Levels Of Resistant Starch Research Articles

Resistant starch levels and in vitro starch digestibility of selected cooked Philippine brown and milled rices varying in apparent amylose content and glycemic index

Resistant starch (RS) content, starch digestibility, and hydrolysis index (HI) were analyzed in vitro for four selected Philippine rice varieties varying in apparent amylose content (AC) and glycemic index (GI), in cooked brown and milled rice forms. Starch digestibility curves were studied in relation to AC and reported GI values. Brown and milled rices of Improved Malagkit Sungsong 2 (IMS2), NSIC Rc160, IR64, and PSB Rc10 were cooked on separate beakers placed in automatic electric rice cookers and based on pre-determined water:rice ratios. RS levels of cooked milled rices ranged from 0.15 to 0.99% (mean = 0.45%). Their corresponding cooked brown rices had RS contents ranging from 0.24 to 1.61% (mean = 1.05%), with PSB Rc10 having the highest levels in both forms. HI ranged from 59.3 to 102.2%, with the highest noted for the waxy rice, IMS2, while corresponding brown rices had significantly lower HI spanning 49.2–66.9%. Previously reported GI values of these varieties were positively correlated with HI and estimated GI in this study. RS and non-resistant starch levels, and HI were highly correlated with AC. In vitro starch digestibility studies, as related to AC and GI, may be useful in screening for rice grain and nutritional properties aimed at developing new varieties with desirable quality and enhanced nutritional and functional properties.

Generation and Starch Characterization of Non-Transgenic BEI and BEIIb Double Mutant Rice (Oryza sativa) with Ultra-High Level of Resistant Starch

BackgroundCereals high in resistant starch (RS) are gaining popularity, as their intake is thought to help manage diabetes and prediabetes. Number of patients suffering from diabetes is also increasing in Asian countries where people consume rice as a staple food, hence generation of practically growable high RS rice line has been anticipated. It is known that suppression of starch branching enzyme (BE) IIb increases RS content in cereals. To further increase RS content and for more practical use, we generated a non-transgenic be1 be2b double mutant rice (Oryza sativa) line, which completely lacked both proteins, by crossing a be1 mutant with a be2b mutant.ResultsThe be1 be2b mutant showed a decrease in intermediate amylopectin chains and an increase in long amylopectin chains compared with be2b. The amylose content of be1 be2b mutant (51.7%) was the highest among all pre-existing non-transgenic rice lines. To understand the effects of chewing cooked rice and cooking rice flour on RS content, RS content of mashed and un-mashed cooked rice as well as raw and gelatinized rice flour were measured using be1 be2b and its parent mutant lines. The RS contents of mashed cooked rice and raw rice flour of be1 be2b mutant (28.4% and 35.1%, respectively) were 3-fold higher than those of be2b mutant. Gel-filtration analyses of starch treated with digestive enzymes showed that the RS in be1 be2b mutant was composed of the degradation products of amylose and long amylopectin chains. Seed weight of be1 be2b mutant was approximately 60% of the wild type and rather heavier than that of be2b mutant.ConclusionsThe endosperm starch in be1 be2b double mutant rice were enriched with long amylopectin chains. This led to a great increase in RS content in cooked rice grains and rice flour in be1 be2b compared with be2b single mutant. be1 be2b generated in this study must serve as a good material for an ultra-high RS rice cultivar.

Development and characterization of green banana-based fondant icing

ABSTRACT The insertion of functional foods in the confectionery industry is a growing reality, aiming to meet a growing demand for products that are visually attractive and at the same time healthy. The present study aimed to develop and characterize the physical, physicochemical, microbiological, and sensory properties of fondant icings based on green banana biomass and flour. Two formulations were developed (NFBI - Neutral Flavor Biomass-based Icing, and CFBI - Chocolate Flavor Biomass-based Icing), which were compared with the commercial fondant icings (NFCI - Neutral Flavor Commercial Icing, and CFCI - Chocolate Flavor Commercial Icing). The fondant icings were evaluated for centesimal composition, caloric value, physical, physicochemical (resistant starch, titratable acidity, water activity, texture, and color), and microbiological characteristics, as well as sensory acceptance. The icings based on green banana biomass and flour showed higher levels of ash, fiber, resistant starch, and protein, as well as lower total caloric value, and thus, were healthier. These icings were also softer and had more intense color compared to commercial ones, as well as good sensory acceptance, especially the one with chocolate flavor, which had greater than 72% approval. Microbiological analyses indicated that the icings could be stored under refrigeration (<4 ºC) for 30 days. Given the nutritional quality and health benefits, the icings prepared with green banana biomass and flour proved to be promising and could serve a niche market represented by people who seek a healthier diet or need to control their sugar consumption, such as diabetics.

Influence of potato Variety on Phenolic Content, <i>In-vitro</i> Starch Digestibility and Predicted Glycaemic Index of Crisps and Chips from Nyandarua County, Kenya

With the changing of lifestyles globally, the demand for ready-to-eat (RTE) foods has increased. However, most of these RTE foods have been associated with intermediate (55-70) to high glycaemic index (GI) (>70) linked to high incidences of type 2 diabetes. Nyandarua County in Kenya is a major producer and consumer of potato and has the second highest type 2 diabetes prevalence (10.8%). Therefore, there is need to investigate whether there is a relationship between the potato and potato products consumed and the high type 2 diabetes prevalence. Total phenolic content (TPC), dry matter, and the levels of rapidly digestible starch (RDS), slowly digestible starch (SDS) and resistant starch (RS) may vary depending on potato variety and the form of the product, and may affect the rate and extent of starch digestibility, which affects the GI. This study investigated the effects of variety and processing method (product form) on the levels of TPC, dry matter, RDS, SDS, RS and GI in chips and crisps prepared from 3 potato varieties (Shangi, Dera mwana and Dutch Robijn). Potato variety significantly affected TPC, RDS, SDS and GI but did not significantly affect RS (p>0.05). Processing method results in different product forms which significantly affected dry matter content and GI (p<0.05). Higher levels of TPC and lower scores of GI were found in chips and crisps prepared from Dera mwana variety. Significant positive correlation relationships were observed between GI, and RDS and SDS (p < 0.05), and RDS and SDS (p<0.05). This study recommends reduced consumption of chips prepared from Shangi in favour of Dera mwana variety which has better potential for glycemic control.

Evaluation of an Ileorectostomised Rat Model for Resistant Starch Determination.

The human ileostomy model, widely considered the benchmark for determining in vivo starch digestibility, has disadvantages. The ileorectostomised rat model (IRM) is a possible surrogate but evidence as to its validity is scant. In this preliminary study, the resistant starch (RS) content of test breads made from refined low (LAW-R) and high amylose wheat (HAW-R) flours was established in a randomised cross-over trial involving six human ileostomy participants. Starch digestibility of refined breads and diets made from these flours was then evaluated in ileorectostomised rats using a similar experimental format. Physical performance measures and other data were also collected for the rat model. The amount of RS in the low- and high-amylose breads as measured using the human model was 0.8 ± 0.1 and 6.5 ± 0.3 g/100 g, respectively. The RS level of HAW-R bread determined using ileorectostomised rats was 5.5 ± 0.8 g/100 g, about 15% less than that recorded in the human study, whereas for conventional wheat breads the models produced similar RS values. While offering promise, further validation using a wide variety of starchy food products is needed before the IRM can be considered an acceptable alternative for RS determination.

High Levels of Prebiotic Resistant Starch in Diet Modulate a Specific Pattern of miRNAs Expression Profile Associated to a Better Overall Survival in Pancreatic Cancer.

Dietary patterns are well known risk factors involved in cancer initiation, progression, and in cancer protection. Previous in vitro and in vivo studies underline the link between a diet rich in resistant starch (RS) and slowing of tumor growth and gene expression in pancreatic cancer xenograft mice. The aim of this study was to investigate the impact of a diet rich in resistant starch on miRNAs and miRNAs-target genes expression profile and on biological processes and pathways, that play a critical role in pancreatic tumors of xenografted mice. miRNA expression profiles on tumor tissues displayed 19 miRNAs as dysregulated in mice fed with RS diet as compared to those fed with control diet and differentially expressed miRNA-target genes were predicted by integrating (our data) with a public human pancreatic cancer gene expression dataset (GSE16515). Functional and pathway enrichment analyses unveiled that miRNAs involved in RS diet are critical regulators of genes that control tumor growth and cell migration and metastasis, inflammatory response, and, as expected, synthesis of carbohydrate and glucose metabolism disorder. Mostly, overall survival analysis with clinical data from TCGA (n = 175) displayed that almost four miRNAs (miRNA-375, miRNA-148a-3p, miRNA-125a-5p, and miRNA-200a-3p) upregulated in tumors from mice fed with RS were a predictor of good prognosis for pancreatic cancer patients. These findings contribute to the understanding of the potential mechanisms through which resistant starch may affect cancer progression, suggesting also a possible integrative approach for enhancing the efficacy of existing cancer treatments.

Graded dietary resistant starch concentrations on apparent total tract macronutrient digestibility and fecal fermentative end products and microbial populations of healthy adult dogs.

Resistant starch (RS) is fermentable by gut microbiota and effectively modulates fecal short-chain fatty acid concentrations in pigs, mice, and humans. RS may have similar beneficial effects on the canine gut but has not been well studied. The objective of this study was to evaluate the effects of 0%, 1%, 2%, 3%, and 4% dietary RS (Hi-maize 260) on apparent total tract macronutrient digestibility, fecal characteristics, fermentative end-product concentrations, and microbiota of healthy adult dogs. An incomplete 5 × 5 Latin square design with seven dogs and five experimental periods was used, with each treatment period lasting 21 d (days 0 to 17 adaptation; days 18 to 21 fresh and total fecal collection) and each dog serving as its own control. Seven dogs (mean age = 5.3 yr; mean body weight = 20 kg) were randomly allotted to one of five treatments formulated to be iso-energetic and consisting of graded amounts of 100% amylopectin cornstarch, RS, and cellulose and fed as a top dressing on the food each day. All dogs were fed the same amount of a basal diet throughout the study, and fresh water was offered ad libitum. The basal diet contained 6.25% RS (dry matter [DM] basis), contributing approximately 18.3 g of RS/d based on their daily food intake (292.5 g DM/d). Data were evaluated for linear and quadratic effects using SAS. The treatments included 0%, 1%, 2%, 3%, and 4% of an additional RS source. Because Hi-maize 260 is approximately 40% digestible and 60% indigestible starch, the dogs received the following amounts of RS daily: 0% = 18.3 g (18.3 + 0 g), 1% = 20.1 g (18.3 + 1.8 g), 2% = 21.9 g (18.3 + 3.6 g), 3% = 23.7 g (18.3 + 5.4 g), and 4% = 25.5 g (18.3 + 7.2 g). Apparent total tract DM, organic matter, crude protein, fat, and gross energy digestibilities and fecal pH were linearly decreased (P < 0.05) with increased RS consumption. Fecal output was linearly increased (P < 0.05) with increased RS consumption. Fecal scores and fecal fermentative end-product concentrations were not affected by RS consumption. Although most of the fecal microbial taxa were not altered, Faecalibacterium were increased (P < 0.05) with increased RS consumption. The decrease in fecal pH and increase in fecal Faecalibacterium would be viewed as being beneficial to gastrointestinal health. Although our results seem to indicate that RS is poorly and/or slowly fermentable in dogs, the lack of observed change may have been due to the rather high level of RS contained in the basal diet.

In Vitro Glycemic Response of Indigenous Pigmented Rice Cultivars from South India and Influence of Different Carbohydrate Components

Staple diet patterns such as white rice consumption play an important part in the occurrence of chronic lifestyle-related disease like diabetes. This study intended to identify pigmented rice cultivars from India as an alternative to white rice. Nine carbohydrate components were quantified in six red and two black pigmented rice varieties. In vitro starch digestibility was also analyzed and the predicted glycemic index (pGI) was estimated. The relationship between the carbohydrate components and the pGI of the rice varieties was analyzed. The rice varieties, Kattuyanam and Chennangi exhibited high levels of insoluble dietary fibre, total dietary fibre and amylose. High soluble dietary fibre contents were observed in Poonghar and Aruvadam kuruvai. The levels of resistant starch and slowly digestible starch were found to be the highest in Karupakavuni. The results indicated Kattuyanam, Chennangi, Karungkuruvai, and Poonghar to be low pGI rice varieties (&lt; 55). The three varieties, Kesari, Karupakavuni, and Aruvadam kuruvai were categorized under the medium pGI category (56-69). Mapillai samba had a high pGI of 70 which could be due to processing (partially milled and parboiled), but had significantly lower pGI than the white rice, Sona masuri (GI – 76). The dietary fibre components, resistant starch, slowly digestible starch, and the amylose content were negatively correlated with the pGI. Among these components, the insoluble dietary fibre, total dietary fibre, and resistant starch with significantly high (p ≤0.01) correlation were found to be the major determining factors of pGI in the studied pigmented rice cultivars. The inclusion of the identified pigmented rice varieties with low to medium glycemic response in the diet could become a key role in the dietary management of diabetes, especially among the rice-eating population.

Enhancing resistant starch content in brown rice using supercritical carbon dioxide processing

AbstractDietary fiber and resistant starch (RS) play a key role in suppressing the increase in blood glucose levels. Whole grains such as brown rice (BR) which have a higher content of dietary fiber and important nutrients, are common products used for the maintenance of human health. The higher RS content in BR is suggested to contribute significantly to blood glucose level maintenance in cooperation with dietary fiber. We aimed to enhance the RS levels in BR using supercritical carbon dioxide (scCO2) processing which is typically undertaken by the reactor packed with BR and filled carbon dioxide in a state of supercritical fluids developed with above critical temperature and pressure, and a suitable operating duration as an alternative to conventional methods. This innovative process expectedly enhanced the RS content, promoted gelatinization, and eliminated the foul odors causing appetizing control. In addition, this processing method is highly effective for the removal of pollutions such as pesticides and inactivation of microbial contaminants in food. Therefore, scCO2 processing is a promising alternative to conventional methods.Practical ApplicationProcessing RS in cereals using scCO2 is an innovative alternative to conventional processes such as heat‐moisture treatment and annealing, autoclaving and retrogradation cycles, high hydrostatic pressure, and extrusion that are commonly used to promote RS formation in food products to improve human health and consequently contributes to the control of blood glucose levels after a meal. The innovative scCO2 process is considerably simple, easily operable, and environmentally friendly and safe due to the use of nontoxic CO2 as a solvent. This process has great potential for application in the extraction of pesticides and fats and the inactivation of microbes in food products. Therefore, scCO2 processing will be useful as a food processing method that helps maintain human health.

Physical and chemical characteristics of enzymatically modified Batu banana (Musa balbisiana Colla) and Kepok banana (Musa paradisiaca formatypica) flours

Batu (Musa balbisiana Colla) and Kepok (Musa paradisiaca formatypica) bananas are two food commodities with high resistant starch content. The later has been suggested to prevent colorectal cancer. Modifications of these, into flour via the addition of pullulanase enzymes and autoclaving-cooling method are expected to improve the quality. The physical characteristics, levels of resistant starch, amylose, amylopectin and in vitro digestibility of enzymatically modified banana starches were determined. Therefore, a descriptive study was performed with untreated Batu banana flour (TP), autoclavingcooling (AC), autoclaving-cooling + pullulanase enzyme (AC + E), and autoclavingcooling + pullulanase enzyme + autoclaving-cooling (AC + E + AC). The granular morphology, water absorption, and swelling ability were evaluated as physical characteristics. Amylose, amylopectin content, and starch digestibility were evaluated as chemical characteristics. Based on SEM, the analysis of granular morphology showed structural modification in both treatments, although Batu banana flour showed greater water absorption and swelling ability, with and without AC + E + AC. However, both samples demonstrated better outcomes, in terms of high levels of starch resistance, low amylose, high amylopectin, and low in vitro digestibility after treatment with AC + E + AC.

PSVI-20 The Effect of Resistant Starch Type II on Satiety Hormones of Fasting Dogs

Abstract Resistant starches comprise the starch fraction that escapes small intestine (SI) digestion and reaches the colon, where it is fermented by saccharolytic bacteria that produce beneficial postbiotics. Moreover, slowly digestible starches lower glycemic index and may promote satiety. The objectives of this study were to assess whether diets having increased RS perturbed fasting levels of satiety hormones in dogs. Diets with three levels of resistant starch (RS type II) were produced via extrusion at three levels of mechanical energy. Nine male and 15 female adult Beagle dogs were fed the low, medium and high RS foods in a 3x3 Latin square design (n = 24). The study was approved by IACUC at Hill’s Pet Nutrition (# 883.0.0.0). Fasting blood was collected in the morning on days 27 and 28 of each period, and serum was separated and frozen at -70°C until analysis. Satiety hormones were measured by ELISA. Data was first converted to natural log to approximate normal distribution and then analyzed as a mixed model by the GLIMMIX procedure from SAS (v 9.4) with diet as fixed effect and dog and period as random effects. Ghrelin, gastric inhibitory polypeptide (GIP), glucagon-like peptide 1 (GLP-1), glucagon, leptin, pancreatic polypeptide (PP) and peptide YY (PYY) were not different across treatments likely due to the fasting state of dogs. Surprisingly, only insulin increased with higher RS consumption. It is possible that more propionate (not measured) was produced in the colon of dogs fed the high RS diet, which went through gluconeogenesis in the liver and resulted in a change in the time of glucose entering the blood stream. This could explain the insulin increase during fasting, but needs to be verified by measurement of fecal SCFA. Future research should explore the effect of type II RS on satiety hormones at multiple time points postprandial.

INDEKS GLIKEMIK COOKIES SORGUM (SORGHUM BICOLOR) DENGAN PENAMBAHAN TEPUNG KACANG MERAH (PHASEOLUS VULGARIS) PRATANAK

Background: The prevalence of diabetes is increasing, one of the pillars of intervention is the provision of high-fiber or complex carbohydrates food. Sorghum as local food has the potential to manufacture as cookies diabetes because it contains fiber and low glycemic index. Foods with a low glycemic index help to prevent against high blood glucose fluctuation. Levels of resistant starch can be improved with the addition of parboiled red beans fluor. Parboiled process aims to improve the digestibility and resistant starch level of red beans. Objective: Determine parboiled red beans fluor % addition which still preferred by consumer panelists, and then determine the glycemic index of cookies sorghum with the addition of selected parboiled red beans fluor. Methods: Experimental study, the first stage was organoleptic test with randomized block design. 6 treatments of parboiled red bean flour and three replications. The addition consists of 0, 10, 20, 30, 40, and 50%. The second stage was the determination of the glycemic index. The subject had to fasting for 10 hours, consumed white bread, and then measured the glycemic response at 0, 30, 60, 90, 120 minute. Four days later the same step repeated with cookies sorghum as food had to consumed. Glycemic index values determined by comparing the inceremental area under curve of cookies sorghum with the incremental area under curve of white bread. Results: The addition of parboiled red bean flour preference is 30%. Cookies sorgum glycemic index was 48.5Conclusion: Cookies sorghum can be used as a snack food for healthy people because it has a low glycemic index

A comparative study on nutritive peculiarities of 24 Chinese cowpea cultivars

The nutritive peculiarities and original distribution of 24 Chinese cowpeas cultivars, achieved from Jiangxi, Jilin, Shanxi and Shaanxi provinces in China, were evaluated. Their nutritional components were determined and nutrient characteristics were discussed. Principal component analysis (PCA) was applied to classify the cowpea samples based on their nutrient composition. The results discovered that the proximate composition varied among 24 cowpeas in protein (17.30–27.23%), fat (1.87–3.14%), high level of dietary fiber (29.49–38.54%) and resistant starch (3.18–31.33%). The total tocopherol varied from 74.20 to 187.07 μg/g. PCA results presented that the 24 cowpeas cultivars were automatically grouped with provinces of origin, indicating a relationship between nutrient composition and cultivating religion for cowpeas. The results of this study provide us the information on the nutrients characteristic of current cowpeas in China, and give a guideline on cowpea cultivating optimization and processing.

PEMBUATAN RESISTANT STARCH PATI BERAS DENGAN METODE ENZIMATIS DAN FISIK

Rice is the leading food and a carbohydrate source for most of the world's and Indonesia population, but most rice tends to have a high glycemic index (IG). A product with a low IG is better, not only for people with diabetes but also for healthy people. Resistant starch (RS) functions as a new food ingredient that has a low glycemic index. RS is a part of starch or starch products resistant to digestion when through a gastrointestinal tract. RS has the same physiological effects as dietary fiber, can affect body weight and energy balance, increase lipid excretion to reduce calorie intake and reduce serum lipid levels, zero caloric content so that it can be used as a low-calorie food additive that can control body weight effectively. RS can be made through starch modification by physical treatment (heat treatment), chemical treatment, enzymatic treatment, and a combination of heat and enzymatic. Factors that influence RS's formation include starch properties, starch interactions with other components, processing conditions, heat processes, other treatments, and storage conditions. Therefore, the rice starch modification that contains a lot of RS needs to be known. Rice starch Hydrolysis with the dual enzyme α-amylase and pullulanase (DMT) can increase the highest RS level (51.0%) due to the increased degree of starch crystallinity. High RS results in an increase in gelatinization temperature, ΔH and phase transition temperatures, and a decrease in the speed of starch hydrolysis and weight loss.

Properties of extruded cross-linked waxy maize starches and their effects on extruded oat flour

The objectives of this study were to study the extrusion of cross-linked waxy maize starches (CLWMS) with different cross-linking levels and their function as a secondary ingredient in extruded oat flour (OF) formulations. CLWMS (18 %) and OF (82 %) were hydrated to 20 % moisture content and subjected to twin-screw extrusion at the screw speed of 350 rpm. Low cross-linking level of CLWMS (0.05 % sodium trimetaphosphate/sodium tripolyphosphate) in OF formulation increased the void fraction and reduced the breaking strength of extrudates. The low cross-linked starch was more resistant to breakdown and had a higher pasting viscosity than the unmodified starch. Higher cross-linking levels of CLWMS restricted swelling of starch granule and increased the resistant starch level of OF formulation but had very poor structural and textural properties. Varying the level of cross-linking offers an alternative way to manipulate the structural, textural and nutritional properties of extrudates in snack and cereal applications.

Biofortification of multi-grain substrates by probiotic yeast

ABSTRACT In the current study, probiotic yeast strain, Saccharomyces cerevisiae AKP1 was assessed for its potential as a starter culture in multi-grain (rice, pulses, and soybean, 3:1:1) substrates fermentation. The impact of fermentation of multi-grain-based food on proximate composition, antinutrients, and antioxidants was evaluated. Fermented product showed significant increments (P <.05) in protein (13.6%) and fiber (1.8%) content. Moreover, the rapidly digestible starch (27.5%) and resistant starch (15.0%) levels were found to increase significantly (P <.05) while the slowly digestible starch level decreased (87.7%) in the fermented food sample. After 4 days of fermentation, total phenolic and total flavonoid contents increased by 83.0% and 69.8%, respectively, with a greater antioxidant potential of 85.9%. The fermented food sample showed a significant reduction in the phytate (64.5%) and trypsin inhibitor activity (19.9%) (P <.05) with a substantial increase in phytase level (P <.05). Fourier transform infrared spectroscopy clearly revealed the alteration of physico-chemical properties during fermentation with S. cerevisiae AKP1. Gas chromatography–mass spectrometry analysis detected the presence of 38 volatile compounds in the fermented food material with the prevalence of fatty acids such as palmitic acid, linoleic acid, among others; alcohols such as isoamyl alcohol, 2,3-butanediol, among others; and esters such as ethyl-2-methylbutanoate. Thus, probiotic yeast S. cerevisiae AKP1 could improve the dietary and functional characteristics of multi-grain substrates and could be regarded as a potential starter for multi-grain substrates fermentation.

Improving quality: Modified celluloses applied to bread dough with high level of resistant starch

Additives have been used in the baking industry for a long time with many purposes, and their use is frequently focused on correcting the quality deficiency of wheat flour. Nevertheless, with the flourishing search for novel functional baked goods, additives have seen expanded their action field since they can exert a quality rectification effect also when novel ingredients are assessed to obtain new functional foods. In this work, two modified celluloses (MC), hydroxypropylmethylcellulose and carboxymethylcellulose, were evaluated as additives for the recovering of rheological and microstructural quality of bread dough enriched with 30% type 2 resistant maize starch (flour basis). The results showed that both MC effectively improved the technological quality of the dough by enhancing the farinographic performance and reducing the high storage modulus of the resistant starch dough without additives. Moreover, only slight modifications were found over the loss modulus and the pasting properties. The observation of the gluten network by CLSM showed that the addition of the MC leads to a more cross-linked and complex gluten network which would explain the longer stability values of the MC-added dough samples found in the farinographic tests. This positive effect would be explained by hydrocolloid-gluten protein interactions as well as by the higher amount of water needed for the preparation of the dough. Finally, the work showed that the use of MC would be a suitable option to get rheology-balanced bread dough that leads to a highly fiber-enriched and good-quality bread.

Baking Effect on Resistant Starch Digestion from Composite Bread Produced with Partial Wheat Flour Substitution

The consumption of composite flour, such as green banana and corn flour, is related to maintain stable blood glucose levels, due to high resistant starch levels. However, most of these studies have conducted analyses of unprocessed food such as flour. Therefore, this study aimed to evaluate the effect of baking on resistant starch concentration and digestion from bread produced with partial wheat flour substitution. Response surface methodology was used to evaluate bread physical-chemical characteristics, and then, sensorial and nutritional qualities of the bread were evaluated. The feasibility of incorporating 40% of corn flour was demonstrated, while incorporation of 20% produced bread with similar characteristics to the control; for green banana flour, these levels were 20 and 10%, respectively. Resistant starch levels of composite breads were also enhanced by in vitro analyses. On the other hand, in vivo blood glucose levels evidenced that the ingestion of breads produced with partial wheat flour substitution by green banana or corn flour promoted a more important peak in blood glucose levels in comparison with control bread, which was never previously presented in the literature. Bread ingestion rapidly increased the blood glucose levels of rats; once during the baking process, starch granules become gelatinized and therefore easily digestible. Furthermore, this study also highlighted the lack and need for future investigation of wheat flour-substituted baked goods, in order to better understand mechanical properties formation and also product digestibility.

Development and evaluation of chapatti quality of high amylose wheat mutants on the basis of physicochemical, textural and sensory characteristics

Considerable endeavours have been made to develop improved wheat varieties. In fact, starch with increased level of amylose or resistant starch is one of the key research areas that benefits human health. In this study eight high amylose wheat mutant lines including their parent and market flours were assessed for chapatti quality characteristics on the basis of physicochemical (DSC, XRD, RVA), textural, and sensory parameters. Amylose content of mutant lines ranged from ~51 to 75%. Mutant DSC analysis of starch showed variation with increased gelatinization. RVA, XRD, and mixograph data showed variation in starch pasting, crystallinity, and dough characteristics among mutants and parents. 37 other parameters related to grain, starch, flour, dough and chapatti quality were analysed using principal component analysis, ANOVA, and correlation coefficients. Three principal components (PC1, PC2, and PC3) accounted for 68% of the total variation. Strong correlation was observed between amylose and resistant starch content (r2= 0.87). Baking time first side (BTFS) had negative impact on eating quality (EQ) (r2 = −0.81) and pliability was positively correlated to softness (r2 = 0.99). Except for mutant ‘TAC 75’ overall quality scores (49.5–55.5) were as good as or better than parent variety, ‘C 306’ (49.62).

Interactive effects between sugar source and pelleting temperature on processing, digestibility and blood metabolites in nursery piglets

The aim of this study was to evaluate the effect of pelleting temperature (60°C or 90°C) and the type of sugar source (refined sugar cane or sugar cane molasses), on post-processed diet characteristics and on animal body weight gain, apparent total tract digestibility of energy and nutrient, and blood metabolites. Thirty-two recently-weaned piglets of both sexes were randomly assigned to 1 of the 4 treatments in a 2 (sugar source, refined sugar cane or sugar cane molasses) x 2 (pelleting temperature, 60°C or 90°C) factorial arrangement. The physicochemical characteristics of the diets evaluated were pellet durability index, fine content and starch gelatinization index. After seven days of adaptation, partial fecal samples were collected for apparent digestibility analyses for five days. For weight gain evaluation, the animals were weighed at 28, 35, 40 and 63 days of age. Blood samples were collected at the beginning (28 days) and the end of the experiment (63 days). Data were submitted to variance analysis and the means were compared by the Tukey test. There was interaction between pelleting temperature and sugar source on the pellet durability index, on the fine content and on the starch gelatinization index (P < 0.05) of the diets. Pelletizing at 90°C increased the pellet durability index in treatment with sugar cane molasses. The fine content was reduced in treatments with pelleting temperature at 90°C and increased in treatments with sugar cane molasses. There was an increase of the starch gelatinization index with refined sugar cane at 90°C. There was no interaction between pelleting temperature and sugar source on starch composition of the diets. The pelleting temperature and the type of sugar source did not influence the levels of resistant and non-resistant starch (P > 0.05). The total starch index was higher in treatments using sugar cane molasses. There was no interaction between pelleting temperature and sugar source on nutrient apparent total tract digestibility. The use of sugar cane molasses promoted higher apparent digestibility of gross energy and ash (A), and pelleting at 90°C increased the apparent digestibility of Ca, P and A (P < 0.05). There was interaction between pelleting temperature and sugar source on the gamma-glutamyltransferase (GGT) enzyme (P < 0.05), where the use of sugar cane molasses reduced GGT levels in the pelleted diet at 60°C. There was no effect of pelleting temperature and the type of sugar source on animal body weight gain, high density cholesterol (HDL), total cholesterol, triglycerides, urea and albumin. Therefore, sugar cane molasses can replace refined sugar cane as the palatable ingredient in the diet of nursery piglets without affecting animal body weight gain and levels of blood metabolites of the animals, promoting greater apparent digestibility of gross energy and ash, although it had altered the physicochemical characteristics of the diet. The use of sugar cane molasses allowed for the exploration of higher pelleting temperatures (90°C) in the feed processing, leading to increasing the apparent digestibility of Ca, P and A of the diets.