Levels Of Polyunsaturated Fatty Acids Research Articles

Comparison of the Thermal Behavior and Chemical Composition of Milk Powders of Animal and Plant Origin

The present study aims to perform a comparative analysis of the chemical composition and thermal behavior of two distinct milk types, namely animal and plant-based. The thermal analysis revealed the presence of the following classes of compounds: hydrocarbons, heterocycles, aldehydes, ketones, amines and alcohols. All types of milk contain saturated fatty acids (SFAs), monounsaturated fatty acids (MUFAs) and polyunsaturated fatty acids (PUFAs), though the relative proportions of these vary depending on the specific milk type. Animal milk powders contain SFAs, including palmitic, stearic, and myristic acids, as well as moderate amounts of MUFAs, such as oleic and palmitoleic acids. They also contain lower PUFAs, including linoleic and alpha-linolenic acids. In contrast, plant-based milk powders, particularly soy milk powder, are rich in both linoleic and alpha-linolenic acids. Plant-based milk typically exhibits lower levels of SFAs and higher levels of MUFAs and PUFAs when compared to milk of animal origin. In conclusion, the fatty acid profiles of animal and plant-based milk powders reflect the different nutritional attributes and health implications associated with each. Thermal behavior analysis offers insights into the stability and potential flavor changes that may occur during processing and storage. The comparative analysis highlights significant differences in the chemical composition and thermal behavior of animal and plant-based milk powders.

Nutraceutical Prospects of Pumpkin Seeds: A Study on the Lipid Fraction Composition and Oxidative Stability Across Eleven Varieties

The oxidative stability of nutritive and bioactive lipids is essential for their functionality. This study evaluated the potential of lipid fractions from pumpkin seeds obtained from eleven high-performing cultivars of Cucurbita maxima Duchesne, C. pepo L., and C. moschata Duchesne cultivated in Poland, aiming to evaluate their stability for nutraceutical applications. This study investigated the intrinsic relationship between chemical composition and oxidative stability to identify cultivars with promising functional potential and commercial value. The fatty acid, sterol, and lipid antioxidant profiles were characterized using gas chromatography (GC), GC–mass spectrometry (GC-MS), and ultra-high-performance liquid chromatography (UPLC), respectively. Antiradical activity was assessed via the DPPH assay, and oxidative stability was evaluated using differential scanning calorimetry (DSC). The oils exhibited high levels of polyunsaturated fatty acids (PUFAs) (59.5–68.6%), with n-6/n-3 fatty acid ratios ranging from 66.5 to 211.6. The lipid extracts contained up to 97.1% Δ7-sterols, while key antioxidants included squalene (616.6–3092.0 mg/kg) and γ-tocopherol (54.1–423.6 mg/kg). Notably, the C. pepo cultivar ‘Moonshine’ was the least abundant in these bioactive compounds. The carotenoid content ranged from 5.7 to 19.4 mg/kg across the extracts. Among the studied cultivars, ‘Show Winner’ and ‘Pink Jumbo Banana’ (C. maxima) stood out as promising candidates for nutraceutical applications due to their elevated levels of tocopherols, carotenoids, and squalene. A moderate n-6/n-3 fatty acid ratio (100–170), coupled with balanced levels of γ-tocopherol and squalene, was found to significantly enhance the oxidative stability of pumpkin seed lipids. These lipid fractions also show potential as stabilizing additives for oils rich in α-linolenic acid but deficient in natural antioxidants.

Antioxidant Capacity, Lipid Oxidation, and Quality Traits of Slow- and Fast-Growing Meagre (Argyrosomus regius) Fillets During Cold Storage

Meagre (Argyrosomus regius) is an important species in aquaculture, with size and flesh quality playing key roles in its production and marketability. This study aimed to examine the relationship between growth and flesh quality parameters, including fatty acid content (FA), total antioxidant capacity (TOAC), superoxide dismutase (SOD), lipid oxidation (LO), muscle cellularity (MC), and filleting yield (FY) during cold storage. Fish from the same hatchery raised under identical conditions showed size variation after 12 months. Fish below 600 g were classified as slow growing (SG), while those above 1000 g were classified as fast-growing (FG). The results showed that FG fish had higher body weight, moisture, and FY but exhibited lower levels of fat and polyunsaturated fatty acids (PUFA). SG fish had higher TOAC and SOD activity, which significantly declined during cold storage in both groups but remained higher in SG fish. Despite the higher lipid content in SG fish, no significant differences in malondialdehyde (MDA) levels, an indicator of LO, were observed between the two groups, suggesting that the elevated antioxidant defenses in SG fish mitigated lipid peroxidation. This study underscores intrinsic antioxidants’ potential to preserve lipid quality of fish fillets during cold storage.

Exploration of Novel Metabolic Mechanisms Underlying Primary Biliary Cholangitis Using Hepatic Metabolomics, Lipidomics, and Proteomics Analysis.

Metabolic reprogramming is important in primary biliary cholangitis (PBC) development. However, studies investigating the metabolic signature within the liver of PBC patients are limited. In this study, liver biopsies from 31 PBC patients and 15 healthy controls were collected, and comprehensive metabolomics, lipidomics, and proteomics analysis were conducted to characterize the metabolic landscape in PBC. We observed distinct lipidome remodeling in PBC with increased polyunsaturated fatty acid levels and augmented fatty acid β-oxidation (FAO), evidenced by the increased acylcarnitine levels and upregulated expression of proteins involved in FAO. Notably, PBC patients exhibited an increase in glucose-6-phosphate (G6P) and purines, alongside a reduction in pyruvate, suggesting impaired glycolysis and increased purines biosynthesis in PBC. Additionally, the accumulation of bile acids as well as a decrease in branched chain amino acids and aromatic amino acids were observed in PBC liver. We also observed an aberrant upregulation of proteins associated with ductular reaction, apoptosis, and autophagy. In conclusion, our study highlighted substantial metabolic reprogramming in glycolysis, fatty acid metabolism, and purine biosynthesis, coupled with aberrant upregulation of proteins associated with apoptosis and autophagy in PBC patients. Targeting the specific metabolic reprogramming may offer potential targets for the therapeutic intervention of PBC.

Genetically Predicted Gene Expression Effects on Changes in Red Blood Cell and Plasma Polyunsaturated Fatty Acids.

Polyunsaturated fatty acids (PUFAs) including omega-3 and omega-6 are obtained from diet and can be measured objectively in plasma or red blood cells (RBCs) membrane biomarkers, representing different dietary exposure windows. In vivo conversion of omega-3 and omega-6 PUFAs from short- to long-chain counterparts occurs via a shared metabolic pathway involving fatty acid desaturases and elongase. This analysis leveraged genome-wide association study (GWAS) summary statistics for RBC and plasma PUFAs, along with expression quantitative trait loci (eQTL) to estimate tissue-specific genetically predicted gene expression effects for delta-5 desaturase (FADS1), delta-6 desaturase (FADS2), and elongase (ELOVL2) on changes in RBC and plasma biomarkers. Using colocalization, we identified shared variants associated with both increased gene expression and changes in RBC PUFA levels in relevant PUFA metabolism tissues (i.e., adipose, liver, muscle, and whole blood). We observed differences in RBC versus plasma PUFA levels for genetically predicted increase in FADS1 and FADS2 gene expression, primarily for omega-6 PUFAs linoleic acid (LA) and arachidonic acid (AA). The colocalization analysis identified rs102275 to be significantly associated with a 0.69% increase in total RBC membrane-bound LA levels (p = 5.4 × 10-12). Future PUFA genetic studies examining long-term PUFA biomarkers are needed to confirm our results.

ИССЛЕДОВАНИЕ СЕЗОННЫХ ИЗМЕНЕНИЙ ПРОФИЛЯ ЖИРНЫХ КИСЛОТ ПЛАЗМЫ КРОВИ КОРЕННЫХ ЖИТЕЛЕЙ ЯКУТИИ

The aim of this study was to investigate seasonal changes in the composition of plasma fatty acids in the blood of indigenous peoples of the North as a mechanism of adaptation to extreme climatic conditions. The object of the study were Yakut male students (n=26), who were examined in four seasons: autumn, winter, spring and summer. Written informed voluntary consent was obtained from each participant. The longitudinal study included the determination of the level of saturated (SFA), monounsaturated (MUFA) and polyunsaturated fatty acids (PUFA) in different seasons of the year using gas chromatography and mass spectrometry (GC-MS). The results were statistically processed on the MetaboAnalyst 5.0 web platform in order to identify seasonal changes in the metabolic profile. The study showed that seasonal changes in the composition of plasma fatty acids are an important mechanism of adaptation of the body of young Yakut men to the harsh conditions of the North. Autumn changes in the lipid profile ensure a smooth transition to the winter period and create the basis for successful adaptation of the body to cold stress. A decrease in the level of SFA in winter is associated with their active use to ensure thermoregulation and maintain energy balance, while an increase in the proportion of PUFA and MUFA helps maintain the structure and function of cell membranes in cold conditions. PUFA also play a key role in maintaining psychoemotional stability and cognitive functions, which is important for survival and normal functioning under winter stress. Seasonal changes in the fatty acid profile may be key elements in the adaptation of indigenous populations to harsh climatic conditions.key elements in the adaptation of indigenous populations to harsh climatic conditions.

COVID-19 Serostatus Does Not Affect the Intrauterine Transfer of Micronutrients and Fatty Acids or Maternal–fetal Lymphocyte Cell Composition: An Observational Study

Objective Studies on the effects of coronavirus disease 2019 (COVID-19) on pregnant mothers and their newborns, specifically in relation to their micronutrient status, fatty acids (FAs), and inflammatory status are sparse. We hypothesized that COVID-19 infection would adversely affect the transfer of nutrients, and FAs from mothers to their fetuses via the umbilical cord and maternal–fetal distribution of inflammatory cells. This study aimed to determine the effect of COVID-19 on micronutrients, inflammatory markers, and FAs profiles in pregnant mothers and their newborns' cord blood. Study Design This was a cross-sectional study of 212 pregnant mothers in the third trimester and their newborns, recruited after testing for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) serostatus. Peripheral blood of mothers and cord blood were collected at birth and analyzed for vitamin B12 (Vit B12), folic acid, 25(OH)D3, FAs, and peripheral blood mononuclear cells. Student's t-test or analysis of variance (ANOVA) was used to express statistical significance. Non-normal data were tested using the Mann–Whitney U test and Kruskal–Wallis test, with proportions compared with the chi-square test. Results Vit B12 levels were significantly low and adrenic acid levels significantly high in COVID-19 seropositive mothers while 25(OH)D3 was significantly low in seropositive cord blood. Irrespective of COVID-19 serostatus, folate, vit B12, saturated FA levels were significantly high in cord blood indicating their increased transfer from mothers to the fetus. However, monounsaturated (MUFA) and polyunsaturated fatty acid (PUFA) levels were significantly lower in cord blood. Irrespective of COVID-19 serostatus, CD4+ T helper cells (percentage of lymphocytes) were significantly higher in cord blood, while NK cells, NK-T cells, and CD8+ T-cytotoxic cells—percentage of lymphocytes—were significantly lower in cord blood when compared with corresponding mother's blood. Conclusion The results indicate that while COVID-19 did not impede the transfer of essential nutrients such as MUFA and PUFA from mother to fetus, or affect maternal–fetal immune cell responses, it did appear to affect the levels of vit B12, vitamin D, and adrenic acid. Key Points

Plasma n-3 polyunsaturated fatty acid levels and colorectal cancer risk in the UK Biobank: Evidence of non-linearity, as well as tumour site- and sex-specificity.

The relationship between n-3 polyunsaturated fatty acid (PUFA) intake and colorectal cancer (CRC) risk is unclear. Blood n-3 PUFA concentration is a biomarker of dietary n-3 PUFA intake. We examined the relationship between plasma n-3 PUFA concentrations and CRC risk in UK Biobank (UKBB) participants. We analysed the relationship between tertiles (T) of plasma total n-3 PUFAs and n-3 PUFA docosahexaenoic acid (DHA) levels, and overall CRC (also stratified by tumour location and sex) risk. Cox proportional hazards regression models were adjusted for clinical co-variates. Non-linearity was tested by restricted cubic splines. There were 2,602 incident CRC cases in 234,598 UKBB participants with baseline plasma fatty acid data (mean follow-up 13.4 years). There was an inverse association between the plasma total n-3 PUFA level (T2 hazard ratio [HR] 0.88[95% confidence interval 0.80-0.97] compared with the T1 reference; T3 0.91[0.83-1.00]), as well as the plasma DHA concentration (T2 0.89[0.80-0.98]; T3 0.91[0.82-1.00]), and CRC risk. The relationship was non-linear (P for non-linearity=0.14 [total n-3 PUFAs] and 0.008 [DHA]), with a plateau at the highest n-3 PUFA concentrations. The relationship was more pronounced for proximal colon cancer (T2 0.82[0.69-0.97], T3 0.76[0.64-0.90] for DHA) and was evident for males (T2 0.84[0.74-0.95], T3 0.89[0.78-1.00]), but not females. Higher plasma n-3 PUFAs are associated with reduced CRC risk in the UKBB. Non-linearity, tumour site- and sex-specificity of the inverse relationship between plasma n-3 PUFA levels and CRC risk, if confirmed in other diverse populations, have significant implications for nutritional prevention guidelines.

Feeding citrus pomace fermented with combined probiotics improves growth performance, meat quality, fatty acid profile, and antioxidant capacity in yellow-feathered broilers.

The reasonable and efficient utilization of agricultural by-products as animal feed has the capacity to not only mitigate the scarcity of conventional feedstuff but also alleviate the environmental load. This study was aimed to investigate the effects of feeding citrus pomace (CP) fermented with combined probiotics on growth performance, carcass traits, meat quality and antioxidant capacity in yellow-feathered broilers. A cohort of 540 female yellow-feathered broilers (Qingyuan partridge chicken, 90-day-old) were randomly divided into three groups and, respectively, fed the basal diet (Control), diet containing 10% unfermented CP (UFCP) and diet containing 10% fermented CP (FCP). The results showed that dietary FCP significantly increased (p < 0.05) the final-body-weight and average-daily-gain of broilers, and the pH45 min and b*24 h values in breast muscle, while tendentiously lowering the feed-to-gain ratio (p = 0.076). The levels of inosine monophosphate (p < 0.05) and intramuscular fat (p = 0.083) in the FCP group were higher than those in the control group. Remarkably, dietary FCP and UFCP increased the levels of polyunsaturated fatty acids (PUFAs) and n-6 PUFAs (p < 0.05). Moreover, dietary FCP decreased (p < 0.05) the malondialdehyde content and increased (p < 0.05) the glutathione peroxidase content in serum. Ingestion of FCP and UFCP increased the levels of total antioxidant capacity and catalase activity in serum, and concentrations of glutathione peroxidase and catalase in breast muscle (p < 0.05). Additionally, diet containing FCP or UFCP upregulated the expression of SREBP - 1c, FAS, NRF2, GSH-Px, and CAT in breast muscle (p < 0.05). Overall, dietary supplementation with FCP obviously improved meat quality, enhanced the antioxidant capacity and regulated the lipid metabolism, contributing to the improvement of growth performance of yellow-feathered broilers.

A comprehensive study of the fatty acid composition, thermal and some physicochemical properties of abdominal and subcutaneous fats of Linda, Mast and Turkish geese.

This study examined the chemical composition, including moisture content, total fat, iodine value, melting point, saponification number, differential scanning calorimetry, fatty acid profiles, and color values (L*, a*, b*), in abdominal (A) and subcutaneous (S) fat of 15-week-old geese from three female varieties: Turkish (Lo), Linda (Li), and Mast (Ma). Results showed significantly higher moisture content in Lo-S compared to abdominal fats (P < 0.05), with consistent fat contents among goose varieties in abdominal fats. Iodine value (IV), melting point (MP), and color values did not differ significantly across varieties within fat types (P > 0.05). The Ma-A group exhibited the lowest saponification number (SN). Lo-S, Li-S, and Ma-S displayed higher onset and peak temperatures than abdominal fats across all groups. Li-A exhibited the highest levels of polyunsaturated fatty acids (PUFA) and an optimal PUFA/SFA ratio (P < 0.05), and Li-S demonstrated the lowest Atherogenic Index (AI), supporting its health-promoting profile. Additionally, Ma-S showed elevated levels of unsaturated fatty acids (UFA), a high nutritive value index, and a favorable AI value (P < 0.05), indicating its suitability for heart-healthy diets. Principal Component Analysis (PCA) identified PC1 with associations to SFA, palmitic acid, iodine value, myristic acid, and a*, while PC2 emphasized total fat content and melting point as pivotal factors. These findings underscore distinct chemical profiles of abdominal and subcutaneous fats in different goose varieties, revealing variations in fatty acid composition and thermal properties. Overall, Li-S, Li-A, and Ma-S fats emerged as optimal choices for consumers seeking healthful dietary fats.

Comprehensive Evaluation of Nutritional, Physicochemical, and Volatile Profiles of Selected Bovine Head Muscles.

This study analyzed the nutritional composition, physicochemical properties, and volatile profiles of three major bovine head muscles-medial pterygoid, masseter, and buccinator-to reduce byproduct resource waste and increase the utilization rate of bovine head to establish a foundation for its industrial use. Compared to tenderloin, which is popular among consumers, these head muscles were found to be rich in collagen (4.90-13.1 mg/g), low in fat (0.39-1.61%), and abundant in free amino acids (143.93-223.00 mg/100 g). Their compact fiber structures, with minimal gaps between myocytes, resulted in lower cooking and press losses, making them suitable for various production processes. Notably, the medial pterygoid and masseter muscles contained high levels of polyunsaturated fatty acids (PUFAs) and lower saturated fatty acids (SFAs), with a PUFA/SFA ratio exceeding 0.45. The buccinator muscle, while containing more volatile organic compounds associated with undesirable odors and bitter amino acids, was not indicative of spoilage. Overall, this study confirmed that bovine head muscles possess high collagen, low fat, and diverse nutritional qualities, making them suitable as premium raw materials for value-added meat products, and their returns will be most economically equal to the meat derived from cattle.

Genetically predicted gene expression effects on changes in red blood cell and plasma polyunsaturated fatty acids.

Polyunsaturated fatty acids (PUFAs) including omega-3 and omega-6 are obtained from diet and can be measured objectively in plasma or red blood cells (RBCs) membrane biomarkers, representing different dietary exposure windows. In vivo conversion of omega-3 and omega-6 PUFAs from short-to long-chain counterparts occurs via a shared metabolic pathway involving fatty acid desaturases and elongase. This analysis leveraged genome-wide association study (GWAS) summary statistics for RBC and plasma PUFAs, along with expression quantitative trait loci (eQTL) to estimate tissue-specific genetically predicted gene expression effects for delta-5 desaturase ( FADS1 ), delta-6 desaturase ( FADS2 ), and elongase ( ELOVL2 ) on changes in RBC and plasma biomarkers. Using colocalization, we identified shared variants associated with both increased gene expression and changes in RBC PUFA levels in relevant PUFA metabolism tissues (i.e., adipose, liver, muscle, and whole blood). We observed differences in RBC versus plasma PUFA levels for genetically predicted increase in FADS1 and FADS2 gene expression, primarily for omega-6 PUFAs linoleic acid (LA) and arachidonic acid (AA). The colocalization analysis identified rs102275 to be significantly associated with a 0.69% increase in total RBC membrane-bound LA levels ( P =5.4×10 -12 ). Future PUFA genetic studies examining long-term PUFA biomarkers are needed to confirm our results.

Физиологические аспекты липидного обмена в условиях Арктической зоны Российской Федерации (обзор)

This article aimed to systematize data on lipid metabolism parameters in various population groups of the Arctic region in the historical aspect and in modern conditions. The materials were selected using international (Web of Science and Scopus) and Russian (Cyberleninka and eLIBRARY.RU) databases. The following keywords were searched for: Arctic, lipid metabolism, risk factors. The sample included 34 works on the changes in lipid metabolism in the inhabitants of the Arctic region published between 1979 and 2023. It has been shown that the indigenous population is more likely to have a favourable blood lipid profile, with a low content of lowdensity lipoproteins (LDL) and triglycerides (TG), but high content of high-density lipoproteins (HDL). In zerogeneration newcomers, when their adaptation is disrupted, atherogenic dyslipidaemia often develops, while in first- and second-generation newcomers, lipid metabolism occupies an intermediate position, approaching the parameters of the indigenous population. Along with climatic and geographical conditions, socio-economic factors (dietary changes, smoking and alcohol abuse) affect the human body, leading to atherogenic dyslipidaemia, which is the basis of cardiovascular diseases. An important role in assessing lipid metabolism disorders in the Arctic is played by determining more reliable markers of atherosclerosis, namely, the content of apolipoproteins (apoA and apoB) and fatty acids consumed. The indigenous inhabitants of the North typically have a protein- and fatrich diet characterized by increased levels of omega-3 and decreased levels of omega-6 polyunsaturated fatty acids (PUFAs). However, in recent decades, low levels of both omega-3 and omega-6 PUFAs have become more common. For early detection of signs of lipid metabolism disorders, we need to study not only the “traditional” parameters, i.e. total cholesterol, TG, LDL and HDL, but also apoA-I and apoB as well as omega-6 and omega-3 PUFAs in order to develop science-based diagnostic methods, differentiated wellness programmes and therapeutic measures.

Plasma Polyunsaturated Fatty Acid Levels and Mental Health in Middle-Aged and Elderly Adults.

Background: Polyunsaturated fatty acids (PUFAs) are promising nutrients for the prevention and management of psychiatric disorders. Both animal experiments and cohort studies have demonstrated the antidepressant effects of PUFAs, especially omega-3 PUFAs. However, inconsistent reports about specific types of PUFAs, such as the omega-3 and omega-6 PUFAs, still exist. Objectives: To assess the effects of specific PUFAs on mental disorders and related symptoms and explore the potential mechanisms involving white matter microstructure. Methods: Leveraging 102,252 residents from the UK Biobank, the effects of five PUFA measures on depressive disorder and anxiety disorder were explored through Cox regression models with full adjustment for possible confounders. Furthermore, the effects on related psychiatric symptoms and brain white matter microstructures were also estimated using logistic regression models and multiple linear regression models, respectively. Results: In this study, plasma levels of five PUFAs measured in quartile 4 were associated with lower risks of incident depressive disorder compared with the lowest quartile, with hazard ratios of 0.80 [95% confidence interval] = [0.71, 0.90] for total PUFAs, 0.86 [0.76, 0.97] for omega-3 PUFAs, 0.80 [0.71, 0.91] for docosahexaenoic acid, 0.79 [0.70, 0.89] for omega-6 PUFAs, and 0.77 [0.69, 0.87] for linoleic acid. Similar associations were observed between PUFAs and the incident risk of anxiety disorder. In addition, high plasma PUFA levels were also related to lower risks of occurrence of several adverse psychological symptoms, especially omega-3 PUFAs and DHA. Among the included participants, 8780 individuals with brain imaging information were included in further neuroimaging analyses, and significant associations with white matter microstructures were observed. Conclusions: Thus, this study provides population-based evidence to support the value of interventions to target PUFAs (specifically omega-3 PUFAs) for the prevention and improvement of mental health.

Characterization of meat quality, storage stability, flavor-related compounds, and their relationship in Korean Woorimatdag No. 2 chicken breast meat during cold storage

We evaluated the quality, storage stability, and flavor-related compounds of breast meat from a novel Korean native chicken breed (Woorimatdag No. 2; WRMD2) and commercial broiler (CB) during seven days of aerobic cold storage. We found that pH and drip loss increased gradually during storage and WRMD2 exhibited a significantly lower pH and higher drip loss than CB. In both groups, aerobic plate counts, volatile basic nitrogen, and lipid oxidation levels increased, whereas creatine and dipeptide levels gradually decreased during storage. WRMD2 exhibited a significantly higher anserine content and lower carnosine-to-anserine ratio than CB. Flavor nucleotide content was influenced more by the storage period, whereas fatty acid composition was affected more by genetic differences. WRMD2 exhibited significantly higher levels of polyunsaturated fatty acids, especially C20:4n6 and C22:6n3, than CB. Interestingly, multivariate analysis highlighted several volatile compounds, including methyl salicylate (day 1), dodecanal (day 3), naphthalene (day 5), and 2,4-decadienal (day 7) as potential biomarkers to distinguish between WRMD2 and CB on each storage day. Correlation analysis identified five key meat quality traits, including drip loss, aerobic plate counts, and anserine that are strongly associated with flavor substances, such as inosine monophosphate, guanosine monophosphate, 1-octen-3-ol, and hexanal. These results offer insights into the distinctive meat quality and flavor compounds in WRMD2 during storage, providing fundamental data that could improve the management and quality of native chicken meat.

Review of hemp components as functional feed and food ingredients: impact on animal product quality traits and nutritional value

This review examined the potential of hemp components as functional feed and food ingredients, focusing on their impact on the quality and nutritional value of animal products. Following hemp legalization, there was growing interest in its potential to enhance animal diets and processed animal products due to its rich nutritional profile, including high levels of polyunsaturated fatty acids (PUFA), essential amino acids, and fibre. Incorporating hemp components into feed for monogastric animals, particularly poultry, improved lipid stability, sensory attributes, and the fatty acid composition of meat and eggs. Hemp supplementation for ruminants, especially in goats, increased PUFA and conjugated linoleic acid (CLA) content in milk, improved meat tenderness, and enhanced oxidative stability. However, research on hemp supplementation for pigs and beef remained limited, indicating the need for further exploration of these species. Hemp cake, rich in protein, fibre, and essential fatty acids, was the most widely used hemp component due to its economic viability, nutritional benefits, and sustainability, contributing to improved meat and milk quality. Regulatory concerns about the transfer of tetrahydrocannabinol (THC) residues in the produced animal products restricted the use of hemp biomass. In processed animal products, hemp components were studied for their potential to enhance nutritional value, replace animal fats, and serve as natural preservatives. Although they improved the fatty acid profile and antioxidant properties of meat products, challenges such as textural changes and increased lipid oxidation needed to be addressed for optimal use. Limited studies on dairy products indicated promising nutritional enhancements, but textural issues could impact consumer acceptance. In conclusion, hemp components show significant potential for improving the quality and nutritional value of animal products. Further research is necessary to address regulatory, sensory, and formulation challenges and to expand their application across different animal species and processed animal products.

Relationship Between Serum ω-3 Polyunsaturated Fatty Acid Concentration and Fatty Acid Fraction of Epicardial Adipose Tissue in Patients With Cardiovascular Disease.

ω-3 polyunsaturated fatty acids (PUFAs), such as eicosapentaenoic acid (EPA, C20:5ω3) and docosahexaenoic acid (DHA, C22:6ω3), are widely regarded as cardioprotective. EPA, but not DHA, has been reported to prevent fibrosis in heart failure. The relationship between the ω-3 PUFA fraction in epicardial adipose tissue (EAT) and subcutaneous adipose tissue (SAT) and vascular inflammation in patients with cardiovascular disease remains unclear. EAT was collected from patients undergoing cardiovascular surgery (n=21, 11 men, 10 women, 70.4±9.0 years old). Fatty acid fractions were measured in serum, SAT, and EAT by gas chromatography, and serum tumor necrosis factor (TNF) α was measured. No patient had taken EPA or DHA supplements. DHA concentrations were significantly higher in EAT than in SAT (p=0.001); EPA showed no significant difference. The EPA level of EAT correlated with the serum EPA concentration (p=0.009)but did not correlate significantly with serum DHA, α-linolenic acid (ALA, C18:3ω3), or linoleic acid (LA, C18:2ω6) concentrations. The EPA level of EAT showed a strong correlation with docosapentaenoic acid (DPA, C22:5ω3) and the DHA level of EAT (DHA: p<0.001). ALA (r=-0.519, p=0.039) and EPA (r=-0.611, p=0.027) levels of EAT correlated negatively with the serum TNFα concentration. There were no significant differences in EPA in serum, SAT, and EAT between patients with and without atherosclerotic heart disease. Among ω-3 PUFA fractions in EAT, ALA and EPA were associated with anti-inflammatory effects in patients with cardiovascular disease. It is likely that an increase in serum EPA concentration is needed to increase ω-3 PUFA levels in EAT.

Effect of Phycocyanin and Butylated Hydroxytoluene on the Oxidative Stability of Safflower Oil: A Comprehensive Kinetic Investigation

ABSTRACTSafflower includes both saturated and unsaturated fatty acids especially high levels of polyunsaturated fatty acid (PUFA). The addition of antioxidants during oil processing is one of the most effective methods. The aim of this research was to evaluate the oxidative stability of safflower oil supplemented with phycocyanin (PC) (200, 300, and 400 ppm), or butylated hydroxytoluene (BHT) (200 ppm), at 80, 90, and 100°C during the storage time. Oxidative stability of oil was measured through the assay of primary and secondary oxidation products: Peroxide value (PV) and thiobarbituric acid reactive substances (TBARS) were evaluated, respectively. Results showed that the addition of PC at 300 and 400 ppm caused the lowest peroxide levels. In addition, the oxidation reactions of this oil followed a first‐order kinetic model for PV and TBARS. The amounts of PV and TBARS were dependent on the storage temperature; according to the Arrhenius equation, the activation energy (Ea) of the samples that contain 300 and 400 ppm PC decreased in all the tested temperatures compared to control. Results of the oxidative stability indicated that PC may have superior antioxidant properties than BHT and can potentially inhibit the oil oxidation. The sample containing 300 ppm PC demonstrated heightened efficacy in suppressing primary oxidation. Moreover, the results of sensory evaluation test showed that the 300 ppm PC sample received the highest rating for overall quality, which confirm that 300 ppm PC sample could be the best sample to decrease the oxidative stability in safflower oil.

Effects of n-3 polyunsaturated fatty acids and selenomethionine supplementation on physicochemical properties, oxidative stability and endogenous enzyme activities of fresh pork loin

This study evaluated the physicochemical properties, oxidative stability, and endogenous enzyme activities in fresh pork loin from pigs fattened by supplementation of 3 % soybean oil (control), 3 % linseed oil or 3 % linseed oil combined with 0.3 mg/kg selenomethionine (SeMet). Both linseed oil treatments led to higher n-3 polyunsaturated fatty acids (PUFA) levels, lower L* values, n-6/n-3 ratios, and lipoxygenase activity compared to the control (P < 0.001). Supplementation with linseed oil alone reduced a*, n-6 PUFA, saturated and monounsaturated fatty acids, and GPx activity in pork loin compared to other treatments (P < 0.05). Adding SeMet decreased neutral lipase activity but did not affect a* or GPx activity (P > 0.05), likely due to the antioxidant property of SeMet. Overall, linseed oil and SeMet supplementation is a promising strategy to significantly increase n-3 PUFA content in pork without compromising color properties or oxidative stability.

Associations among dietary intake, gene polymorphisms and adipose fatty acid in TwinsUK study

Fatty acids are essential molecules, which function as structural components, energy sources, and signaling mediators(1). Subcutaneous adipose tissue (SAT) is the largest fat depot and plays a crucial role in maintaining health and homeostasis(2,3). While regulation and health impacts of circulating fatty acid levels are well established, less is known about the regulation of fatty acid levels within adipose tissue itself. The aim of this study was to investigate the effect of dietary and genetic contribution to fatty acid contents in adipose tissue and identify the interaction between single nucleotide polymorphism (SNP) and diet on adipose fatty acids.In 427 healthy female twins from TwinsUK, 18 types of fatty acids were measured in SAT biopsies alongside genotype, RNA-Seq and clinical phenotypes. Dietary intake was collected by food frequency questionnaire. The associations between dietary intake and adipose fatty acids were tested with linear mixed models, adjusting for age, smoking, physical activity, index of multiple deprivation, energy intake, and relatedness. Genome-wide association studies (GWAS) were performed adjusting for age.The association between dietary scores, food intake, nutrient intake and fatty acid levels in adipose tissue were examined. Most dietary scores were positively correlated with polyunsaturated fatty acid (PUFA) but negatively correlated with trans-unsaturated fatty acid (TFA) (PFDR &lt; 0.05). We highlighted the positive association between polyunsaturated margarine and fish intake and PUFA levels, as well as butter and cream intake and saturated fatty acid (SFA) levels (PFDR &lt; 0.05). Negative associations between fresh red meat including lamb and beef, butter, and cream intake and PUFA levels in adipose tissue were observed (PFDR &lt; 0.05). Regarding nutrient intake, PUFA, SFA, TFA, cholesterol, vitamin D, and vitamin E were correlated with fatty acid levels in adipose tissue (PFDR &lt; 0.05). To reveal local genetic regulation of fatty acids in adipose tissue, we performed GWAS and identified 10 fatty acid-associated genetic loci across 13 fatty acids (i.e. palmitic acid/palmitoleic acid – at the SCD locus, dihomo-γ-linolenic acid/arachidonic acid at the FADS1 locus, P &lt; 5 × 10-8). The integration of adipose gene expression data revealed the mediation effects of SCD and FADS1 expressions in the associations between FADS1 SNP and the conversion of unsaturated fatty acids. We took forward two GWAS lead SNPs (SCD SNP and FADS1 SNP) to test the SNP-by-diet interaction on adipose fatty acids. Milk intake showed SNP-by-diet interaction with FADS1 SNP for linoleic acid and docosapentaenoic acid levels (P &lt; 0.001). The interactions between roasted potatoes/chips and FADS1SNP were significant for dihomo-γ-linolenic acid/linoleic acid (P &lt; 0.001).Adipose fatty acid levels were regulated by both genetic variants and dietary intake. We found suggestive evidence for the interaction of genetic variant and diet.