Levels Of Bioactive Molecules Research Articles

The nature of changes in endocrine and immune factors at the initial stage of the formation of chronic wounds

Aim. The work aimed to study the nature of changes in endocrine signals (insulin and cortisol) and bioactive molecules that control inflammation and activation of process synthesis (interleukin-2 (IL-2), interleukin-8 (IL-8), vascular endothelial growth factor (VEGF), amino-terminal propeptide of type III procollagen (PIIINP)), at the initial stage of the formation of chronic wounds in the experiment.
 Materials and methods. The study was conducted in 2 groups of Wistar rats. The first group is represented by intact animals. Animals of the second group were modeled chronic wounds. On the 3rd day of the experiment, all animals were euthanized. The concentration of studied bioactive molecules was measured in blood serum by enzyme immunoassay. Selected areas of the wound were subjected to histological examination.
 Results. The levels of cortisol, insulin, VEGF, IL-2, and PIIINP in animals with chronic wounds at the stage of inflammation were significantly higher compared to intact animals. A tendency towards a decrease in the expression of IL-8 was shown. Relationships between the levels of bioactive molecules in animals of different groups were studied. According to the results of studies a correlation between the concentrations of IL-8 and cortisol in rats of the intact group was established. In the group of rats on the 3rd day after wound induction, a positive correlation was noted between the levels of IL-8 and IL-2. Wound defects healed under the scab. Microscopic analysis showed that the histoarchitectonics of tissues in the area of damage on the 3rd day corresponded to the classic manifestations of the process of healing wounds at this stage. The main part of the wound was filled with fibrin strands with a predominantly inflammatory cellular component. In some areas, signs of growth and development of young granulation tissue were noted. In the marginal sections, signs of re-epithelialization of the wound defect were observed.
 Conclusions. Even though in our study, the histoarchitectonics of tissues in the area of damage corresponded to the classical manifestations of the wound healing process at the stage of inflammation, the obtained changes and the relationship between endocrine and immune factors may be harbingers of the formation of the chronic wound in the future.

Platelet Lysate and Non-Healing Ulcers

Platelet lysate contained a wide range of bioactive molecules involved in cell proliferation, migration. The paper analyzes the possibility of using platelet lysate for the treatment of non-healing ulcers. The levels of bioactive molecules were determined by ELISA in platelet lysate. Efficacy of treatment with platelet lysate patients with diabetic foot ulcers, ulcers in patients with peripheral arterial disease and venous insufficiency were analyzed on wound surface area, total epithelialization. Twice injection of platelet lysate with an interval of 5-7 days possess to wound surface area closure on Day 180 at 96% in patients with diabetic foot ulcers, while in patients with peripheral arterial disease and venous insufficiency ulcers wound closure was less. Platelet lysate from patients with non-healing ulcers contained growth factor, cytokine, components of the extracellular matrix, and nitric oxide. Between levels of bioactive molecules in platelet lysate and outcome were found significant correlation. The obtained results indicate that platelet lysate possesses wound healing activity.

Changes in phytohormone content and associated gene expression throughout the stages of pear (Pyrus pyrifolia Nakai) dormancy.

To elucidate the role of phytohormones during bud dormancy progression in the Japanese pear (Pyrus pyrifolia Nakai), we investigated changes in phytohormone levels of indole acetic acid (IAA), gibberellic acid (GA), abscisic acid (ABA) and trans-zeatin (tZ). Using ultra-performance liquid chromatography/mass spectrometry/mass spectrometry, we monitored phytohormone levels in the buds of field-grown and potted trees that were artificially heated to modify the timing of dormancy and flowering (spring flush) progression. We also analyzed the expression of GA- and ABA-metabolic genes during dormancy. Indole acetic acid and tZ levels were low during dormancy and increased toward the flowering stage. Gibberellic acid levels were maintained at relatively high concentrations during the dormancy induction stage, then decreased before slightly increasing prior to flowering. The low GA concentration in potted trees compared with field-grown trees indicated that GA functions in regulating tree vigor. Abscisic acid levels increased from the dormancy induction stage, peaked near endodormancy release and steadily decreased before increasing again before the flowering stage. The ABA peak levels did not always coincide with endodormancy release, but peak height correlated with flowering uniformity, suggesting that a decline in ABA concentration was not necessary for resumption of growth but the abundance of ABA might be associated with dormancy depth. From monitoring the expression of genes related to GA and ABA metabolism, we inferred that phytohormone metabolism changed significantly during dormancy, even though the levels of bioactive molecules were consistently low. Phytohormones regulate dormancy progression not only upon the reception of internal signals but also upon sensing ambient conditions.