radioulnar synostosis was imitated with a 2 mm cortical screw through the radius into the ulna in the proximal (Group P; n = 54), middle (Group M; n = 52), and distal (Group D; n = 53) radial diaphysis. The angles of pronation and supination were recorded after manually applying a two-finger tight rotational force to the metacarpus. Rotational tests were performed without a screw (Group N) and with a screw in each of the aforementioned positions. Pairwise comparisons between the groups were performed based on their angles of rotation with a paired t-test with the Benjamini-Hochberg procedure and a mixed model ANOVA. Mean angles of rotation decreased between Group N (129.5 ± 15.9°) and all groups with imitated radioulnar synostosis to a mean angle of 37.5 ± 14.5° (p < .0001). Mean angles of rotation did not differ between the groups with imitated radioulnar synostosis. Induced radioulnar synostosis decreases antebrachial rotation by more than two-thirds, regardless of location. Implants fixating the radius to the ulna should be avoided in cats, regardless where they are located along the radial diaphysis.

Economic Analysis and Feasibility of Self-propelled Rotor Power Weeder

The manual weeding process is laborious, requires significant effort, and is a costly operation. Farmers typically allocate 30 to 40 percent of their total crop production expenses to the weeding process. The research was carried out by development and economic feasibility of self-propelled weeder for small category farmers. Economic analysis and feasibility of developed mechanical weeder was evaluated by considering cost of operation, breakeven point and payback period. The utilization of a self-propelled weeder for mechanical weeding can result in cost savings of up to 45% compared to the manual weeding method. The estimated break-even point (BEP), calculated based on time and area, indicates potential savings of 209.6 hours and 13.63 ha. The payback period, assessed on a time basis for the rotary power weeder, was determined to be 1.02 years. The total development cost for the self-propelled power weeder was recorded as 8050 Rupees. By adopting self-propelled power weeder, farmer saving 1856 ₹/ha directly over manual method.

Displacement and stress distribution pattern during complete mandibular arch distalization using buccal shelf bone screws - A three-dimensional finite element study.

To evaluate and compare the distribution of stress and displacement of teeth during mandibular arch distalization using buccal shelf screws. Three three-dimensional finite element models of mandibular arch were constructed with third molars extracted. Models 1, 2, and 3 were constructed on the basis of the lever arm heights of 0 mm, 3 mm, and 6 mm, respectively, between the lateral incisor and canine. A buccal shelf screw was placed at the area in the second molar region with the initial point of insertion being inter-dental between the first and second molars and 2 mm below the mucogingival junction. MBT pre-adjusted brackets (slot size 0.022 × 0.028") were placed over the clinical crown's center with a 0.019 × 0.025" stainless-steel archwire on three models. A retraction force of 300 g was applied with buccal shelf screws and a lever arm bilaterally using nickel-titanium closed coil springs. The displacement of each tooth was calculated on X, Y, and Z axes, and the von Mises stress distribution was visualized using color-coded scales using ANSYS 12.1 software. The maximum von Mises stress in the cortical and cancellous bones was observed in model 1. The maximum von Mises stress in the buccal shelf screw and the cortical bone decreased as the height of the lever arm increased. Applying orthodontic forces at the level of 6 mm lever arm height resulted in greater biomechanical bodily movement in distalization of the mandibular molars compared to when the orthodontic forces were applied at the level of 0 mm lever arm height. Displacement of the entire arch may be dictated by a direct relationship between the center of resistance of the whole arch and the line of action generated between the buccal shelf screw and force application points at the archwire, which makes the total arch movement highly predictable.

Numerical investigation on the flow resistance characteristics of balance block in the rotary compressor

With the increasing demand for energy saving, the air conditioner with variable frequency becomes the mainstream of the market, which leads to a high requirement for the dynamic balance characteristics of the rotary compressor at variable speed conditions. The balance block is the key part in the eccentric shaft system to balance the rotational inertia force brought by the eccentric wheel, and its flow resistance during rotating process has a large impact on the power consumption. In this paper, CFD simulations will be carried out to study the flow characteristics of several different compressor balance blocks and the results can provide reference for the optimization design of the balance block in the rotary compressor.

Multiple Shells Driven by Disk Winds: ALMA Observations in the HH 30 Outflow

We present archival Atacama Large Millimeter/submillimeter Array band 6 observations of the 13CO (J = 2–1) and 12CO (J = 2–1) molecular line emission of the protostellar system associated with HH 30. The 13CO molecular line shows the accretion disk while the molecular outflow is traced by the emission of the 12CO molecular line. We estimated a dynamical mass for the central object of 0.45 ± 0.14 M ⊙, and a mass for the molecular outflow of (1.83 ± 0.19) × 10−4 M ⊙. The molecular outflow presents an internal cavity as well as multiple outflowing shell structures. We distinguish three different shells with constant expansion (∼4–6 km s−1) and possible rotation signatures (≤0.5 km s−1). We find that the shells can be explained by magnetocentrifugal disk winds with launching radii R launch ≲ 4 au and a small magnetic lever arm λ ∼ 1.6–1.9. The multiple shell structure may be the result of episodic ejections of the material from the accretion disk associated with three different epochs with dynamical ages of 497 ± 15 yr, 310 ± 9 yr, and 262 ± 11 yr for the first, second, and third shells, respectively. The outermost shell was ejected 187 ± 17 yr before the middle shell, while the middle shell was launched 48 ± 14 yr before the innermost shell. Our estimations of the linear and angular momentum rates of the outflow as well as the accretion luminosity are consistent with the values expected if the outflow of HH 30 is produced by a wide-angle disk wind.

H i, FRB, What’s Your z: The First FRB Host Galaxy Redshift from Radio Observations

Identification and follow-up observations of the host galaxies of fast radio bursts (FRBs) not only help us understand the environments in which the FRB progenitors reside, but also provide a unique way of probing the cosmological parameters using the dispersion measures (DMs) of FRBs and distances to their origin. A fundamental requirement is an accurate distance measurement to the FRB host galaxy, but for some sources viewed through the Galactic plane, optical/near-infrared spectroscopic redshifts are extremely difficult to obtain due to dust extinction. Here we report the first radio-based spectroscopic redshift measurement for an FRB host galaxy, through detection of its neutral hydrogen (H i) 21 cm emission using MeerKAT observations. We obtain an H i–based redshift of z = 0.0357 ± 0.0001 for the host galaxy of FRB 20230718A, an apparently nonrepeating FRB detected in the Commensal Real-time ASKAP Fast Transients survey and localized at a Galactic latitude of –0.°367. Our observations also reveal that the FRB host galaxy is interacting with a nearby companion, which is evident from the detection of an H i bridge connecting the two galaxies. A subsequent optical spectroscopic observation confirmed an FRB host galaxy redshift of 0.0359 ± 0.0004. This result demonstrates the value of H i to obtain redshifts of FRBs at low Galactic latitudes and redshifts. Such nearby FRBs whose DMs are dominated by the Milky Way can be used to characterize these components and thus better calibrate the remaining cosmological contribution to dispersion for more distant FRBs that provide a strong lever arm to examine the Macquart relation between cosmological DM and redshift.

Combined vertical and external rotational force in plantarflexion position produces posterior pilon fracture: A preliminary cadaveric study

BackgroundPosterior pilon fracture is speculated to occur by a combination of rotation and axial load, which makes it different from rotational posterior malleolar fracture or pilon fracture, but is not validated in vitro. The aim of the current study is to investigate the injury mechanisms of posterior pilon fracture on cadaveric specimens. MethodsEighteen cadaveric specimens were mounted to a loading device to undergo solitary vertical loading, solitary external rotational loading, and combined vertical and external rotational loading until failure, in initial position of plantarflexion with or without varus. The fracture characteristics were documented for each specimen. ResultsVertical loading force combined with external rotation force diversified the fracture types resulting in pilon fracture, tibial spiral fracture, rotational malleolar fracture, talar fracture or calcaneal fracture. Vertical violence combined with external rotational loading in position of 45° of plantarflexion and 0° of varus produced posterior pilon fracture in specimens No. 13 and 14. ConclusionCombination of vertical and external rotational force in plantarflexion position on cadaveric specimens produce posterior pilon fracture.

Insufficient stem antetorsion and lower cup abduction is a combined risk factor for posterior hip dislocation in patients undergoing THA for femoral neck fractures: a retrospective analysis

BackgroundThe role of acetabular and femoral component positions with respect to the risk of post-operative instability and dislocation remains debated. In this study, we aimed to identify potential risk factors for early dislocation following primary total hip arthroplasty (THA) for displaced intracapsular femoral neck fractures (FNF) using radiological measurements.MethodsWe retrospectively analyzed data for patients who underwent cementless primary THA for FNF using a posterolateral approach between January 2018 and December 2021. Follow-up duration, age, sex, affected side, and mean time from THA to dislocation were recorded. Leg-length inequality, abductor lever arm, vertical and horizontal femoral offsets, vertical and horizontal hip centers of rotation, abduction, anteversion of the acetabulum and femoral prosthesis, and combined anteversion were measured.ResultsThe study sample included 17 men and 34 women, with 21 and 30 patients undergoing left- and right-hip operations, respectively. The mean patient age was 70.18 ± 7.64 years, and the mean follow-up duration was 27.73 ± 13.52 months. The mean time between THA and dislocation was 1.58 ± 0.79 months. Seven patients (13.73%) sustained posterior dislocation of the hip. The abduction angle (36.05 ± 6.82° vs. 45.68 ± 8.78°) (p = 0.008) and anteversion of the femoral prosthesis (8.26 ± 4.47° vs. 19.47 ± 9.01°) (p = 0.002) were significantly lower in the dislocation group than in the control group. There were no significant differences in other parameters.ConclusionsInsufficient stem antetorsion combined with lower abduction angle of the acetabular component were associated with a high risk of dislocation, especially in patients with deep flexion or internal rotation of the flexed hip joint and knees, or in patients with a stiff spine or anterior pelvic tilt, impingement may then occur in the neck of the prosthesis and cup component, ultimately resulting in posterior dislocation. These findings could remind surgeons to avoid simultaneous occurrence of both in THA surgery. These results provide new insight into risk factors for hip dislocation in patients undergoing primary THA for FNF and may aid in reducing the risk of instability and dislocation.Level of evidenceProspective comparative study Level II.

Subtle Syndesmotic Instability.

Latent or subtle syndesmotic instability is defined as an injury to the syndesmosis which is not apparent on static radiographs of the ankle. Syndesmotic injuries have also been referred to as high ankle sprains. Injury to the syndesmosis typically occurs with collision sports and often involves an external rotation force to the ankle. Diagnosis can be delayed because of negative initial imaging studies. Physical examination tests including the external rotation test, proximal squeeze test, and fibular shuck test can assist in the diagnosis. Advanced imaging modalities such as MRI and weight-bearing CT have been studied and can provide prognostic indications for management, although arthroscopic stress evaluation remains the benchmark for diagnosis. Both surgical and nonsurgical management techniques have been described, which can assist patients in returning to their preinjury level of function.

Improving the Industrial Efficiency of Recycling Aluminum Alloy Chips Using Friction Stir Extrusion: Thin Wires Production Process

Friction stir extrusion is one of the most promising solid-state chip recycling techniques because of its relative simplicity and high efficiency. One of the most straightforward applications for the process is the production of recycled wires to be utilized as filler material in welding or welding-based additive manufacturing processes, in order to create an industrial symbiosis link, fostering a circular economy and enhancing the technology readiness level of the process. The scalability of the process to the thin wires needed for such applications has not been investigated so far. In this paper, an experimental and numerical analysis was developed. A dedicated numerical model was first validated and then used to design the tool geometry. The effect of tool rotation and tool force on both “standard” mechanical properties, as Ultimate Tensile Strength and microhardness, and specific properties for the envisaged application, as the wrapping around reels with different radii, was investigated. The numerical model results were used to explain the influence of the process parameters on the material flow as well as on the distribution of the primary field variables, namely temperature, strain, and strain rate. Finally, the energy demand was measured, and the specific energy consumption (SEC) was evaluated. It was found that a conical shoulder surface favors the conditions for effective solid bonding. Low values of the extrusion force have detrimental effects on the wires properties as they result either in insufficient strain, or hot cracking defects. High values of extrusion force results in lower SEC, unlocking the potential of the process as symbiotic link enabler.

Blade shape influence on aerodynamic efficiency of a Magnus wind turbine using particle image velocimetry

Magnus wind turbine uses rotating cylinders instead of conventional horizontal axis blades. These cylinders rotate around their own axes and create a rotational force according to Magnus effect. Although this kind of wind turbines have many benefits over conventional axis blades and can exhibit different blade characteristics, it is not clearly demonstrated which the most efficient blade geometry is. This paper focuses on assess the influence of the blade shape on the Mangus force, using particle image velocimetry.

A Low-Cost and High-Precision Underwater Integrated Navigation System

The traditional underwater integrated navigation system is based on an optical fiber gyroscope and Doppler Velocity Log, which is high-precision but also expensive, heavy, bulky and difficult to adapt to the development requirements of AUV swarm, intelligence and miniaturization. This paper proposes a low-cost, light-weight, small-volume and low-computation underwater integrated navigation system based on MEMS IMU/DVL/USBL. First, according to the motion formula of AUV, a five-dimensional state equation of the system was established, whose dimension was far less than that of the traditional. Second, the main source of error was considered. As the velocity observation value of the system, the velocity measured by DVL eliminated the scale error and lever arm error. As the position observation value of the system, the position measured by USBL eliminated the lever arm error. Third, to solve the issue of inconsistent observation frequencies between DVL and USBL, a sequential filter was proposed to update the extended Kalman filter. Finally, through selecting the sensor equipment and conducting two lake experiments with total voyages of 5.02 km and 3.2 km, respectively, the correctness and practicality of the system were confirmed by the results. By comparing the output of the integrated navigation system and the data of RTK GPS, the average position error was 4.12 m, the maximum position error was 8.53 m, the average velocity error was 0.027 m/s and the average yaw error was 1.41°, whose precision is as high as that of an optical fiber gyroscope and Doppler Velocity Log integrated navigation system, but the price is less than half of that. The experimental results show that the proposed underwater integrated navigation system could realize the high-precision and long-term navigation of AUV in the designated area, which had great potential for both military and civilian applications.