Nitrogen Levels Research Articles

A pilot clinical risk model to predict polymyxin-induced nephrotoxicity: a real-world, retrospective cohort study.

Polymyxin-induced nephrotoxicity (PIN) is a major safety concern and challenge in clinical practice, which limits the clinical use of polymyxins. This study aims to investigate the risk factors and to develop a scoring tool for the early prediction of PIN. Data on critically ill patients who received intravenous polymyxin B or colistin sulfate for over 24 h were collected. Logistic regression with the least absolute shrinkage and selection operator (LASSO) was used to identify variables that are associated with outcomes. The eXtreme Gradient Boosting (XGB) classifier algorithm was used to further visualize factors with significant differences. A prediction model for PIN was developed through binary logistic regression analysis and the model was assessed by temporal validation and external validation. Finally, a risk-scoring system was developed based on the prediction model. Of 508 patients, 161 (31.6%) patients developed PIN. Polymyxin type, loading dose, septic shock, concomitant vasopressors and baseline blood urea nitrogen (BUN) level were identified as significant predictors of PIN. All validation exhibited great discrimination, with the AUC of 0.742 (95% CI: 0.696-0.787) for internal validation, of 0.708 (95% CI: 0.605-0.810) for temporal validation and of 0.874 (95% CI: 0.759-0.989) for external validation, respectively. A simple risk-scoring tool was developed with a total risk score ranging from -3 to 4, corresponding to a risk of PIN from 0.79% to 81.24%. This study established a prediction model for PIN. Before using polymyxins, the simple risk-scoring tool can effectively identify patients at risk of developing PIN within a range of 7% to 65%.

Field study on the integration of a hydrogen-producing microbiome in restoring Phellinus noxius-affected rhizosphere

At National Kaohsiung University of Science and Technology (NKUST), a mesophilic anaerobic bioreactor system produces a hydrogen-producing microbiome (HMb) containing beneficial bacteria such as Bacillus sp. This HMb, known for degrading cellulose and remediating soil, is being tested in Kaohsiung City, Taiwan, to treat tree root rot caused by Phellinus noxius (P. noxius). The study aims to evaluate HMb's effectiveness in restoring the rhizosphere, enhancing soil health, and increasing tree resistance by assessing soil bioactivity and microbial diversity.Field experiments showed that HMb treatment improved tree health by 24 % at the highest health level but reduced it by 20 % at the next level. Post-treatment soil organic matter (SOM) and total Kjeldahl nitrogen (TKN) levels remained stable, indicating minimal impact on soil fertility. HMb also lowered soil pH in some plots, potentially suppressing pathogens and enhancing nutrient absorption. The study found that HMb increased microbial diversity in the rhizosphere, particularly Acidobacteria and Proteobacteria, which inhibited fungal growth. However, HMb had no significant impact on P. noxius populations. Overall, HMb treatment shows promise in enhancing plant health by improving microbial diversity and soil conditions, but further research is needed to understand its full impact on pathogen suppression and soil health restoration.

Nephroprotective effect of sugarcane (Saccharum officinarum L.) leaves ethanol extract on gentamicin-induced nephrotoxicity in rats

Kidney damage is commonly attributed to using certain drugs, such as gentamicin, which causes elevated kidney parameters in blood and damage to renal tissue. This damage is often a result of oxidative stress, but it can be mitigated by using antioxidants. Several studies proved the potential of sugarcane (Saccharum officinarum L.) leaves as an antioxidant. Therefore, this experiment aimed to examine the nephroprotective action of sugarcane leaves. Twenty-five Wistar rats were separated into the normal, negative, and sugarcane leaf extract (SLE) (200, 400, and 600 mg/kg BW) groups. The animals were handled for 8 days, and then, the blood and tissue were collected 24 h later. The results revealed that SLE prevents increased creatinine, blood urea nitrogen, uric acid, and malondialdehyde levels. The histology analysis indicated that the extract improved kidney morphology and histopathology. Sugarcane leaves have the potential to be a nephroprotective agent.

Assessing the impact of rainfall on water quality in a coastal urban river utilizing the environmental fluid dynamics code

The Haihe River, flowing through Tianjin City, grapples with pollution challenges, notably high levels of nitrogen (N) and phosphorus (P) leading to eutrophication and unpleasant odors, particularly aggravated by rainfall. This study used Environmental Fluid Dynamics Code to comprehensively analyze the water environment under three scenarios: low flow year, normal flow year, and high flow year. The investigation delves into N and P concentrations, computes atmospheric deposition fluxes, and evaluates the loads and retention ratios of N and P in the urban river network of Tianjin City. Scenario analysis reveals distinct trends, with high flow years associated with elevated N and hydrodynamics levels, while low flow years exhibit contrasting patterns. Key findings include 1) wet deposition fluxes contributing 21% of NH4+-N and 9% of TP river loads, 2) continuous rainfall and runoff resulting in immediate or delayed increases in NH4+-N, TN, and TP concentrations in surface water, 3) an increase in average N:P mole ratios downstream along the Haihe River, and 4) N and P mean retention ratios were 48% and 63%, respectively. The study suggests that implementing sponge city construction and sustainable drainage systems may improve water quality by mitigating the direct discharge of rainfall runoff into the river.

Water addition but not reduction alters plant biomass-diversity relationship.

The relationship between plant aboveground biomass and diversity typically follows a unimodal pattern, showing a positive correlation in resource-poor habitats and a negative correlation in resource-rich environments. Precipitation is a crucial resource for both plant biomass and diversity in terrestrial ecosystems. However, the impact of precipitation changes on the relationship between plant biomass and diversity remains unclear. We conduct a water addition field experiment in a semiarid grassland and identify a unimodal relationship between plant biomass and species richness under ambient conditions. Water addition delays the declining phase of this unimodal curve and shift it upward compared to ambient conditions. Our meta-analysis of water addition experiments conducted across major biomes worldwide (grassland, shrubland, desert, and forest) supports this finding, while water reduction does not alter the biomass-diversity relationship. Water addition increases biomass in all climate but only increases species richness in arid and semiarid climate. Similarly, water reduction decreases biomass in all climate but only reduces species richness in arid and semiarid climate. Species richness in dry subhumid and humid climate does not change significantly. Furthermore, our field experiment shows that water addition increases plant diversity while decreasing soil inorganic nitrogen levels. The increase in one resource, such as water, leads to the scarcity of another, such as nutrient, thus postponing the declining phase of the plant biomass-diversity relationship typically observed in resource-rich habitats. Our research contributes to predicting the plant biomass-diversity relationship under changing precipitation conditions and highlights the complex interplay between water availability, nutrient level, and plant diversity.

Effects of biochanin A on lactational performance, nitrogen metabolism, and blood metabolites in dairy cows

Optimizing nitrogen utilization efficiency and mitigating nitrogen losses in cows plays a pivotal role in fostering economic sustainability within contemporary agricultural systems. Biochanin A (BCA), a natural component in red clover, has the potential to improve nitrogen metabolism in dairy cows. The primary objective of this study was to probe the impact of biochanin A supplementation on lactational performance, nitrogen metabolism, and blood metabolites in dairy cows. A complete randomized block design experiment was conducted over 28 d, involving 36 multiparous Holstein cows (comparable milk yield = 37.1 ± 2.90 kg, BW = 642 ± 70.0 kg, days in milk = 92 ± 8.0 d, and parity = 2.4 ± 0.50), which were allocated to three treatment groups: the control group (with 0 g/d BCA), the low group (with 10 g/d BCA), and the high group (with 40 g/d BCA). Biochanin A supplementation improved the lactational performance of cows by increasing milk yield by 6.3% (P = 0.007) and feed efficiency by 12.7% (P = 0.009). Total intestinal apparent digestibility was unaffected by BCA supplementation (P > 0.05), but microbial nitrogen was increased by 30.0% (P = 0.002) for promoting nitrogen utilization efficiency by 20.7% (P = 0.004). Milk competent yields (protein, lactose, and non-fat milk solid) were increased with increasing BCA supplementation (P < 0.05). Urea nitrogen levels in plasma and milk were both decreased by BCA supplementation (P < 0.05). Blood routine parameters and plasma biochemical parameters both received no effect by BCA supplementation (P > 0.05). BCA did not affect body health of dairy cows. Additionally, none of the plasma endocrine hormones were affected (P > 0.05). A total of 95 significantly different metabolites were screened from the plasma metabolites of cows in the BCA-added and non-added groups. After performing an enrichment analysis of the metabolic pathways associated with the different metabolites, six specific pathways were identified: bile acid biosynthesis, aspartate metabolism, pyrimidine metabolism, arginine and proline metabolism, the urea cycle, and ammonia recycling. The inclusion of BCA is suggested to enhance milk yield and modulate nitrogen metabolism by influencing relevant metabolites within the metabolic pathways.

Sunflower-nitrogen calibration based on the amount of mineral nitrogen in soil under rainfed conditions in thrace region

Sunflower is grown under both rainfed and irrigated conditions in all regions to meet the vegetable oil needs of Türkiye, however, in the Thrace region, it is the main rotation crop grown under rainfed conditions after wheat. This study was conducted in the Thrace Region between 2013 and 2016 in order to evaluate the parameters that can be used as an index for nitrogen fertilization recommendations for sunflower plant. For this purpose, organic matter and saturation were determined in soil samples taken from 0-20 cm depth before the experiment and ammonium and nitrate contents were determined in soil samples taken from 0-30 cm, 30-60 cm, and 60-90 cm depths. Within the scope of the study, 27 field trials were conducted over four years. Nitrogen levels of 0 (control), 3, 6, 9, and 12 kg da-1 were implemented in the trials conducted in a Latin square experimental design. In this study, log (279.9-y) = log279.9-0.090b1-0.117x Mitscherlich calibration equations were determined based on 0-30 cm ammonium+nitrate content in the soil.

Roles of M1 and M2 macrophage infiltration in post-renal transplant antibody-mediated rejection

BackgroundWe aimed to analyze the roles of M1 and M2 macrophage infiltration in post-renal transplant antibody-mediated rejection (AMR). MethodsA total of 102 recipients who underwent renal allotransplant from January 2020 to February 2023 were divided into an immune tolerance group (n = 56) and a rejection group (n = 46). The transplant renal biopsy specimens were harvested by ultrasound-guided puncture. The M1 and M2 macrophages in renal tissues were counted, and the M1/M2 ratio was calculated. The numbers of M1 and M2 macrophages and M1/M2 ratios in patients with different severities of interstitial fibrosis/tubular atrophy (IF/TA) and different degrees of tubulointerstitial inflammatory cell infiltration were compared. The predictive values of M1 and M2 macrophages and M1/M2 ratio for post-renal transplant AMR were clarified. ResultsThe rejection group had significantly more M1 and M2 macrophages and higher M1/M2 ratio than those of the immune tolerance group (P < 0.05). In the rejection group, infiltrating macrophages were mainly distributed in the glomerular and interstitial capillaries, with M1 macrophages being the predominant type. With increasing severity of IF/TA, the numbers of M1 and M2 macrophages and M1/M2 ratio rose in patients with post-renal transplant AMR (P < 0.05). The numbers and ratio had significant positive correlations with the levels of blood urea nitrogen and serum creatinine (P < 0.05). The areas under the curves (AUCs) of numbers and M1 and M2 macrophages and M1/M2 ratio for predicting post-renal transplant AMR were 0.856, 0.839 and 0.887, respectively. The combined detection had AUC of 0.911 (95% CI: 0.802–0.986), sensitivity of 90.43% and specificity of 83.42%. ConclusionsSignificant macrophage infiltration is present in the case of post-renal transplant AMR, and closely related to the severity of IF/TA and the degree of tubulointerstitial inflammatory cell infiltration.

The Impact of Artificial Afforestation on the Soil Microbial Community and Function in Desertified Areas of NW China

Afforestation is a widely used method of controlling desertification globally as it significantly impacts the soil quality, microbial community structure, and function. Investigating the effects of various artificial vegetation restoration models on soil microbial communities is crucial in understanding the mechanisms involved in combating desertification. However, research on this topic in arid, desertified regions is limited. In this study, we collected soil samples from two types of artificial forests (single species and mixed species) and bare desert soils in desertified areas of Northwest China to explore the impact of afforestation on soil nutrients, the microbial community composition, network relationships, and carbohydrate degradation abilities using metagenomic sequencing techniques. Our findings indicate that afforestation significantly enhances the soil moisture, total carbon, available phosphorus, and total nitrogen levels. The soil under mixed-species forests exhibited significantly higher levels of total carbon, total phosphorus, available phosphorus, and total nitrogen than that under single-species forests. Following afforestation, the populations of Pseudomonadota, Acidobacteriota, and Cyanobacteria increased significantly, whereas Actinomycetota decreased markedly. In single-species forests, Pseudomonadota and Bacillota were enriched, whereas Chloroflexota, Planctomycetota, and Acidobacteriota were more prevalent in mixed-species plantations. Afforestation increases the complexity and stability of microbial community networks. Afforestation enhances microbial metabolic activity, particularly increasing the abundance of carbon degradation functional genes in forest soils compared to bare desert soils. Mixed-species plantations outperform single-species forests in enhancing carbohydrate metabolism, amino acid metabolism, and the biodegradation and metabolism of xenobiotics. The abundance of functional genes associated with the degradation of starch, cellulose, hemicellulose, chitin, and pectin in mixed-species forests was significantly greater than in single-species plantations. Our study shows that mixed-species afforestation effectively improves the soil quality, enhances the stability of soil microbial communities, and bolsters the carbon cycle in arid regions prone to desertification. The reciprocal relationship between microorganisms and plants may serve as an intrinsic mechanism by which mixed-species afforestation more effectively controls desertification.

Further evaluation of a new method to investigate antimicrobial, colour retainment and slice healing properties of cucumber (Cucumis sativus L.) shows differences between conventional and organic production systems in inter-laboratory comparison trials

ABSTRACT Food quality is influenced by different cultivation methods and the type and amount of fertiliser used. The resulting vitality of the food and the ability to resist degradation processes were investigated using a vitality test method, assessing antimicrobial (AMP), colour retainment (CRP) and slice healing properties (SHP) of cucumber fruit (Cucumis sativus L.). Five hundred and sixty-four cucumber samples, grown in greenhouse conditions in Denmark and fertilised with different nitrogen (N) levels, were examined, testing cucumbers from conventional (Conv: 145 g N m−2), organic (OrgN-: 70 g N m−2) and biodynamic (BdN+: 122 g N m−2) cultivation regimes. The experimental design resulted in 54 comparisons consisting of six trial repetitions with three measured parameters examined in three laboratories. Every sample was sliced by a slicing tool, producing alternating slice thickness of 15 and 20 mm. The samples were reassembled, wrapped in transparent plastic foil and stored vertically in a heating cabinet for 14 days in the dark. Subsequently, AMP and CRP were scored visually based on a photo reference series. SHP were examined on the horizontally fixed cucumber sample by attaching weights to two specific slices one after the other until the slices detached. The evaluation of the cucumbers analysed by the vitality test method in inter-laboratory comparison trials led to consistent results. Cucumbers of the OrgN- and BdN+ variants scored higher in the test than Conv. It was not possible to differentiate OrgN- vs. BdN+ cucumbers. The vitality test is an attractive method for evaluating post-harvest resilience in cucumbers.

Response of N, P, and metal ions in deep soil layers to long-term cultivation of rubber and rubber-based agroforestry systems

The growing demand for natural rubber products has driven the expansion of rubber plantations in recent decades. While much attention has been given to studying the long-term effects of rubber and rubber-based agroforestry systems on surface soil properties, there has been a tendency to overlook changes in soil properties in deeper layers. Our study addresses this gap by examining alterations in nitrogen (N), phosphorus (P), and metal ion levels in deep soil layers resulting from the prolonged cultivation of rubber and rubber-based agroforestry systems. We found notable shifts in soil NH4+ and NO3− concentrations within the 0–30 cm soil layer across different-aged rubber and rubber-based agroforestry systems. Particularly in mature systems, NO3− and available P levels were close to zero below 30 cm soil depth. Introducing Flemingia macrophylla into young rubber plantations increased soil NH4+ and NO3− in the 0–90 cm soil layer and available P in the 0–10 cm soil layer. Over the long term, cultivation of rubber plantations increased the depletion of total P in the 0–50 cm soil layer, available iron (Fe) and manganese (Mn) in the 30–90 cm soil layer, available copper (Cu) and zinc (Zn) in the 0–90 cm soil layer, accompanied by a decrease in soil pH and increase in exchangeable aluminum (Al) in the 0–90 cm soil layer. Notably, soil exchangeable Al levels exceeding 2.0 cmol kg−1 appeared to induce aluminum toxicity. Furthermore, soil pH below 5.2 triggered a sharp release of exchangeable Al within the 0–90 cm soil layer of rubber plantations, with soil available P nearing zero when exchangeable Al levels assed 7.3 cmol kg−1. Our findings underscore the profound impact of long-term rubber plantation cultivation on surface and deep soil properties. Addressing soil degradation in these deep soil layers poses significant challenges for future soil restoration efforts.

Effects of Actinomycete Act12 on Soil Microbial Community Structure and Plant Traits of Ziziphus jujuba Mill.

Biocontrol agents play a crucial role in agricultural production by reducing the use of pesticides and chemical fertilizers, controlling pests and diseases, and enhancing crop yield and quality. While extensive research has focused on the growth-promoting and yield-increasing effects of biocontrol agents on herbaceous plants, their impact on woody crops such as trees and shrubs has not been reported. This study investigates the effects of the actinomycete strain Act12, applied via root drenching and foliar spraying, on the flowering and fruit set rates, leaf physiology and enzyme activity, and fruit characteristics and yield of the jujube tree (Ziziphus jujuba Mill.). Additionally, this study analyzes the physicochemical properties of the soil and the diversity and community structure of its microorganisms. The results indicate that treatment with Act12 significantly altered the α and β diversity of soil microorganisms and enhanced the interaction networks among them. This led to increased levels of available nitrogen, phosphorus, and potassium in the soil, significantly improving nutrient availability. Consequently, there was an improvement in the number of flower buds and fruits, as well as an increase in the longitudinal diameter and individual fruit weight of the jujube. These effects significantly enhanced the yield and total sugar content of the winter jujube. Given these findings, Act12 can be considered an effective microbial agent for enhancing the yield and quality of winter jujube when applied to the soil.

Growth and Yield Responses of Bari Sweet Corn-1 (Zea Mays L.) to Naa at Different Nitrogen Levels

A field experiment was conducted to investigate the effect of foliar application of NAA at varying N-levels on growth and yield responses of BARI Sweet Corn-1 (Zea mays L.). The trial consisted of ten treatments viz. control = without NAA and N fertilizer; RDN (recommended dose of N fertilizer); 25 ppm NAA + RDN; 50 ppm NAA + RDN; 25 ppm NAA + 50% higher RDN; 50 ppm NAA + 50% higher RDN; 25 ppm NAA + 100% higher RDN; 50 ppm NAA + 100% higher RDN; 25 ppm NAA+ 150% higher RDN and 50 ppm NAA + 150% higher RDN. Results showed that application NAA in combination with various nitrogen (N) levels produced better growth than control but with few exceptions. Growth parameters viz. total dry mater (TDM), leaf area (LA), relative growth rate (RGR) and net assimilation rate (NAR) were recorded maximum from 25 ppm NAA in combination with RDN treatment. Findings revealed that 25 ppm NAA in combination with RDN treatment had also produced higher number of leaves per plant, specific leaf area (SLA), biomass duration (BMD) and absolute growth rate (AGR) than RDN treatment although statistically at par to each other. The maximum number of leaves per plant, shoot-root ratio and SLA although recorded maximum from 25 ppm NAA in combination with 100% higher RDN treatment but statistically similar to rest of the combined treatments. Yield attributes and yield of BARI Sweet Corn-1 showed positive response to NAA at varying N-levels with significant variations in majority of cases. The maximum number of cobs and productive cobs per plant, dry weight of cobs, length of cob, number of seed per row and cob, dry weight of tassel, yield per plant and harvest index were also obtained from 25 ppm NAA in combination with RDN treatment. However, maximum number of cobs and productive cobs per plant were also noted from 25 ppm NAA in combination with 100% higher RDN treatment. Significantly maximum yield per plant (114.41g) obtained from 25 ppm NAA in combination with RDN treatment which was 12.02% higher over RDN treatment. Out of ten treatments, foliar application of 25 ppm NAA in combination with RDN treatment produced better stimulation in growth and yield of BARI Sweet Corn-1. Bangladesh J. Bot. 53(2): 337-344, 2024 (June)

Whey protein hydrolysate maintains the homeostasis of muscle metabolism in exercise mice and releases potential anti-fatigue peptides after gastrointestinal digestion

Whey protein hydrolysate (WPH) has been proven to possess anti-fatigue effects, but its regulatory mechanism on muscle metabolism in exercise mice is unclear. In the present study, the effects of WPH on muscle metabolites in mice before and after exercise were explored using metabolomics techniques. Results showed that 34 muscle metabolites were significantly altered by exercise in the WPH group, which was less than 71 in the WP group and 101 in the control group. Specifically, supplementation of WPH significantly increased the levels of amino acid and carbohydrate metabolites (i.e., 1-methylhistamine, guanidoacetic acid, melibiose, and xylulose 5-phosphate) pre-exercise, thereby delaying energy depletion and maintaining metabolic homeostasis during exercise. Furthermore, simulated gastrointestinal digestion (SGID), peptidomics and bioinformatics tools were employed to screen potential peptides in WPH. A total of 37 peptides with 2–5 residues in WPH were resistant to SGID (peak intensity > 105). Among them, YGLF showed favorable potentials in physicochemical property, bioavailability, bioactivity, SwissTargetPrediction, and molecular docking. In addition, the anti-fatigue activity of YGLF was evaluated in vivo. YGLF (100 mg/kg) significantly increased the contents of glucose, glycogens, and amino acids and decreased the levels of lactate and urea nitrogen in mice after exercise. These findings may serve as a reference for the screening of anti-fatigue peptides.

Effect of Different Doses of Nitrogen on Growth and Grain Yield of Hybrid Maize (Zea mays L., Gold 97)

This study investigates the impact of various nitrogen doses on the growth and grain yield of hybrid maize (Zea mays L., Gold 97). Nitrogen plays an important role in crop phenology, morphology, and grain yield of maize plants. A field experiment was conducted in a randomized complete block design with six levels of nitrogen (150, 160, 170, 180, 190, and 200 kg/ha) in four replications to evaluate the effect of different doses of nitrogen on the growth and yield of maize. Germination percentage, Days to 75% tasselling and silking, plant height, number of leaves, leaf area (LAI), cobs length, grain per row, pod, and total grain yield were recorded. Maximum germination was found in 180 kg N /ha. Plant height, number of leaves, and leaf area (LAI) were found to be maximum in the plots treated with 180-200 kg/ha of N. This field experiment revealed that different nitrogen dose has significant effects on days to 75% Tasselling and silking and found to be maximum in 180 kg/ha N treated plot. Also, nitrogen has a significant effect on cobs length, and grain per row was found highest at 180 kg N/ha. The highest Grain yield was found 180 kg/ha N treated plot. Findings have demonstrated that increasing nitrogen levels can often lead to increased grain yield up to a certain point, after which the yield plateaus or even declines. These findings provide valuable guidance for optimizing agricultural practices to meet the increasing global demand for cereal crops.

Geospatial Analysis of Soil Fertility in Muzaffarpur District, Bihar, India: Integrating GPS and GIS Technologies

The soil fertility maps generated using Global Positioning System (GPS) and Geographic Information Systems (GIS) serve as crucial tools for effective nutrient management decision-making. However, soil fertility data for the Minapur, Kanti, and Marwan blocks of Muzaffarpur district, Bihar, India was found to be insufficient. Therefore, a soil fertility inventory research was conducted in these three blocks to create thematic soil fertility maps. A total of 40 geo-referenced composite soil samples were collected from various locations within the study area using a handheld GPS device. The processed soil samples were analyzed for various soil fertility parameters using standard methods. Soil nutrient status and fertility maps were then created using ArcGIS software with Inverse Distance Weighted (IDW) interpolation techniques. The results clearly indicated that the soil reaction was alkaline, with a pH value exceeding 7.5. The content of soil organic matter, potassium, and sulphur was found to be low to medium, while available nitrogen and phosphorus levels were recorded as very low in these blocks. Finally it is concluded that the study generated thematic soil fertility maps for Minapur, Kanti, and Marwan blocks of Muzaffarpur district, Bihar, revealing alkaline soil with low to medium organic matter, potassium, and sulphur and very low nitrogen and phosphorus levels.

Exogenous regulation of macronutrients promotes the accumulation of alkaloid yield in anisodus tanguticus (Maxim.) pascher

BackgroundAnisodus tanguticus (Maxim.) Pascher (A. tanguticus) is a valuable botanical for extracting tropane alkaloids, which are widely used in the pharmaceutical industry. Implementing appropriate cultivation methods can improve both the quality and yield of A. tanguticus. A two-year field experiment was conducted from 2021 to 2023 using a single-factor randomized complete block design replicated three times. The study examined the effects of different nutrient levels (nitrogen: 0, 75, 150, 225, 300, 375 kg/ha; phosphorus: 0, 600, 750, 900, 1050, 1200 kg/ha; potassium: 0, 75, 112.5, 150, 187.5, 225 kg/ha) on the growth, primary alkaloid contents, and alkaloid yield of A. tanguticus at different growth stages (S-Greening, S-Growing, S-Wilting; T-Greening, T-Growing, and T-Wilting) in both the roots and aboveground portions.ResultsOur results demonstrate that nutrient levels significantly affect the growth and alkaloid accumulation in A. tanguticus. High nitrogen levels (375 kg/ha) notably increased both root and aboveground biomass, while phosphorus had a minimal effect, especially on aboveground biomass. For alkaloid content (scopolamine, anisodamine, anisodine, atropine), a moderate nitrogen level (225 kg/ha) was most effective, followed by low potassium (75 kg/ha), with phosphorus showing a limited impact. Increased phosphorus levels led to a decrease in scopolamine content. During the T-Growing period, moderate nitrogen addition (225 kg/ha) yielded the highest alkaloid levels per unit area (205.79 kg/ha). In the T-Wilting period, low potassium (75 kg/ha) and low phosphorus (750 kg/ha) resulted in alkaloid levels of 146.91 kg/ha and 142.18 kg/ha, respectively. This indicates nitrogen has the most substantial effect on alkaloid accumulation, followed by potassium and phosphorus. The Douglas production function analysis suggests focusing on root biomass and the accumulation of scopolamine and atropine in roots to maximize alkaloid yield in A. tanguticus cultivation.ConclusionsOur findings show that the optimum harvesting period for A. tanguticus is the T-Wilting period, and that the optimal nitrogen addition is 225 kg/ha, the optimal potassium addition is 75 kg/ha, and the optimal phosphorus addition is 600 kg/ha or less.

Betaine ameliorates arsenic-induced kidney injury in mice by mitigating oxidative stress-mediated inflammation

Arsenic, an environmental pollutant and poisonous metalloid, has adverse effects on different body organs, including the kidneys. Betaine is a natural nutrient that has many beneficial health effects. This research was conducted to examine the impact of betaine on nephrotoxicity caused by inorganic arsenic (NaAsO2) in mice. Mice were separated into following groups: control, NaAsO2 (50 ppm), NaAsO2 (50 ppm) + betaine (500 mg/kg), and betaine (500 mg/kg). Mice were received NaAsO2 via drinking water for 8 consecutive weeks and betaine was given to the animals via gavage once daily in the 7th and 8th weeks of the study. Upon completion of the study, the mice were euthanized and samples of serum and kidney were obtained for further evaluations. Administration of NaAsO2 increased the levels of blood urea nitrogen and creatinine in the serum. It enhanced the amounts of renal malondialdehyde and decreased the total thiol levels, as well as the activity of antioxidant enzymes (catalase, superoxide dismutase, and glutathione peroxidase). Furthermore, it enhanced the levels of renal inflammatory indicators (tumor necrosis factor-alpha and nitric oxide). Western blot results exhibited an increase in the protein expression of nuclear factor kappa B (NF-κB), and phosphorylated NF-κB in NaAsO2-treated mice. Histopathological results also confirmed kidney damage caused by NaAsO2. However, treatment with betaine improved NaAsO2-related kidney injuries in mice. The results of this work indicated that betaine can attenuate kidney damage caused by NaAsO2 by inhibiting oxidative stress and inflammation.

Metabolomic characterization of Chinese sturgeon (Acipenser sinensis) in semi-intensive and recirculating aquaculture systems

The present study investigated the blood biochemistry and metabolic profiles of Chinese sturgeons (Acipenser sinensis) reared in semi-intensive traditional aquaculture systems (TAS) and recirculating aquaculture systems (RAS). Results of serum biochemical indices in the RAS group showed that the levels of total protein (TP), albumin (ALB), globulin (GLO), high-density lipoprotein cholesterol (HDL), low-density lipoprotein cholesterol (LDL), cholesterol (CHOL), and alkaline phosphatase (ALP) were higher than those in the TAS group (p < 0.05). TAS group induced significantly higher levels of glucose (GLU), triglycerides (TG), aspartate transaminase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH), lactic acid (LD), and blood urea nitrogen (BUN) than RAS group (p < 0.05). Additionally, a total of 3123 metabolites were detected, including 152 up-regulated and 861 down-regulated metabolites, totally 1013 metabolites were significantly different (VIP > 1 and p < 0.05). The analysis of these different metabolites revealed that the top four significantly metabolic pathways (p-value or FDR < 0.05) were neomycin, kanamycin, and gentamicin synthesis, glycerophospholipid metabolism, folate biosynthesis, and phosphatidylinositol signaling system, which are related to aminoglycosides, lipids, and pterin metabolism. There were 28 metabolites enriched in the four metabolic pathways, including 7 different types of antibiotics, 16 glycerophospholipids, and 5 pterins. Overall, Chinese sturgeons reared in TAS showed higher concentrations of 25 metabolites, and only three metabolites with higher significant activities showed in RAS, including PE (18:1(9Z)/22:6 (4Z, 7Z, 10Z, 13Z, 16Z, 19Z)), PC (14:0/P-18:1(9Z)), and Folic Acid. In conclusion, our results revealed differences in the biochemical indices, metabolites, and metabolic pathways of Chinese sturgeons reared in TAS and RAS and confirmed that RAS improves fish metabolism and physiological state, providing new insights for sturgeon aquaculture.

Effect of nitrogen fertilizer on the quality traits of Indica rice with different amylose contents.

Nitrogen is a key factor affecting the quality of rice. Studying the impact of nitrogen fertilizer on the taste, physicochemical properties, and starch structure of Indica rice with different amylose contents is of great significance for scientifically fertilizing and cultivating high-quality rice varieties for consumption. The results indicate that increasing nitrogen fertilizer application reduces the amylose content and increases the protein content, resulting in a decrease in taste quality. Simultaneously, it reduces the intergranular porosity of starch particles, improving the appearance and milling quality of rice. Compared to the N1 treatment (nitrogen fertilizer application rate of 90 kg ha-1), the taste of low-amylose rice (Yixiangyou 2115) and high-amylose rice (Byou 268) decreased by 14.24% and 19.79%, respectively, under N4 treatment (nitrogen fertilizer application rate of 270 kg ha-1). The effect of nitrogen fertilizer on low-amylose rice is mainly reflected in increased rice hardness, enthalpy value, and setback viscosity, resulting in a decline in taste. The effect of nitrogen fertilizer on high-amylose rice is mainly reflected in a decrease in peak viscosity, an increase in gelatinization temperature, and crystallinity under high nitrogen levels. Increasing nitrogen fertilizer application can improve the appearance and milling quality of rice, but it also leads to an increase in protein content, hardness, gelatinization enthalpy, decrease in breakdown value, and a decline in palatability. In practical production, different production measures should be taken according to different production goals. © 2024 Society of Chemical Industry.