Levels Of Total Phenolic Compounds Research Articles

Proximate Composition and Antioxidant Activity of Young Flattened Rice (Khao-Mao)

Khao-Mao producer group in Thailand’s Nakhon Phanom province’s Phon Sawan district, Na Hua Bo sub-district is a community that has produced Khao-Mao according to conventional wisdom, for a very long time. Khao-Mao has an emerald, green color, fragrant, delicate texture, and delicious taste, however the essential nutritional facts are not provided. Consequently, this study’s goal was to evaluate nutritional values by proximate analysis. All 6 types of young flattened rice-: Laos Wan, Kor Khor 6(RD6), Ai Lueng, San Pa Tong, Yee Pun, and Hom Nang Nuan have phenolic compounds by Folin-Ciocalteu method and antioxidant properties by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. According to the findings, rice's primary constituents are carbohydrates, which range from 69.76 to 73.07 %, protein, which ranges from 4.79 to 6.11 %, crude fat, which ranges from 1.68 to 2.22 %, fiber, which ranges from 0.00 to 0.03 % moisture, which ranges from 17.96 to 21.31 %, and ash, which ranges from 1.13 to 1.70 %. The San Pa Tong Khao-Mao has the highest 6.11% of protein content. Ai Lueng Khao-Mao has the greatest levels of total phenolic compounds, 0.79 mg/g dry weight. The DPPH radical scavenging assay test was used to measure the antioxidant activity. Their half-maximal inhibitory concentration (IC50) value falls between 1.18 and 2.00 mg/mL. The Hom Nang Nuan Khao-Mao has the lowest 1.18 mg/mL of IC50. According to the study’s findings, this community’s Khao-Mao has good nutritional and antioxidant properties. HIGHLIGHTS Khao-Mao has high proximate composition and high energy value Khao-Mao has relatively high quantities of protein and phenolic compounds Khao-Mao is a dish with a high-level antioxidant activity GRAPHICAL ABSTRACT

Calcium Nutrition in Fig Orchards Enhance Fruit Quality at Harvest and Storage

Due to the high perishability of figs, calcium stands out as an important nutrient for orchard management. This study aims to study the pre-harvest applications of calcium chloride in fig orchards. The fig trees were sprayed with 0, 0.5, 1.0, 1.5 and 2.0% CaCl2, and fruits were thereafter stored for 0, 7, 14 and 21 d. The variables analysed were the concentration of calcium in leaves and fruits, weight loss, fruit decay, pectinolytic enzyme activity, and physical and chemical attributes of the fruits; CaCl2 sprays enhanced fruit Ca2+ concentration in leaves (14.03%) and fruits (29.3%) and were effective in reducing polygalacturonase and pectin methylesterase activity, weight loss and fruit decay. Pre-treatment with 1% CaCl2 provided fruits with larger diameters, greater firmness, and higher levels of total phenolic compounds in both fruit peel and pulp. The pre-harvest application at 1% CaCl2 showed to be a promising technique in producing high quality fruits and extended storage by approximately 7 to 10 days. These findings may be useful in planning new cultural practices for fig orchards that produce high-quality fruit with desirable characteristics for growers and consumers.

Ispitivanje nutritivnog potencijala različitih genotipova lanika - mineralni sastav i bioaktivna jedinjenja

Camelina sativa is a member of the Brassicaceae family and is characterized as an annual oilseed plant with a short growth cycle. Its seeds offer rich nutritional value comparable with other feed and food sources due to the presence of high-quality oils, protein, essential fatty acids, and bioactive compounds. Our study investigated mineral profile and bioactive compound contents among two different camelina genotypes. The evaluated camelina genotypes NS Zlatka and NS Slatka were developed in Serbia. The bioactive compound investigation included total phenolic and flavonoid contents, chlorophyll a and b, and total carotenoid contents. Furthermore, antioxidant activity was assessed by measuring the DPPH-scavenging capacity of camelina seed extracts. The results demonstrate differences in mineral content between the two genotypes, with variations in sodium, potassium, calcium, magnesium, zinc, iron, manganese, copper, and phosphorus levels. Both genotypes exhibit notable mineral profiles, with particularly high levels of potassium (1007.76-1047.74 mg/100 g) and magnesium (224.09-227.45 mg/100 g), which makes them potentially valuable for both animal feed and human nutrition. Furthermore, analysis reveals substantial levels of total phenolic (8.13-8.16 mg GAE/g DM) and flavonoid (5.91-6.41 mg QE/g DM) compounds in both genotypes, indicating their suitability for applications in the food and pharmaceutical industries. Chlorophyll content analysis demonstrates significant differences between the two genotypes, particularly in chlorophyll a and chlorophyll b levels. These variations suggest differences in photosynthetic capacity and seed maturity. Total carotenoid content remains consistent between the genotypes at levels from 16.43 to 17.91 µg b-CE/g DM, implying similar antioxidant protection mechanisms. Overall, this study provides valuable insights into the biochemical properties of two camelina genotypes, indicating their potential applications in agriculture and human nutrition.

Hidroméis com méis brasileiros de diferentes origens botânicas

Abstract The objective of this work was to evaluate the quality of meads prepared with multifloral honey, unifloral orange blossom honey, and a mixture of both. To prepare the meads, multifloral honey and orange blossom honey from Southern and Southeastern Brazil, respectively, were used. The physicochemical properties, total phenolic content, and antioxidant capacity of the meads were determined. The phenolic compounds were identified by mass spectrometry, and sensory tests were carried out. Multifloral honey showed higher levels of ash content, reducing sugars, and total phenolic compounds than orange blossom honey. The multifloral and mixed meads presented the highest levels of total phenolics, total polyphenol index, and antioxidant capacity. The orange blossom mead showed the highest scores in the sensory analysis for color, aroma, flavor, and global acceptance. The phenolic compounds identified in the meads were the chlorogenic, protocatechuic, syringic, and p-coumaric acids, as well as naringenin and quercetin. The physicochemical, functional, and sensory characteristics of the meads are affected by the honey used in their preparation.

Pre-Breeding Genetic Diversity Assessment of Tomato (Solanum lycopersicum L.) Cultivars Based on Molecular, Morphological and Physicochemical Parameters

Appropriate knowledge of the parental cultivars is a pre-requisite for a successful breeding program. This study characterized fruit yield, quality attributes, and molecular variations of ten tomato cultivars during three consecutive generations under greenhouse conditions. Peto 86, Castle Rock, and Red Star cultivars showed the highest fruit yield (kg/plant), total phenolic compounds (TPC), and sap acidity. Principal component analysis categorized the evaluated fruit yield into three groups based on their quality attributes. A robust positive correlation appeared among traits inside each group. A positive correlation was likewise noticed between the first and the second groups. However, a negative correlation was detected between the first, the second and the third group. Molecular profiling, using seven inter-simple sequence repeat (ISSR) primers, produced 60 loci, including 49 polymorphic loci. The molecular analysis also pinpointed the highest genetic similarity (0.92) between P73 and Moneymaker, while the lowest genetic similarity (0.46) was observed between Castle Rock and Moneymaker. The cultivars P73 and Moneymaker showed the lowest genetic distance (2.24), while the highest genetic distance (5.92) was observed between Super Marmand and Peto86, on the one hand, and between Castle Rock and Moneymaker, on the other hand. The chemical analysis of fruit sap indicated the highest levels of TPC, total flavonoids, anthocyanin, ascorbic acid and total soluble solids in Peto 86 and Castle Rock cultivars. Phylogeny analysis of tomato cultivars based on morphological and molecular attributes indicated four distinct clades. Peto 86, Castle Rock, and Red star cultivars can be recommended for the tomato hybridization breeding programs in the future, with other tomato cultivars as potentially high-yielding parents.

Effects of Oak Leaf Extract, Biofertilizer, and Soil Containing Oak Leaf Powder on Tomato Growth and Biochemical Characteristics under Water Stress Conditions

Drought stress is one of the most significant abiotic stresses on the sustainability of global agriculture. The finding of natural resources is essential for decreasing the need for artificial fertilizers and boosting plant growth and yield under water stress conditions. This study used a factorial experimental design to investigate the effects of oak leaf extract, biofertilizer, and soil containing oak leaf powder on the growth and biochemical parameters of four tomato genotypes under water stress throughout the pre-flowering and pre-fruiting stages of plant development. The experiment had two components. The first component represented the genotypes (two sensitive and two tolerant), while the second component represented the treatment group, which included irrigated plants (SW), untreated and stressed plants (SS), treated plants with oak leaf powder and stressed (SOS), treated plants with oak leaf powder and oak leaf extract and stressed (SOES), and treated plants with oak leaf powder and biofertilizers and stressed (SOBS). When compared with irrigated or control plants, drought stress under the treatments of SS, SOS, SOES, and SOBS conditions at two stages and their combination significantly lowered shoot length (12.95%), total fruit weight per plant (33.97%), relative water content (14.05%), and total chlorophyll content (26.30%). The reduction values for shoot length (17.58%), shoot fresh weight (22.08%), and total fruit weight per plant (42.61%) were significantly larger in two sensitive genotypes compared with tolerant genotypes, which recorded decreasing percentages of 8.36, 8.88, and 25.32% for shoot length, shoot fresh weight, and total fruit weight per plant, respectively. Root fresh weight and root dry weight of genotypes treated with SS, SOS, SOES, and SOBS, on the other hand, increased in comparison with control plants. Tomato fruits from stressed plants treated with SS, SOS, SOES, and SOBS had considerably higher levels of titratable acidity, ascorbic acid, and total phenolic compounds than irrigated plants during all stress stages. Under water stress conditions, the addition of oak leaf powder to soil, oak leaf extract, and biofertilizer improved the biochemical content of leaves in all genotypes. Furthermore, leaf lipid peroxidation was lower in plants treated with SOES and SOBS, and lower in the two tolerant genotypes than in the two susceptible genotypes. In conclusion, the application of SOS, SOES, and SOBS demonstrated a slight decrease in some morpho-physiological and fruit physicochemical traits compared with SS treatment. However, the application of oak leaf powder and oak leaf extract can be described as novel agricultural practices because they are low-cost, easy to use, time-consuming, and can meet the growing demands of the agricultural sector by providing environmentally sustainable techniques for enhancing plant resistance to abiotic stress. The usage of the combination of leaf crude extract, oak leaf powder, and arbuscular mycorrhizal fungus should be investigated further under stress conditions.

Physiological and biochemical assay of drought stress responses in eggplant (Solanum melongena L.) inoculated with commercial inoculant of Azotobacter chroococum and Azotobacter vinelandii

• Decreased RWC, g s values of plants under drought stress, and especially K, Ca and Mg accumulations and protein contents in the root increased significantly with rhizobacteria inoculation. • Higher levels of total phenolic compounds and proline were accumulated in inoculated plants compared to uninoculated plants. • Higher antioxidant enzymatic activities were observed in inoculated stressed plants, and MDA was reduced. • Results were found to be important in that the inoculant with Azotobacter chroococum and Azotobacter vinelandii has the potential to diminish the negative effects of drought stress in eggplant . The aim of this study is to examine the effects of commercial rhizobacteria inoculant on eggplant plants grown under drought stress conditions. Commercial inoculant containing Azotobacter chroococum and Azotobacter vinelandii rhizobacteria species was applied to eggplant plants by root inoculation and the plants were exposed to different levels of drought stress (moderate drought stress-MS and severe drought stress-SS). To determine the growth-promoting ability of inoculation with rhizobacteria, changes in plant morphology (shoot-root fresh and dry weights, shoot length and diameter) and physiology (relative water content-RWC, stomatal conductivity-g s , K, Ca, Mg and Na accumulations in shoot and root, photosynthetic pigment contents) were investigated. To determine the impacts of the inoculant on the potential of increasing the drought tolerance of eggplant, besides the enzyme activities of superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GR), non-enzymatic antioxidant activities such as antochiyanin, total phenolic substance, proline were investigated. In addition, H 2 O 2 and malondialdehyde (MDA) contents were analyzed to resolve whether drought stress causes oxidative damage in eggplant. The increase in the severity of drought caused a decrease in plant growth and shoot-root fresh and dry weights. Nevertheless, these adverse effects of drought stress were alleviated by inoculation. Decreased RWC, g s values of plants under drought stress, and especially K, Ca and Mg accumulations and protein contents in the root increased significantly with inoculation. On the other hand, Chlorophyll (Chl) (Chl a , Chl b , Chl a + b ) and carotenoid contents were significantly increased in leaves under uninoculated MS and SS. Inoculation with rhizobacteria reduced the increase in photosynthetic pigment contents. Depending on the severity of stress, higher levels of total phenolic compounds and proline were accumulated in inoculated plants compared to uninoculated plants. However, higher SOD, CAT, and GR enzymatic activities were observed in inoculated stressed plants, and membrane lipid peroxidation was reduced. These results were found to be important in that the commercial bacterial inoculant has the potential to diminish the negative effects of drought stress in eggplant and supports the stress tolerance of the plant by mitigating the drought-related oxidative damage.

Postharvest quality of apples from ‘Maxi Gala’ trees grafted on different rootstocks

This study analyzed the effect of rootstocks G.213, M.9 and Marubakaido with M.9 interstem of 30 and 20 cm (MB/M.9-30cm and MB/M.9-20cm) on postharvest quality, mineral composition and functional properties of 'Maxi Gala' apples. The evaluations was performed using fruits from a commercial orchard located in Vacaria, RS, Brazil. Fruits were stored for 135 and 147 days in the 2014/2015 and 2015/2016 seasons, respectively, and analyzed one day after harvest and after cold storage (1.0±0.2 °C) followed by seven days in ambient condition. The mineral composition of fruits showed small and inconsistent differences between rootstocks. The MB/M.9-20cm provided higher total antioxidant activity and levels of total phenolic compounds in fruit peel than MB/M.9-30cm. For 2014/2015 season, soluble solids content at harvest was higher in fruits from trees with G.213 rootstock, not differing only from MB/M.9-30cm. In 2015/2016 season, G.213 and M.9 provided higher soluble solids content after storage compared to MB/M.9-30 cm and MB/M.9-20cm. G.213 rootstock anticipated fruit maturity compared to M.9 in 2014/2015 season. Other quality attributes of ‘Maxi Gala’ apples, at harvest and after cold storage, were similar between rootstocks G.213, M.9 and Maruba with M.9 interstem of 30 and 20cm.

Consumption of underutilised grain legumes and the prevention of type II diabetes and cardiometabolic diseases: Evidence from field investigation and physicochemical analyses

This study was carried out to correlate grain legume consumption to the prevention of type II diabetes and some cardiometabolic diseases (CMD) (high blood pressure, obesity, and stroke). An exploratory survey was carried out among the population (n = 468) of the Diamaré division (Far-north, Cameroon). In addition, nutritional properties of selected grain legumes (GLs) were determined. Results showed generally that consumption of soybean, Bambara bean, and cowpea was linked to the prevention of metabolic diseases within the studied population. The nutritional properties of these GLs were also noteworthy. Remarkable protein content was noted in soybean's whole flour (WF) (38.133±0.366 g/100g DM), followed by the underutilised cultivar BVB of Bambara bean (26.434±0.366 g/100g DM). The cowpea seed has the highest total carbohydrate and vitamin C content of 59.294±0.129 g/100g DM and 327.715±0.336 µg EAA/100g DM, respectively, followed by the underutilised cultivar BVB with values of 57.696±0.097 g/100g DM and 261.951±0.376 µg EAA/100g DM. Dietary fibre content of Bambara bean seeds (18 - 28%) was higher than those of the other seeds, and probably plays important role in the prevention ability of diabetes and CMD of Bambara bean. Soybean's WF and those of the three underutilised Bambara bean cultivars contained the highest levels of total phenolic compounds and flavonoids, i.e., 206.089±0.087 mg GAE/100 DM, 143.868±0.299 mg QE/100g DM for soybean, respectively. The highest free radical scavenging activity (DPPH) was noted with the polyphenolic compounds’ extracts from soybean's WF, i.e., 21.193±0.049 µg TE/100g DM, followed by those from the underutilised BVB cultivar with a value of 16.594±0.160 µg TE/100g DM. The highest ferric reducing antioxidant power (FRAP) was obtained with soybean (34.481±0.029 µg TE/100g DM), followed by the cultivar GHB (32.036±0.076 µg TE/100g DM). The significant levels of protein, phenolic compounds, and dietary fibre in these seeds confirmed these correlations and their potential therapeutic effects and nutritional properties. Given the richness of the grain legumes in proteins and other bioactive compounds, raw materials and/or protein hydrolysates (peptides) will surely be a promising raw material for the pharmaceutical industries in the fight and/or prevention of metabolic diseases, in the fight against food insecurity, in the prevention and/or reversal of neurodegenerative diseases, and the formulation of a new functional ingredient.

Synergistic Action of Multiple Enzymes Resulting in Efficient Hydrolysis of Banana Bracts and Products with Improved Antioxidant Properties

This study investigated the effect of enzymatic hydrolysis of banana bracts from different varieties (Maçã, Nanica and Prata) using pectinase, protease and cellulase (singly or in combinations) on their antioxidant properties. The results showed that the antioxidant properties and total phenolic compounds (TPC) of extracts increased after the enzymatic treatment with a clear synergistic effect between the different enzymes. The ternary mixture of pectinase, protease and cellulase resulted in increases of 458% and 678% in TPC content for extracts obtained from Maçã and Nanica varieties and up to 65% in antioxidant properties of those produced from Prata variety compared to the non-hydrolyzed samples. In general, the extracts obtained from the Prata variety showed the highest levels of TPC, as well as antioxidant activity, as follows: 14.70 mg GAE g−1 for TPC, 82.57 µmol TE g−1 for ABTS, 22.26 µmol TE g−1 for DPPH and 47.09 µmol TE g−1 for FRAP. Phenolic compounds identified by HPLC in extracts included ρ-coumaric, ferulic, sinapic and vanillic acids and the flavonoid rutin. This study reported for the first time the enzymatic treatment applied to banana bracts as a promising method to release antioxidant compounds, offering a new opportunity to explore these residues as a source of molecules with high added value through an environmentally friendly and safe process.

Comparative Analysis of Antioxidant Compounds and Antioxidative Properties of Thai Indigenous Rice: Effects of Rice Variety and Processing Condition.

Indigenous southern Thai non-glutinous rice varieties Kaab Dum, Khai Mod Rin, Yar Ko, Yoom Noon, and Look Lai made under four different processing conditions, white rice, brown rice, germinated brown rice, and rice grass, were assessed for antioxidant components and in vitro antioxidative activities. According to the findings, rice’s antioxidant components and antioxidant activity were considerably impacted by both variety and processing. High levels of total extractable phenolic compounds (164–314 mg gallic acid equivalent (GAE)/kg, dry weight (dw)) and carotenoid (0.92–8.65 mg/100 g, dw) were found in all rice varieties, especially in rice grass and germinated brown rice, indicating that milling to generate white rice had an adverse effect on those components. Additionally, after germination, a higher γ-oryzanol concentration (9–14 mg/100 g, dw) was found. All rice varieties had higher ascorbic acid, phenolic compound, and carotenoid contents after sprouting. Overall, Yoom Noon rice grass had the highest total extractable phenolic content (p < 0.05). The rice grass from Yoom Noon/Look Lai/Kaab Dum had the highest ascorbic acid content (p < 0.05). The total carotenoid concentration of Look Lai rice grass was the highest, and Yoom Noon’s germinated brown rice had the highest γ-oryzanol content (p < 0.05). All rice varieties’ aqueous extracts had remarkable ABTS free radical scavenging activity, with Khai Mod Rin reaching the highest maximum value of 42.56 mmol Trolox equivalent/kg dw. Other antioxidant mechanisms, however, were quite low. Compared to germinated brown rice, brown rice, and white rice, rice grass often tended to have stronger antioxidant activity. Yar Ko rice grass was found to have the highest DPPH free radical scavenging activity (3.8 mmol Trolox equivalent/kg dw) and ferric reducing antioxidant power (FRAP) (4.6 mmol Trolox equivalent/kg dw) (p < 0.05). Khai Mod Rice grass had the most pronounced metal chelation activity (1.14 mmol EDTA equivalent/kg dw) (p < 0.05). The rice variety and processing conditions, therefore, influenced the antioxidant compounds and antioxidative properties of Thai indigenous rice. The results can be used as a guide to select the optimal rice variety and primary processing in order to satisfy the needs of farmers who want to produce rice as a functional ingredient and to promote the consumption of indigenous rice by health-conscious consumers.

Evaluation of the effect of substrates and types of wheat flour on microbiological characteristics, pH values, levels of total phenolic compounds, antioxidant capacity and fermentative capacity of sourdough

Sourdough is a process that is expanding nowadays. However, the microorganisms present produce metabolites that can cause changes in the breads. Thus, the objective of this work was to evaluate the effects of substrates and types of wheat flour on microbiological characteristics, pH values, total phenolic compounds, antioxidant capacity and fermentative capacity of different types of sourdough, comparing them with dry yeast. In this way, six sourdough were elaborated, diversifying the type of wheat flour (whole or white) and the substrate (beer wort, pineapple juice or natural yogurt), being evaluated in the microbiological aspects (yeast count, bacteria under anaerobic conditions and under aerobic conditions), physicochemical (pH, phenolic compounds and antioxidant capacity (β-carotene/linoleic acid, ABTS and DPPH)) and fermentative capacity (only in sourdough). In view of the results obtained, it was observed that both white wheat flour and whole wheat flour, with the substrates yogurt, pineapple juice and brewer's wort, showed differences in microbiological characteristics, in pH values, in the content of compounds total phenolics, antioxidant capacity and fermentative capacity compared to dry yeast, with whole wheat flour, pineapple or beer wort providing microbial diversity, as well as lowering the pH. WY (white wheat flour and yogurt), WP (white wheat flour and pineapple) and IW (whole wheat flour and beer wort) sourdough were more resistant to temperatures (25 °C, 30 °C e 35 °C).

The effects of different extraction solvents on the yield and antioxidant properties of Nigella sativa oil from Saudi Arabia

Nigella sativa L. is a fragrant plant that belongs to the Ranunculaceae family. It is commonly used as a form of traditional medicine in Middle-Eastern countries. In this study, Nigella seed oil from Saudi Arabia was extracted using the cold press and Soxhlet methods to investigate the effects of solvents polarities on yield and antioxidant properties. The analysis revealed that the highest yield (40%) of oil was obtained with ethanol solvent. The aqueous methanol extract the highest level of total phenolic compounds (239 mg GAE/g). Moreover, the methanol extract showed the most elevated 2,2-Diphenyl-1-picrylhydrazyl radical rummaging assay, at 59%. The ferric reducing and antioxidant power activities of the oils extracted with ethanol and tetrahydrofuran were 13,199 and 13,326 mmol Fe2+/100 g dry weight, respectively. Thus, the findings show that a high level of natural antioxidants can be derived from Nigella sativa oil extracted by ethanol solvent.

Dual Response Optimization of Ultrasound-Assisted Oil Extraction from Red Fruit (Pandanus conoideus): Recovery and Total Phenolic Compounds

Red fruit oil (RFO) is a high-value oil that contains functional compounds, mainly phenolic compounds, providing antioxidant activity. Therefore, an optimal extraction method is essential to recover the RFO and phenolic compounds simultaneously. This research aimed to optimize the ultrasound-assisted extraction (UAE) for oil from red fruit using the Box-Behnken design combined with response surface methodology. The studied UAE factors, including sample-to-solvent ratio (1:3, 1:2, and 1:1 g mL−1), extraction temperature (60, 75, and 90 °C), and pulse duty-cycle (0.20, 0.50, and 0.80 s−1). Analysis of variance revealed that the three studied factors significantly influenced the recovered RFO, while the level of total phenolic compounds in the extracts was defined merely by extraction temperature (p &lt; 0.05). These significant factors were then included in the optimization models (R2 &gt; 0.99, lack-of-fit p &gt; 0.05). The proposed UAE setting by the multiresponse optimization was an extraction temperature of 67 °C, a pulse duty-cycle of 0.50 s−1, and a sample-to-solvent ratio of 1:2.5 g mL−1. Subsequently, the extraction kinetic was evaluated, confirming full recovery at 60 min of extraction time. The developed method was then applied to extract six red fruit clones. Mbarugum clones provided high RFO recovery (9.60%), with an uppermost total phenolic compound of (42.63 mg GAE g−1) among the six red fruit clones. Additionally, the resulting RFO showed eminent antioxidant activities, indicated by excellent values of IC50 DPPH (37.69 mg L−1), IC50 FIC (30.43 mg L−1), FRAP reducing power (63.55 mg AAEA g−1), and IC50 ABTS (93.88 mg L−1). In contrast with a wet rendering method, UAE enhanced the RFO recovery by 53.02%, resulting in a higher level of total phenolic compounds. Henceforth, the proposed UAE method is a promising technique to substitute conventional oil production in the food and pharmaceutical industries.

Screening coffee genotypes for brown eye spot resistance in Brazil.

Several researchers have attempted to develop coffee plants that are resistant to brown eye spot (BES); however, no coffee cultivars are resistant to the disease. In the present study, a blend of strains from Cercospora coffeicola was inoculated into 19 Brazilian commercial cultivars and 41 accessions from the Germplasm Collection of Minas Gerais to evaluate the genetic resistance ability within the population and select superior genotypes for the breeding program. After predicting the genotypic values of the estudied material, the evaluations number necessary for selecting genotypes with accuracy and efficiency was determined based on the data of severity to BES. The action of defense mechanisms plant was also investigated by assessing the levels of total soluble phenolic compounds and soluble lignin in contrasting genotypes for disease susceptibility. Based on the results, the accession MG 1207 Sumatra, had an intrinsic genetic capacity to maintain low levels of severity to BES. The genotype MG 1207 Sumatra can substantially contribute to the development of new cultivars, which may lead to the reduced use of pesticides. According to the accuracy and efficiency results obtained, four evaluations BES severity are sufficient to achieve accuracy, providing expressive genetic gains. Finally, the levels of lignin and phenolic compounds were not found to be associated with the resistance of coffee genotypes to BES.

Pumpkin landraces from southern Brazil as functional foods

Squash is a food that meets the requirements of a healthy diet and is highly appreciated by consumers for its mild flavour and high nutritional value. In southern Brazil, many farmers grow several pumpkin landraces that are widely used in food. The consumption of its fruits and derivatives benefits human health due to the bioactive compounds, minerals, and antioxidants in the fruit pulp, making pumpkin a functional food. The objective of this study was to characterize the bioactive compounds, antioxidant activity, and minerals in the pulp of fruits of pumpkin landraces (Cucurbita maxima) from southern Brazil. Phenolic compounds, carotenoids, antioxidant activity, and minerals were evaluated in 10 accessions of pumpkin landraces from the Cucurbitaceae Genebank of Embrapa Temperate Agriculture (Pelotas, RS, Brazil). In mature fruits, the seeds and peels were discarded, and opposite longitudinal slices of pulp were manually prepared for analysis. All analyses were performed in triplicate. The data obtained showed genetic variability in total phenolic compounds, carotenoids, antioxidant activity, and minerals. Accessions C49, C307, and C216 had high levels of total phenolic compounds and carotenoids and high antioxidant activity. Of these accessions, accession C49 had the highest antioxidant capacity (504.1 μg·g-1). C216 and C178 were high in copper and iron, and the latter was highest in potassium, calcium, and phosphorus. Thus, accessions C216 and C178 have great potential for exploitation by genetic improvement programs for the development of biofortified cultivars in efforts to promote consumer health.

Selected phenolics and antioxidant capacities: From Boğazkere (Vitis vinifera L.) grape to pomace and wine

In this research, the amounts of trans-resveratrol, (+)-catechin, (-)- epicatechin, and malvidin-3-glucoside which are among phenolic compounds as well as the total phenolic compound, total anthocyanin, and antioxidant capacity changes were examined in the Boğazkere (Vitis vinifera L.) grape, pomace before press, pomace after press and wine. In all parameters examined, a decrease was seen when proceeding from grape to wine, only (+)- catechin and (-)- epicatechin levels were detected to be higher in the prepress pomace. Total phenolic compound, total anthocyanin, and antioxidant capacity levels were found highest in grape samples, respectively, 8018 mg GAE/kg; 1606 mg/kg; 16.05 μmol troloxl/g with ABTS method; 7.75 μmol trolox/g with DPPH method; 4.78 μmol trolox/g with FRAP method. The highest amount of trans-resveratrol content was obtained from grape berry with 3.57 mg/kg, the highest (+)- catechin content was obtained from the pomace before press sample with 54.66 mg/kg, the highest amount of (-)- epicatechin was obtained from pomace before press sample 20.38 mg/kg and the highest amount of malvidin-3-glucoside amount was determined as 510 mg/kg in grape. According to the results of the research, it was understood that the pomace of the Boğazkere variety, which is one of the grape varieties mostly processed as wine in our country, has a high level of antioxidant capacity and phenolic content.

Do chemical and nutritional compounds change during the storage of Jabuticaba (Plinia cauliflora)?

Jabuticaba (Plinia cauliflora) is a native Brazilian Atlantic Rainforest fruit tree. Its fruits are purplish berries with a short shelf life, due to fermentative processes that begin shortly after harvest. Recently, commercial jabuticaba exploitation has intensified, justifying the application of postharvest technologies. In this context, this study aimed to evaluate the chemical characteristics and nutraceutical compounds of “ponhema” jabuticabas stored at room and cold temperatures. Chemical analyzes (soluble solids (SS), titratable acidity (TA), pH, soluble sugars, soluble pectins and pectinamethylesterase (PME) activity), and nutraceutical compounds (total anthocyanins (TA), yellow flavonoids (YF), total phenolic compounds (TFC)) were performed. A completely randomized experimental design was applied. Analyzes were performed every 2 days, at days 0, 2, 4 for the room temperature (25° C) assay and at days 0, 2, 4 and 6 for the cold temperature (13° C) experiment. Fruits stored under cold temperature presented lower acetaldehyde and ethanol contents, as well as high soluble sugar, total anthocyanin, and total phenolic compound levels. Fruits stored at room temperature displayed marked wilting and fermentation on the fifth day of storage, preventing their consumption after this period. Fruits presented a shelf-life gain of up to two days when stored at cold temperature, displaying better characteristic maintenance, such as soluble solids, titratable acidity, pH and soluble sugars, which were verified by the acetaldehyde and ethanol tests. Total anthocyanin and phenolic compound levels were higher in fruits stored at cold temperature

Optimization of Microwave-Assisted Extraction of Polyphenols from Lemon Myrtle: Comparison of Modern and Conventional Extraction Techniques Based on Bioactivity and Total Polyphenols in Dry Extracts

The aromatic herb lemon myrtle is a good source of polyphenols, with high antioxidant and antimicrobial capacity. In this study, the green extraction technique microwave-assisted extraction (MAE) was applied and the extraction parameters were optimized using response surface methodology (RSM) to maximize the extraction yield of phenolic compound and antioxidant properties. Then, it was compared with other popular novel and conventional extraction techniques including ultrasound-assisted extraction (UAE) and shaking water bath (SWB) to identify the most effective technique for extraction of phenolic compounds from lemon myrtle. The results showed that the MAE parameters including radiation time, power, and sample to solvent ratio had a significant influence on the extraction yield of phenolic compounds and antioxidant capacity. The optimal MAE conditions were radiation time of 6 min, microwave power of 630 W, and sample to solvent ratio of 6 g/100 mL. Under optimal conditions, MAE dry extract had similar levels of total phenolic compounds (406.67 ± 8.57 mg GAE/g DW), flavonoids (384.57 ± 2.74 mg CE/g DW), proanthocyanidins (336.54 ± 7.09 mg CE/g DW), antioxidant properties, and antibacterial properties against (Staphylococcus lugdunensis and Bacillus cereus) with the other two methods. However, MAE is eight-times quicker and requires six-times less solvent volume as compared to UAE and SWB. Therefore, MAE is recommended for the extraction of polyphenols from lemon myrtle leaf.

Sustainable fermentation approach for biogenic hydrogen productivity from delignified sugarcane bagasse

Improper lignocellulosic wastes management causes severe environmental pollution and health damage. Conversion of such wastes particularly sugarcane bagasse (SCB) onto bioenergy is a sustainable approach due to a continuous depletion of conventional biofuels. The delignification of SCB is necessary to proceed for bio-genic H2 productivity by anaerobic bacteria. The effect of autoclaving, pre-acidification/autoclaving and pre-alkalization/autoclaving of SCB on glucose recovery and subsequently H2 productivity by dark fermentation was comprehensively investigated. Pre-acidified SCB with 1% H2SO4 (v/v) provided H2 productivity of 8.5 ± 0.14 L/kg SCB and maximum H2 production rate (Rm) of 105.9 ± 8.3 mL/h. Those values were dropped to 2.7 ± 0.13 L/kg SCB and 58.3 ± 12.9 mL/h for fermentation of delignified SCB with 2% H2SO4. This was linked to high levels of total phenolic compounds (1775.3 ± 212 mg/L) in the feedstock. Better H2 productivity of 13.9 ± 0.58 L/kg SCB and Rm of 133.9 ± 3.6 mL/h was achieved from fermentation of pre-alkalized SCB with 1%KOH (v/v). 256.8 ± 9.8 U/100 mL of α-amylase, 165.7 ± 7.6 U/100 mL of xylanase, 232.8 ± 6.1 U/100 mL of CM-Cellulase, 176.5 ± 5.0 U/100 mL of polyglacturanase and 0.702 ± 0.013 mg M B. reduced/min. of hydrogenase enzyme was accounted for the batches supplied with delignified SCB by KOH. The Clostridium and Bacillus spp. was dominance and prevalence resulting a higher H2 productivity and yield. A novel strain of Archea and alpha proteobacterium were also identified and detected.