Levels Of Tannins Research Articles

Composição centesimal, minerais, taninos, fitatos e qualidade de cozimento de cultivares comerciais de feijão-caupi

ABSTRACT Cowpea is a socioeconomically important legume in the Northeast region of Brazil, and the most grown and consumed type of bean, representing the main source of low-cost vegetable protein for rural and urban populations in this region. The objective of this work was to characterize the proximate composition, minerals, tannins, phytic acid, and cooking quality of whole grains of cowpea from the commercial cultivars BRS Aracê, BRS Inhuma, and BRS Xiquexique. Grain samples of the cultivars were ground in a zirconium ball mill and the flour was used for analysis. The proximate composition was carried out according to the AOAC methodology. The minerals were determined by nitro-perchloric digestion and reading in a flame atomic absorption spectrophotometer. The anti-nutritional factors tannins and phytic acid were determined by extraction and reading in a spectrophotometer. Cooking quality was assessed using an electric pressure cooker and then a Mattson cooker. A completely randomized experimental design was used, with three treatments (cultivars) and three replications. The data were subjected to analysis of variance and the means were compared by the Tukey's test (p<0.05). The cowpea cultivars showed significant differences (p<0.05) for most characteristics evaluated and were similar in terms of ash, lipids, total dietary fiber, and most macrominerals. The results showed that grains of the cultivars BRS Aracê and BRS Xiquexique are excellent sources of dietary fiber, proteins, and minerals and present high cooking quality, while grains of the cultivar BRS Inhuma stood out regarding carbohydrates, soluble dietary fibers, total energetic value, and low levels of factors, anti-nutritional tannins and phytic acid.

Effect of enset (Ensete ventricosum) varieties and fermentation time on nutritional compositions, antinutritional factors, functional properties, and sensory acceptance of Bulla.

The effect of enset varieties and fermentation time on the nutritional composition, antinutritional content, functional properties, and sensory acceptance of bulla was assessed. Bulla samples were prepared from newly improved enset varieties yanbule, gewada, zereta, and messina and were fermented for 0, 15, and 30 days. The result revealed that bulla prepared from gewada had relatively better values of fat (0.3 g/100 g), fiber (1.04 g/100 g), carbohydrate (97.7 g/100 g), energy (394.2 Kcal), and Fe (2.54 mg/100 g). Yanbule had relatively higher ash content (1.05 g/100 g) and considerably higher Ca (317.9 mg/100 g) than bulla prepared from the other varieties. Mg (56.8 mg/100 g) and Zn (2.3 mg/100 g) were relatively higher in bulla prepared from Messina. A very low level of tannin was detected only for gewada, but high contents of phytate up to 112.5 mg/100 g were obtained. With respect to the functional properties of bulla fermented for 30 days, no significant differences were observed among the varieties except for water absorption capacity. In terms of sensory quality, bulla porridge prepared from yanbule had comparatively higher overall acceptability score (7.6).

Bioactive compounds contents of and antioxidant activity of Nectan-dra megapotamica (Lauraceae)

Nectandra megapotamica (Spreng.) Mez., Lauraceae known as cinnamon, is a species native to Brazil, widely used in folk medicine. The present project aimed to evaluate the antioxidant activity and determine the contents of phenols, flavonoids, tannins, and total alkaloids in extracts from the leaves and bark of a specimen of N. megapotamica that occurs in the southern region of the state of Mato Grosso do Sul. Crude ethanolic extracts obtained from leaves and bark were tested for antioxidant activity by DPPH and ABTS methods and dosage of phenols, flavonoids, tannins, and total alkaloids by spectrophotometry in the visible region. The extracts exhibited considerable antioxidant activity, particularly the leaf extract was the most active in both methods, yielding IC50 of 9.0 ± 0.18 and 14.29 ± 0.16 μg.mL-1, respectively. This extract showed higher levels of phenols, flavonoids, tannins, and total alkaloids. The results suggest that the antioxidant activity is related to the presence of phenolic compounds and alkaloids, known antioxidant compounds, quantified in this study.

Phytochemicals, Nutrient Levels and Antioxidants of Various Types of Sweet Potatoes (Ipomoea batatas L.)

This study aims to identify the phytochemical compounds, including flavonoids, tannins, phenols, beta-carotene, anthocyanins, and fiber, as well as the antioxidant capacity of various types of sweet potatoes. Method: The research followed a descriptive observational design with laboratory-based testing. The identification of phytochemical compounds utilized specific reagents. One Way ANOVA was employed to assess the differences in average levels of phytochemicals, nutrients, and antioxidant capacity (IC50) among the different types of sweet potatoes with normally distributed data. The determination of flavonoid, tannin, phenol, beta-carotene, and anthocyanin levels was carried out using UV/Vis Spectrophotometry at specific wavelengths. Crude fiber content was determined using the gravimetric method, while the antioxidant capacity was measured using the DPPH (2,2-Diphenyl-1-Picrylhydrazyl) method. Results: The findings indicated that all types of sweet potatoes contain alkaloids, while some lack steroids. Purple sweet potatoes showed the highest levels of flavonoids, tannins, and phenols at 627.27, 1727.27, and 1507.14 mg/100 g, significantly differing from other types of sweet potatoes (p = 0.00). Besides, purple sweet potatoes exhibited the highest anthocyanin content (18.35 mg/100 g) compared to other types, showing a significant difference (p = 0.00). Orange sweet potatoes exhibited the highest levels of beta-carotene and fiber, at 15.49 and 5.63 mg/100 g, respectively, although the difference was not statistically significant (p = 0.83). The antioxidant capacity (IC50) of purple, orange, white, and yellow sweet potatoes were 45.13, 266.59, 190.01, and 210.73 ppm, respectively. Conclusion: Different varieties of sweet potatoes exhibit variations in phytochemical composition and antioxidant capacity. Purple sweet potatoes are rich in flavonoids, tannins, phenols, and anthocyanins, with significant antioxidant potential. Orange sweet potatoes show high levels of beta-carotene and fiber but have comparable antioxidant activity to white and yellow varieties

Exploring the Synergistic Antimicrobial Potential of Ethanolic Leaf Extracts from Melia azedarach and Solanum incanum against Escherichia coli

Medicinal plants have been used for the maintenance of health for many years. Emerging cases of adverse drug reactions, high cost, and antimicrobial resistance using a single antimicrobial drug have heightened the need for research into plant sources of antimicrobial activity like Melia azedarach and Solanum incanum. This research investigated the synergistic antimicrobial activity exhibited by ethanolic leaf extracts obtained from Melia azedarach and Solanum incanum when tested against the pathogenic bacterium Escherichia coli. The study aimed to characterize the presence and composition of phytochemicals in the individual extracts, thereby elucidating their contribution to the observed antimicrobial effects. Additionally, minimum inhibitory concentration (MIC) assays and disc diffusion tests were performed to determine the optimal concentration required to inhibit the growth of Escherichia coli. Solanum incanum at a concentration of 2g/ml showed an average zone of inhibition of 6.92mm while Melia azedarach exhibited a zone of inhibition of 6.97 mm for Escherichia coli. The combined extracts showed a zone of inhibition of 8.34 mm for Escherichia coli. The minimum inhibitory concentration for Solanum incanum was 0.5g/ml while that of Melia azedarach was 1.4g/ml for Escherichia coli. Leaf extracts of both Melia azedarach and Solanum incanum had moderate to high levels of tannins, coumarins, flavonoids, and anthraquinones. Alkaloids and saponins were in low amounts in both extracts while steroidal glycosides were in moderate amounts in Melia azedarach but high in Solanum incanum. In conclusion, both plant extracts demonstrated significant antibacterial effects with both plants having a similar potency in terms of zone of inhibition diameter. The combined leaf extract of both plants had higher activity compared to individual plant extracts. We thus recommend that the combined extract can be used as an alternative modality for treating E. coli infections of mild severity without complications.

Antioxidant Activity and Phytochemical of Ocimum basilicum to Strengthen the Traditional Balinese Medicine System

The system of traditional Balinese medicine is known as Usada. One type of plant used in Usada is basil (Ocimum basilicum L). Antioxidant activity and phytochemical of ethanol extracts of two varieties of basil, Ocimum basilicum L (var.) Bali (OcB) and Ocimum basilicum L (var.) Lombok (OcL) was investigated. Total phenol was determined by folin-cioccalteu phenol reagent, total flavonoid by quarsetine reagent, tannin by Folin-Denis reagent, and antioxidant activity was determined using the 2,2-diphenyl-1-picrylhydrazyl radical scavenging method and is expressed as IC50. All of the parameters were measured by spectrophotometer. Phytochemicals were determined by Gas chromatography and mass spectrometry (GC-MS) Analysis. Morphological characters (stems, leaves, and flowers) are also observed. The results showed, in OcB obtained 92 compounds, 10 of which are important compounds that have an antioxidant effect, while in OcL 139 compound components were found, 15 of which are important compounds. Levels of phenols, flavonoids, tannins, as well as IC50 in OcB are; 3526.65 mg GAE/100g, 15841.6795 mg QE/100g, 2402.80 mg/100 g, and 24.9410 mg/L, respectively, while in OcL respectively are; 3297.71 mg GAE/100g, 13242.30 mg QE/100g, 850.71 mg/100, and 33.1105 mg/L. Conclusions, morphologically OcB and OcL showed no difference except leaf width and petiole length. The antioxidant activity of OcB is better, as well as higher levels of phenols, flavonoids, and tannins compared to OcL.

Facile extraction and prospective application of indigenous Cassia fistula tannin in sustainable leather manufacture

Vegetable tanning compounds have recently gathered attention as an environment-friendly, sustainable alternative to basic chromium sulfate in leather manufacturing. This study used different solvents such as water, methanol, and ethanol to extract tannins from Cassia fistula bark, a waste part of a tree. Ethanol proved the most effective solvent, yielding an extraction efficiency of 21.4 ± 0.61%. The bark extract was characterized with Fourier Transform Infrared Spectroscopy (FTIR), UV-Vis spectrophotometry, and Reverse Phase High Performance Liquid Chromatography (RP-HPLC). Gallic acid and rutin were discovered at high levels of tannins and polyphenolic compounds, including gallic acid (114.29 mg/100 g dry extract) and rutin (45.71 mg/100 g dry extract) whereas catechol and cinnamic were absent. The extracted bark exhibited a pH of 4.01 ± 0.04 and a moisture content of 4.89 ± 0.17%. Full vegetable, semi-chrome, and chrome re-tanned upper leathers were prepared using Cassia fistula bark extract to assess the tanning performance. Full vegetable leather displayed a reasonable shrinkage temperature of 85.77 ± 1.83 °C. All three types of upper leather met the United Nations Industrial Development Organization (UNIDO) standards of different parameters such as tensile, tear, and grain cracking strength, elongation, distention at grain crack, and flexing endurance. Field Emission Scanning Electron Microscope (FESEM) analysis revealed high fiber dispersion, indicating a higher cross-linking reaction within the leathers. Overall, the results suggested that Cassia fistula bark tannin may serve as a viable and renewable substitute for chrome tanning agents, providing a sustainable source of natural tanning material for leather manufacture.

Effects of Increasing Levels of Total Tannins on Intake, Digestibility, and Balance of Nitrogen, Water, and Energy in Hair Lambs.

This study aims to evaluate the effects of increasing tannin levels from Mimosa tenuiflora hay on the intake, digestibility, and balance of nitrogen (N), water, and energy in hair lambs. Thirty-two Santa Ines lambs, at an average age of 150 days and body weight of 26.75 ± 2.29 kg, were randomly assigned to four treatments in a completely randomized design. The treatments consisted of four diets: a control diet, tannin-free, and three diets with increasing levels of total tannin, 26.2, 52.4, and 78.6 g tannin/kg dry matter (DM). Including the total tannins in the lambs' diet led to a quadratic increase in the intake of nutrients, N-retention (g/day), water intake, water absorption and retention, energy intake, and energy excretion in feces and gases. However, the digestibility of crude protein, neutral and acid detergent fibers, and total carbohydrates decreased. It was observed that there is a correlation between the variable nutrient digestibility and N-ingested and the N-absorbed, N-urinary, and N-retained. However, the N-excreted in feces did not correlate with any of the variables studied. It is recommended to include 33 g/kg DM of total natural tannins from Mimosa tenuiflora hay in the diet of hair lambs, as it improves intake, energy balance, dietary N, and body water composition while reducing the excretion of N-urinary and gas emissions to the environment.

Genetic analysis of grain biochemical parameters and yield in pearl millet [Pennisetum glaucum (L.) R. Br.

Pearl millet is a potential crop to combat micronutrient deficiency through genetic biofortification of micronutrients in addition to protein, carbohydrates and oil. There is very limited information available regarding gene action for micronutrient content and other biochemical properties like carbohydrate, protein, oil and tannin content in pearl millet. ANOVA revealed significant differences between families and generations for all biochemical traits except protein content. Generation mean analysis through a six-parameter model exposed a prevalence of all kinds of genetic effects that govern Fe and Zn concentrations in grain in crosses 15611R × 42182-HP and 15695R × 15787R, respectively. Furthermore, fixable and heritable genetic variance like additive and additive × additive were found substantial for Fe content in most of the families. Zinc, tannin content and grain yield per plant were governed by heritable but non-fixable intra-allelic interactions in most of the crosses. Likewise, carbohydrate content showed significant additive gene action in crosses except cross 6055B × 15611R. The cross 6055B × 15611R provided the opportunity for developing a biofortified variety with a low tannin level as it exhibited negative heterosis for tannin content and positive heterosis for iron, zinc and carbohydrates content.

INVESTIGATION ON THE CONTENT OF PHENOLIC COMPOUNDS AND ANTIOXIDANT ACTIVITY OF INSTANT COFFEES FROM COFFEA ARABICA

Coffee is one of the most popular and consumed beverage worldwide both for the organoleptic characteristics and for the bioactive compounds’ content. Previous studies have shown that despite some risks it brings to health, it is useful in preventing cardiovascular diseases, liver diseases and hyperglycemia, and adding creams based on milk proteins helps to preserve its antioxidant activity after digestion. The content of total phenolics, flavonoids, tannins and the antioxidant activity were assessed in ten commercially soluble coffee samples. The results showed that the total phenolics content ranged from 1783.45 to 2061.30 mg gallic acid 100-1 g, the total flavonoids content ranged from 7637.55 to 13373.67 mg quercitin 100-1 g and condensed tannins level ranged from 1701.21 to 2176.57 mg catechin 100-1 g. The total antioxidant activity varied from 5513.84 to 7905.11 mg ascorbic acid 100-1 g. The differences found among the investigated samples containing 100% Coffea arabica might be due to the different bean processing. Despite these variations, instant coffees represent an additional source of bioactive compounds with antioxidant properties.

Metabolomic Characterization of Phoradendron brachystachyum Mistletoe and In-Silico and In-Vitro Investigation of Its Therapeutic Potential in Metabolic Disorders.

Plants of the Phoradendron genus have been traditionally used for their lipid- and glucose-lowering effects. However, the compounds responsible for these effects and the overall chemical profile of these plants have not been thoroughly investigated. We aimed to characterize the metabolome of leaves, stems, and aerial parts of the Phoradendron brachystachyum plant. We used mass spectrometry and colorimetric screening techniques (with various solvents) to identify and characterize the metabolites present. We also evaluated the antioxidant (FRAP, ORAC, TEAC, and DPPH assays) and inhibitory effects on pancreatic lipase and α-glucosidase enzymes of hydrophilic extracts. Furthermore, we compared the molecular fingerprints between the identified metabolites and FDA-approved drugs to gain insights into the metabolites that might be responsible for the observed effects on enzymes. Our findings revealed the presence of 59 putative metabolites, primarily flavonoids. However, we also hint at the presence of peptide and carbohydrate derivatives. The leaf extracts demonstrated the most promising metrics across all assays, exhibiting strong antioxidant and enzyme inhibitory effects as well as high levels of phenolic compounds, flavonoids, and tannins. Fingerprint analysis suggested potential peptide and carbohydrate metabolites as pancreatic lipase and α-glucosidase inhibitors. Overall, our study provides evidence on specific metabolites in Phoradendron brachystachyum that could be responsible for the therapeutic effects noted in obese and type 2 diabetes subjects.

Tannins, flavonoids, and lignin levels of clitoria ternatea L legumes in different levels of urea fertilizer and harvesting age

<p class="MDPI17abstract"><strong>Objective: </strong><em>Clitoria ternatea</em> L is a legume that has the potential as animal feed. This study determined to evaluate tannins, flavonoids, and lignin levels in different urea fertilizer applications and harvesting ages.</p><p class="MDPI17abstract"><strong>Methods: </strong>This study used a split-plot design with three replications. The main plots were urea levels (0 kg/ha, 100 kg/ha, and 200 kg/ha), as subplots were harvesting ages (30, 45, and 60 days after planting (DAP)). Fresh weights of <em>Clitoria ternatea</em> L harvested at the ages of 30, 45 and 60 DAP were weighed and recorded. The weighed forage is put in newspapers and dried in a 55°C oven for three days to gain a constant dry weight. The dried samples were then ground using a willey mill with a sieve porosity of 1 mm. Furthermore, the sample is analyzed to determine the content of secondary metabolites. The parameters observed were tannins, flavonoids, and lignin levels. Analysis of variance (ANOVA) was used to analyze Research data, and Duncan's Multiple Range (DMRT) was used to analyze the differences between means.<strong></strong></p><p class="MDPI17abstract"><strong>Results: </strong>The lowest lignin content of <em>Clitoria ternatea</em> L was prodiced in non-urea treatment (20.64± 2.53%), and significantly different compared to various levels of urea fertilization treatment (P<0.05). The highest tannin levels were obtained at 30 DAP harvest age (4.20±0.56%) and significantly different (P<0.05) from tannin levels at 45 and 60 DAP. The lowest flavonoid levels were obtained when the plants were harvested in 60 DAP (0.87±0.05%) and significantly different (P<0.05) compared to 30 and 45 DAP.<strong></strong></p><p><strong>Conclusions: </strong>The increase of urea fertilization level in <em>Clitoria ternatea</em> L only significantly affected lignin levels. The decrease of lignin content value is linear with the increase of harvesting age. In contrary, harvesting age of <em>Clitoria ternatea</em> L showed significant linear effects on tannins and flavonoids value.</p>

Pengembangan Buku Saku Berdasarkan Studi Identifikasi Tanaman Obat Pada Masyarakat Suku Serawai Bengkulu Selatan

This research is a research and development of teaching materials in the form of a pocketbook based on the study of the identification of medicinal plants in the Serawai people of South Bengkulu. The purpose of this study was to determine the medicinal plants, the content of secondary metabolite phytochemical compounds in each plant, and the validity and responses of students to the developed pocketbook. The development model used is the research and development (R&D) model of Bord and Gall (1983) which is limited to the seventh stage, namely the revision of the product from the limited trial results. From the research, 19 (nineteen) medicinal plants were selected that were used by the Serawai people of South Bengkulu. The results of secondary metabolite screening showed that the medicinal plants found contained various levels of tannins, alkaloids, saponins, flavonoids, terpenoids, and steroids. The validity of the pocketbook from the study of the identification of medicinal plants in the Serawai tribal community obtained an average value of 84.3% for the material aspect and 91.7% for the media aspect which was included in the very appropriate category. Student responses regarding the pocket book of medicinal plants developed obtained a value the percentage of material aspects, media, benefits are 85.8%, 88.0%, and 87.0%, respectively with an average of 86.9% which were included in the very good criteria. Student also give a positive response to the product being developed with a 100 % interest percentage.
 Keywords: Pocket Book, Medicinal Plants, Serawai Tribe, Secondary Metabolites

Biological potentialities and chemical composition of Tarenaya aculeata roots and stems

Plant extracts are rich in secondary metabolites responsible for numerous biological activities. This study aimed to evaluate the antioxidant, antibacterial and photoprotective potentials, toxicity and chemical composition of extracts and fractions of stems and roots of Tarenaya aculeata. Phytochemical analyses were performed at qualitative and quantitative levels to evaluate the classes of secondary metabolites. The sun protection factor (SPF) and antioxidant potentials were determined spectrophotometrically, the antibacterial activity was tested against seven bacteria and the toxicity was evaluated using Artemia salina assay. Phytochemical screening revealed the presence of alkaloids, phenolic compounds, flavonoids, glycosides, tannins and saponins. The levels of phenolic compounds, tannins and alkaloids, SPF and antioxidant potentials showed greater results in the stem (SF) and root (RF) fractions in relation to the stem (SE) and root (RE) extracts. All samples exhibited a broad spectrum of antibacterial activity, with MIC values ​​ranging from 31.25 to 250 μg mL-1. SE, SF and RF caused mortality in A. salina larvae, with LC50 of 347.06, 34.71 and 85.39 μg mL-1, respectively, whereas RE was non-toxic. Thus, T. aculeata is rich in bioactive secondary metabolites, although further studies will be needed to characterize its chemical constituents and investigate their adverse effects.

Nutritional and Phytochemical Composition of Bambara Groundnut (Vigna subterranea [L.] Verdc) Landraces in Kenya

Bambara groundnut (Vigna subterranea) is a highly nutritious legume with good drought tolerance attributes and is therefore a suitable candidate for food and nutritional security especially in drought prone areas. This study was conducted to determine the nutritional and phytochemical composition of seventeen Bambara groundnut landraces that were collected from Vihiga, Kakamega, Bungoma, Busia, and Kisumu Counties in Kenya. Prior to characterization, a field experiment was set up in Ishiara Ward in Mbeere North Sub-County in Embu County for two cropping seasons to standardize and multiply the seeds. The experiment was laid out in a Randomized Complete Block Design with three replicates. The harvested seeds were analyzed for their proximate, nutritional, and phytochemical composition using standard procedures. The moisture content ranged from 3.47 to 6.24%, total ash from 3.17 to 4.69%, crude protein from 21.18 to 26.00%, and fats from 4.56 to 7.02%. Iron levels ranged from 4.07 to 5.13 mg/100 g, sodium from 25.14 to 129.66 mg/100 g, potassium from 819.34 to 1,131.80 mg/100 g, and zinc from 0.06 to 0.42 mg/100 g. The tannin levels ranged from 0.01 to 0.04 mg/g, saponins from 0.82 to 1.06 mg/100 g, alkaloids from 0.01 to 0.12 mg/100 g, and flavonoids from 4.07 to 8.45 mg/100 g. The landraces BG-125, BS-148, and BS-145 with relatively higher nutrients composition and those with high levels of phytochemicals such as BS-104 and MU-137 are recommended to plant breeders for further selection and production of certified seeds. These selections will also be promoted to the farmers for production through various extension programs.

Fruit Characteristics of Transgenic pear (Pyrus communis L.) Trees During Long-Term Field Trials.

The quality of transgenic fruits was studied only for apple, plum and citrus. We first evaluated the transgenic fruit characteristics of pear, which is one of the most consumed fruit crops. The size, shape and biochemical composition of fruits from field-grown pear trees with marker genes were analyzed for 5 years. Soluble solids, vitamin C, and phenolic compounds varied significantly between transgenic lines, but these deviations were inconsistent. Arbutin content and sugar:acidity ratio were the most stable parameters. One transgenic line showed a stable increase in fruit weight (by 12.2-21.2%). The extremely dry and hot season increased the total phenolics (2.6-3.6 times) and tannin (3.2-3.6 times) levels, but not flavonoids. The harvest year had a stronger effect on analyzed fruit parameters than the genotype. Our study found no unintended effects of genetic transformation on pear fruit quality and confirms the importance of long-term field tests for perennial transgenic plants.

Phytochemical and biochemical composition, in vitro antioxidant potential and GC-MS analysis of red seaweeds Hypnea valentiae

The present study focused on pharmaceutical potential of red seaweed Hypnea valentiae. The phytochemical analysis of the methanolic extract of H. valentiae found more phycocompounds which was confirmed by GC-MS analysis. The significant levels of total alkaloid, phenol, tannin and flavonoid were expressed in methanolic extract of H. valentiae. In addition, the potent antioxidant activity of the methanolic extract was efficiently expressed during the total antioxidant activity, free radical scavenging assays of DPPH and hydroxyl denoted as 72.85±0.66 activity at a concentration 50-250µg/mL, 61.44±0.36 activity at a concentration 10-160µg/mL and 83.29±0.38 activity at a concentration 25-125µg/mL respectively. The results were establishing the potential of the seaweed and antioxidant properties of H. valentiae could be a valuable marine source for developing novel pharmaceutical applications.

Nutritive and Antioxidant Properties of Cocoa Placenta Obtained from Cocoa Varieties Grown in Lodjiboua (Côte d’Ivoire)

Cocoa placenta obtained after the removal of beans is a huge cocoa farm by-product usually regarded as waste by farmers. In order to contribute to their wider valorization, placenta of three varieties cocoa to know forastero, criollo and national (hybrid variety) have focused our attention. After fermentation of the placentas for 6 days, they were dried in an oven at 50° C. for 72 hours. The nutritive properties of cocoa placenta studied has give the follow results: ash (6.00 - 9.40%), proteins (7.50 - 11.40%), lipids (2.80 - 6.60%), fibers (26.20 - 36.70%) and total sugar (9.60 - 16.10%). The mineral elements contents were high with remarkable amount of K (1063.50 - 1645.12 mg/100g), Mg (196.70 - 246.89 mg/100g), Ca (96.20 - 128.41 mg/100g) and Fe (1.45 - 8.40 mg/100g). Regarding antioxidant properties, these cocoa placenta also contained appreciable levels of polyphenols (176.90 - 301.40 mgGAE/Kg), flavonoids (24.60 - 79.60 mgECE/Kg) and tannins (5.90 - 21.80 mg/Kg). The studied cocoa placenta antioxidant activity varying from 9.50 - 24.40 mmolTEq/Kg for DPPH (2.2- Diphenyl 1- picrylhydrazyl) and 22.90 - 64.30 mmolTEq/Kg for ABTS (Acide 2.2-azino-bis (3-éthylbenzothiazoline-6-sulphonique). The results of the present study clearly demonstrate that cocoa placenta is a potential by-product that need could be used in animal feed or human food formulation.

Bioaccessibility of iron and zinc in selected complementary foods fortified with micronutrient powders in Kenya

Fortification with micronutrient powders (MNPs) is recommended as a strategy for increasing the micronutrient content in complementary foods. However, plant-based diets commonly consumed in developing countries are rich in phytates and tannins, which decrease the micronutrient bioavailability. The present work analysed the relationship between the antinutrient content, and also iron and zinc bioaccessibility, in home-made MNP-fortified complementary feeding porridges refined with white rice, maize, white sorghum, finger millet, pearl millet, Irish potato, and banana samples, which were obtained from the local market and milled into flour. Porridges were prepared using the flour, cooled to 50°C, fortified with MNPs, and subjected to in vitro digestion. Total and bioaccessible zinc and iron were quantified using atomic absorption spectrometry. Tannins and phytates were analysed using Folin-Denis and high-performance liquid chromatography methods, respectively. Porridges were classified as having poor bioavailability if their phytate-zinc and phytate-iron molar ratios were > 15 and > 0.4, respectively. Only pearl millet and soybeans showed tannin levels higher than the recommended values. The lowest phytate level was observed in refined white rice (0.11 ± 0.04 g/100 g), and the highest was in pearl millet (2.83 ± 0.10 g/100 g). Zinc bioaccessibility ranged from 7.31% (finger millet) to 26.05% (corn-soy blend). Only pearl millet porridge was classified as having poor zinc bioavailability. Iron bioaccessibility ranged from 20.73% (refined white rice) to 0.62% (pearl millet). Refined white rice and Irish potato were the only foods with the phytate-iron ratio within the recommended range. Iron bioaccessibility decreased significantly with an increase in both tannin (r = -0.31, p = 0.045) and phytate (r = -0.39, p = 0.01) contents. Zinc bioaccessibility showed a significant positive relationship with tannin levels (r = 0.41, p = 0.008), but an insignificant inverse relationship with phytate levels (r = -0.072, p = 0.700). Iron bioaccessibility was adversely affected by phytate and tannin levels. To improve iron and zinc bioavailability in complementary foods, strategies for lowering the phytate and tannin contents at the household level are recommended.

Addition of Spirulina to Craft Beer: Evaluation of the Effects on Volatile Flavor Profile and Cytoprotective Properties.

SPME-GC-MS and PTR-ToF-MS techniques were applied to describe the content of volatile flavor compounds in a craft beer before and after adding spirulina. The obtained results showed that the volatile profile of the two beer samples differed. Furthermore, to chemically characterize biomass spirulina, a derivatization reaction followed by GC-MS analysis was performed, highlighting a high content of molecules belonging to different chemical classes, such as sugars, fatty acids and carboxylic acids. A spectrophotometric analysis of total polyphenols and tannins, investigation into the scavenging activity towards DPPH and ABTS radicals and confocal microscopy of brewer's yeast cells were carried out. Moreover, the cytoprotective and antioxidant properties towards the oxidative damage induced by tert-butyl hydroperoxide (tBOOH) in human H69 cholangiocytes were investigated. Finally, the modulation of Nrf2 signaling under oxidative stress conditions was also evaluated. Both samples of beer were shown to contain similar levels of total polyphenols and tannins, with slightly increased levels in that containing spirulina 0.25% w/v. Moreover, the beers were found to be endowed with radical scavenging properties towards both DPPH and ABTS radicals, albeit with a weak contribution of spirulina; however, a higher riboflavin content was detected in spirulina-treated yeast cells. Conversely, the addition of spirulina (0.25% w/v) appeared to improve the cytoprotective properties of beer towards tBOOH-induced oxidative damage in H69 cells and reduce intracellular oxidative stress. Accordingly, the cytosolic Nrf2 expression was found to be increased.