Total Alkaloid Levels Research Articles

Drought stress enhances phytochemical, antioxidant, antidiabetic and anticancer properties in Pandanus tectorius

Global warming is responsible for increasing temperatures and reducing rainfall in many regions of the world. Due to insufficient rainfall or lack of water in the soil, plants experience a range of morphological and biochemical alterations when they are exposed to drought conditions. Interestingly, the cells of drought-stressed plants can serve as a potential source for discovering new drugs. These secondary metabolic products have important biological functions that determine how plants interact with the environment and help them accommodate alterations. Present research has shed light on the response of Pandanus tectorius induced drought stress at 8th and 16th days. The studies observed the levels of various secondary metabolites in plants under drought-induced stress. Among the secondary metabolites analyzed, total alkaloids exhibited the highest overall concentration at 26.19 ± 0.83a. Furthermore, the observations found that drought stress had a significant impact on the plant's secondary metabolism. This resulted in increased levels of total alkaloids, saponins, tannins, and phenolics compared to the control plant. Especially the concentration of phenolics showed the most significant increase, with a remarkable 64.99% rise observed at the 16th day of treatment. The concentration of phenolics reached (20.52 ± 0.181c) at this level. Moreover, compared to control, P. tectorius exhibits significant antioxidant, antidiabetic, anti-inflammatory, molecular docking, and anticancer effects against MDA-MB-231 human breast cancer cell line inhibitory concentration IC50 values (48.91 ± 0.95 μg/mL) at 16th day treated plants. Consequently, the positive responses of P. tectorius drought stress enhance secondary metabolites. This research focus on drought stress stimulus prompts an urge in the production of secondary metabolites in P. tectorius, leading to an increase in its anticancer and anti-diabetic properties and treatments for type-2 diabetes and against fighting for cancer with minimal side effects and helping prevent diseases.

Short-Term Liquid Nitrogen Storage of Pyrostegia venusta Embryos: Effects on Germination, Phenotypic and Biochemical Characteristics, and In Vitro Secondary Metabolite Production

In this study, short-term liquid nitrogen (LN) storage was used as a strategy to conserve Pyrostegia venusta embryos, and its effects on in vitro germination, phenotypic and biochemical characteristics, and in vitro secondary metabolite production were assessed. Embryos stored in LN for 1 and 7 days presented a higher germination rate and germination speed index compared to those of the control (non-cryostored embryos). Short-term LN storage also favored the phenotypic characteristics of seedlings. LN storage significantly affected the proteins (PTN), soluble sugar (SS) and reducing sugar (RS) contents, oxidative metabolism, and phenylalanine ammonia-lyase (PAL) activity, as well as the total phenolic compound, flavonoid, phytosterol, and alkaloid levels in seedlings regenerated from embryos cryostored for 7 days. Benzoic acid derivatives and flavonoids were observed in regenerated non-acclimatized seedlings. LN storage did not affect the survival rate or phenotypic characteristics of seedlings during acclimatization. Acclimatization promoted significant changes in PTN, SS and RS contents, oxidative metabolism, and PAL activity in seedlings from embryos cryostored for 7 days. Roots from acclimatized seedlings exhibited the highest phenolic, phytosterol, and total alkaloid levels. Differences in the chromatographic profiles of the acclimatized seedlings compared with the non-acclimatized seedlings were observed. LN storage can be an effective means of ex situ conservation of P. venusta genetic resources.

Alkaloid Accumulation and Distribution within the Capsules of Two Opium Poppy (Papaver somniferum L.) Varieties.

The goal of this study was to clarify the role of capsule size and morphology in the alkaloid yield of poppy. In 2023, two industrial varieties from large-scale cultivation were investigated. Three classes of capsule size (large, medium, and small) and four organelles (wall, placenta, disc, and thalamus) of the seedless capsule were studied for their mass proportions and alkaloid contents. In 'Meara', large capsules had 41% lower total alkaloid levels than smaller ones. In 'Morgana', there was no difference in total alkaloids between size groups, but large capsules had higher contents of codeine and thebaine. Among the four organelles, the wall represented the largest mass in both varieties (60-67%), while the disc and the thalamus gave the lowest proportions (below 9%). In the variety 'Meara', the highest alkaloid contents appeared in the wall (2.69% d.w.), followed by the placenta, and the other two parts. 'Morgana' accumulated the highest alkaloid content (3.72% d.w.) in the placenta. Morphine follows the trend of the total content, while codeine and thebaine may differ. Accurate information on the accumulation of alkaloids in the generative organs may contribute to increasing effectiveness in target-oriented breeding and optimization of cultivation with an appropriate choice of variety.

Integrated metabolomic and metagenomic strategies shed light on interactions among planting environments, rhizosphere microbiota, and metabolites of tobacco in Yunnan, China.

Changes in climatic factors and rhizosphere microbiota led plants to adjust their metabolic strategies for survival under adverse environmental conditions. Changes in plant metabolites can mediate crop growth and development and interact with rhizosphere microbiota of the plant rhizosphere. To understand the interactions among environmental factors, rhizosphere microbiota, and metabolites of tobacco, a study was conducted by using integrated metagenomic and metabolomic strategies at four typical representative tobacco planting sites in Yunnan, China. The results showed that the agronomical and biochemical traits were significantly affected by temperature, precipitation (PREP), soil pH, and altitude. Correlation analyses revealed a significant positive correlation of temperature with length, width, and area of the leaf, while PREP correlated with plant height and effective leaf numbers. Furthermore, total sugar and reducing sugar contents of baked leaves were significantly higher, while the total nitrogen and total alkaloid levels were lower in tobacco leaves at site with low PREP. A total of 770 metabolites were detected with the highest number of different abundant metabolites (DMs) at Chuxiong (CX) with low PREP as compared to the other three sites, in which secondary metabolites were more abundant in both leaves and roots of tobacco. A total of 8,479 species, belonging to 2,094 genera with 420 individual bins (including 13 higher-quality bins) harboring 851,209 CDSs were detected. The phyla levels of microorganisms such as Euryarchaeota, Myxococcota, and Deinococcota were significantly enriched at the CX site, while Pseudomonadota was enriched at the high-temperature site with good PREP. The correlation analyses showed that the metabolic compounds in low-PREP site samples were positively correlated with Diaminobutyricimonas, Nissabacter, Alloactinosynnema, and Catellatospora and negatively correlated with Amniculibacterium, Nordella, Noviherbaspirillum, and Limnobacter, suggesting that the recruitment of Diaminobutyricimonas, Nissabacter, Alloactinosynnema, and Catellatospora in the rhizosphere induces the production and accumulation of secondary metabolites (SMs) (e.g., nitrogen compounds, terpenoids, and phenolics) for increasing drought tolerance with an unknown mechanism. The results of this study may promote the production and application of microbial fertilizers and agents such as Diaminobutyricimonas and Alloactinosynnema to assemble synthetic microbiota community or using their gene resources for better cultivation of tobacco as well as other crops in drought environments.

Bioactive compounds contents of and antioxidant activity of Nectan-dra megapotamica (Lauraceae)

Nectandra megapotamica (Spreng.) Mez., Lauraceae known as cinnamon, is a species native to Brazil, widely used in folk medicine. The present project aimed to evaluate the antioxidant activity and determine the contents of phenols, flavonoids, tannins, and total alkaloids in extracts from the leaves and bark of a specimen of N. megapotamica that occurs in the southern region of the state of Mato Grosso do Sul. Crude ethanolic extracts obtained from leaves and bark were tested for antioxidant activity by DPPH and ABTS methods and dosage of phenols, flavonoids, tannins, and total alkaloids by spectrophotometry in the visible region. The extracts exhibited considerable antioxidant activity, particularly the leaf extract was the most active in both methods, yielding IC50 of 9.0 ± 0.18 and 14.29 ± 0.16 μg.mL-1, respectively. This extract showed higher levels of phenols, flavonoids, tannins, and total alkaloids. The results suggest that the antioxidant activity is related to the presence of phenolic compounds and alkaloids, known antioxidant compounds, quantified in this study.

Principal Component Analysis to Assess the Changes of Yield and Quality in Pinellia ternata at Different Stages after Brassinolide Treatments

Brassinolide (BR) is the "sixth class" plant hormone, which plays an important role in various physiological and biochemical processes of plants. The wide variety of functions of Pinellia ternata means that there is huge demand for it and thus it is in short supply. This paper mainly assessed the changes of yield and quality in P. ternata at different stages after BR treatments by principal component analysis, in order to improve the yield and quality of P. ternata and at the same time determine the best harvest time. The results showed that the tuber yield of P. ternata was significantly increased by BR treatments at different stages (except for the 15th day). After the 15th, 45th, 60th, 75th, 90th, and 105th day of treatments, the tuber yield of P. ternata reached peak values at 0.10 (0.65 g), 0.50 (1.97 g), 0.50 (1.98 g), 1.00 (2.37 g), 1.00 (2.84 g), and 2.00 mg/L (3.76 g) BR treatment, respectively. The optimal harvest time was the 75th day after 0.10, 0.50, and 1.00 mg/L BR treatments, which not only significantly improved the yield of P. ternata, but also retained high level of total alkaloids in the tubers (20.89, 5.37, and 13.44%) and bulbils (9.74, 20.42, and 13.62%), high total flavone content in the tubers (17.66, 16.26, and 12.74%) and bulbils (52.63, 12.79, and 38.69%), and high β-sitosterol content in the tubers (25.26, 16.65, and 0.62%) of P. ternata, compared with the control, respectively.

Antioxidant Capacity Test, Total Phenolic, Total Alkaloid, and Toxicity of Marigold Flower (Tagetes Erecta L.)

Antioxidants are a set of compounds that can fight the effects of reactive oxygen species (ROS) so as to eliminate their effects in the form of damage to macromolecules of carbohydrates, fats, proteins, and DNA chains. One source of antioxidants that are easily available and widely available is through herbal ingredients such as marigold flowers (Tagetes erecta L.). Marigold flowers are well known and used as a source of antioxidants, anti-inflammatory, antibacterial, diuretic and accelerate wound healing. This flower is often consumed as an edible flower and is also drunk in the form of tea. This study aims to look for the antioxidant capacity, total phenolic, total alkaloids, and toxicity of marigold flowers. Marigold flower extract is obtained by evaporating the results of methanol maceration from dried marigold flowers. Test antioxidant capacity with DPPH (2,2-diphenyl-1-picrilhydrazyl) using Blois method, total phenolic using Singleton and Rossi method, total alkaloid by method by Trivedi et al, and BSLT toxicity by Meyer method. The antioxidant capacity of marigold flowers is measured in IC50 as much as 74.5 μg/mL which includes active antioxidant levels. The total phenolic content of marigold flowers was obtained as much as 10,350.68 μg/mL. Total alkaloid levels were obtained as much as 13.05 μg/mL. The BSLT toxicity test in LC50 is 162.82 μg/mL which is categorized as moderate toxicity. From the results of this study, marigold flowers can be used as a source of antioxidants and have antimitotic properties.

Alkaloid Cytotoxic Test on Ethanol Extract from Itchy Leaves (Laportea decumana (Roxb.) Wedd.)

The Itchy leaf [Laportea decumana (Roxb.) Wedd] is a plant that is widely used as traditional medicine by Papuans as a pain reliever. The purpose of this study was to determine the total level of alkaloids in itchy leaf extract and to test the cytotoxic activity using the BSLT method. The research series began with the extraction stage with the maceration method using 96% ethanol, which tested the alkaloid level. The test was carried out using the gravimetric method and the cytotoxic test using the alkaloid level test results using the BSLT method on shrimp larvae (Artemia salina L). The results showed that itchy leaf extract contained a total alkaloid level of 6.46%. Cytotoxic testing using the BSLT method showed an LC50 of 54.33 ppm and was found to be toxic.

Microencapsulation of phenolic-enriched extract from cocoa pod husk (Theobroma cacao L.)

• Microencapsulated powders had significantly lower moisture content and water activity. • Maltodextrin retained higher levels of total phenolics, total flavonoids , and total alkaloids . • Maltodextrin and gum Arabic (8:2 w/w) achieved higher water soluble index and total saponins , and lower moisture content. • Maltodextrin and gum Arabic (8:2 w/w) was recommended to microencapsulate the CPH extract.

Comparative assessment of effect of malt with different bud length on prolactin in hyperprolactinemia rat

Malt is the mature fruit of Hordeum vulgare L. after germination and drying and has been applied for treatment female abnormal galactorrhea. Previous studies have showed total alkaloids in malt have anti-HPRL effect. However, total alkaloids of malt change with the growth cycle, and the specified levels of total alkaloids in different bud length of malt have not been decided. To determine the definitive level of total alkaloids in different buds of malt and the most suitable bud length for clinical application by comparing effects on hyperprolactinemia rat. During the budding of malt, the content of total alkaloids first increased and then decreased, and it peaked at a bud length of 0.75 cm. Treated the HPRL model rats with different buds of malt, the PRL level was decreased, the number of PRLpositive cells and the mRNA expression level in the pituitary were significantly declined, and the number of dopamine D1 and D2 receptors in the hypothalamus was increased. The above changes were most significant in 0.75 cm bud. These results suggest that in terms of the content of effective substance and the effects on HPRL model rats, a malt bud length of 0.75 cm is optimal for clinical application.

Biochemical alterations in healthy and infected stem bark of mango genotypes infected by black banded disease

In this study, the level of total sugars, total phenols and total alkaloids were studied in the healthy and infected stem bark of six genotypes. Biochemical analysis of healthy and infected bark of mango showed that there was considerable decrease in the reducing sugar, non-reducing sugar, total sugars and total alkaloids content of infected bark. While phenol content of the infected bark showed considerable increase as compared to the healthy bark. In the present study, the contents of total sugars, reducing sugars and non-reducing sugars were higher in healthy bark of all the genotypes and decreased in infected bark.

Field Survey of Ephedra Plants in Central Asia (1). Characterization of Ephedra equisetina, Ephedra intermedia, and Their Putative Hybrids Collected in the Zaravshan Mountains of Tajikistan.

Field surveys of Ephedra plants were conducted in the Zaravshan Mountains of Tajikistan. E. equisetina, E. intermedia, and their putative hybrids were collected. They were identified based on their phenotypes and their sequences of nuclear ribosomal DNA internal transcribed spacer 1 (ITS1) region. Sequencing and species-specific PCR analyses of their ITS1 sequences revealed six putative hybrids of E. equisetina and E. intermedia. The total ephedrine and pseudoephedrine content of most of the Ephedra samples collected in Tajikistan were higher than the 0.7% lower limit prescribed by the Japanese pharmacopoeia, 17th edition (JP17), and varied from 0.34 to 3.21% by dry weight. The total alkaloid level of E. intermedia (11E08-1) cultivated in Japan varied from 1.77 to 2.30% by dry weight, which was much higher than the 0.7% lower limit prescribed by JP17.

Examining the Potential Role of Foliar Chemistry in Imparting Potato Germplasm Tolerance to Potato Psyllid, Green Peach Aphid, and Zebra Chip Disease.

Long-term, sustainable management of zebra chip disease of potato, caused by 'Candidatus Liberibacter solanacearum' (Lso) and vectored by potato psyllids (Bactericera cockerelli Sulc [Hemiptera: Triozidae]), requires development of cultivars resistant or tolerant to infection or capable of reducing spread or both. We examined the influence that five experimental breeding clones of potato had on potato psyllids and their ability to vector Lso. The ability of these potato clones to resist aphids (green peach aphids, Myzus persicae Sulzer [Hemiptera: Aphididae]) also was examined. Due to the importance of host chemistry on plant-insect interactions, levels of primary metabolites of amino acids and sugars, as well as secondary metabolites including polyphenolics, terpenoids, and alkaloids were compared between breeding clones and a commercial cultivar. Findings for compound levels then were associated with observed changes in host susceptibility to psyllids or aphids. Psyllids oviposited less on three breeding clones than Atlantic, but no significant effects of breeding clones on psyllid feeding or choice were observed. Aphid reproduction was reduced on two clones relative to Atlantic. A05379-211 had greater sugar levels and postpsyllid amino acid levels than Atlantic. Total alkaloid and phenolic levels were greater in all breeding clones than Atlantic. Total terpenoid levels were greater in PALB03016-3 and PALB03016-6 than Atlantic, which might explain, in part, the observed resistance to psyllid oviposition and aphid reproduction. Overall, these results suggest that increased levels of certain metabolites in breeding clones could affect psyllid and aphid reproduction.

A preliminary investigation into the potential effect of Artemisia afra on growth and disease resistance in sub-adults of Oreochromis mossambicus

This study investigated the potential of Artemisia afra on growth and disease resistance in Oreochromis mossambicus. Phytochemical analysis of A. afra was also undertaken. Five A. afra-based diets designated as D1, D2, D3, D4 and D5 were formulated and randomly fed to triplicate groups of 45 fish (32.5±1g). At the end of feeding, Food Conversion Ratio and Specific Growth Rate values were not significantly different (F15,4=0.6, p>0.05; F15,4=0.45, p>0.05, respectively) between D1 and the other diets. Weight gain and feed intake were higher in D1, D2 and D3 than in D4 and D5, but no significant difference (ANOVA, p>0.05) was found between treatments. This suggests that safe dosages were used. Phagocytosis and lysozyme activity were higher in fish fed D4 and D5 than those fed D1 and the other diets. White blood cells were also higher in fish fed the A. afra-based diets. To test for disease resistance, fish from each dietary treatment were injected with varying concentrations (0, 1×106cfu, 1.5×106cfu, 3×106cfu, 4×106cfumL˗1) of Aeromonas hydrophila. Relative percentage survival and the survival rate of O. mossambicus were higher in fish fed D4 and D5, indicating that the use of A. afra-based diets enhanced immunity of O. mossambicus. This is because A. afra contained a number of biologically active compounds that act as immunostimulants. High levels of total polyphenol (70.32±3.5mg100g˗1), total phenols (88.65±5.32mg100g˗1), total flavonoids (181.5±4.13mgg˗1) and alkaloids (29.63±3.58gkg˗1) were recorded in A. afra.

Growth Inhibitory Effects of Adhatoda vasica and Its Potential at Reducing Listeria monocytogenes in Chicken Meat.

The inhibitory effects of Adhatoda vasica ethanolic leaf extract (AVELE) against Listeria monocytogenes were examined to assess its potential to preserve minimally processed meat products safely. The total phenolic, flavonoid, and alkaloid levels in AVELE were 10.09 ± 4.52 mg of gallic acid equivalents (GAE)/g, 22.43 ± 1.62 mg of quercetin equivalents/g, and 19.43 ± 3.90 mg/g, respectively. AVELE (1, 5, 10, or 20%) had considerable antibacterial effects against L. monocytogenes NCIM 24563 in terms of the inhibitory zones (7.4–13.6 mm), MIC (100 mg/mL or 10% formulated solution), reduced cell viability, potassium ion efflux, and the release of 260-nm absorbing materials and extracellular ATP. AVELE was used as a rinse solution (5, 10, and 20%) for raw chicken breast meat. A 20% rinsing solution applied for 60 min inhibited the L. monocytogenes NCIM 24563 counts significantly on raw chicken breast meat. Moreover, L. monocytogenes NCIM 24563 did not grow in the meat sample when the rinse time was increased to 90 min at the same concentration. L. monocytogenes showed a greater reduction to ~3 CFU/g after rinsing with a 10 and 20% AVELE solution for 30 min than with a 5% AVELE solution. The rinsing processes with AVELE produced the final cooked chicken products with higher sensory attribute scores, such as taste, juiciness, and tenderness, compared to the control group along with a decrease in microbial contamination. Chicken meat rinsed with AVELE (rinsing time of 90 min) showed better sensory attribute scores of juiciness and tenderness, as well as the overall sensory quality compared to the untreated group. This research highlights the effectiveness of AVELE against L. monocytogenes NCIM 24563, suggesting that AVELE can be used as an effective antimicrobial marinade and/or a rinse for meat preservation.

Pengaruh metode penyarian terhadap kadar alkaloid total daun jembirit (Tabernaemontana sphaerocarpa. BL) dengan metode spektrofotometri visibel

One of the plants which contain the alkaloid is a plant jembirit ( Tabernaemontana sphaerocarpa BL. ).Sap and leaves from this plants have been used to treat skin diseases and sprain. Alkaloid from jembirit plants showed potent cytotoxicity against various human cancer cell. The goal of this research is to find out the influence of the extraction method against the level of total alkaloid jembirit leaves.Jembirit leaves were extracted by maceration method and extraction with Soxhlet apparatus used ethanol 70 % as a solvent . Standardization of extracts conducted by test of ash content, test of drying shrinkage, and extract yield. Qualitative analysis conducted by alkaloid test. Determination of total alkaloid was analyzed with visible spectrophotometry method using Bromocresol green as complexing agent. The results showed that jembirit leaves contained alkaloid compounds. The determination resulted the levels of total alkaloid of maceration was 0.727% ± 0.0032, levels of total alkaloid extraction with Soxhlet apparatus was 0.666% ± 0.0022. The stastitical analysis showed significance differences of total alkaloid levels between maceration method and extraction with Soxhlet apparatus viewed from siginificancy value (0.001<0.005).

Assessment of nutritional quality of water hyacinth leaf protein concentrate

This study was embarked upon to convert water hyacinth, an environmental nuisance, to a natural resource for economic development. Water hyacinth leaf protein concentrate (WHLPC) was extracted in edible form and determination of its physicochemical characteristics, total alkaloids and phenolic compounds was done. Analysis of proximate composition and amino acid profile of the WHLPC was also done. The level of heavy metals (mg/kg) in WHLPC was found to be Cd (0.02±0.001), Cr (0.13±0.001), Pd (0.003±0.001) and Hg (0.02±0.001) while concentrations of Pb, Pt, Sn, Fe, Cu, Zn, Ni and Co were found to be 0.001±0.00. Level of all heavy metals was found to be within safe limit. Proximate analysis revealed that protein in WHLPC accounted for 50% of its nutrients, carbohydrate accounted for 33% of its nutrients while fat, ash and fibre made up the remaining nutrients. Amino acid analysis showed that WHLPC contained 17 out of 20 common amino acids, particularly, Phe (3.67%), Leu (5.01%). Level of total alkaloids and phenolic compounds was 16.6mg/kg and 6.0mg/kg respectively. Evidence from this study suggests that WHLPC is a good source of leaf protein concentrate (LPC); it is nutritious and acutely non toxic.

The effect of some soil parameters on the total alkaloid levels of tubers of Bongardia chrysogonum in three regions of Iran

The effect of some soil parameters on the total alkaloid levels of tubers of Bongardia chrysogonum in three regions of Iran

Tobacco nicotine uptake permease regulates the expression of a key transcription factor gene in the nicotine biosynthesis pathway.

The down-regulation of a tobacco (Nicotiana tabacum) plasma membrane-localized nicotine uptake permease, NUP1, was previously reported to reduce total alkaloid levels in tobacco plants. However, it was unclear how this nicotine transporter affected the biosynthesis of the alkaloid nicotine. When NUP1 expression was suppressed in cultured tobacco cells treated with jasmonate, which induces nicotine biosynthesis, the NICOTINE2-locus transcription factor gene ETHYLENE RESPONSE FACTOR189 (ERF189) and its target structural genes, which function in nicotine biosynthesis and transport, were strongly suppressed, resulting in decreased total alkaloid levels. Conversely, NUP1 overexpression had the opposite effect. In these experiments, the expression levels of the MYC2 transcription factor gene and its jasmonate-inducible target gene were not altered. Inhibiting tobacco alkaloid biosynthesis by suppressing the expression of genes encoding enzymes in the nicotine pathway did not affect the expression of ERF189 and other nicotine pathway genes, indicating that ERF189 is not regulated by cellular alkaloid levels. Suppressing the expression of jasmonate signaling components in cultured tobacco cells showed that NUP1 acts downstream of the CORONATINE INSENSITIVE1 receptor and MYC2, but upstream of ERF189. These results suggest that although jasmonate-activated expression of MYC2 induces the expression of both NUP1 and ERF189, expression of ERF189 may actually be mediated by NUP1. Furthermore, NUP1 overexpression in tobacco plants inhibited the long-range transport of nicotine from the roots to the aerial parts. Thus, NUP1 not only mediates the uptake of tobacco alkaloids into root cells, but also positively controls the expression of ERF189, a key gene in the biosynthesis of these alkaloids.

Evaluation of the Variation of the Contents of Anti-Nutrients and Nutrients in the Seeds of Legumes

It is assumed that the content of anti-nutrients in legumes seeds and find out the dependency among their content and the amount of important nutrients. The influence of the agri-environmental conditions on concentration of anti-nutrients was evaluated on the basis of the analysis of the material collected from the experiments carried out in the years 2010-2011, located in different regions of Poland. The analyses were performed in the Main Chemical Laboratory of IUNG-PIB Pulawy and in Laboratory of Research Centre for Cultivar Testing (COBORU) in Slupia Wielka, near Poznan. The obtained results indicate that the region of cultivation did not have a significant impact on the concentration of these substances in the seeds of faba bean. Seeds of fodder pea of Muza and Marych cultivars contain significantly less tannins than Roch and Wiato cultivars. In the case of faba bean, however, fewer of these compounds were found at white-flowering cultivars. The average content of total alkaloids in lupine was definitely greater in blue lupine than in yellow lupine. The location of cultivation of yellow lupine did not have an influence on the level of total alkaloids and gramine. Blue lupine collected much less alkaloids in the location of Central Poland and significantly more in the North and West of Poland.