The present study was performed under natural environment to assess levels of different heavy metals in soil and Abelmoschus esculentus plants along with soil microbial population irrigated with five rates of distillery effluent (DE) viz. 10, 25, 50, 75 and 100% concentration in comparison with control (Bore well water). Results revealed that among various concentrations of DE, irrigation with 100% DE significantly (P < 0.001) increased Zn (+63.46%), Cu (+292.37%), Zn (+3763.63%), Cd (+264.29%), Ni (+48.39%) and Cr (+815.74%), while decreased total bacteria (-45.23%), fungi (-17.77%) and actinomycetes (-42.57%) in the soil. Enrichment factor of various heavy metals for soil was in the order Ni > Cr > Cd > Zn > Cu, and for A. esculentus plants, it was in the order Ni > Cr > Cu > Cd > Zn after irrigation with distillery effluent. The enrichment factor value was found maximum for Ni in comparison to other metals at 100% DE concentration as compared with BWW. However, the values of these metals were below the recommended permissible limit.