Rabbit kidney function was assessed in vitro after cryoprotection with either 3 or 4 M dimethyl sulfoxide. The introduction and removal of the cryoprotectant was carried out in a stepwise progressive manner and the removal in a stepwise progressive manner with hypertonic mannitol solutions. This in vitro model can be shown to respond to various ischemic-like states resulting in poor or absent function. Active tubular transport can be demonstrated. It has been used by many authors as an intermediate step prior to the ultimate test of reimplant and contralateral nephrectomy. Variations in the rate of cooling at cryoprotection levels of 3 and 4 M dimethyl sulfoxide concentration (Me 2SO) were carried out. In general, at 3 M concentration of Me 2SO, creatinine clearance, sodium and glucose reabsorption are preserved with a fair degree of success after cooling to −10, −15, and −20 °Cin our model, when the rate of cooling to these levels is 1.0 °C/min. When a cooling rate of 0.5 °C/min is used, renal function is significantly reduced whether the final temperature is −10, −15, or −20 °C. Control rabbit kidneys will tolerate 4 M concentration of Me 2SO and give fairly good function. When cooled to −15 or −20 °C, there is poor function at 0.1 and at 0.5 °C/min. Fair function is obtained at the rate of 1 °C/min to −10 °C. Therefore, at cryoprotectant levels of 3 and 4 M Me 2SO, kidney function as assayed by in vitro perfusion, is better when the cooling rate is 1.0 °C/min.