Recovery Of Levels Research Articles

Monitoring the heart rate variability of servicewomen of the Armed Forces of Ukraine during the legal regime of martial law

The article features the results of the analysis of heart rate variability of 30 female military personnel (n=30). The purpose of this study was to assess the state of regulatory mechanisms of the autonomic nervous system. The analysis of heart rate variability indicators reflects the general level of autonomic regulation, which is important for assessing the physical and psychological state of military personnel. The RRNN parameter had a median of 814.5 ms, which corresponds to the norm for healthy individuals and indicates a normal level of cardiac activity of females being under the research. The SDNN indicator, which characterizes the total effect of autonomic regulation of blood circulation, was 37.4 ms on the median, which also corresponds to the norm and indicates an adequate level of adaptation capabilities of the body. However, RMSSD was at the median below normal, indicating reduced recovery and high levels of stress among female military personnel. pNN50, which reflects the proportion of consecutive RR intervals differing by more than 50 ms, was also below normal, further confirming reduced parasympathetic activity. The LF index was 301.0 ms² on the median of female military personnel, which is at the lower limit of the norm. The result we obtained may indicate a reduced sympathetic influence or a general depletion of adaptation reserves. The VLF indicator, which characterizes neurohumoral regulation, was also below the norm, which indicates a possible depletion of the body's adaptive reserves. Such studies make it possible to detect signs of fatigue or insufficient recovery in time and take the necessary measures to optimize the training process and the performance of official duties. Our research confirms that heart rate variability is an important indicator of the body's adaptive capabilities, especially in the conditions of martial law, where stress and physical exertion are constant factors of influence.

Pre-drought effects on northern temperate trees and vine invasion in forest gaps hindering regeneration

Northern temperate coniferous forests serve as crucial connectors between boreal and temperate forests, yet they are vulnerable to various stressors such as climate change and human activities. Severe drought poses a significant threat to plant species within these forests, prompting recent research into its impacts. However, many studies lack explicit definitions of post-disturbance vegetation processes and fail to identify potential interactions with disturbance factors, necessitating comprehensive discussions. This study examines the effects of drought on tree growth patterns of the main dominant species in northern temperate regions: Abies nephrolepis and Picea jezoensis, along with two commonly associated Betula ermanii, and Quercus mongolica. Additionally, new disturbance factors in forests inhabited by these species (A. nephrolepis and P. jezoensis) were evaluated based on community classification. The study sites were located in the Mt. Baekdu (Changbai) and South Korea regions, which are positioned at the southern limit of the phytogeographical patterns of target species. Results indicate that A. nephrolepis and P. jezoensis exhibit high levels of recovery and resilience, while B. ermanii and Q. mongolica demonstrate high resistance. Species-specific responses align with drought intensity, with resistance, recovery, and resilience decreasing notably with increasing pre-drought radial growth. South Korean forests, the invasion of the vine species Tripterygium regelii after the death of A. nephrolepis in the overstory vegetation threatens the regeneration of new trees. However, certain environmental factors, such as high rock exposure and dense overstory canopy, limit vine invasion. Based on the results, pre-drought radial growth emerges as a key determinant in how trees respond to drought. Additionally, the results suggest the potential for new disturbances to emerge in forest gaps due to overstory vegetation mortality induced by global warming. These findings contribute to a deeper understanding of increasing drought stress, aid in identifying climate refugia, and inform conservation priorities based on habitat characteristics.

Lipid loss and compositional change during preparation of simple two-component liposomes

Liposomes are used as model membranes in many scientific fields. Various methods exist to prepare liposomes, but common procedures include thin-film hydration followed by extrusion, freeze-thaw, and/or sonication. These procedures can produce liposomes at specific concentrations and lipid compositions, and researchers often assume that the concentration and composition of their liposomes are similar or identical to what would be expected if no lipid loss occurred. However, lipid loss and concomitant biasing of lipid composition can in principle occur at any preparation step due to nonideal mixing, lipid-surface interactions, etc. Here, we report a straightforward HPLC-ELSD method to quantify the lipid concentration and composition of liposomes and apply that method to study the preparation of simple cholesterol/POPC liposomes. We examine common liposome preparation steps, including vortexing during resuspension, lipid film hydration, extrusion, freeze-thaw, and sonication. We found that the resuspension step can play an outsized role in determining the lipid loss (up to ∼50% under seemingly rigorous procedures). The extrusion step yielded smaller lipid losses (∼10–20%). Freeze-thaw and sonication could both be employed to improve lipid yields. Hydration times up to 60 min and increasing cholesterol concentrations up to 50 mol % had little influence on lipid recovery. Fortunately, even conditions with large lipid loss did not substantially influence the target membrane composition, as long as the lipid mixture was below the cholesterol solubility limit. From our results, we identify best practices for producing maximum levels of lipid recovery and minimal changes to lipid composition during liposome preparation for cholesterol/POPC liposomes.

Impact of the COVID-19 pandemic on electronic referrals to rapid access clinics for suspected breast, lung and prostate cancers in Ireland.

The coronavirus disease 2019 (COVID-19) pandemic impacted cancer services worldwide. We examined the effect of the first three pandemic waves on the number of electronic (e)-referrals to rapid access clinics (RACs) for breast, lung and prostate cancer in Ireland. This study used a retrospective, repeated cross-sectional design. The predicted weekly number of e-referrals by suspected cancer types from March 2020 to May 2021 was calculated using the Holt-Winters seasonal smoothing method, based on the observed numbers from a representative pre-pandemic period (01 January 2019 to 01 March 2020) and compared this with the observed number across the first three pandemic waves (02 March 2020 to 09 May 2021). Percentage differences were calculated between observed and predicted numbers of e-referrals for the three RACs and patterns were examined in each wave. Observed e-referrals were lower than predicted for all three RACs in the first wave of the pandemic (15.7% lower for breast, 39.5% lower for lung and 28.1% lower for prostate) with varying levels of recovery in the second and third waves for the three e-referral types. The COVID-19 pandemic impacted patterns of e-referrals to RACs in the first three pandemic waves in Ireland. Early identification of changes in engagement with health services, such as a decrease in primary care presentations with a resultant decrease in e-referrals to RACs can allow for a rapid response from cancer control programmes. Continued surveillance of the impact of service disruption on cancer services allows policy makers and strategic leaders in cancer control programmes to respond rapidly to mitigate the impact on cancer outcomes.

Characterization of rhodanese synthesized by the wild and EMS-mutated Klebsiella oxytoca JCM1665

Cyanide poisoning remains a significant concern for both civilian and military personnel worldwide. Rhodanese, a cyanide detoxify enzyme produced by the wild (KOJCM1665) and two selected mutant strains (KOJCM1665c and KOJCM1665d) of Klebsiella oxytoca JCM 1665 was purified and characterized using a combination of standard techniques. Extracellular rhodanese yield of the wild and selected mutants were 6.2 ± 0.6, 26.7 ± 0.7 and 18.3 ± 0.3 U/mL respectively. The overall levels of recovery of rhodanese activity and fold after purification were 49 %, 66 %, and 58 % and 2.6, 2.6, and 2.3 % for the wild, KOJCM 1665c, and KOJCM 1665d, respectively. The native molecular weights of the three enzyme preparations were 35.1, 34.9, and 34.8 kDa, while the subunit molecular weight was 35 kDa for all the enzymes. The optimum activity of the enzymes was observed at 50 °C and pH 6.0. The real km of the three enzyme preparations for KCN as a substrate were 1.03 × 10−3, 0.95 × 10−3, and 0.80 × 10−3 M, respectively, while the km for the second substrate (Na2S2O3) were 0.59 × 10−3, 0.49 × 10−3, and 0.69 × 10−3 M, respectively. The substrate specificity study showed that the enzyme preferred sodium thiosulfate as the substrate. Metal ions such as Na+ and K+ had significantly greater inhibitory effects on enzyme activity. This study demonstrated the potential of enhancing K. oxytoca JCM 1665 to overexpress extracellular rhodanese, and the physicochemical properties of the enzyme hold significant promise as a target for improving the efficiency of cyanide bioremediation processes.

Plastic deformation and recovery in ultrathin aluminosilicate glass

Glass was generally considered to be brittle, and its applications were significantly limited by its vulnerability to fracture caused by deformation. The folding tests with ultrathin glass (UTG) conducted in this work illustrate that glass can also deform plastically and generate permanent creases on a macroscopic level. Moreover, the plastic deformation can gradually and partially recover at room temperature and the level of recovery can be inhibited by a longer holding time or through repeated loading. Based on the experimental observation, a phenomenological model is established to predict the plastic behavior of the concerned glass and we further discuss the possible cause of plastic deformation and its recovery and the potential applications.

Assessment of Parental Risk Factors Involved in Orofacial Clefts in Hail, Saudi Arabia: A Retrospective Cohort Study.

Background Orofacial clefts are congenital anomalies affecting the development of the oral and facial structures, influenced by genetic and environmental factors. The prevalence of orofacial clefts varies globally, necessitating region-specific studies to understand contributing factors.Orofacial clefts are among the most common congenital defects affecting the head and neck, underscoring the importance of investigating paternal and maternal influences on their development to enhance awareness and understanding of potential contributing factors.Therefore, this research aimed to investigate parental risk factors contributing to the development of orofacial clefts. Methods A retrospective cohort study was conducted at the Oral and Maxillofacial Department of King Khaled Hospital,Hail, Saudi Arabia, involving 40 parents of children born between 2019 and 2023 with orofacial clefts. Data collection included interviewer-administered questionnaires with parents addressing demographic information, pregnancy details, parental medical history, and postoperative outcomes. Statistical analysis utilized descriptive statistics, chi-square tests, Fisher's exact test, and linear regression, with significance defined as p<0.05. Results The study had a gender distribution of 19 males (47.5%) and 21 females (52.5%) among orofacial cleft cases (p<0.75), with cleft palate (13 cases, 32.5%) and cleft lip (11 cases, 27.5%) being the most prevalent anomalies (p<0.05). Maternal supplementation rates were high, with 34 mothers (85%, p<0.05) taking folic acid and 36 mothers (90%, p<0.05) taking iron, yet orofacial clefts incidence persisted. Paternal risk factors such as tobacco use were reported by 19 fathers (47.5%, p<0.05), and familial history of orofacial clefts was noted in nine cases (22.5%, p<0.05). Postoperative outcomes indicated varying levels of functional recovery and satisfaction. Conclusion This study explored the complex origins of orofacial clefts, emphasizing genetic and environmental influences. The findings suggest a potential paternal risk factor. The study highlights the need for further investigation into genetic mechanisms and the development of effective prevention strategies.

A novel urease-assisted ratiometric fluorescence sensing platform based on pH-modulated copper-quenched near-infrared carbon dots and methyl red-quenched red carbon dots for selective urea monitoring.

A novel and sensitive fluorescence ratiometric method is developed for urea detection based on the pH-sensitive response of two fluorescent carbon dot (CD) systems: R-CDs/methyl red (MR) and NIR-CDs/Cu2+. The sensing mechanism involves breaking down urea using the enzyme urease, releasing ammonia and increasing pH. At higher pH, the fluorescence of NIR-CDs is quenched due to the enhanced interaction with Cu2+, while the fluorescence of R-CDs is restored as the acidic MR converts to its basic form, removing the inner filter effect. The ratiometric signal (F608/F750) of the R-CDs/MR and NIR-CDs/Cu2+ intensities changed in response to the pH induced by urea hydrolysis, enabling selective and sensitive urea detection. Detailed spectroscopic and morphological investigations confirmed the fluorescence probe design and elucidated the sensing mechanism. The method exhibited excellent sensitivity (0.00028 mMLOD) and linearity range (0.001 - 8.0 mM) for urea detection, with successful application in milk samples for monitoring adulteration, demonstrating negligible interference and high recovery levels (96.5% to 101.0%). This ratiometric fluorescence approach offers a robust strategy for selective urea sensing in complicated matrices.

Marker-assisted introgression to improve the oleic acid content in the TMV 7 groundnut (Arachis hypogaea L.) variety suitable for the oil industry

BackgroundImproving the quality and shelf life of groundnut oil is one of the foremost objectives of groundnut breeding programmes. This can be achieved by marker-assisted introgression, a technique that efficiently and precisely enables breeders to develop plants with enhanced qualities. This study focused on improving the oleic acid content of an elite groundnut variety, TMV 7, by introgressing a recessive mutation responsible for the increase in oleic acid from ICG 15419. Hybridization was performed between the donor and recurrent parents to develop the F1, BC1F1, BC2F1 and BC2F2 populations. Introgressed lines with increased oleic acid in the genetic background of TMV 7 were identified using allele-specific marker, F435-F, F435SUB-R and a set of SSR markers were employed to recover the genome of the recurrent parent. ResultsWith two backcrosses, a total of ten homozygous plants in the BC2F2 population were identified with oleic acid content ranging from 54.23 to 57.72% causing an increase of 36% over the recurrent parent. Among the ten lines, the line IL-23 exhibited the highest level of recurrent parent genome recovery of 91.12%.ConclusionsThe phenotypic evaluation of 10 homozygous introgressed lines indicated fewer differences for all other traits under study compared to the recurrent parent, except for oleic acid and linoleic acid content confirming the genetic background of the recurrent parent. The identified lines will be subjected to multilocation trials before their commercial release.

Reliability model and emergency maintenance strategies for smart home systems

After the smart home system is impacted by malicious attacks or other factors, the system reliability drops drastically, so the user's living environment is greatly disturbed. To determine the emergency maintenance strategy, firstly, we study the failure mechanism of the smart home system by the fault tree theory, and distinguish the components into two types: critical components and non-critical components. Secondly, a recovery prioritization is proposed to measure the extent to which changes in the reliability of each component affect the reliability of the system. Then, three scenarios of smart home system failure are proposed. Under the constraints of time and cost, the maintenance strategies for the three scenarios are determined to achieve emergency recovery. Finally, through the multi-objective particle swarm optimization algorithm, the system reliability is maximized, the maintenance cost is minimized, and the optimal reliability recovery level of each component is obtained, so that the reliability of the smart home system is further improved. The effectiveness and practicality of the emergency maintenance strategies proposed in this paper are demonstrated by a smart home system example.

Waste heat recovery and valorization to electricity and steam: Use of Heat2Power® tool in a real case study from the steel industry

Waste heat recovery (WHR) in steel shops and valorization (WHV) to electricity and steam can considerably cut off the environmental burden of the steelmaking process. However, WHR/WHV is a complex issue, as there are multiple, interrelated, parameters that needs quantifying at recovery and transfer levels. In this context, we developed and launched an optimization tool that is based on mixed-integer linear programming, commercially called Heat2Power®, to provide customized WHR/WHV solutions that strictly respect all the user's objectives. For instance, the user can define the waste heat streams availability over the examined time period, the waste heat valorization path (electricity or steam production or both) and selection of the thermodynamic cycle or turbine technology to be used (inputs). The Heat2Powe® tool can be used either as a customized process calculator calculating the economics and the volumetric footprint of the WHR/WHV unit (output 1), should the user define all the system parameters or as a process optimizer, should the user not be able to specify all the design parameters, leaving the optimizer to select the most efficient WHR/WHV technology for a given operating window (output 2). Comparing the Heat2Power® results with data retrieved from a real WHR/WHV case of the steel industry, it is found that a difference of less than 20 % is attained when identical operating conditions are considered, rendering Heat2Power® a trustful and valuable decision-making tool, especially for projects being at the design and development phase.

Acute inorganic nitrate ingestion does not impact oral microbial composition, cognitive function, or high-intensity exercise performance in female team-sport athletes.

The purpose of this study was to investigate the effects of acute nitrate (NO3-)-rich beetroot juice ingestion on explosive and high-intensity exercise performance, oral microbiota composition, and cognitive flexibility (i.e., function), before and after maximal intermittent running exercise. Fifteen women team-sport athletes were assigned in a randomized, double-blind, crossover design to consume concentrated NO3--depleted beetroot juice (PL; 0.1mmol NO3-) and NO3--rich beetroot juice (BR; 12.0mmol NO3-) 2.5h prior to performing a battery of exercise performance tasks and cognitive testing before and after the Yo-Yo intermittent recovery level 1 (YYIR1) running test. Resting plasma [NO3-] and plasma nitrite ([NO2-]) were elevated following BR (P < 0.001). BR did not impact global composition or relative abundance of taxa in the oral microbiome (P > 0.05) or cognitive flexibility before or after exercise (P > 0.05). There was no significant difference in performance during 20-m (PRE, PL: 4.38 ± 0.27 vs. BR: 4.38 ± 0.32s; POST, PL: 4.45 ± 0.29 vs. BR: 4.43 ± 0.35s) and 10-m sprints (PRE, PL 2.78 ± 0.15 vs. BR 2.79 ± 0.18s; POST, PL: 2.82 ± 0.16 vs. BR: 2.81 ± 0.19s), isokinetic handgrip dynamometry, medicine ball throw, horizontal countermovement jump, or YYIR1 (PL: 355 ± 163m vs. BR: 368 ± 184m) between BR and PL (P > 0.05). These findings indicate that acute dietary NO3- may not influence the oral microbiome, explosive and high-intensity exercise performance, or cognitive function in women team-sport athletes.

The role of personal recovery and internalised stigma on the expression of symptomatology in severe mental disorders: Mediating and moderating effects

ObjectiveGiven the relevance of internalised stigma in people suffering from a mental disorder, in the present study, the possible mediating and moderating role of self-stigma in the relationship between personal recovery and symptomatology has been studied. Method265 participants with severe mental disorder completed the following instruments: ISMI (self-stigma), REE (personal recovery) and HoNOS, CGI, GAF and EuroQol (symptomatology). Resultsboth the mediation and moderation analyses show significant results, which would indicate that internalised stigma has an effect on the relationship between personal recovery and symptomatology. Also, people with lower level of personal recovery and greater self-stigma have greater symptomatology than those who are in more advanced personal recovery processes and have a lower perception of internalised stigma. Discussionthe findings of this study suggest that self-stigma has an effect, and the improvement at personal recovery and symptomatology is accentuated when people with a severe mental disorder have a better management of internalised stigma. Therefore, it may be interesting to include this variable in recovery interventions.

THE USE OF SUBCISION IN CONJUNCTION WITH FRACTIONAL CARBON DIOXIDE LASER TO IMPROVE ATROPHIC ACNE SCARS

Objective: Acne is a prevalent skin condition affecting a wide range of age groups, often leading to severe scarring if left untreated. The use of subcision combined with fractional CO2 laser therapy has been posited as a potentially more effective treatment method for atrophic acne scars compared to standalone laser treatment. Methods: This clinical trial was conducted at Asha Derma Clinic, Gorakhpur, Uttar Pradesh, from 30th March 2023 to 30th March 2024. Thirty patients with ice pick and rolling-type atrophic acne scars were enrolled. Participants were treated on one side of their face with five sessions of fractional CO2 laser and on the other side with one session of subcision with fractional CO2 laser combined with four sessions of fractional CO2 laser alone. Outcomes were assessed through scar improvement and patient satisfaction at 1, 2, and 6 mo post-treatment, with data analysis performed using ANOVA and the Wilcoxon rank-sum test. Results: Significant improvements were observed in both recovery levels and patient satisfaction. The combined method showed higher recovery percentages (40-65%, Mean±SD: 54.7±7) compared to the laser alone (30-55%, Mean±SD: 43±11.1), with a p-value of 0.0005. Patient satisfaction also favored the combined method (1.8-3, Mean±SD: 6.6±1.2) over laser treatment alone (1.6-4, Mean±SD: 5.2±1.8), with a p-value of 0.0007. Conclusion: The study demonstrated that subcision combined with fractional CO2 laser therapy significantly enhances recovery levels and patient satisfaction in the treatment of atrophic acne scars, suggesting a robust advantage of integrating both treatments.

Clinical efficacy of laparoscopic cholecystectomy combined with endoscopic papillary balloon dilation in treatment of gallbladder stones with common bile duct stones: A retrospective study.

The incidence of cholelithiasis has been on the rise in recent years, but the choice of procedure is controversial. To investigate the efficacy of laparoscopic cholecystectomy (LC) combined with endoscopic papillary balloon dilation (EPBD) in patients with gallbladder stones (GS) with common bile duct stones (CBDS). The clinical data of 102 patients with GS combined with CBDS were selected for retrospective analysis and divided into either an LC + EPBD group (n = 50) or an LC + endoscopic sphincterotomy (EST) group (n = 52) according to surgical methods. Surgery-related indexes, postoperative recovery, postoperative complications, and expression levels of inflammatory response indexes were compared between the two groups. Total surgical time, stone free rate, rate of conversion to laparotomy, and successful stone extraction rate did not differ significantly between the LC + EPBD group and LC + EST group. Intraoperative hemorrhage, time to ambulation, and length of hospitalization in the LC + EPBD group were lower than those of the LC + EST group (P < 0.05). The rate of total complications of the two groups was 9.80% and 17.65%, respectively, and the difference was not statistically significant. No serious complications occurred in either group. At 48 h postoperatively, the expression levels of interleukin-6, tumor necrosis factor-α, high-sensitivity C-reactive protein, and procalcitonin were lower in the LC + EPBD group than in the LC + EST group (P < 0.05). At 3 d postoperatively, the expression levels of aspartate transaminase, alanine transaminase, and total bilirubin were lower in the LC + EPBD group than in the LC + EST group (P < 0.05). LC combined with EPBD and LC combined with EST are both effective procedures for the treatment of GS with CBDS, in which LC combined with EPBD is beneficial to shorten the patient's hospitalization time, reduce the magnitude of elevated inflammatory response indexes, and promote postoperative recovery.

Abdominal wound length influences the postoperative serum level of interleukin-6 and recovery of flatus passage among patients with colorectal cancer.

A mini-laparotomy for colorectal cancer (CRC) has been reported to shorten postoperative ileus (POI) and hospital stay. Interleukin-6 (IL-6) plays a role in intestinal tissue inflammation, leading to POI. This study investigated the effects of abdominal wounds and IL-6 levels on POI in patients having CRC surgery. Forty-three patients with CRC underwent bowel resection. Serum samples were collected preoperatively and at 2, 24, and 48 h after surgery for cytokine quantification by ELISA. Clinical data, including time from surgery to first passage of flatus and postoperative hospital stay, demographic and pathological data, and routine blood tests, were compared statistically with abdominal wound length and the postoperative increments of cytokines (designated as Δ). The length of the abdominal wound showed a significant correlation with clinical variables (length of operation time, time of first flatus passage, and length of postoperative hospital stay) and cytokine variables (IL-6(Δ2 h), IL-8(Δ2 h) and IL-10(Δ2 h). Linear regression analysis showed that the abdominal wound length significantly influenced the operation time, time of first flatus passage, and length of postoperative hospital stay (p < 0.001). The length of the abdominal wound showed a significant influence on the IL-6(Δ2 h) and IL-8(Δ2 h) (p < 0.001, respectively) but no influence on IL-10(Δ2 h). IL-6(Δ2 h), but not IL-8(Δ2 h), significantly influenced the time to first flatus passage and length of hospital stay (p = 0.007, p = 0.006, respectively). The mini-laparotomy approach (wound length <7 cm) led to significantly shortened operation time, time of first flatus passage, length of postoperative stay (p = 0.004, p = 0.003, p = 0.006, respectively) as well as reduced postoperative increment of IL-6(Δ2 h) (p = 0.015). The mini-laparotomy for anterior resection surgery significantly influenced operation time, time of first passage of flatus, length of postoperative stay, and IL-6(Δ2 h). Our study is the first to report the complex interaction among the length of the abdominal wound, IL-6 serum level, recovery of the first passage of flatus, and postoperative hospital stay. These results suggest that smaller abdominal wounds and smaller postoperative IL-6 increments were associated with faster recovery of flatus passage and shorter hospital stays.

Increasing disturbance frequency undermines coral reef recovery

AbstractClimate‐driven alterations to disturbance regimes are increasingly disrupting patterns of recovery in many biomes. Here, we examine the impact of disturbance and subsequent level of recovery in live hard coral cover on the Great Barrier Reef (GBR) across the last three decades. We demonstrate that a preexisting pattern of infrequent disturbances of limited spatial extent has changed to larger and more frequent disturbances, dominated by marine heatwaves and severe tropical cyclones. We detected an increase in the impact (measured as coral loss) across 265 individual disturbance impacts on 131 reefs in a 36‐year dataset (1985–2022). Additionally, the number of survey reefs impacted by disturbance has increased each decade from 6% in the 1980s to 44% in the 2010s, as has the frequency of mass coral bleaching across the GBR, which has increased between 19% and 28% per year, and cyclones (3%–5% per year), resulting in less time for recovery. Of the 265 disturbance impacts we recorded, complete recovery to the highest levels of coral cover recorded earlier in this study (the “historical benchmark”) occurred only 62 (23%) times. Of the 23% of disturbance impacts that resulted in complete recovery to historical benchmarks, 34/62 recovered to their benchmark in 2021 or 2022. Complete recovery was more likely when the historical benchmark was &lt;25% live hard coral cover. The lack of recovery was attributed to recovery time windows becoming shorter due to increases in the frequency of cyclones and of thermal stress events that result in mass coral bleaching episodes. These results confirm that climate change is contributing to ecosystem‐wide changes in the ability of coral reefs to recover.

A Deep Learning Approach for Classification of Physiotherapy Exercises Using Segmentation of Techniques

: Physiotherapy exercises are necessary to patients to restore their functional abilities in many cases as disabilities, injury, or basic with complementary approach as balneotherapy. Different type of exercised and different template sessions are used depending on the medical diagnostics. The evaluation of effectiveness of these exercises are important for patient’s rehabilitation process as time and level of recovery of locomotor skills. A dataset publicly available (Physical Therapy Exercises) is used for classification of session of repeated exercises that includes movement executed correct (C), fast execution (F) and low-amplitude execution (L). A novel approach is proposed by using segmentation of signal using deep learning neural network followed by a convolutional neural network for classification of sequence of the labeled classes L,C, F, and N (a new class introduced to label the noise of sensor of exercised or incorrect movement of the patient. The signal is extensively analyzed in order to made and corresponding labeling for analyzing using sliding window with a drive user selected length. The accuracy of classification is greater than 96% and sensitivity is greater than 95% but the results can be better if the labelling of N class is more restrictive and the effect of imbalanced dataset is reduced. Keywords: physiotherapy exercises; segmentation techniques; deep learning neural networks, classification; imbalanced dataset

Child temperamental negative affectivity moderates the relation between interparental conflict and child cortisol recovery

AbstractThis study examined the relation between interparental conflict and cortisol recovery, with child temperamental negative affectivity as the moderator. Children (n = 118) ages 9–11 years observed an argument between their parents in the lab and provided saliva samples for cortisol assays. Children also reported levels of interparental conflict, and mothers reported their child's negative affectivity. Results showed that youths with high levels of negative affectivity experienced less cortisol recovery as interparental conflict levels increased. Further, demonstrating some support for differential susceptibility, youths with higher levels of negative affectivity exhibited less recovery at high levels of parental conflict but greater recovery at low levels of parental conflict, compared to peers with lower scores on negative affectivity. We also found that children with low levels of negative affectivity exhibited greater recovery as rates of interparental conflict increased. In summary, this study sheds light on sources of variability in cortisol recovery as it relates to interparental disharmony.

Improved efficiency of ion trapping time-of-flight mass spectrometry for the analysis of pesticide residues and mycotoxins at trace levels in baby food

The analysis of pesticide residues and mycotoxins in baby food demands exceptionally low limits of quantitation, necessitating the use of highly sensitive instruments capable of conducting trace analyses. High-resolution instruments typically fail to detect such low levels. However, the latest advancements in liquid time-of-flight technology, when coupled with ion trapping, enable ion enrichment, thereby improving detection levels. This allows for the analysis of these substances at low concentration levels, benefiting from enhanced mass accuracy. Additionally, the use of mass accuracy data helped eliminate matrix interferences, thereby enabling high-confidence identification. We developed a multi-residue method to analyse 219 pesticide residues and 9 mycotoxin residues in baby food matrices. Utilizing a QuEChERS-based extraction method, the samples were then analysed using an LC-ZenoTOF 7600 system with mass window screening acquisition. For pesticides, the limit of quantitation was 0.001–0.003 mg/kg for 81 % of the evaluated compounds, 0.005 mg/kg for 13 %, 0.010 mg/kg for 4 % and 0.020–0.030 for 2 %; good linearities were obtained at these levels. Apparent recoveries were evaluated at 0.003, 0.005, and 0.010 mg/kg. At the lowest recovery level, 93 % of compounds showed recoveries between 70 and 120 %. The rest of the compounds were in the range of 63–129 %, with relative standard deviation values below 20 %. For mycotoxins, the limits of quantitation ranged from 0.0001 to 0.100 mg/kg, with matrix-matched concentrations assessed within this range. Recoveries were evaluated at low concentration range (0.001–0.003 mg/kg) and high range (0.020–0.050) with apparent recoveries values between 92 and 140 %. Finally, a total of 31 commercial baby food samples were analysed using this method. The results indicated that 16 samples contained pesticide residues, while two samples were found to have mycotoxins.