Level Of Stress Tolerance Research Articles

Metabolic engineering of low-molecular-weight antioxidants in sweetpotato

Environmental stress leads to the production of reactive oxygen species (ROS) and consequently oxidative stress, which limits plant productivity. Thus, new crop cultivars that are able to overcome oxidative stress are required to address the global issues of environmental, food, and energy security. Sweetpotato (Ipomoea batatas [L.] Lam) is considered an emerging multifunctional crop because of its high carbohydrate content and adaptation to marginal lands. Moreover, sweetpotato offers several health benefits for the aging population, as it is rich in low-molecular-weight antioxidants (LMWAs) (e.g., carotenoids, ascorbate, tocopherols, and polyphenols), dietary fiber, potassium, and minerals. We speculate that metabolic engineering of LMWAs in sweetpotato will lead to the development of smart cultivars with enhanced levels of antioxidants and abiotic stress tolerance. This review describes the current status of metabolic engineering of LMWAs in sweetpotato and the potential of sweetpotato in ensuring food and nutrition security in the twenty-first century. Here, we focus on carotenoids, ascorbate, tocopherols, and polyphenols including anthocyanins in transgenic sweetpotato plants. Additionally, we discuss the potential role of the sweetpotato Orange (IbOr) gene, involved in carotenoid accumulation and stress tolerance, in improving crop productivity under the changing global climate and alleviating the problem of vitamin A deficiency. Genetic engineering of the biosynthetic and metabolic genes of LMWAs in sweetpotato is currently under investigation via molecular breeding. We predict that metabolic engineering of antioxidants in sweetpotato will lead to the development of new cultivars with enhanced nutritional value and abiotic stress tolerance, which will ensure global food and nutrition security in the face of climate change.

Psychophysiological status of women-teachers of the higher education institution of the pension age

The psycho-emotional status of women teachers of retirement age working in the theoretical departments of the medical university has been studied. It is shown that at the end of the school year, women maintain a good level of efficiency with no signs of fatigue. At the same time, a low level of stress tolerance in combination with a high level of personal and reactive anxiety was revealed.

Brassica juncea (L.) Czern. (Indian mustard): a putative plant species to facilitate the phytoremediation of mercury contaminated soils

A Phytoremediation experimental set up was established by spiking the soil with varying concentrations of mercury (Hg) (Treatment: T1:10; T2:50; T3:100; T4:500 and T5:1,000 mg Hg/kg soil). Hg removal ability of the Indian mustard plant was determined after 30, 60 and 90 days of exposure. Hg accumulation trend in second and third month of exposure was root > leaf > stem, while for the 1st month it was root > stem > leaf. The highest percentage of Hg accumulation (81%) and glutathione (14 mg/kg) was observed in the plants of T4 and T5 treatment, respectively at 90 days of exposure indicating a high level of Hg stress tolerance. At 90 days of exposure the chlorophyll a content in leaves grown on Hg-free soil (control soil) was 1.8, 2.4, 2.8, 3.6 and 4.4 fold higher than T1, T2, T3, T4 and T5 treatment respectively. With increase in exposure duration, translocation factor decreased whereas bioconcentration factor increased signifying Hg is mainly accumulated in the roots. The study suggests that Brassica juncea can withstand under high Hg contamination and can show great potential to phytostabilize Hg when grown under 100 mg/kg of soil Hg without showing any significant detrimental effect on the plant.

Genotype-by-environment effects on grain quality among heat and drought tolerant bread wheat (Triticum aestivum L.) genotypes

ABSTRACT This study determined genotype-by-environment effect on grain quality among heat and drought tolerant bread wheat genotypes to select ideal genotypes for grain quality improvement. Gliadin (Gli), gluten (Glu), glutenin (Glut), gliadin: glutenin ratio (Gli: Glut ratio) and total protein content (TPC) were determined among 28 elite bread wheat genotypes under non-stressed and drought-stressed conditions. Additive main effect and multiplicative interaction and genotype and genotype-by-environment biplot were used for data analyses. Large genotypic effects were observed for Gli (55.42%), TPC (67.78%) and Gli: Glu ratio (49.77%), suggesting genotypic variation for these traits. High environmental influence of 89.65 and 89.06% were observed for Glu and Glut. Wheat genotypes LM03, LM22, LM23, LM41, LM44, LM60, LM62, LM71, LM73, LM71, LM85, LM90 and LM95 with high protein and protein-fractions and high levels of abiotic stress tolerance (i.e. heat and drought) were selected for breeding for high grain quality.

ПСИХОЛОГІЧНІ ОСОБЛИВОСТІ ПРОЯВІВ СТРЕСОСТІЙКОСТІ У СУЧАСНОЇ МОЛОДІ

The article is devoted to an empirical study of the psychological features of stress tolerance amongst modern youth. Such components of stress tolerance as coping behavior, adaptability, the level of self-esteem, anxiety, personality orientation, and the locus of control were researched. Based on the results of an empirical study, statistically significant indicators of differences in results in terms of stress tolerance, coping behavior in stressful situations, a tendency to unmotivated anxiety, and cognitive orientation in male and female respondents were revealed. Based on the results of comparative analysis, statistically significant indicators of differences according to the Mann-Whitney criterion and the coefficient of rank correlations of C. Spearman were discovered. Differences in the results of women and men were related to the level of stress tolerance, in particular, in men, it was significantly higher. Two statistically significant indicators of difference were found between the results of men and women in the use of certain coping strategies. Statistically significant differences in the indicators of "emotions" as a coping strategy were revealed: in the studied women, the indicators are higher than in men. Women are more emotional when exposed to stressful situations. There were also statistically significant differences in the indicators of "avoidance": men's indicators for this strategy are higher, so they are more likely to avoid stressful situations than the women studied. Statistically significant indicators of the difference were found among the results of men and women in terms of unmotivated anxiety, locus control, and neuropsychiatric stability. In the women studied, unmotivated anxiety is significantly higher. Indicators of the external locus control are significantly higher among the women studied. They are more likely to use external factors to guide their own behavior and emotional responses. Indicators of the internal locus control are significantly higher among the men studied. Men, unlike women, are more likely to use the internal resources of the individual to manage their own behavioral and emotional responses. In the result of a study of the relationship between stress resistance and adaptive capabilities, a positive correlation was found between stress resistance and neuropsychiatric stability indicators. We can determine the following trend – a low level of neuropsychiatric stability causes lower indicators of stress resistance of the individual. That is, it is quite difficult for respondents with a low level of neuropsychic constancy to manage their own emotions in stressful situations, and they put quite a lot of effort into countering a tension. Based on the results of the study, we believe that work on the development of stress tolerance among modern youth is possible only if the identified indicators are taken into account whilst developing and using appropriate preventive and correctional programs, as well as during consulting and educational psychological activities.

The Practice of the Training on Stress Tolerance Increase of Ukrainian Students in the Sphere of Legal Education

The new national curriculum implementation in the sphere of higher education in Ukraine raises the need for using tests as the means of students’ knowledge assessment. The authors have identified the connection both between the level of stress tolerance and between the level of test passing by means of using the correlated analysis, the Kolmogorov-Smirnov test and the T-Wilcoxon test. The level of stress tolerance has a serious impact upon the students’ test result. The students show a higher efficiency level while passing the test after visiting the training on stress tolerance increase. It has been identified that the opportunity value as to how to focus the students’ attention on the test is an important harbinger of the thesis that the education of Ukrainian students should be followed by the assessment of their psychological state. The authors have identified that the students’ psychological architype has an impact upon the education efficiency giving both similar and best results according to the results from other cultures.

Morphology of GFAP-Positive Cells in Male and Female Rats’ Cerebral Cortex during the Cerebral Hypoxia Development according to the Stress Tolerance Level

Correlation between individual typological features like sex-related characteristics and low initial level of stress resistance and unfavorable prognosis of ischemic brain damage is shown. At the same time, the astrocytes’ participation in neuroplasticity in this disease represents the purpose of the study. The goal was to study the cerebral cortex astrocytes’ morphometric characteristics during the cerebral hypoxia in rats of different sexes with different stress tolerance levels. We performed the study with 72 Wistar rats. According to the Open Field test results all the animals were divided into two subgroups: with high and low level of stress tolerance. Both carotid arteries of the experimental group animals (48 animals) were bandaged. Animals were removed from the experiment at 21, 60 and 90 days after surgery. Glial fibrillary acidic protein (GFAP), a marker of mature astrocytes, was detected on histological sections of the central brain gyrus using primary polyclonal rabbit antibodies. Progressive decrease of the astrocytes’ numerical density and the number of first order processes were obtained during the study. It was less pronounced in animals with high stress tolerance and females. Increase of the processes’ distribution area was reliably detected. Area decreased after 90th day of the experiment. It is concluded that the astrocytes’ alteration develops earlier in animals with high stress tolerance and males, later in females (60 days) and animals with low stress tolerance (90 days).

Psychological features of cadets with military professional maladjustment

The psychological characteristics of professionally maladapted cadets are considered. For signs of maladjustment were taken: performance - 3,5 points and below; physical fitness - 3 points and below; high incidence: the number of days of work loss from the beginning of the school year - 11 days or more; low discipline; poor relationship with the command and with colleagues, low psychological stability. Psychological personality traits can both contribute to the military-professional adaptation, and hinder it. Well-adapted cadets for training in a military college are characterized by a high level of stress tolerance, they are curious, have flexible thinking, they have hyperthymic characterological features. Professionally maladjusted cadets are characterized by stereotyped thinking, the prevalence of anxious and exalted character traits, the use of physical force against another person, readiness for the manifestation of negative feelings with the slightest excitement (hot temper), suspicion of relations with others, the expression of negative feelings as through form (cry , screeching), and through the content of verbal responses (curses, threats), the oppositional manner in behavior from passive resistance to active struggle From established customs and laws, they are more aggressive. Based on discriminant modeling, a highly informative forecast model of military professional maladjustment of cadets was developed as part of medical and psychological support measures (Wilks lambda: 0,29207 approx. F (4,58)=35,146; p

Functional Analysis of Pepper F-box Protein CaDIF1 and Its Interacting Partner CaDIS1: Modulation of ABA Signaling and Drought Stress Response.

Plant adaptive responses to environmental stress are coordinated by inhibition of plant growth and development. Abscisic acid (ABA) is a major phytohormone that regulates the stress response, and its sensitivity determines stress tolerance levels. In this study, we report the identification and functional role of a novel F-box protein, CaDIF1 (Capsicum annuum Drought-Induced F-box Protein 1). The expression of CaDIF1 in pepper leaves was induced by ABA, drought, H2O2, and NaCl treatments. In comparison with wild-type pepper plants, CaDIF1-silenced pepper plants exhibited a drought-sensitive phenotype, whereas CaDIF1-overexpressing (OX) plants exhibited ABA-sensitive and drought-tolerant phenotypes. Using a yeast two-hybrid assay, we identified CaDIS1 (C. annuum DIF1-Interacting SKP 1), which interacts with CaDIF1 in the cytoplasm and nucleus. Consistent with CaDIF1-silenced pepper plants, CaDIS1-silenced pepper plants displayed ABA insensitivity and reduced drought tolerance, and these were characterized by larger stomatal apertures and greater transpirational water loss. Taken together, our results indicate that CaDIF1 and its interacting partner CaDIS1 synergistically regulate the ABA-dependent defence signaling response to drought stress.

Small RNA and degradome deep sequencing reveal respective roles of cold-related microRNAs across Chinese wild grapevine and cultivated grapevine

BackgroundChinese wild grapevine (Vitis amurensis) has remarkable cold stress tolerance, exceeding that of the common cultivated grapevine (Vitis vinifera L.).ResultHere, we surveyed the expression dynamics of microRNAs (miRNAs) across Chinese wild grapevine (cv. Beibinghong) and cultivated grapevine (cv. Cabernet Sauvignon) under cold stress using high-throughput sequencing. We identified 186 known miRNAs in cultivated grape and 427 known miRNAs in Beibinghong. Of the identified miRNAs, 59 are conserved miRNAs orthologous in Cabernet Sauvignon and Beibinghong. In addition, 105 and 129 novel miRNAs were identified in Cabernet Sauvignon and Beibinghong, respectively. The expression of some miRNAs was related to cold stress both in Cabernet Sauvignon and Beibinghong. Many cold-related miRNAs in Cabernet Sauvignon and Beibinghong were predicted to target stress response-related genes such as MYB, WRKY, bHLH transcription factor genes, and heat shock protein genes. However, the expression tendency under cold treatment of many of these miRNAs was different between Cabernet Sauvignon and Beibinghong. Different modes of expression of cultivated and Chinese wild grape miRNAs were indicated in key pathways under cold stress by degradome, target prediction, GO, and KEGG analyses.ConclusionOur study indicated three likely reasons that led to the different cold stress tolerance levels of Cabernet Sauvignon and Beibinghong. Specifically, there may be (1) differential expression of orthologous miRNAs between cultivated grapevine and Chinese wild grape; (2) species-specific miRNAs or target genes; or (3) different regulatory models of miRNAs in cultivated and Chinese wild grape in some key pathways.

Alleviation of Phytophthora infestans Mediated Necrotic Stress in the Transgenic Potato (Solanum tuberosum L.) with Enhanced Ascorbic acid Accumulation.

Potato is the most widely cultivated non-cereal crop in the world, and like any other crop, it is susceptible to yield losses because of various factors, including pathogen attacks. Among the various diseases of potato, late blight caused by the oomycete Phytophthora infestans is considered as the most devastating disease worldwide. In this study, transgenic potato plants overexpressing the D-galacturonic acid reductase (GalUR) gene with an enhanced level of cellular L-ascorbate (L-AsA) were challenged with Phytophthora infestans to determine the level of stress tolerance induced in those plants. With the onset of pathogen infection, necrotic lesions progressively expanded and became necrotic in the control plants. The transgenic potato lines with enhanced ascorbic acid showed reduced necrotic lesions. Hydrogen peroxide (H2O2) and malondialdehyde (MDA) levels were relatively lower in transgenic plants compared to the untransformed control (UT) plants. The mRNA expressions of pathogenesis-related (PR) genes, such as pathogenesis related 1 (PR1) and phenylalanine ammonia-lyase (PAL) were slightly higher in GalUR overexpressing transgenic lines as compared to the untransformed control plants. Pathogen infection also altered the mRNA expression of genes associated with gibberellic acid (GA) and abscisic acid (ABA) biosynthesis. Furthermore, the increase in various antioxidant enzymes was also observed in the gene expression analysis with the transgenic plants. The complete loss of the pathogen growth and disease occurrence was not observed in our study; however, the findings indicated that an increase in the level of cellular L-ascorbate in the transgenic potato leads to enhanced cellular antioxidants, PR genes and plant defense hormones, such as GA and ABA resulting in the reduction of the disease symptoms caused by the Phytophthora infestans.

PREVENTION OF POSTPARTUM COMPLICATIONS IN WOMEN WITH A LARGE INTERGENETIC INTERVAL

Prevention and treatment of complications of the postpartum period remains one of the urgent tasks of modern obstetrics.Objective: to evaluate the effectiveness of the treatment and prophylactic complex to reduce the frequency of postpartum complications in women with a large intergenetic interval.Materials and methods. 197 pregnant women and parturients were examined: 85 women with a large intergenetic interval - 1 group; 68 age-olds for the first time - 2 group and 44 women giving birth with a break from 3 to 5 years - 3 group.The developed treatment-and-prophylactic complex was applied in 30 women with a large intergenetic interval (main group) and in 37 women who were observed in accordance with generally accepted events (comparison group). The average age of women ranged from 26 to 45 years.The study used clinical, psychological, bacteriological, instrumental (ultrasound), immunological, statistical research methods.Results. In the postpartum period, in women with a large intergenetic interval, violations of uterine involution in puerperia are observed (subinvolution - 9.4%, lochyometer - 7.1%). During the year, these women have a low level of stress tolerance (70%), short lactation (up to six months 54%), prolonged recovery of menstrual function (37.5% - more than a year), insufficient education regarding modernmethods of contraception (> 75% - interrupted sexual intercourse).The proposed complex allowed to reduce the incidence of puerperium complications (subinvolution and lochiometer were not observed) and reproductive health disorders during the year after delivery (recovery of menstrual function indicators in 6-12 months - 70%, lactation duration more than 6 months - 58%, use of modern methods contraception up to 70%). A high level of stress tolerance was in 60% of women in the main group.Conclusion. Women with a large intergenetic interval have complications after childbirth and therefore require careful obstetric and gynecological support both during pregnancy and during the year after childbirth.

Molecular profiling in relation to drought tolerance in advance breeding lines of rice using microsatellite markers

Using agro-morphological characters and microsatellite markers, advance breeding lines of rice were discriminated for their ability to tolerate drought stress at reproductive stage. Experimental materials consisting of 17 advance breeding lines and a check were evaluated in randomized block design with three replications under irrigated condition and drought condition created under rainout shelter during three consecutive years. An analysis of variance revealed significant differences among the genotypes for all the ten agro-morphological characters evaluated under both the conditions across the years. Principal component analysis showed the relative importance of root length, number of tillers per plant, number of grains per panicle, harvest index and grain yield per plant among agro-morphological characters and stress tolerance level, stress susceptibility index, stress tolerance index and drought tolerance efficiency among drought tolerance indices as the important classification variables. Relative mean performance in respect of grain yield as well as drought tolerance indices reflected remarkably greater degree of drought tolerance in 11 advance breeding lines and the check, discriminating them from remaining entries under evaluation. Utilizing a panel of 32 microsatellite primers, selective amplification of targeted genomic regions revealed that the primers RM 72, RM 163, RM 212, RM 225, RM 231, RM 302, RM 327, RM 518, RM 521, RM 555, RM 1349, RM 3549 and RM 5443 were highly informative with greater gene diversity and discrimination ability. Hierarchical cluster analysis based on molecular profiles discriminated the entries into five genotypic groups and drought tolerant entries were accommodated into three distinct groups with remarkably greater efficiency (85.7%). Principal coordinate analysis based two dimensional plots of microsatellites dependent genetic profiles displayed a very close correspondence with the genotypic clustering pattern revealed from a perusal of dendrogram. Sequential exclusion of primers in cluster analysis led to identification of RM 212, RM 231, RM 324, RM 431, RM 521, RM 3549 and RM 6374 as the most useful primers for discrimination of drought tolerant and susceptible lines of rice. Molecular profiling based on these markers can be utilized as efficient tools for discrimination and identification of drought tolerant lines.

Involvement of polyamines in sucrose-induced tolerance to atrazine-mediated chemical stress in Arabidopsis thaliana

Treatment of Arabidopsis thaliana seedlings with the PSII-inhibiting herbicide atrazine results in xenobiotic and oxidative stress, developmental arrest, induction of senescence and cell death processes. In contrast, exogenous sucrose supply confers a high level of atrazine stress tolerance, in relation with genome-wide modifications of transcript levels and regulation of genes involved in detoxification, defense and repair. However, the regulation mechanisms related to exogenous sucrose, involved in this sucrose-induced tolerance, are largely unknown. Characterization of these mechanisms was carried out through a combination of transcriptomic, metabolic, functional and mutant analysis under different conditions of atrazine exposure. Exogenous sucrose was found to differentially regulate genes involved in polyamine synthesis. ARGININE DECARBOXYLASE ADC1 and ADC2 paralogues, which encode the rate-limiting enzyme (EC 4.1.1.19) of the first step of polyamine biosynthesis, were strongly upregulated by sucrose treatment in the presence of atrazine. Such regulation occurred concomitantly with significant changes of major polyamines (putrescine, spermidine, spermine). Physiological characterization of a mutant affected in ADC activity and exogenous treatments with sucrose, putrescine, spermidine and spermine further showed that modification of polyamine synthesis and of polyamine levels could play adaptive roles in response to atrazine stress, and that putrescine and spermine had antagonistic effects, especially in the presence of sucrose. This interplay between sucrose, putrescine and spermine is discussed in relation with survival and anti-death mechanisms in the context of chemical stress exposure.

Isolation and characterization of water-deficit stress-responsive α-expansin 1 (EXPA1) gene from Saccharum complex.

In this study, full-length (1282-1330bp) α-expansin 1 (EXPA1) gene from three different accessions belonging to Saccharum complex (Saccharum officinarum-SoEXPA1, Erianthus arundinaceus-EaEXPA1, and Saccharum spp. hybrid-ShEXPA1) was isolated using RAGE technique and characterized. The intronic and coding regions of isolated expansin genes ranged between 526-568 and 756-762bp, respectively. An open reading frame encoding a polypeptide of 252 amino acids was obtained from S. officinarum and commercial sugarcane hybrid, whereas 254 amino acids were obtained in E. arundinaceus, a wild relative of Saccharum. Bioinformatics analysis of deduced protein revealed the presence of specific signature sequences and conserved amino acid residues crucial for the functioning of the protein. The predicted physicochemical characterization showed that the protein is stable in nature with instability index (II) value less than 40 and also clearly shown the dominance of random coil in the protein structure. Phylogenetic analysis revealed high conservation of EXPA1 among Saccharum complex and related crop species, Sorghum bicolor and Zea mays. The docking study of EXPA1 protein showed the interaction with xylose, which is present in xyloglucan of plant cell wall, elucidated the role of the expansin proteins in plant cell wall modification. This was further supported by the subcellular localization experiment in which it is clearly seen that the expansin protein localizes in the cell wall. Relative expression analysis of EXPA1 gene in Saccharum complex during drought stress showed high expression of the EaEXPA1 in comparison with SoEXPA1 and ShEXPA1 indicating possible role of EaEXPA1 in increased water-deficit stress tolerance in E. arundinaceus. These results suggest the potential use of EXPA1 for increasing the water-deficient stress tolerance levels in crop plants.

A Comparison of Emotion Regulation Difficulties, Distress Tolerance and Affect Balance among Imprisoned Women Accused of Fraud and Ordinary Women

Background: Differences among ordinary and imprisoned people are in terms of both physiologic and biologic properties and mental and social characteristics.Objectives: This study aims to compare the emotion regulation difficulties, distress tolerance and affect balance between ordinary and imprisoned women accused of fraud to clarify the importance of emotion regulation and the recognition of people's excitement in improving the quality of healthy life.Methods: This research is an applied work conducted by the causal-comparative method. The statistical universe of this study consists of 120 individuals including 50 imprisoned women accused of fraud in Rey Prison as well as 70 ordinary women without any criminal records that were randomized from Tehran. The research tools are as follows: Difficulties in Emotion Regulation Scale (DERS), Distress Tolerance Scale (DTS) and Affect Balance Scale (ABS). Results: The results show a difference between the difficulties of emotion regulation, distress tolerance and affect balance of women accused of fraud and ordinary people. Our results show that women accused of fraud compared to ordinary women according to the value (t = 37.11) at a significance level of 0.01 are at a lower level in their emotional settings. Also due to the amount (t = -11.68), a higher level of distress and stress tolerance of ordinary women compared to the defendants was shown and according to the value (t = -4.83), there is a significant difference in the affectwhere female fraudsters are at a lower level versusordinary ones.Conclusion: The imprisoned women should be paid specially into attention in terms of emotion regulation, distress tolerance and affect balance.

Screening of Rice Apoplast Associated Endophytic Bacterial Isolates for Moisture Stress Tolerance and Plant Growth Promoting Traits

Investigations were made to identify, screen and characterize rice apoplastic fluid endophytic bacterial strains for osmotic stress tolerance and plant growth promoting activity. These bacterial strains were identified phylogentically as Bacillus subtilis TSAC5, Bacillus subtilis TSAA2, Bacillus sp. R2AA10, Bacillus cereus R2AA7, Bacillus marisflavi TSAC7, Cupriavidus alkaliphilus TSAC1, Delftia sp. TSAC2, Janibacter melonis R2AA1, Microbacterium oleivorans R2AA6, Kocuria rosea R2AA5, Pseudomonas aeruginosa NAAI3 and Sphingobium yanoikuyae R2AI1. Of these 12 strains, eight were obtained from drought tolerant rice varieties such as Anna R4 and IR DRT 64 and four from normal rice variety CO51. These endophytes exhibited varied level of osmotic stress tolerance created using different concentrations of PEG 6000 (-1.5, -3.0, -4.0, -4.5, -5.0 and -5.5 MPa) under in vitro conditions in liquid and solid medium. Among the 12 strains, Cupriavidus alkaliphilus TSAC1, Delftia sp. TSAC2, Bacillus marisflavi TSAC7, Janibacter melonis R2AA1, Kocuria rosea R2AA5, Pseudomonas aeruginosa NAAI3 and Sphingobium yanoikuyae R2AI1 showed moisture stress tolerance up to -5.5 MPa. Further increase in moisture stress of -6.0 MPa inhibited growth of 12 tested strains. These strains also exhibited Indole acetic acid (IAA), gibberellic acid (GA3) and extracellular polysaccharide (EPS) production and also showed ACC deaminase (ACCD) activity. Maximum extracellular proline production was found in Bacillus marisflavi TSAC7 under both normal and water stressed conditions. Higher IAA productivity was registered in Janibacter melonis R2AA1 followed by Cupriavidus alkaliphilus TSAC1 and Sphingobium yanoikuyae R2AI1 in the absence of PEG 6000. In the presence of PEG 6000 Delftia sp. TSAC2 recorded maximum IAA productivity. Bacillus subtilis TSAA2 showed greater ACC deaminase activity. EPS production was in the order of Pseudomonas aeruginosa NAAI3 followed by Sphingobium yanoikuyae R2AI1. Sphingobium yanoikuyae R2AI1 produced maximum quantity of GA3 both under normal and water stressed condition. None of the tested strains indicated insoluble phosphate, silicate and zinc solubilisation.

Effect of progressive muscle relaxation with analgesic on anxiety status and pain in surgical patients.

surgery is a stressful experience for patients and most surgical patients have some degree of anxiety. The purpose of this study was to investigate the effect of a relaxation technique in addition to narcotic analgesic on health promotion in surgical patients. in this clinical trial, 70 patients who were candidates for elective upper and lower gastrointestinal system surgery were selected. They were randomly divided into two groups: case (morphine 0.15 mg/kg daily in divided doses and progressive muscle relaxation (PMR)) and control (morphine 0.15 mg/kg daily in divided doses). The intervention group (case group) performed PMR from 6 hours before surgery until 24 hours after surgery. Vital signs and anxiety were evaluated in the two groups after surgery. Data were analysed by t-test, analysis of variance, and chi-square test. a statistically significant difference was seen in vital signs, pain and anxiety between the two groups. However, there was also a significant difference between them in terms of economic status and insurance coverage, which could have had an effect on stress and anxiety. PMR could increase the pain threshold, stress and anxiety tolerance and adaptation level in surgical patients. Therefore, using this technique could be an appropriate way to reduce analgesic drug consumption.

Trehalose increases the oxidative stress tolerance and biocontrol efficacy of Candida oleophila in the microenvironment of pear wounds

Harvested fruit tissues produce reactive oxygen species (ROS) in response to wounding. ROS production is further elevated when biocontrol yeasts are introduced into the wounds, where they act as a bio-elicitor and this results in an environment in which the biocontrol yeast is subject to oxidative stress. In the present study, the effect of trehalose, a non-reducing disaccharide and a major reserve carbohydrate, on the level of oxidative stress tolerance of the biocontrol yeast Candida oleophila was examined in wounded pear fruits. Results indicated that the level of trehalose in C. oleophila yeast cells increased when they were cultured in a trehalose-containing medium. Exposure to a trehalose-medium and the increase in internal trehalose improved the adaptation of C. oleophila to the oxidative environment present in pear fruit wounds. Trehalose-treated cells exhibited a lower accumulation of ROS and mitochondrial impairment compared to untreated, control cells when they were introduced into wounded pear fruit. The trehalose treatment up-regulated the expression of antioxidant genes, including peroxisomal catalase and thioredoxin reductase, in yeast cells. Trehalose-treated yeast also exhibited a faster growth rate in pear wounds and greater biocontrol efficacy against blue mold (Penicillium expansum) and Alternaria rot (Alternaria alternata) than untreated, control yeast. These results support the premise that trehalose improves the biocontrol efficacy of C. oleophila via the activation of antioxidant defense systems which support a faster rate of growth of the yeast in wounds of pear fruit.

Исследование стрессоустойчивости и жизнестойкости сотрудников уголовно-исполнительной системы

The article presents the results of a psychological survey of 90 employees, who determined the level of organizational stress and a degree of development of two professionally important qualities - stress tolerance and resilience. Three psychodiagnostic methods were used: 1) K. McLean scale of organizational stress in N. Vodopyanova's adaptation; 2) the method of studying stress tolerance as a socio-psychological quality of a person; 3) S. Muddy test of resilience. It was found that about 75% of employees deferred by a low overall level of organizational stress and at the same time they had a high level of stress tolerance and resilience; 17% of employees demonstrated a high level of organizational stress, 6% of which was characterized by a low level of stress tolerance. The consistent patterns are established as a result of the correlation analysis: the more active and productive an employee is, the higher his resilience and vice versa; the older the employee, the lower his control indicator; with increasing work experience, flexibility in employee behavior increases. The results of a comparative analysis by gender mark significant differences in the overall level of stress tolerance (higher among women) and the ability of self-knowledge (more developed among men). The psychological conditions for increasing stress tolerance and resilience on an individual level, as well as social and psychological conditions for reducing organizational stress are described.