The level of scientific literacy among students in Indonesia is still low. Teachers have attempted to improve it through the use of different teaching models, a variety of resources and technology, and incorporating a scientific approach in the learning process. Mobile-based interactive multimedia can be used as an alternative learning resource to improve students’ scientific literacy. This study aims to explore about about the potential of using mobile-based multimedia interactive to improve scientific literacy. This study employs a qualitative research with the survey as the data collection method. The population in this study were all high school students in Special Region of Yogyakarta Province, Indonesia. Meanwhile, the research sample are 10th grade students of SMA 1 Pengasih with a total number of 108 students who were selected randomly. The questionnaires distribution process was conducted online with using Google Forms after the researcher demonstrate various types of media that can be used for learning activities. The results of this study showed that the use of mobile-based interactive multimedia in learning has a great opportunity to be able to increase students' scientific literacy because in addition to high student interest, there are also many benefits that will be obtained by students, especially to make it easier for students to understand learning material.