Levels Of Xylanase Research Articles

Alteration of Cecal Microbiota by Antimicrobial Peptides Enhances the Rational and Efficient Utilization of Nutrients in Holstein Bulls.

We previously observed that supplementation with antimicrobial peptides facilitated the average daily weight gain, net meat, and carcass weights of Holstein bulls. To expand our knowledge of the possible impact of antimicrobial peptides on cecum microbiota, further investigations were conducted. In this study, 18 castrated Holstein bulls with insignificant weight differences and 10 months of age were split randomly into two groups. The control group (CK) was fed a basic diet, whereas the antimicrobial peptide group (AP) was supplemented with 8 g of antimicrobial peptides for 270 days. After slaughter, metagenomic and metabolomic sequencing analyses were performed on the cecum contents. The results showed significantly higher levels of amylase, cellulase, protease, and lipase in the CK than in the AP group (P ≤ 0.05). The levels of β-glucosidase and xylanase (P ≤ 0.05), and acetic and propionic acids (P ≤ 0.01), were considerably elevated in the AP than in the CK group. The metagenome showed variations between the two groups only at the bacterial level, and 3258 bacteria with differences were annotated. A total of 138 differential abundant genes (P < 0.05) were identified in the CAZyme map, with 65 genes more abundant in the cecum of the AP group and 48 genes more abundant in the cecum of the CK group. Metabolomic analysis identified 68 differentially expressed metabolites. Conjoint analysis of microorganisms and metabolites revealed that Lactobacillus had the greatest impact on metabolites in the AP group and Brumimicrobium in the CK group. The advantageous strains of the AP group Firmicutes bacterium CAG:110 exhibited a strong symbiotic relationship with urodeoxycholic acid and hyodeoxycholic acid. This study identified the classification characteristics, functions, metabolites, and interactions of cecal microbiota with metabolites that contribute to host growth performance. Antimicrobial peptides affect the cecal microorganisms, making the use of nutrients more efficient. The utilization of hemicellulose in the cecum of ruminants may contribute more than cellulose to their production performance.

Effect of Extruder Configuration and Extrusion Cooking Processing Parameters on Selected Characteristics of Non-Starch Polysaccharide-Rich Wheat Flour as Hybrid Treatment with Xylanase Addition

The effects of a single-screw extruder configuration and processing variables such as conventional extrusion or hybrid treatments with xylanase were tested on the extrusion performance and selected characteristics of the developed non-starch polysaccharide-rich (NSP-rich) wheat flour. L/D 16 and 20 extruder configurations with various screw profiles were used. The interactions between processing variables (moisture content 23, 25, 27%; screw speed 40, 60, 80 rpm; xylanase level 0, 50, 100 ppm) were assessed to indicate energy consumption and the rheological properties of flour. The results showed that the possibility of obtaining enzyme-assisted extruded flour products derived from flours of varying characteristics depended on the processing conditions. The application of various extruder configurations and screw profiles showed significant effects on both processing behavior and rheological characteristics. The longer L/D 20 extruder configuration using a screw profile with mixing elements allowed us to obtain products with lower extrusion pressure (max. 20.8 bar) and energy requirements (max. SME = 33.1 kWh/kg) and better rheological properties (max. Hyd = 69.2%, less intensive starch gelatinization with max. C3 = 1.47 Nm) than the L/D 16 version. The extruded wheat flour was characterized by improved hydration properties and limited retrogradation tendency, especially when hybrid extrusion with xylanase was applied. This may lead to favorable results, as the newly developed enzymatic extrusion modification method produces NSP-rich wheat flour with specific techno-functional and rheological characteristics that can be seen as a potential “clean label” enhancer in bakery products. Our statistical analysis confirmed feed moisture and screw speed to be the variables with the most significant effect on wheat flour features.

45 Awardee Talk: Investigation of the nutritional and functional roles of a combinational use of xylanase and β-glucanase on intestinal health and growth of nursery pigs

Abstract The objective of this study was to evaluate the intestinal health and growth performance of nursery pigs affected by dietary supplementation of increasing levels of xylanase and b-glucanase combinations. Pigs [n = 40; initial body weight (BW) = 6.5 ± 0.4 kg] were assigned to 5 dietary treatments and fed for 35 d in 3 phases (11, 9, and 15 d, respectively). Basal diets mainly included corn, soybean meal, and distiller’s dried grains with solubles with phytase (750 FTU/kg) supplemented with 5 levels of xylanase and b-glucanase combinations (NSPase) at 1) 0, 2) 280 TXU/kg xylanase and 125 TGU/kg b-glucanase, 3) 560 and 250, 4) 840 and 375, or 5) 1,120 and 500, respectively. Growth performance and fecal score were measured for each phase. On d 35, all pigs were euthanized and collected jejunal mucosa, jejunal digesta, jejunal tissues, and ileal digesta to determine the effects of increasing NSPase levels (%) and NSPase intake on jejunal mucosa-associated microbiota, jejunal digesta viscosity, immune responses, intestinal morphology, feed digestibility, and growth performance. Increasing NSPase level quadratically decreased (P &amp;lt; 0.05) Bacteroidetes (min: 0.7% at 636 TXU/kg and 284 TGU/kg), Prevotellaceae (min: 0.6% at 630 TXU/kg and 281 TGU/kg) in the jejunal mucosa. Increasing NSPase intake quadratically increased (P &amp;lt; 0.05) Firmicutes (max: 72.7% at 604 TXU/kg and 270 TGU/kg), Lactobacillaceae (max: 40.3% at 608 TXU/kg and 271 TGU/kg), and Lactobacillus (max: 40.3% at 608 TXU/kg and 271 TGU/kg) in the jejunal mucosa. Increasing NSPase intake also quadratically decreased (P &amp;lt; 0.05) Proteobacteria (min: 3.8% at 562 TXU/kg and 251 TGU/kg), Helicobacteraceae (min: 1.6% at 560 TXU/kg and 250 TGU/kg), and Helicobacter (min: 1.6% at 560 TXU/kg and 250 TGU/kg) in the jejunal mucosa. Increasing NSPase intake tended to quadratically decrease (P = 0.059) viscosity of jejunal digesta reaching the minimum (1.74 mPa·s) at 751 TXU/kg and 335 TGU/kg. Increasing NSPase level tended to linearly decrease (P = 0.073) jejunal IgG and tended to quadratically increase (P = 0.085) jejunal villus height to crypt depth ratio reaching the maximum (2.62) at 560 TXU/kg and 250 TGU/kg. Increasing NSPase intake tended to linearly increase the apparent ileal digestibility of dry matter (P = 0.087) and ether extract (P = 0.065). Increasing NSPase intake linearly increased (P &amp;lt; 0.05) average daily gain (ADG). In conclusion, increasing the combinational use of xylanase and β-glucanase positively modulated jejunal mucosa-associated microbiota, reduced jejunal digesta viscosity, reduced intestinal humoral immune response, enhanced villus morphology, increased ileal digestibility of nutrients, and increased growth of nursery pigs. The beneficial effects were maximized when xylanase and β-glucanase were supplemented at a range of 550 to 800 TXU/kg and 246 to 357 TGU/kg, respectively.

PSI-14 Effect of dietary xylanase supplementation on growth performance, nutrient digestibility, and digesta viscosity of weaned pigs fed wheat-soybean meal-based diets

Abstract Xylanase supplementation can improve nutrient digestibility in pig diets by degrading cell wall non-starch polysaccharides and reducing digesta viscosity as well as promoting intestinal health of pigs after weaning. This study determined the effects of dietary xylanase supplementation on growth performance, apparent ileal digestibility (AID) and apparent total tract digestibility (ATTD) of nutrients, and digesta viscosity in different digestive sites of weaned pigs. A total of 312 weaned pigs [body weight (BW) = 5.1 ± 0.9 kg] were weaned into 48 pens and assigned to one of six dietary treatments, each treatment with 8 pens, 6 to 7 pigs per pen, and sex ratios maintained within BW blocks. Experimental diets were formulated in a 3-phase nursery feeding program: Phase 1 (d 0 - d 7), Phase 2 (d 8 - d 21), and Phase 3 (d 22 – d 42). Experimental diets consisted of a wheat/soybean meal-based diet formulated to meet pig requirements (positive control, PC), the PC diet with reduction of 100 kcal of ME (negative control, NC), and the NC diet supplemented with either 900, 1,800, 3,600, or 7,200 units of xylanase. Feed disappearance and body weight were measured at d 7, 14, 21, and 42. Pen fecal score was assessed daily during d 0 to 14 and three times a week during d 15 to 28. On d 21 to 24 of the experiment (12 pigs per day), one pig per pen was selected for sample collection: ileal, cecal, and mid-colon digesta for viscosity and ileal digesta and feces for nutrient digestibility. Orthogonal polynomial contrasts were used to determine the linear and quadratic effects of increasing levels of xylanase in NC diets. Contrast analysis was used to compare PC and NC groups with xylanase-supplemented groups. Fecal score data were analyzed using the PROC FREQ procedure. Supplementing xylanase to NC diets did not improve pig growth performance (P &amp;gt; 0.05) through the nursery period. In wk 2 and wk 3, pigs fed the PC and NC diets had a greater (χ2 &amp;lt; 0.05) incidence of fecal scores 3 &amp; 4 (diarrhea) than the xylanase-supplemented pigs. The AID of DM and ATTD of crude protein, gross energy, and NDF were greater (P &amp;lt; 0.05) in the PC and 900-xylanase supplemented diets than in the other dietary treatments. In addition, the ATTD of NDF and ADF increased linearly (P &amp;lt; 0.1) in response to xylanase supplementation. Xylanase supplementation (900 to 7,200 U) decreased digesta viscosity in colon when compared with PC and NC diets. Overall, xylanase supplementation can improve nutrient digestibility, particularly at total tract level, and reduce viscosity in the hindgut, which could be related with decreasing the occurrence of looseness. However, the effects on growth performance were not expressive when xylanase was supplemented to wheat-soybean meal-based diets with reduction of 100 kcal of ME.

Effects of dietary xylanase supplementation on growth performance, intestinal health, and immune response of nursery pigs fed diets with reduced metabolizable energy.

This study aimed to investigate the effects of xylanase on growth performance and intestinal health of nursery pigs fed diets with reduced metabolizable energy (ME). One hundred ninety-two pigs at 8.7 kg ± 0.7 body weight (BW) after 7 d of weaning were allotted in a randomized complete block design with initial BW and sex as blocks. Eight dietary treatments consisted of 5 ME levels (3,400, 3,375, 3,350, 3,325, and 3,300 kcal ME/kg) below the NRC (2012) requirement and 4 levels of xylanase (0, 1,200, 2,400, and 3,600 XU/kg) to a diet with 3,300 kcal ME/kg. All pigs received their respective treatments for 35 d in 2 phases, pre-starter (14 d) and starter (21 d). On day 35, eight pigs in 3,400 kcal/kg (CON), 3,300 kcal/kg (LE), and 3,300 kcal/kg + 3,600 XU xylanase/kg (LEX) were euthanized to collect jejunal tissues and digesta for the evaluation of mucosa-associated microbiota, intestinal immune response, oxidative stress status, intestinal morphology, crypt cell proliferation, and digesta viscosity as well as ileal digesta to measure apparent ileal digestibility. Data were analyzed using the MIXED procedure on SAS 9.4. The LE increased (P < 0.05) jejunal digesta viscosity, tended to have decreased (P = 0.053) relative abundance of Prevotella, and tended to increase (P = 0.055) Lactobacillus. The LE also increased (P < 0.05) the concentration of protein carbonyl whereas malondialdehyde, villus height (VH), villus height to crypt depth ratio (VH:CD), apparent ileal digestibility (AID) of nutrients, and finally average daily feed intake were decreased (P < 0.05). The LE did not affect average daily gain (ADG). The LEX decreased (P < 0.05) digesta viscosity, increased (P < 0.05) the relative abundance of Prevotella, decreased (P < 0.05) Helicobacter, decreased (P < 0.05) the concentration of protein carbonyl, tended to increase (P = 0.065) VH, and decreased (P < 0.05) VH:CD and crypt cell proliferation. Moreover, LEX increased (P < 0.05) the AID of dry matter and gross energy and tended to increase (P = 0.099; P = 0.076) AID of crude protein, and ether extract. The LEX did not affect ADG but did tend to decrease (P = 0.070) fecal score during the starter phase. Overall, reducing ME negatively affected intestinal health parameters and nutrient digestibility without affecting growth. Supplementation of xylanase mitigated some of the negative effects observed by ME reduction on intestinal health and digestibility of nutrients without affecting growth.

Xylanase and beta-glucanase improve performance parameters and footpad dermatitis and modulate intestinal microbiota in broilers under an Eimeria challenge

Coccidiosis is an enteric disease of poultry worldwide that compromises gut health and growth performance. The current research investigated the effects of 2 doses of a multienzyme preparation on broilers' performance, gut health, and footpad dermatitis (FPD) under an Eimeria challenge. A total of 512 mixed-sex day-old chicks (Cobb 500) were randomly allocated to 4 treatments of 8 replicates. Treatments were: 1) nonchallenged control (NC); 2) NC+Eimeria challenge (CC); 3) CC+recommended level of xylanase and glucanase (XG, 100 g/t feed [on top]); 4) CC+double XG (2XG, 200 g/t feed). Eimeria spp. vaccine strains were gavaged on d 9 to induce coccidiosis in chickens. Performance parameters were evaluated during starter, grower, and finisher phases, and 4 birds per pen were euthanized on d 16 for sampling, FPD was scored on d 35, and litter moisture was analyzed on d 17 and 35. The data were analyzed using 1-way ANOVA with Tukey's test to separate means, and Kruskal-Wallis test was used for non-normally distributed parameters. The results showed that the Eimeria challenge was successful based on reduced weight gain and feed intake during grower phase, and higher FITC-d concentration, lesion score (female), and oocyst counts (d 14) in CC group compared to N.C. group, while XG and 2XG increased (P < 0.001) weight gain and improved FCR compared to CC and NC groups during finisher phase. The addition of X.G. and 2XG decreased litter moisture (P=0.003) and FPD (P < 0.001) in challenged broilers compared to the N.C. group (d 35). Supplementing XG and 2XG reestablished the population of Lactobacillus in the cecum of challenged birds to an intermediate level between the NC and CC groups (P > 0.05). The inclusion of XG tended to increase the expression of Junctional adhesion molecule 2 (JAM2), which was not different from CC and NC groups (P > 0.05). In conclusion, the combination of xylanase and glucanase (Natugrain TS) improved the performance and modulated jejunal microbiota of broilers under mild Eimeria challenge.

A role for Penicillium rubens strain 212 xylanolytic system in biocontrol of Fusarium wilt disease in tomato plants

Penicillium rubens strain 212 (PO212) is an effective inducer of resistance mechanisms in tomato plants against Fusarium oxysporum f.sp. lycopersici. During the interaction of PO212 with the plant, different classes of molecules may act as elicitors such as certain secreted endo-xylanases. The aim of this work was to elucidate the possible role of the xylanolytic system of PO212 in its biocontrol activity. We identified potential genes coding for xylanases (xlnA, xlnE, xylP), β-xylosidase (xlnD) and their transcriptional regulators (xlnR and araR) in PO212, and evaluated their transcriptional patterns in response to tomato root extracts or synthetic medium containing xylan as main carbon source. For this work we compared data from biocontrol strain PO212 with those of two strains of Penicillium, P. rubens S27, and P. chrysogenum IPLA33001, lacking the biocontrol efficacy. Time-course experiments showed the effect of these carbon sources on the expression rates of xylanase genes. To achieve a strong reduction in expression of xylanolytic genes, we generated a null allele of XlnR, as the specific regulator of the xylanase pathway. Absence of XlnR function prevented growth of PO212 on media containing xylan as main carbon source and consequently, expression levels of xylanases were downregulated. The relationship of the xlnR gene regulated pathway to the efficacy of PO212 as a biological control agent was evaluated. Null xlnR strains did not reduce either disease severity or incidence as wild-type PO212 does. Thus, there is a relationship between a correct expression of xylanolytic system and the capability of these enzymes as effective elicitors to trigger of plant defense systems in tomato plants against F. oxysporum f.sp. lycopersici.

Enhanced rice plant (BRRI-28) growth at lower doses of urea caused by diazinon mineralizing endophytic bacterial consortia and explorations of relevant regulatory genes in a Klebsiella sp. strain HSTU-F2D4R

Endophytic biostimulant with pesticide bioremediation activities may reduce agrochemicals application in rice cultivation. The present study evaluates diazinon-degrading endophytic bacteria, isolated from rice plants grown in the fields with pesticide amalgamation, leading to increased productivity in high-yielding rice plants. These endophytes showed capabilities of decomposing diazinon, confirmed by FT-IR spectra analysis. Growth promoting activities of these endophytes can be attributed to their abilities to produce an increased level of IAA content and to demonstrate high level ACC-deaminase activities. Furthermore, these endophytes demonstrated enhanced level of extracellular cellulase, xylanase, amylase, protease and lignin degrading activities. Five genera including Enterobacter, Pantoea, Shigella, Acinetobacter, and Serratia, are represented only by the leaves, while four genera such as Enterobacter, Escherichia, Kosakonia, and Pseudomonas are represented only by the shoots. Five genera including, Klebsiella, Enterobacter, Pseudomonas, Burkholderia, and Bacillus are represented only by the roots of rice plants. All these strains demonstrated cell wall hydrolytic enzyme activities, except pectinase. All treatments, either individual strains or consortia of strains, enhanced rice plant growth at germination, seedling, vegetative and reproductive stages. Among four (I-IV) consortia, consortium-III generated the maximum rice yield under 70% lower doses of urea compared to that of control (treated with only fertilizer). The decoded genome of Klebsiella sp. HSTU-F2D4R revealed nif-cluster, chemotaxis, phosphates, biofilm formation, and organophosphorus insecticide-degrading genes. Sufficient insecticide-degrading proteins belonging to strain HSTU-F2D4R had interacted with diazinon, confirmed in molecular docking and formed potential catalytic triads, suggesting the strains have bioremediation potential with biofertilizer applications in rice cultivation.

Influence of light regulation on growth and enzyme production in rare endolichenic fungi.

The influence of light regulation on the growth and enzyme production of three endolichenic fungal isolates, i.e. Pseudopestalotiopsis theae (EF13), Fusarium solani (EF5), and Xylaria venustula (PH22), was determined. The isolates were exposed to blue, red, green, yellow, white fluorescent light (12h light-12h dark photoperiod) (test), and 24h dark (control) conditions. Results revealed that the alternating light-dark conditions resulted in the formation of dark rings in most fungal isolates but was absent in PH22. Red light induced sporulation while yellow light elicited higher biomass in all isolates (0.19 ± 0.01g, 0.07 ± 0.00g, and 0.11 ± 0.00g, for EF13, PH22, and EF5, respectively) as compared to incubation in the dark. Results also showed that blue light induced higher amylase activity in PH22 (15.31 ± 0.45 U/mL) and L-asparaginase activity in all isolates (0.45 ± 0.01 U/mL, 0.55 ± 0.39 U/mL, and 0.38 ± 0.01 U/mL, for EF13, PH22, and EF5, respectively) compared to both control conditions. Green light enhanced the production of xylanase (6.57 ± 0.42 U/mL, 10.64 ± 0.12 U/mL, and 7.55 ± 0.56 U/mL for EF13, PH22, and EF5, respectively) and cellulase (6.49 ± 0.48 U/mL, 9.57 ± 0.25 U/mL, and 7.28 ± 0.63 U/mL, for EF13, PH22, and EF5, respectively). In contrast, red light was the least effective light treatment as production of enzymes was the least, with lower levels of amylase, cellulase, xylanase, and L-asparaginase detected. To conclude, all three endolichenic fungi are light-responsive, with fungal growth regulated with the use of red light and yellow light, and manipulation of enzyme production via blue and green light.

Xylanase supplementation of pelleted wheat-based diets increases growth efficiency and apparent metabolizable energy and decreases viscosity of intestinal contents in broilers

This study was designed to test graded supplementation of a thermostable xylanase in pelleted, wheat-based diets fed to broiler chickens over a 28-d period. A total of 600 Ross 708 male broilers were allotted to 1 of 5 dietary treatments: positive control (PC), negative control (NC; 125 kcal of AME/kg diet reduction relative to PC), and NC supplemented with 10, 15, or 30 g/ton of xylanase. Wheat-soybean meal-based diets were pelleted and fed in 2 feeding phases (14-d each). Study outcomes included growth performance, AME, and ileal digesta viscosity with 20 battery cages of 6 birds per treatment. Data were analyzed by 1-way ANOVA along with estimation of Pearson correlation coefficients. Whereas no difference between NC and PC was observed for BW gain, NC birds exhibited increased (P < 0.05) feed intake during each feeding phase and overall, which caused improvements (P < 0.05) in feed conversion ratio (FCR) for PC vs. NC birds. The analyzed AME of PC birds was 112 kcal/kg of diet greater (P < 0.05) than for NC birds, though no differences in digesta viscosity were observed. Xylanase supplementation of the NC diet at 15 or 30 g/ton elicited overall improvements (P < 0.05) in BW gain beyond the PC, while the 30 g/ton level equalized feed intake with the PC. Regardless of level, xylanase supplementation improved (P < 0.05) the FCR relative to the NC, thereby equalizing the response with the PC. Similarly, supplementation with any xylanase level increased (P < 0.05) AME over the NC, making all treatments synonymous with the PC. Digesta viscosity of all xylanase-supplemented treatments was decreased relative to both the NC and PC treatments. Overall, this study provided clear evidence that addition of a thermostable xylanase to pelleted wheat-based diets elicited improvements in growth performance of broilers concomitant with a reduction in digesta viscosity and elevation of analyzed dietary AME content.

170 Improving Nutrient Value of Soybean Meal Using Characterized Novel Microbial Fermentation

Abstract Soybean meal (SBM) is a common plant-based protein source in the swine industry. Its crude protein content is 44 to 50%. However, the presence of antinutritional factors such as high molecular weight, difficult to digest proteins, phytate and non-starch polysaccharides (NSP) limit its use in pigs. We aimed to use fermentation approach to improve the nutrient value of SBM. We have screened, from different sources, and identified bacterial strains that secrete cellulase, xylanase and proteinase as candidates for feedstuff fermentation. Further studies revealed that a few of them are capable of breakdown glycinin and beta-conglycinin, the major proteins in SBM that are difficult to digest and often resulted in intestinal upset in monogastric animal. Molecular characterization revealed that isolate 9 is a Bacillus subtilis strain (B. subtilis 9) secreted high level of cellulase, xylanase, and proteinase comparing to the ATCC control strain and other isolates. It grew significantly faster than the control strain. In addition, it also survived a wide range of concentrations salts and pH. When used for SBM fermentation, protein profiling of fermented SBM using SDS-PAGE revealed a significant reduction of high molecular weight proteins, especially those of glycinin and beta-conglycinin, to small peptides. On a dry matter basis, neutral detergent fiber (NDF) of the fermented SBM was decreased by 34%; crude protein content was increased from 49 to 57%. Pigs fed with a diet formulated with the fermented SBM as the sole source of crude protein had significantly higher apparent ileal digestibility (AID) of dry matter (80 vs. 72 %), ash (56 vs. 36%), crude protein (84 vs. 78%), NDF (71 vs. 66%), and acid detergent fiber (62 vs. 53%) compared with the control pigs fed with unfermented soybean meal. In addition, we have recently explored the possibility of improving feed stuff fermentation efficiency combining microbial species, the effect of this approach will be discussed.

Effects of a combination of fibrolytic and amylolytic enzymes on ruminal enzyme activities, bacterial diversity, blood profile and milk production in dairy cows

We hypothesised that adding a combination of fibrolytic and amylolytic enzymes to the diet of early-lactation dairy cows would improve rumen enzyme activity and bacterial diversity, promote energy metabolism, and benefit milk production in cows. Twenty multiparous early-lactation (90 ± 5 d) Holstein cows with similar body conditions were randomly allocated to control (CON, n = 10) and experimental (EXP, n = 10) groups in a completely randomised single-factor design. The CON was fed only a basal total mixed ration diet, and the diet of the EXP was supplemented with a combination of fibrolytic and amylolytic enzymes at 70 g/cow/d (cellulase 3 500 CU/g, xylanase 2 000 XU/g, β-glucanase 17 500 GU/g, and amylase 37 000 AU/g). The experiment lasted 28 days, with 21 days for adaptation and 7 days for sampling. Enzyme addition increased the activity levels of α-amylase and xylanase, and the ammonia-N concentration (P < 0.05) tended to increase the activity of β-glucanase (P = 0.08) in rumen fluid. However, there was no significant difference in the rumen bacterial richness and diversity, phylum (richness > 0.1%) or genus (richness > 1%) composition between the CON and EXP groups (P > 0.05). A tendency of difference was found between CON and EXP (R = 0.22, P = 0.098) in principal component analysis. Ten genera showed different abundances across the CON and EXP groups (linear discriminant analysis effect size, linear discriminant analysis > 2). EXP increased the ratio of albumin to globulin and the concentrations of total cholesterol and low-density lipoprotein cholesterol (P < 0.05) and tended to increase triglycerides (P = 0.09) in blood. Milk yield, 3.5% fat-corrected milk yield and energy-corrected milk yield increased with enzyme supplementation (P < 0.05). The production levels of milk fat and lactose increased, but the percentage of solids, not fat and protein, decreased in EXP (P < 0.05). Although the DM intake was not affected, the feed efficiency tended to increase (P = 0.07) in EXP. In conclusion, dietary supplementation with a mixture of fibrolytic and amylolytic enzymes on multiparous early-lactation dairy cows increased α-amylase and xylanase activity levels in rumen fluid, enhanced milk performance and tended to improve the feed efficiency in cows.

Xylanase Supplementation in Wheat-Based Diets of Laying Hens Affects the Egg Yolk Color, Carotenoid and Fatty Acid Profiles.

Wheat is rich in non-starch polysaccharides (NSP) and their degradation in poultry diets is promoted by exogenous carbohydrases. The objective here was to evaluate the effect of adding an intrinsically thermostable xylanase on wheat-based diets for laying hens in yolk color, carotenoid and fatty acid profiles of eggs. A total of 128 laying hens were used for 12 weeks. They were randomly allocated to four dietary treatments with different levels of xylanase: T1: control (no xylanase), T2: 30,000 U/g, T3: 45,000 U/g and T4: 90,000 U/g, with 32 birds, 16 replicates per treatment (2 birds/replicate). At the end of the experimental period, egg yolk color index, redness (a*) and yellowness (b*) of egg yolks were found significantly higher in all the enzyme supplemented diet groups (T2, T3, T4) compared with the control (T1). Canthaxanthin levels were significantly higher in T3 than T1 (p < 0.05). Total n-3, n-6 and total polyunsaturated fatty acids (FAs) were significantly higher in T4 compared with the control (p < 0.01), while the reverse trend was evidenced for monounsaturated FAs. Additionally, total n-3 FAs were higher in the T2 than T1 (p < 0.005). Overall, the results showed that exogenous xylanase enzyme supplementation in wheat-based diets for laying hens contribute to maintaining egg yolk color. Overall, exogenous xylanase enzyme supplemented at all levels in wheat-based laying hens’ diets improved egg yolk color compared to the control diet. The enzyme supplemented at the higher level (90,000 U/g) improved polyunsaturated and reduced monounsaturated egg yolk fatty acid content.

Effects of exogenous xylanase supplementation in plant-based diets on growth performance, nutrient digestibility and digestive enzyme activities of juvenile mori (Cirrhinus mrigala)

The present experimental study was conducted to determine the effects of xylanase supplementation on growth performance, nutrient digestibility and digestive enzyme activities of mori, Cirrhinus mrigala. Mori with an initial body weight of 8.34 ± 0.23 g were fed seven experimental diets: a diet with high fish meal content (positive control, PC), a diet with high plant content (negative control, NC), and five other diets with increasing levels of xylanase (680, 1273, 1882, 2486 and 3091 U/kg). Each diet was fed to triplicate groups of 15 fish each for 60 days. After the trial termination, supplementation of xylanase significantly improved final weight (FW), weight gain (WG) and specific growth rate (SGR) compared to NC diet. The best feed conversion ratio (FCR) was observed at 1882 U/kg of xylanase supplementation level which was non-significant (P > 0.05) to PC diet (high fish meal content). Whole body proximate composition and apparent digestibility coefficient (ADC%) for dry matter (DM), crude protein (CP), crude fat (CF), crude ash (CA) and gross energy (GE) were significantly affected by xylanase supplementation. Activities of hepatopancreatic and intestinal amylase, protease and lipase showed an increasing trend with the increase in xylanase supplementation level, peaking at 1882 U/kg of xylanase (non-significant (P > 0.05) to PC diet) and declining thereafter (2486 U/kg-3091 U/kg). Quadratic regression analysis showed that 1948 U/kg of xylanase was the optimum value for supplementation of xylanase in plant-based diet. In conclusion, exogenous xylanase supplementation improved the growth performance, nutrient digestibility and digestive enzyme activities in juvenile mori fed plant-based diet.

New Intrinsically Thermostable Xylanase Improves Broilers’ Growth Performance, Organ Weights, and Affects Intestinal Viscosity and pH

Exogenous carbohydrases are commonly added to monogastric animal feed to degrade non-starch polysaccharides (NSP) to improve zootechnical performance and nutrient digestion. In the current study, the effects of dietary supplementation of a new, intrinsically thermostable, monocomponent xylanase on intestinal parameters, zootechnical performance and carcass traits of broilers were evaluated. A total of 720 1-day-old broilers were randomly allotted to four wheat-based diets with different levels of xylanase (T1: control; T2: 30,000 U/g; T3: 45,000 U/g; T4: 90,000 U/g) and fed from 1 to 35 days of age. The data showed that xylanase supplementation reduced the intestinal viscosity significantly, especially in the ileum. Duodenum and cecum pH was not influenced by xylanase supplementation, while T3 treatment showed the lowest pH value in jejunum and ileum. Xylanase supplementation reduced significantly feed conversion ratio (FCR) without affecting feed intake (FI). T2 treatment exhibited a higher body weight gain (BWG) compared the other treatments. Live weight, carcass weight, legs, and liver weight were significantly higher in T3 and T4 treatments compared to T1. No significant differences on foot pad lesions were observed among the four treatments. In conclusion, dietary supplementation of this new xylanase significantly improves intestinal tract viscosity and affects beneficially broilers’ performance and carcass traits.

Production of Cocktail Enzymes by Three Cladosporium Isolates and Bioconversion of Orange Peel Wastes into Valuable Enzymes

The current research demonstrates the biotechnological economization of accumulated and inefficiently used agro-industrial orange peel wastes to generate amylase, endoglucanase, exoglucanase, pectinase, and xylanase, industrially essential enzymes with growing demands in enzyme markets, from three Cladosporium isolates. In submerged fermentation (SmF) at 10°C, the isolate AUMC 10865 produced the highest level of amylase (4164 IU/gram dry substrate). Endoglucanase, exoglucanase and xylanase had development peaks (923 IU/gds, 2280 IU/gds, and 1646 IU/gds, respectively in case of Cladosporium sp. AUMC 11366. Pectinase produced the most (7840 IU/gds) in the strain AUMC 11340. At 30°C, the strain AUMC 11340 secretes the most amylase (4120 IU/gds), endoglucanase (2700 IU/gds) and xylanase (3220 IU/gds). Exoglucanase development reached the peak (8750 IU/gds) in the isolate AUMC 10865. The overall production (5570 IU/gds) was instead enhanced by pectinase in the AUMC 11366 isolate. In solid-state fermentation (SSF) at 10°C, the isolate AUMC 10865 outperformed the other two isolates producing 640.0 IU/gds amylase, 763.3 IU/gds endoglucanase, 771.0 IU/gds exoglucanase, 1273.23 IU/gds pectinase and 1062.0 IU/gds xylanase, while the isolate AUMC 11366 produced the least amount of 399.7 IU/gds, 410.0 IU/gds, 413.3 IU/gds, 558.7 IU/gds, and 548.0 IU/gds, respectively. At 30°C, the isolate AUMC 11340 was superiorly producing higher levels of amylase (973.3 IU/gds), endoglucanase (746.0 IU/gds), exoglucanase (1052.0 IU/gds), pectinase (1685.3 IU/gds) and xylanase (1340.0 IU/gds), whereas isolate AUMC 10865 generated the least amounts of amylase (556.7 IU/gds) and exoglucanase (452.7 IU/gfs), and the isolate AUMC 11366 produced the least endoglucanase (256.3 IU/gds), pectinase (857.7 IU/gfs) and xylanase (436.3 IU/gds) amounts.

PSVII-2 Effects of xylanase and its optimal supplementation level on viscosity of jejunal digesta, nutrient digestibility, intestinal health, and growth performance of nursery pigs

Abstract This study aimed to determine supplemental effects of xylanase (endo-β-1,4-xylanase, CJ BIO, Korea) and its optimal supplementation level on viscosity of jejunal digesta, nutrient digestibility, intestinal health, and growth performance of pigs. Sixty weaned pigs (6.9 ± 0.8 kg BW) were randomly allotted to 5 treatments based on a RCBD with initial BW and sex as block and fed in 3 phases (P1/2/3 for 10/14/14 d, respectively). Dietary treatment were the supplementation levels of xylanase providing (0, 220, 440, 880, and 1,760 XU/kg feed). Titanium dioxide (0.4%) was added to P3 diets as an indigestible marker to measure AID. On d 38, all pigs were euthanized to collect ileal and jejunal digesta to measure AID and viscosity, respectively; jejunal mucosa and tissue to measure intestinal health parameters. Data were analyzed using SAS 9.4. Xylanase supplementation from 0 to 350 XU/kg increased (P &amp;lt; 0.05) ADG (596 to 746 g/d) during the last week, whereas there was no effect by xylanase on the overall growth performance. Increasing xylanase supplementation reduced (P &amp;lt; 0.05) digesta viscosity (1.91 to 1.48 mPa.s); increased (P &amp;lt; 0.05) the AID of EE (83.9 to 89.5%), NDF (52.9 to 56.9%) and ADF (35.3 to 39.3%); tended to reduce Cupriavidus (P = 0.073; 1.33 to 0.63%) and Megasphaera (P = 0.063; 1.26 to 0.23%); and tended to increase Succinivibrio (P = 0.076; 1.10 to 2.71%) and Pseudomonas (P = 0.060; 4.89 to 13.29%). Xylanase supplementation from 0 to 520 XU/kg reduced (P &amp;lt; 0.05) jejunal MDA (0.99 to 0.58 µmol/mg protein). In conclusion, xylanase supplementation showed benefits on intestinal health by reducing digesta viscosity, oxidative stress status, and harmful bacteria in the jejunal mucosa and by increasing the AID of nutrients. Xylanase supplementation at a range of 350 to 520 XU/kg feed provided the most benefits.

Effects of supplemental xylanase and xylooligosaccharides on production performance and gut health variables of broiler chickens

BackgroundThis study evaluated the effects of supplemental xylanase and xylooligosaccharides (XOS) in a corn-soybean meal (SBM)-based diet on growth performance and intestinal health of broilers. A total of 288 day-old chicks (Cobb 500) were allocated to 36 floor pens (8 birds/pen) equally in 9 dietary treatments in a 3 × 3 factorial arrangement. The treatments were combinations of 3 levels of xylanase (0, 0.005% and 0.01% Econase XT) and 3 levels of prebiotics (0, 0.005% and 0.01% XOS) added to basal mash diets formulated in three phases (starter, d 0–14; grower, d 15–28; finisher, d 29–42). The feed intake and body weights were recorded weekly. On d 42, ileal sections were collected for histomorphometric and gene expression analysis, and cecal content was collected for determining short-chain fatty acids (SCFA) and microbiota.ResultsXylanase linearly (P < 0.01) increased the average daily gain (ADG) in both the finisher and total period and the final body weight gain (FBWG, 2940 & 2932 vs. 2760 g) of broilers. XOS did not significantly increase either ADG or FBWG (P > 0.05). Supplemental xylanase and XOS did not affect average daily feed intake and feed conversion ratio (P > 0.05). Xylanase and XOS did not change villus height (VH) or crypt depth (CD) ratio (P > 0.05). However, xylanase exhibited a trend (P = 0.097) on VH:CD ratio. The inclusion of 0.01% XOS without xylanase increased the level of IL-10 (a marker of anti-inflammatory cytokine) and IL-4 (a T-cell differentiation cytokine) genes compared with control (P < 0.05). The acetate production was increased by xylanase (P < 0.01) and XOS (P < 0.05) without an additive effect. Xylanase increased total SCFA (P < 0.01) while XOS had a tendency to increase (P = 0.052). Alpha and beta diversity of microbiota among treatments were not different (P > 0.05). However, the mean proportion of family Ruminococcaceae was increased by the supplemental 0.01% xylanase (P < 0.01).ConclusionIt can be concluded that XOS can enhance cecal fermentation, while xylanase can increase the body weight gain along with the fermentation metabolites in the ceca of broilers fed the corn-SBM-based diet but the effects may not always translate into an improved mucosal absorptive capacity and a better feed efficiency.

Use of Wheat Straw for Value-Added Product Xylanase by Penicillium chrysogenum Strain A3 DSM105774.

The present work highlights the valorization of the bulky recalcitrant lignocellulose byproduct wheat straw (WS) for the enhanced production of value-added xylanase by the locally sourced novel Penicillium chrysogenum strain A3 DSM105774 for the first time. The optimized production of xylanase by submerged state of fermentation of WS was achieved using a three-step statistical and sequential approach: one factor at a time (OFAT), Plackett–Burman design (PBD), and Box Behnken design (BBD). Incubation temperature (30 °C), WS, and ammonium sulphate were the key determinants prompting xylanase production; inferred from OFAT. The WS concentration (%(w/v)), yeast extract concentration (%(w/v)), and initial pH of the production medium imposed significant effects (p ≤ 0.05) on the produced xylanase, realized from PBD. The predicted levels of WS concentration, initial pH of the production medium, and yeast extract concentration provoking the ultimate xylanase levels (53.7 U/mL) with an 8.95-fold enhancement, localized by the estimated ridge of the steepest ascent of the ridge analysis path, were 3.8% (w/v), 5.1, and 0.098% (w/v), respectively; 94.7% lab validation. The current data underpin the up-scaling of xylanase production using this eco-friendly, cheap, and robust methodology for the valorization of WS into the value-added product xylanase.

Response of Broiler Chicks to Low-Energy Diet Supplemented With Lecithin or Xylanase Enzyme

Two experiments were conducted to study the effect of adding soy-lecithin or xylanase to low-energy diet on broilers growth performance, nutrient digestibility, intestinal lipase enzyme activity and economical efficiency. A total number of 256 unsexed Cobb500 broiler chicks, a day old, were classified into two equal experiments, 128 chicks each (4 treatments × 4 replicates × 8chicks). In each experiment, chicks were divided into 4 groups as follows: - the 1st group fed basal diet and served as control which fed recommended energy diet, the 2nd group fed diet low (100 Kcal / kg diet) than recommendation (LED), the 3rd and 4th groups were fed 2nd diet added with 0.5g lecithin / kg diet or 1.0g lecithin / kg diet, respectively (EXP.1). While, chicks were fed 2nd diet supplemented with 0.1g xylanase / kg diet or 0.2g xylanase / kg diet, respectively (EXP.2). The growth trail was lasted at 35 days of age. The results revealed that, groups of LED + 0.5g lecithin (EXP.1) and LED + 0.2g xylanase (EXP.2) recorded significantly higher BWG and better FCR during overall period than control and LED groups. In both experiments, there is a gradual increase in EE% digestibility coefficient, intestinal lipase activity and economical efficiency by increasing the addition level of either soy-lecithin or xylanase. It could be concluded that enriching low-energy diet with either 0.5g lecithin / kg diet or 0.2g xylanase / kg diet can improve broilers growth performance, intestinal lipase activity and enhanced economical net profit.