Levels Of Hematological Parameters Research Articles

Evaluation of acute and subacute toxicity of the extract of allium cepa peels in Wistar rats

Allium cepa is a popular recipe used traditionally to treat diabetes, cardiovascular diseases, cancer, wounds and infections. The acute and sub-acute oral toxicity profile of the aqueous extract of Allium cepa peels were evaluated in wistar albino rats in order to determine its safety for use in traditional medicine. The median lethal dose (LD 50) body weights and food intake were determined. The acute oral toxicity of the peel extract was carried out in accordance with OECD guideline 423. In addition to this, the haematological parameters and biochemical parameters were also evaluated. The median lethal dose (LD 50) of the extract was greater than 2000 mg/kg when given orally in the rats. There were no significant changes in the level of haematological parameters and biochemical parameters. The results from this study indicates that Allium cepa peels are relatively safe, hence supporting its use in traditional medicine.

Comparison of Hematological Profile of Athletes in Various Phases of Menstrual Cycle

The purpose of the study was to compare the hematological parameters during the different phases of the menstrual cycle viz Menstrual, Follicular, Ovulation, and Luteal Phase. Thirty athletes were selected as subjects for the study who were involved in speed-endurance dominated sports. Blood samples were taken in each phase and analyzed for the following hematological variables - Red blood cells (RBCs), White blood cells (WBCs), Hemoglobin (Hb), and platelets. The results of the study showed variations in hematological parameters throughout the menstrual cycle. RBC and Hb levels significantly increased during the follicular phase (FP) but remain unchanged in the ovulation and luteal phase. WBC levels also increased during the FP, were almost similar in ovulation phase, and slightly decreased in the luteal phase. Platelet count followed a similar pattern as WBC. Platelet count increased in the FP but decreased in the luteal phase. Menstrual phase is characterized by hemorrhage which results in blood loss and decreased levels of hematological parameters. FP is seen as compensatory phase in which the hematological variables significantly increase. WBC and Platelet count decrease in the luteal phase in attributed to the decrease in the estrogen levels. The study concluded that variations in hematological parameters is observed during the different phases of the menstrual cycle.

Comparison of the Levels of Hematological Parameters at Rest and after Maximum Exercise between Physically Active People with Spinal Cord Injury and Able-Bodied People.

The aim of the study was to reveal the difference in the hematological reaction to the applied exercise-induced workload between the able-bodied and physically active people with cervical spinal cord injury. The study covered 11 males with spinal cord injury and 11 able-bodied persons. An incremental stress test was carried out until the maximum individual workloads were achieved. The peak oxygen uptake was measured with the use of the ergospirometric method. Venous blood test results at rest and after finishing the maximal exercise showed hemoglobin (Hb) concentration, hematocrit (HCT) value, erythrocytes (RBC), leukocytes (WBC) and platelets (PLT) counts as well as the relative percentage of granulocytes (GRA), lymphocytes (LYM), and monocytes (MON). RBC, HCT as well as Hb and PLT among people with the injury were statistically lower (p < 0.001) large effect size, than in the control group. Statistically significant difference between the test and control group, subjected to the maximal exercise stress test, was observed in the exercise induced change of the PLT [p < 0.001, (ES: 2.631)] WBC [p < 0.05, (ES: 1.429)] and the percentage of LYM and GRA [p < 0.05, (ES: 1.447) for LYM and (ES: 1.332) for GRA] between both groups, subjected to the maximal cardiac stress test on the manual cycloergometer. The analysis of the obtained results indicates that people with spinal cord injury are much more vulnerable to the occurrence of microcytic anemia compared to able-bodied people. The after-exercise percentage shift of selected subpopulations of leukocytes in both groups indicates a delayed post-exercise recovery among people with spinal cord injury.

Effects of ambient temperature during the nestling stage on a stress indicator in nestling pied flycatchers Ficedula hypoleuca

Long-term and short-term changes in ambient temperature can cause stress in birds, leading to changes in the level of hematological parameters. The H:L ratio (heterophil-to-lymphocyte ratio) is a hematological index that allows for the assessment of the stress induced by environmental changes, including weather conditions. In this paper, we examined the influence of temperatures and the sum of precipitation on the health of nestling pied flycatchers (Ficedula hypoleuca) by using the H:L ratio reflecting the body’s response to stress. All examined temperature indicators influenced the H:L ratio, yet the average value of daily minimum temperature during the first 12 days of nestling life had the strongest influence, maximum temperature had the weakest effect, while precipitation had no significant influence. Our research indicates that even a small increase in temperature caused a stress reaction in nestling pied flycatchers, which was reflected by an increase in the H:L ratio. The increase in the stress index (H:L ratio) was probably a result of poor weather conditions (precipitation, low temperature), which prevented the adult birds from actively foraging and properly feeding the nestlings.

Prognosis of iron deficiency anemia in pregnant women with different somatotypes

BACKGROUND: Anemia during pregnancy, undiagnosed and untreated promptly, is the cause of various obstetric complications: spontaneous miscarriages, premature birth, placental insufficiency, obstetric bleeding, ante- and intrapartum fetal death.&#x0D; AIM: The aim of this study was to evaluate the incidence of iron deficiency anemia in pregnant women with different somatotypes and to develop a prognostic model for the pathology onset.&#x0D; MATERIALS AND METHODS: We examined 390 pregnant women. Somatometry was performed according to the method of R.N. Dorokhov in terms of pregnancy not exceeding 9-10 weeks. Of the examined pregnant women, 110 were of the macrosomatotype, 173 of the meso- and 107 of the microsomatotype. In a clinical blood test, the levels of hemoglobin and red blood cells were determined using well-known methods. Blood iron levels were evaluated by the colorimetric method with ferrozine using a Parma Iron Reagents Kit (Parma Diagnostics Ltd., Russia). Serum hepcidin levels were determined spectrophotometrically using ELISA methods.&#x0D; RESULTS: Iron deficiency anemia was most commonly detected in pregnant women of the macro- and microsomatotype, when compared to those of the mesosomatotype (p 0.05). There was no severe anemia in the study groups. The levels of hematological parameters (serum iron and serum hepcidin) were significantly higher in the group of pregnant women with latent anemia, compared to the study group without signs of anemia (p 0.05). In the second trimester, iron deficiency anemia occurred in the group of patients with latent anemia. Using multiple regression analysis, a formula was obtained for predicting the onset of iron deficiency anemia in pregnant women of different somatotypes.&#x0D; CONCLUSIONS: Hematological parameters (serum iron and serum hepcidin) should be attributed to markers of iron deficiency anemia and timely predict the onset of pathology. The mathematical formula obtained allows predicting with high accuracy the onset of iron deficiency anemia in pregnant women, taking into account the somatotype in the first trimester of pregnancy, and timely preventing the onset of pathology.

Effect of Flattening Filter and Flattening Filter Free beams on radiotherapy-induced peripheral blood cell damage

The purpose of this study is to investigate whether Flattening Filter (FF) and Flattening Filter Free (FFF) beams cause any difference on rat peripheral blood levels in a single dose (SD) and fractioned dose (FD) plans.Forty Sprague Dawley rats were randomly divided into five groups. No action was taken on the subjects in the control group (Group 1). FF-SD (Group 2) and FF-FD (Group 4) subjects received radiotherapy at 400 MU/min in SD 16 Gy and 5-day consecutive FD 32 Gy, respectively. FFF-SD (Group 3) and FFF-FD (Group 5) subjects received radiotherapy at 1400 MU/min in SD 16 Gy and 5-day consecutive FD 32 Gy, respectively. Blood samples were collected 24 h after radiotherapy. The levels of white blood cells (WBC), granulocytes (GRAN%), red blood cells (RBCs), lymphocytes (LYM%), hemoglobin (HGB) and platelets (PLT) were analyzed.In SD and FD plans, the levels of hematological parameters except for PLT level due to FF and FFF beams decreased statistically significant when compared with the control group (p < 0.05), but it was observed that the values of these parameters decreased less in the FD plans. In addition, when the effects of FF and FFF beams on peripheral blood cells were compared, a statistically significant difference was observed (p < 0.05).It was shown that FF and FFF beams significantly decrease hematological parameter levels in SD and FD plans. In addition, it was concluded that FFF beams were more dominant when we compared the effects of FF and FFF beams on peripheral blood cells.

Changes in Hematological Parameters and Liver Enzymes During Laparoscopic Cholecystectomy.

BackgroundChanges in hematological parameters, such as neutrophils, leukocytes, neutrophil-lymphocyte ratio, platelet lymphocyte ratio, and mean platelet volume, have been observed during laparoscopic surgeries.ObjectivesThe objectives of this research were to assess the changes in hematological parameters and liver enzymes during laparoscopic cholecystectomy (LC).MethodsThis prospective observational study included patients undergoing laparoscopic cholecystectomy for symptomatic cholelithiasis. Patients with comorbidities, including hepatitis, diabetes, and where laparoscopic cholecystectomy was converted to open cholecystectomy, were excluded. Preoperative and postoperative baseline hematological parameters and liver function tests (LFTs) were recorded. Characteristics like age, gender, body mass index (BMI), indication for surgery, duration of surgery, the pressure of pneumoperitoneum, and the duration of hospital stay were noted. A paired sample t-test was applied to assess the difference between the mean pre and postoperative values of different hematological parameters.ResultsIt was observed that hemoglobin (Hb), hematocrit (Hct), platelets, and alkaline phosphatase (ALP) decreased postoperatively. However, mean corpuscular volume (MCV), mean platelet volume (MPV), leukocytes, and alanine transaminase (ALT) increased postoperatively. The difference in mean Hb, MCV, Hct, leukocytes, MPV, and ALT was statistically significant (p<0.05).ConclusionThere were significant changes in the levels of hematological parameters and liver enzymes during LC.

Adverse effects of iron deficiency anemia on pregnancy outcome and offspring development and intervention of three iron supplements

Iron deficiency anemia (IDA) is a common micronutrient deficiency among pregnant women with severe consequences including impaired immuno-inflammatory system, premature birth, fetal death etc. The present study aimed to investigate the effects of three iron supplements on IDA female rats and their offspring. The IDA female rat model was established with low iron diet and the rats were then mated. After pregnancy, rats were fed diets containing different iron supplements (iron polysaccharide complex, iron protein succinylate and ferrous sulfate) until their offspring were 42 days old. Pregnancy outcomes, haematological, iron metabolism, physical and neurological development indexes were determined. The results showed that all three iron supplements improved the levels of hematological parameters of both mother and offspring rats. After iron supplementation, serum iron, transferrin saturation and serum ferritin levels were increased compared with the IDA group. The level of ferritin light chain in the liver and spleen of both mother and offspring rats in iron supplemented groups was significantly higher than that of the IDA group. The average number of born alive per litter in the iron treatment groups was significantly higher than that in the IDA group. Iron supplements also improved the physical growth and neurobehavioral development of offspring rats. It was also found that iron supplementation improved the expression of ferritin light chain and the synaptic growth associated proteins in the brain and hippocampus. No significant difference was found in the efficacy of three iron supplements. These results suggest that pregnant and postpartum IDA affects pregnancy outcomes, offspring physical development and causes neural impairment. Sufficient iron supplementation can significantly improve IDA and its adverse effects on both mother and offspring.

Profiling of Haematological Indices in Relation to Body Score and Altitude Among Norwegian Goats in Morogoro, Tanzania

Blood samples were collected from 180 apparently healthy adult Norwegian goats each 90 Norwegian goats, from high altitude and low altitude in Morogoro urban and peri-urban to assess the influence of altitude on their hematological indices. The (RBC) Red blood cells, PCV (packed cell volume), WBC (White blood cells) and HB (Hemoglobin) concentration were determined. Analysis of hematological indices was done using Automated Hematological Analyzer (Msn4s). Data on age, weight, body score, health and nutritional status were recorded for each animal. Epi-info version7.1 was used to analyze data for means, standard deviation and percentages. T-test and Chi-square were used to compare similarities between the groups. Results were compared to normal ranges of the parameters. Most parameters were within normal ranges. However significant increase in white blood cell count and decrease in hemoglobin concentration was observed from blood collected from groups of Norwegian goats located at low altitudes. Whereas the value of RBC (Red blood cells), PCV (packed cell volume) and HB (Hemoglobin) concentration in blood samples from high altitude were significantly high compared to those found at low altitude. These results revealed that, the level of hematological parameters were significantly affected by nature of areas animal located, that is altitude, although other confounding factors such as age, diseases, stress, sex, body weight and climatic condition may result to variations on the blood indices.

Pharmacological and Biochemical Evaluation of Anti-arthritic Activity of Punica Granatum Extracts in FCA Induced Arthritis in Wistar Rats

The present studies were executed on Freund's complete adjuvant (0.1 ml) induced arthritic Wistar rats to explore the folkloric use of the seeds. This study describes the effect of Punica granatum Linn's Ethanolic (PGSE) and Chloroform (PGSC) extract within the FCA-induced arthritis rat paw oedema, vagaries in behaviour, haematological and alterations in biochemical parameters in the developed and progression of arthritis phases. There was a significant rise in the paw swelling (volume) of rats and reduction in (BW) body weight, with FCA-induced arthritic rats. In contrast, PGSE and PGSC with the dose of 200mg/kg and 400mg/kg and Diclofenac (20mg/kg) treated group showed a substantial decrease in paw volume and the normal improvement in body weight to the positive control group. The altered level of haematological parameters, including, Hb, RBC, WBC, and ESR, in arthritic rats, have been substantially regained to normal by PGSE and PGSC treatment at the dosage of 200 mg/kg/p.o and 400 mg/kg/p.o in both the developed and progression of arthritis phases. In this study, Anti-inflammatory action of the PGSE and PGSC with carrageenan-induced paw oedema has also been investigated. Thus, percentage (%) inhibition of PGSE and PGSC were found to be 91.8 % and 86.71, respectively, concerning Diclofenac sodium (93.14%), this gave the evidence of dose-dependent action potential of Punica granatum as anti-inflammatory activity.

The Prevalence and Risk Factors of Iron Deficiency Anemia in Thai Infants by Complete Blood Count at 9-Month-Old

Background: Infants are the most at risk from iron deficiency (ID) and iron deficiency anemia (IDA), but the prevalence of the latter in this group is unclear. Estimation as to the prevalence of IDA using Hb of less than 11 g/dL may have been exaggerated due to thalassemia and hemoglobinopathies. On the other hand, iron studies are costly and not routinely available in low resource settings. In the present research, the authors estimated the prevalence of IDA in 9-month-old infants at the authors’ well-baby clinic by improving the post-iron-treatment criteria of Hb and defined the risk factors of IDA in infants. Objective: To estimate the prevalence of IDA in 9-month-old infants at the well-baby clinic and to define the risk factors of IDA. Materials and Methods: A retrospective cohort study of 391 children who had complete blood count (CBC) screening at 9-month-old at the well-baby clinic. Anemia was defined according to the WHO criteria (hemoglobin of 11 g/dL or less for children aged six months to five years). IDA was defined as an increase of at least Hb 1 g/dL after receiving iron treatment for one to two months. Mean levels of hematological parameters were compared between groups using independent samples t-test. The characteristics with potential risk factors for IDA were compared with iron non-responder and normal group. Multivariable adjusted odd ratios and 95% confidence intervals (CIs) of IDA were calculated using multinomial logistic regression with control for risk factors. Results: One hundred fourteen children (29.1%) were anemic. There was no difference between anemic and non-anemic children in baseline characteristics. Seventy children in the anemic group were diagnosed with IDA. The prevalence of IDA in the present study was 17.9%. The IDA group showed significant improvement in Hb, Hct, MCV, MCH after receiving iron treatment, but there were no significant changes in the iron non-responder group. The risk factor for IDA was exclusive breastfeeding for at least six months (odds ratio 3.14, 95% CI 1.67 to 5.90, p&lt;0.001). Conclusion: The prevalence of IDA and anemia in the present study was 17.9% and 29.1%, respectively. The risk factor for IDA was exclusive breastfeeding for at least six months. Keywords: Anemia, Iron deficiency anemia, Prevalence of anemia in infants, Complete blood count screening, Thai infants

Cholinesterase inhibition and its association with hematological, biochemical and oxidative stress markers in chronic pesticide exposed agriculture workers.

The present study investigated the pesticide induced adverse health effects, hematological and biochemical alterations among agriculture workers. A cross sectional study of 51 agriculture workers and 54 unexposed subjects was carried out to evaluate hematological and biochemical alterations in blood. Pesticide exposed individuals were reported adverse clinical outcomes, including tingling, muscle pain, headache, skin disease, etc. A significant alterations in the level of hematological parameters, liver and renal dysfunctions markers and lipid profile suggested hematological, hepatic and renal dysfunctions. A significant decrease in the activity of acetylcholinesterase, reduced glutathione, superoxide dismutase, catalase and increased level of lipid peroxidation was also observed in these agriculture workers. Correlation coefficient analysis showed a positive correlation of chronic exposure with most of the hematological and biochemical parameters. The results demonstrate that the chronic exposure of pesticides cause reduction in the acetylcholinesterase activity and enhanced the risk of adverse clinical outcomes in agriculture workers.

Variation of Heterophil-to-Lymphocyte Ratio in the Great Tit Parus major — a Review

Permanent changes in the surrounding environment cause long-term stress in birds, which, when lasting days or weeks, affects the activity of the immune system and increases susceptibility to diseases, leading to changes in the levels of haematological parameters. The heterophil-to-lymphocyte ratio (H:L-ratio) is generally considered an independent and robust indicator of stress level in birds. This parameter allows in a simple way to evaluate activity of the immune system and individual health state of adult and nestling birds. It also enables assessing a body response to short- and long-term stress induced by, among others, the surrounding environment, social stress, blood parasites or a greater energy expenditure of females during breeding. Under conditions of field work the determination of the H:L-ratio is not difficult because what is only needed to conduct a blood smear test is a drop of blood that can be easily obtained even from birds of a small body mass. Moreover, an increase in the H:L-ratio is observed after about an hour from the moment of catching a bird contrary to other measurements like the determination of a baseline level of corticosterone.In this article available literature that discusses the impact of various factors on the H:L-ratio in the Great Tit as a species of ‘fast-paced’ life is reviewed. In adult and nestling birds the H:L-ratio is influenced by various factors — ecological and ecophysiological ones. In some cases the same factor, e.g. brood size manipulation or a type of habitat, can significantly influence the level of the discussed stress indicator as well as it may not show any impact at all. While interpreting the H:L-ratio one must take into account an impact of various ecological and ecophysiological factors on health state, such as habitat, phase of the annual cycle, differences between brood attempts, sex, age as well as on relations with other indicators of condition e.g. body mass or total blood haemoglobin concentration.

Iron Metabolism and Oxidative Status in Patients with Hb H Disease

To evaluate the iron metabolism and oxidative status in patients with Hb H disease, we investigated 43 patients with Hb H disease, including eight deletional Hb H disease patients and 35 nondeletional Hb H disease patients and 20 healthy controls. The levels of hematological parameters, serum ferritin, hepcidin, superoxide dismutase (SOD), malondialdehyde (MDA) and total antioxidant capacity (TAC), were examined. We found higher serum ferritin levels and lower hepcidin, MDA and TAC levels in Hb H disease patients than in controls. The hepcidin level in Hb H disease patients was positively correlated with MDA and TAC levels but not with serum ferritin and SOD levels. The patients with nondeletional Hb H disease showed higher serum ferritin and Hb H concentrations than those patients with deletional Hb H disease. However, no statistically significant differences in SOD, MDA and TAC levels were found in patients with deletional and nondeletional Hb H disease. Oxidative stress and antioxidant defense were related to hepcidin levels. Our study indicated that hepcidin might be an important parameter for monitoring the iron metabolism and oxidative status of Hb H disease patients.

Evaluation of Efficacy diet supplemented with some local formulas on body weight and haematological parameters of rats

Some local traditional formulas have been commonly used based on the information of their popularity in local markets for gaining weight. Hence, the aim of this study was conducted to evaluate the effect of some local prepared formulas (Mefataka, Kharaz El-kortas El-Abiad) on body weight, hematological parameters and their efficacy in case of long-term consumption (8 weeks), using experimental animals. Forty two adult male albino rats were divided into seven groups (6 rats each), group (1) was fed on basal diet and kept as a control group, groups (2 and 3) were fed on basal diet supplemented with formula (1) at the level of 10% and 15 % of the rats diet respectively. Groups (4 and 5) were fed on basal diet supplemented with formula (2) kharaz El-Bakar, at the level of 10% and 15 % of the rats diet respectively. Groups (6 and 7) were fed on basal diet supplemented with formula (3) Tahwijat El-kortas at the level of 10% and 15 % respectively. The chemical composition of studied formulae Mefataka, Kharaz El-Bakar and El-kortas El-Abiad provided caloric value of 337.01, 346.44 and 395.7 K.cal/100g dried sample respectively. The results indicated that the body weight gain percent and feed efficiency ratio were increased significantly (P<0.05) in rats fed on their formulae, compared to the control group. Also, significant increase (P<0.05) in the levels of hematological parameters and leptin hormone were observed, however, there were no significant changes in liver and kidney functions, compared to the control group. Conclusion: The tested formulae increased the weight of rats without any harm on body systems. A trial of using these formulae on slim human seeking an increase in body weight is recommended.

Antidiabetic Activity of Triticum aestivum Seed–Based Diet on Alloxan-Induced Diabetic Rats

The antidiabetic activity of Triticum aestivum seed–based diet on alloxan-induced diabetic rats was investigated. Forty-eight male and female albino rats of four groups were used for this study. Rats were sacrificed on day 28 and organs of interest were excised. Triticum aestivum seed–based diet significantly (p < .05) reversed the levels of fasting blood glucose, albumin, globulin, bilirubin, urea, creatinine, Na+, and K+. In addition, diabetic rats fed Triticum aestivum seed–based diet had significantly (p < .05) increased insulin and glycogen concentrations, activities of hexokinase, catalase, superoxide dismutase, and glutathione peroxidase and the levels of hematological parameters studied. Diabetic rats fed on Triticum aestivum seed had significantly (p < .05) reduced activities of glucose-6-phosphatase and fructose 1,6-diphosphatase and concentration of MDA and reversed activities of AST and GGT; ALP and regeneration of liver, kidney, and pancreas tissues compared favorably with the control group from histological examination results. Consumption of this diet may be useful for diabetes mellitus patients in ameliorating diabetes mellitus and its complications

Haematology of N’Dama and West African Short Horn cattle herds under natural Trypanosoma vivax challenge in Ghana

Background: Animal trypanosomosis is a major cause of economic loss in livestock production in Africa. A suggested control measure is to use breeds with traits of trypanotolerance. The study examines the effect of natural Trypanosoma vivax challenge on haematological parameters in two trypanotolerant cattle [N’Dama and West African Short Horn (WASH)] herds. Methods: T. vivax-specific primers were used to diagnose T. vivax infection in an N’Dama herd at Cape Coast in southern Ghana and a WASH herd at Chegbani in northern Ghana from May to July 2011 in a cross-sectional study. Levels of haematological parameters comprising packed cell volume (PCV), haemoglobin (Hb) concentration and total red blood cell (RBC) and white blood cell (WBC) counts; differential WBC counts (neutrophils, lymphocytes, eosinophils, monocytes and basophils); and RBC indices of mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH) and mean corpuscular haemoglobin concentration (MCHC) were determined in blood samples and then compared between infected and uninfected cattle. Results: We found that haematological indices for infected and uninfected animals in both breeds were within the normal range. However, the mean PCV values for T. vivax-infected WASH and N’Dama were lower in infected compared to uninfected animals. The difference was significant (p&lt; 0.05) in N’Dama but not in WASH. The RBC indices were higher in infected N’Dama compared to infected WASH with a significant difference in total RBC (p &lt; 0.05). Conclusion: We conclude from our findings that despite the presence of infection by T. vivax, N’Dama and WASH cattle maintained their haematological parameters within acceptable normal ranges, and this underscores the need for routine diagnosis and treatment so that such trypanotolerant cattle do not serve as potential reservoirs of trypanosome parasites.

Dietary Application of the Probiotic Lactobacillus plantarum 426951 Enhances Immune Status and Growth of Rainbow Trout (Oncorhynchus mykiss) Vaccinated Against Yersinia ruckeri.

This study was aimed to assess the effect of oral application of Lactobacillus plantarum (2×107CFUg-1 feed) as a probiotic on growth performance and immune status of vaccinated rainbow trout (29.5 ± 2g) to yersiniosis at 16 ± 2°C for 72days. Fish were randomly allocated into 12 fiber glass tanks (4100L) at a density of 80 fish per tank (240 fish per treatment). The results revealed that the activity of lysozyme and alkaline phosphatase was significantly higher in immunized fish fed with diet supplemented with probiotic (vaccine +probiotic) than that in the immunized group fed with basal diet (vaccine group) while no significant differences in levels of hematological parameters, complements, total IgM, proteins, and the intestine lactic acid bacteria (LAB) were detected. Also, significantly a better growth performance in terms of feed conversion ratio, weight gain, and thermal growth coefficient was seen in the vaccine + probiotic group than that in the vaccine group. These results indicate that feeding probiotic after vaccination can enhance the efficacy of immersion vaccination to Yersinia ruckeri.

The value of neutrophil to lymphocyte ratio and platelet to lymphocyte ratio in salivary gland tumors.

e17592 Background: Salivary gland tumors are rare, comprising only 6 to 8 percent of head and neck neoplasms. Most of them are benign and commonly occur in the parotid gland. In the last decade elevated hematological inflammatory parameters like neutrophil to lymphocyte ratio (NLR) and platelet to lymphocyte ratio (PLR) were identified as predictive and prognostic factors in various malignancies. The aim of this study was to evaluate the differences of preoperative of NLR and PLR levels in benign and malignant causes of salivary gland tumors. Methods: This retrospective study was made of a total of 133 patients with salivary gland tumors (age range: 17-89 years; 68 male and 65 female) who were treated between January 2006 and May 2016. Preoperative counts and levels of hematological parameters were obtained from the recorded computerized database in benign and malignant tumors. Results: NLR and PLR levels were significantly higher in the malignant salivary gland tumor group than the benign group( NLR: 0.66±1.3 vs 0.59±0.31 p=0.001, PLR: 160.3±85.7 vs 124.6±44.1 p=0.014). Conclusions: The results of the current study revealed the potential predicitve role of NLR and PLR in patients with salivary gland tumors. These cheap and easily available blood count parameters could be useful biomarkers in the future; but further investigations are needed to confirm our findings. [Table: see text]

Homocysteine Metabolism and Hematological Parameters in Early Stage of Phenytoin Treated Epileptic Children.

Long-term antiepileptic drug (AED) therapy has been associated with metabolic consequences that lead to an increase in risk of atherosclerosis in patients with epilepsy. Earlier published studies showed conflicting results about the levels of hematological parameters, serum homocysteine, folate, and vitamin B12, in epileptics treated with phenytoin monotherapy. Therefore, we evaluated homocysteine metabolism and hematological parameters in early stage of phenytoin treated epileptic children. A total of 64 newly diagnosed epileptic children with mean age 10.09 ± 2.56 years were enrolled at the start of study. However, after 3 months follow up, the final total sample size was only 50 epileptic children. Fourteen children dropped out of study due to poor follow up. Serum homocysteine levels were measured by enzyme immunoassay method. Serum folate and vitamin B12 levels were estimated by Competitive Chemiluminescent Enzyme Immunoassay method. Hematological parameters were analysed by an automated hematology analyzer (Cell counter), Sysmex XT-1800i, using commercially available reagents. In our study the anthropometric and hematological parameters did not show any significant difference after phenytoin monotherapy as compared to before therapy in epileptic children. The serum homocysteine level in epileptic children was found to be significantly increased after phenytoin (PHT) monotherapy as compared to before therapy. Moreover, a highly significant decrease was observed in the serum folate and vitamin B12 levels after phenytoin monotherapy as compared to before therapy in epileptic children. Phenytoin monotherapy may cause a significant increase in the levels of serum homocysteine and a significant decrease in the serum folate and vitamin B12 levels in children with epilepsy, and the significant changes in above mentioned parameters occur early in the course of treatment. This could be responsible for a higher prevalence of cardiovascular incidents in epileptic children taking phenytoin monotherapy. Therefore, it may be useful to do early screening and treatment of increased serum homocysteine levels in epileptic children under phenytoin monotherapy to prevent atherosclerosis and its complications. Hematological parameters should also be strictly monitored regularly in individuals administered with PHT monotherapy. If there are persistent alterations, the administration of the drugs should be discontinued.