Levels Of Microbial Colonization Research Articles

Efficacy comparative characteristics of antiseptic DECASAN Yuria-Pharm and CUTASEPT G in the treatment of purulent inflammatory diseases of various localization

Background: High mortality because of various infectious complications (immediate cause in 42.5 % of cases), the ever-increasing frequency of purulent inflammatory diseases, as well as the growth of microbial resistance to anti-bacterial drugs, force to seek a solution to the problem among local antiseptics. Aim: to compare the effectiveness of antiseptics DECASAN Yuria-Pharm and CUTASEPT G in the treatment of puru-lent inflammatory diseases of different localization.Materials and methods: The study involved 30 patients with purulent inflammatory diseases of various localiza-tions. Patients were divided into two groups, according to the chosen tactics of local antiseptic therapy, in patients of the first study group (n = 15; mean age – 49.73 ± 6.35 years) antiseptic treatment was performed with a drug based on 0.02 % decamethoxine (Decasan). In the second group (n = 15; mean age – 51.4 ± 5.14 years) treatment was performed with a disinfectant (skin antiseptic) “Cutasept G”. Collection of material for microbiological examination (bacteriological culture) was performed before treatment, 3 and 5 days after treatment. Probability analysis was performed according to Student’s t-test. A statistically significant difference between the indicators was considered when the probability of validity of the null hypothesis was less than 5 % (p <0.05).Results: Analyzing the results of bacteriological examination of both groups, in the first, where Decasan was used and in the second, with the use of Cutasept G, it was found that at the beginning of treatment there was a pronounced microbial colonization of wounds, not significantly different among patients in both groups (p <0.05). Microbio-logical observation of the course of purulent-inflammatory process in the dynamics showed a significantly slowed down process of microbial purification in the second group (Cutasept G), the condition for 5 days was lg (8.8 ± 0.4) CFU/ml. At that time for patients of the first comparison group the number of microorganisms in 1 ml of wound contents, expressed in lg, was (4.8 ± 0.4) CFU/ml, which does not exceed the critical level. The obtained values were significantly higher than with Cutasept G (p <0.05), which was twice higher than the level of microbial colonization compared to the treatment of wounds with 0.02 % decamethoxine solution (p <0.001).Conclusions: The use of antiseptic 0.02 % decamethoxine for 5 days provides a reduction in the number of oppor-tunistic pathogens lg (4.8 ± 0.4) CFU/ml, which is 1.9 times lower (p <0.05) compared with the use of Cutasept G, creating conditions for the settlement of normal microbiotic skin (Corynebacterium spp., S. epidermidis). Antiseptic Dekasan is characterized by good healing properties due to its high antimicrobial activity, isotonicity, which makes it possible to use it successfully in patients with purulent inflammatory diseases of various localizations. In addition, the use of Dekasan promotes rapid cleansing of wounds from purulent-necrotic contents and formation of granula-tion tissue in them.

Local experience of real-time PCR implementation into microbiological monitoring programs

Objective. To study level and structure of microbial colonization in cancer patients before admission to a surgery department using molecular methods. Materials and Methods. The presence of DNA of methicillin-resistant Staphylococcus aureus, methicillinresistant coagulase-negative staphylococci, Candida albicans/C. glabrata/C. krusei, Acinetobacter baumannii in the clinical specimens and the presence of antimicrobial resistance genes in cultures were detected by multiplex real-time PCR. A total of 741 clinical samples and 313 cultures were studied. Results. The high level of microbial colonization, including microorganisms with antibiotic resistance genes, was found in cancer patients. Methicillin-resistant staphylococci, A. baumannii and Candida spp. were detected in 56.6%, 20% and 24.8% of cancer patients, respectively. Of patients infected with A. baumannii, 35.9% had OXA-carbapenemase genes. A. baumannii producing blaOXA40 was the most common cause of infectious complications. Conclusions. Molecular methods can be used effectively not only for the diagnosis of infectious diseases, but also in microbiological monitoring programs.

Bacterial and fungal colonization and decomposition of submerged plant litter: consequences for biogenic silica dissolution.

We studied bacterial and fungal colonization of submerged plant litter, using a known Si-accumulator (Equisetum arvense), in experimental microcosms during one month. We specifically addressed the microbial decomposer role concerning biogenic silica (bSiO2) dissolution from the degrading litter. To vary the rates and level of microbial colonization, the litter was combined with a range of mineral nitrogen (N) and phosphorous (P) supplements. Overall microbial growth on plant litter increased with higher levels of N and P. There was a tendency for higher relative bacterial than fungal stimulation with higher nutrient levels. Differences in microbial colonization of litter between treatments allowed us to test how Si remineralization from plants was influenced by microbial litter decomposition. Contrary to previous results and expectations, we observed a general reduction in Si release from plant litter colonized by a microbial community, compared with sterile control treatments. This suggested that microbial growth resulted in a reduction of dissolved Si concentrations, and we discuss candidate mechanisms to explain this outcome. Hence, our results imply that the microbial role in plant litter associated Si turnover is different from that commonly assumed based on bSiO2 dissolution studies in aquatic ecosystems.

Feeding preference of the South American endemic anomuran Aegla platensis (Decapoda, Anomura, Aeglidae)

In order to determine the feeding preference of Aegla platensis in streams and the importance of microorganisms in its detritivore diet, we carried out two experiments designed to evaluate the food preferences of A. platensis (1) among leaves with different levels of microbial colonization and (2) among insect larvae (Chironomidae, Simuliidae, Hydropsychidae) and microbially conditioned leaves. A. platensis preferred animal over plant food items; when only leaves were offered, this aeglid preferred the leaves with higher levels of microorganism conditioning.