The purpose of this study is to explore the factors of University Teachers’ motivation and the differences among the factors under different background variables. Based on a great deal of literatures, this paper classifies the incentive content of teachers in universities and colleges into two aspects: internal incentive and external incentive. Through constructing the incentive structure equation model, this paper analyzes and summarizes the influence factors of the incentive of teachers in universities and colleges from two aspects: internal incentive and external incentive, and finds that external incentive is divided into salary and welfare, organizational environment, career development, and internal incentive is divided into work achievement, individual value, as well as innovation incentive. On this basis, we find that there are significant differences in incentive level based on the characteristics of demographics. Among them, there are significant differences in the factors, including marital status and external incentive. There are significant differences in salary and welfare, organizational environment, work achievement and individual value among different ages. There are significant differences in career development of whether undertaking part-time administrative posts. There are significant differences in salary and welfare, organizational environment and career development among different teaching ages. There are significant differences in organizational environment and career development between different titles. There are significant differences in salary and welfare, organizational environment and incentive between different educational backgrounds, and there are significant differences in innovation incentive between different school types.