Platelet Lysate and Non-Healing Ulcers

Abstract

Platelet lysate contained a wide range of bioactive molecules involved in cell proliferation, migration. The paper analyzes the possibility of using platelet lysate for the treatment of non-healing ulcers. The levels of bioactive molecules were determined by ELISA in platelet lysate. Efficacy of treatment with platelet lysate patients with diabetic foot ulcers, ulcers in patients with peripheral arterial disease and venous insufficiency were analyzed on wound surface area, total epithelialization. Twice injection of platelet lysate with an interval of 5-7 days possess to wound surface area closure on Day 180 at 96% in patients with diabetic foot ulcers, while in patients with peripheral arterial disease and venous insufficiency ulcers wound closure was less. Platelet lysate from patients with non-healing ulcers contained growth factor, cytokine, components of the extracellular matrix, and nitric oxide. Between levels of bioactive molecules in platelet lysate and outcome were found significant correlation. The obtained results indicate that platelet lysate possesses wound healing activity.