Levels Of Antioxidant Enzymes Research Articles

Impact of Nanoparticles on Oxidative Stress and Inflammatory Pathways in Human Prostate Cancer

Prostate cancer is one of the most prevalent cancers among men and is fueled by oxidative stress and inflammation that drive tumor progression and resistance to therapy. Production of reactive oxygen species (ROS) and pro-inflammatory cytokine such as tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) establish a pro-tumor microenvironment. Iron oxide nanoparticles are believed to be promising micro/nano therapy agents because NPs such as ferric oxide (Fe2O3) play a critical role in antioxidant and anti-inflammatory events. Herein, we examine influences of NPs on oxidative stress and inflammatory pathways in prostate cancer models. NP treatment was used for cells exposed to oxidative and inflammatory stressors. We measured a variety of key markers including ROS, MDA, GSH, and activities of antioxidant enzymes (catalase [CAT] and superoxide dismutase [SOD]). The levels of inflammatory cytokines were determined by widely used biochemical methods and spectrophotomedically. This resulted in significant reduction in ROS and MDA (oxidative damage markers) and significant elevation in the reduced GSH levels and these results were supported by the increased levels of antioxidant enzymes namely catalase (CAT) and superoxide dismutase (SOD) activity in NP-treated. Moreover, NPs also inhibited inflammation by downregulating the release of cytokines, such as TNF-α and IL-6. These effects suggest that NPs are capable of restoring oxidative homeostasis and suppressing inflammation, ultimately leading to a more tumor-hostile microenvironment. Abstract Nanoparticles that alleviate oxidative stress and overwrite inflammation pathways synergistically offer two critical blockade points in prostate cancer therapy to counter plasma phase-induced cellular malfunctions required for tumor progression. These results reinforce the prospect of NP as complements to cancer therapy. Optimizing NP formulations could improve their efficacy and safety for potential clinical applications in the future and provide new approaches for improving prostate cancer treatment. CuO NPs with Anti-cancer Effect on Prostate Cancer: In Vivo Study Oxidative Stress along with Inflammation as Potential Pathogenesis Prostate Cancer Responsive Ferric Oxide Nanoparticles Release ROS Assist in Treating Prostate Cancer.

Antioxidant Enzyme Responses in Cladoceran Moina macrocopa under Oxidative Stress

Oxygen is the cornerstone of the life of all living organisms, as it plays a pivotal role in regulating the bio-physiological functions of the organism that are necessary to sustain its existence. Oxidative stress/ hypoxia is a condition of inadequate oxygen that disrupts the harmony of an organism's life by impairing its growth, puts the organism's survival at risk, and destabilizes the ecosystem. Like all other organisms Moina, a small cladoceran is susceptible to the alteration in dissolved oxygen levels. Here we report that, in response to hypoxia, Moina adjusts their antioxidant enzyme activities as a mechanism of adaptation. Significant changes were observed in the levels of LDH, GST, and SOD antioxidant enzymes of Moina under hypoxia (p=0.001053, GST p=0.0010053, SOD p=0.0015). These results can contribute to wider research on environmental stress tolerance by aquatic organisms and will assist in the conservation of species like Moina. Moina, being an ecological indicator, this research will help in environmental monitoring using it as a model organism.

Impact of Feeding Frequency on Growth Performance and Antioxidant Capacity of Litopenaeus vannamei in Recirculating Aquaculture Systems

Feeding frequency is crucial for the growth and development of white shrimp (Litopenaeus vannamei) at various life stages. Although higher feeding frequencies can enhance growth, manual feeding methods significantly increase labor costs. Automatic feeding systems offer a cost-effective and efficient alternative, yet their application in intensive shrimp culture remains under-researched. This study evaluates different feeding frequencies for L. vannamei in intensive aquaculture tanks, focusing on growth performance, survivability, feed utilization, digestive and antioxidant capacities, and economic viability. Juvenile shrimp (3.85 ± 0.3 g) were cultured for 63 days with feeding frequencies of 6, 8, 10, and 12 times/day (A6, A8, A10, and A12 groups, respectively) using automatic feeders, with a control group fed manually 6 times/day (M6). Results indicated that automatic feeding significantly improved final body weight, specific growth rate, and feed conversion ratio compared to manual feeding. Among automatic feeding groups, A6 and A8 showed optimal performance, with a quadratic regression identifying 7.83 times/day as the optimal frequency. While digestive enzyme activity remained consistent across groups, A6 and A8 demonstrated significantly higher antioxidant enzyme levels (superoxide dismutase (SOD) and glutathione peroxidase (GPx)) and lower lipid peroxidation (MDA), suggesting superior digestive and antioxidant capacities. Pearson’s correlation confirmed a positive relationship between SOD and GPx. Economically, the A8 group achieved the highest profitability. Consequently, a feeding frequency of 6–8 times/day using automatic feeders is recommended as an optimal and feasible strategy for intensive white shrimp culture in this life stage.

Vitamin E improves the reproductive system of male rats exposed to busulfan chemotherapy.

Vitamin E is a well-known antioxidant and is frequently used as an adjunct treatment in cancer therapy. Busulfan is a commonly used drug for cancer treatment. In this study, twenty-eight male rats, ten weeks old and weighing between 250 and 300 grams, were divided into four groups. The first group served as the control and received daily intraperitoneal injections of Dimethyl sulfoxide (DMSO) for four weeks. The second group received a single dose of Busulfan at 40 mg/kg body weight via intraperitoneal injection. The third group received the same single dose of Busulfan along with daily intraperitoneal injections of Vitamin E at 100 mg/kg body weight for four weeks. The fourth group was given only Vitamin E at the same dosage for four weeks. At the end of the experiment, the animals were sacrificed, and blood samples were collected to test antioxidant enzyme levels (GSH, SOD, CAT) and analyze serum concentrations of reproductive hormones (FSH, LH, and testosterone). Additionally, sperm motility and viability were assessed after collecting epididymal spermatozoa. The findings revealed that Busulfan significantly increased serum levels of both FSH and LH while causing a notable decrease in testosterone levels. Furthermore, Busulfan treatment resulted in a significant reduction in sperm count, motility, and viability, along with a marked increase in sperm morphological abnormalities. In contrast, supplementation with Vitamin E alongside Busulfan improved hormone levels and enhanced sperm function. In conclusion, Busulfan has a toxic effect on sperm and directly impacts body weight and testicular weight. However, Vitamin E demonstrates beneficial therapeutic effects on testicular tissue and enhances sperm production in rats treated with Busulfan.

Impact of Lysine to Methionine Ratios on Antioxidant Capacity and Immune Function in the Rumen of Tibetan Sheep: An RNA-Seq Analysis.

With global protein prices on the rise, lowering protein levels in animal feed, together with balancing diet composition and reducing nitrogen emissions, can both reduce the environmental impact of agriculture and save on feed costs. However, the formulation of an ideal amino acid (AA) composition is crucial for better protein utilization by livestock. This study aimed to investigate the effects of different lysine to methionine ratios on the antioxidant capacity and immune function of the rumen in Tibetan sheep. Ninety male Tibetan sheep, weaned at 2 months of age, were randomly divided into three groups (1:1, 2:1 and 3:1 lysine ratios) and subjected to a 100-day feeding trial. RNA sequencing (RNA-seq) was utilized to analyse the impact of different AA ratios on gene expression in rumen tissue, whereas the levels of antioxidant enzymes (total antioxidant capacity [T-AOC], superoxide dismutase [SOD], glutathione peroxidase [GSH-Px] and catalase [CAT]) and immunoglobulins (immunoglobulin A [IgA], immunoglobulin G [IgG] and immunoglobulin M [IgM]) were evaluated. The results indicated that the 1:1 group significantly upregulated the expression of PTGS2, PLA2G12A and PLA2G4 genes, enhancing antioxidant enzyme activity, reducing free radical production and modulating systemic immune responses. COL16A1 and KCNK5 were highly expressed in the protein digestion and absorption pathway, maintaining the structural integrity and function of the rumen epithelium. BMP4 and TGFBR2 were significantly enriched in the cytokine-cytokine receptor interaction pathway and positively correlated with CAT and T-AOC. ITGA8 was upregulated in the 1:1 group, participating in the regulation of various cellular signalling pathways. ATP2B1 was enriched in the cyclic guanosine monophosphate (cGMP)- protein kinase G (PKG) signalling and mineral absorption pathways, primarily influencing oxidative stress and immune responses by regulating intracellular calcium ion concentration. This study demonstrates that a 1:1 lysine to methionine ratio is most beneficial for enhancing the antioxidant capacity and immune function of the rumen in Tibetan sheep.

Smilax china L. polyphenols inhibit LPS-induced macrophage M1 polarization to alleviate inflammation through NF-κB signaling pathway in vitro and in vivo.

As an important component of the cell wall of Gram-negative bacteria, lipopolysaccharide (LPS) is an important inducer of inflammation in humans. Smilax china L. is known for its diverse bioactive functions, particularly its anti-inflammatory effects. This study aimed to investigate the bioactive function of Smilax china L. polyphenols (SCLP) on LPS-induced inflammation. Inflammation in RAW264.7 macrophages and mice were induced using LPS. The cytotoxicity of SCLP was investigated by MTT assay. Inflammatory factors were detected by ELISA and RT-PCR. The expression of NF-κB pathway-related proteins was analyzed by Western Blotting. The results demonstrated that SCLP significantly reduced the levels of pro-inflammatory factors (TNF-α, IL-1β, and IL-6) and inhibited M1 polarization of macrophages in both RAW264.7 macrophages and mice (p<0.05). Western Blotting analysis revealed that the levels of NF-κB signaling pathway-associated proteins (p-p65, p-IKB, p-IKK) were significantly reduced (p<0.05). Notably, SCLP significantly downregulated the expression of pro-apoptotic proteins, while upregulating the expression of anti-apoptotic proteins in RAW264.7 macrophages (p<0.05). Additionally, the levels of antioxidant enzymes were enhanced in mice, suggesting a potential reduction in the inflammatory response. These findings indicated that SCLP might inhibit LPS-induced M1 polarization through the NF-κB signaling pathway, thereby reducing inflammation. Consequently, SCLP might serve as a promising bioactive substance for preventing inflammation-related injury.

Testosterone deficiency aggravates diet-induced non-alcoholic fatty liver disease by inducing hepatocyte ferroptosis via targeting BMAL1 in mice.

Testosterone deficiency is linked to an increased prevalence of non-alcoholic fatty liver disease (NAFLD), although the mechanisms underlying this association are not fully understood. Ferroptosis, a regulated cell death pathway driven by iron-dependent lipid peroxidation, has been suggested to play a role in NAFLD pathogenesis. Since testosterone deficiency is associated with lipid disorders and iron deposition, we hepothesize that ferroptosis may be involved in the pathogenesis of diet-induced NAFLD exacerbated by testosterone deficiency. Apolipoprotein E (APOE-/-) mice were subjected to sham surgery or bilateral castration and subsequently fed a high-fat diet for 16weeks. Liver gene expression was analyzed using RNA sequencing. Additional assessments included blood analysis, histological staining, measurement of iron and antioxidant enzyme levels, quantitative real-time PCR, Western blotting, and electron microscopy. The effects of testosterone on ferroptosis induced by free fatty acids (FFAs) and Erastin were further investigated in HepG2 cells in vitro. Testosterone deficiency resulted in increased hepatic lipid accumulation and macrovesicular steatosis in high-fat diet-fed APOE-/- mice, accompanied by hepatic inflammation, fibrosis, and elevated liver enzyme levels. Transcriptomic analysis revealed that testosterone deficiency affects ferroptosis and circadian rhythm-related signaling pathways. Castrated APOE-/- mice exhibited significantly higher hepatic iron deposition, lipid peroxidation, and expression of key ferroptosis-related proteins, along with decreased Brain and muscle ARNT-like gene 1 (BMAL1) protein expression. In vitro, testosterone treatment reduced lipid and iron accumulation and lipid peroxidation in HepG2 cells subjected to FFAs and Erastin. Moreover, BMAL1 knockdown negated the protective effects of testosterone against ferroptosis in hepatocytes. Our study demonstrated that testosterone deficiency exacerbates NAFLD induced by a high-fat diet by promoting hepatocyte ferroptosis through modulation of the circadian protein BMAL1.

In vivo, in vitro, and in silico approaches in the detailed study of di-butyl phthalate (DBP), a plasticizer-induced lung fibrosis via Nrf-2/Keap-1/HO-1 pathway and its regulation.

The alveolar epithelium is a crucial barrier against external threats, yet it becomes a key player in initiating pulmonary fibrosis when compromised. Despite its importance, the intricate relationship between, DBP exposure and alveolar epithelial cell injury ensuing pro-fibrotic effects remains poorly understood. Phthalates, ubiquitous in nature, pose a significant risk to lung health upon inhalation, acting as immune triggers that cause airway inflammation and epithelial damage. We aimed to investigate the impact of intranasal administration ofDi-butyl Phthalate (DBP) inhalation, and its probable effects on normal and asthmatic lungs. DBP was administered via intranasal route in normal and OVA-induced asthmatic mice. DBP exposure enhanced oxidative stress and inflammatory parameters, leading to exacerbated asthmatic response and oxidative lung damage. Enhanced accumulation of immune cells, bronchial thickening, and collagen deposition was noted in histopathological investigations of DBP-exposed lung sections. Curcumin, a plant-derived molecule, significantly mitigated DBP-exposed asthma exacerbations by suppressing NF-κB expression and enhancing NRF2 levels via the Nrf-2/Keap-1/HO-1 signaling pathway. FACS analysis revealed increased CD11b+ cells (32%) in asthmatic mice which were reduced in the curcumin pre-treatment group (10.5%). Enhanced epithelial to mesenchymal transition (EMT) was noted in mice lungs and A549 cells where E-cadherin expression was reduced as compared to Vimentin, and α-SMA. Apart from aggravated airway inflammation, DBP exposure damages healthy lungs also. MMP-9/TIMP-1 ratios and collagen-1 levels were restored which were enhanced after DBP exposure. Moreover, antioxidant enzyme levels such as NQO-1, HO-1, and Catalase were significantly enhanced (p<0.01) and comparable to dexamethasone, a conventional corticosteroid. Notably, both dexamethasone and curcumin treatments effectively regulated the stimulation and accumulation of Nrf-2 in the nucleus, promoting antioxidant production and offering potential therapeutic benefits in mitigating pulmonary fibrosis. OVA and DBP alone caused DNA damage in the lung cells where increasedpercentage of damaged DNA movement in thetail, tail length, tail moment, and olive tail moment indicated severe damage in theDBP and OVA combined exposure strategies. Dexamethasone and Curcumin treatments reduced theextent of the DNA damage indicating anti-inflammatory and ant-oxidative potentials. Moreover, in silico studies are supportive of therapeutic potential of Curcumin and Dexamethasone in DBP-induced lung inflammation and fibrosis.

Mining of antioxidant sesquiterpene lactones from the aerial parts of Saussurea involucrata with feature-based molecular network strategy.

Sesquiterpene lactones (SLs) are a class of natural products with diverse structural scaffoldings and biological activities, making them intriguing objects in the fields of pharmaceutical industry, drug development, and pharmacology. Herein, fifteen SLs, including eleven undescribed SLs compounds sauruintones A-K (1-8 and 13-15), were isolated and identified from the aerial parts of Saussurea involucrata. Their structures were characterized by using mass spectrometry, spectroscopic methods, computational calculations, and single crystal X-ray diffraction. The Feature-Based Molecular Network (FBMN) strategy was utilized for the annotation of SLs with their MS/MS fragmentation patterns. Most of the SLs exerted their protective effects against oxidative stress by enhancing the levels of antioxidant enzyme (CAT) and reducing the malondialdehyde (MDA) levels. Among them, compound 12 exhibited potent reactive oxygen species (ROS) inhibitory activities and enhanced the activities of CAT, superoxide dismutase (SOD), and glutathione peroxidase (GSH), and decreased the content of MDA at concentrations of 10-25 μM. Further RT-qPCR assays and western blot analysis revealed it reduced the level of ROS by activating the nuclear factor erythroid 2-related factor 2 (Nrf-2) signaling pathway.

Salicylalazine: A novel therapeutic agent targeting TLR4/NLRP3/GSDMD-mediated pyroptosis in rheumatoid arthritis.

Joint pain and functional impairment are hallmarks of arthritis, a painful inflammatory disease. Salicylalazine (SAZ), an anti-inflammatory compound, has demonstrated promise in modulating inflammation, thereby being selected in the current study to unveil its anti-arthritic potential. The aim behind this study was to assess the anti-arthritic properties of salicylalazine via evaluating its impact on paw volume, arthritic scores, oxidative stress indicators, and significant inflammatory mediators. The arthritic potential of SAZ was estimated first through the formaldehyde (FA) model to screen the most effective dose of SAZ, followed by the Complete Freund's adjuvant (CFA) model. Over a 28-day period, we monitored parameters such as paw edema, arthritic index, body weight, flexion pain, mobility, and stance score. Oxidative stress markers (GSH, CAT, SOD, MDA), serological markers (CRP, RF, anti-CCP), and gene expression of key inflammatory mediators (TLR4, MyD88, NFκB, NLRP3, ASC, IL-1β, IL-18, caspase-1, GSDMD) were assessed. SAZ treatment led to a substantial decrease in paw volume in both arthritis models, with the most pronounced effects observed on day 10 for the formaldehyde model and day 28 for the CFA model (p<0.001). Additionally, SAZ helped to restore the body's weight and considerably relieved the flexion pain, which led to improvements in both mobility and stance. Moreover, SAZ substantially raised the levels of antioxidant enzymes (GSH, CAT, SOD) and decreased MDA levels, suggesting a reduction in oxidative stress. Also with SAZ, there was a substantial (p<0.001) decrease in the expression of pyroptotic mediators. Serological markers of inflammation, including CRP, RF, and anti-CCP levels, were also restored by SAZ administration. In a nutshell, SAZ achieved significant anti-arthritic benefits, most likely via the modulation of the TLR4/NLRP3/GSDMD-mediated pyroptosis pathway, lowering oxidative stress, and improvement of clinical findings. Taking into consideration these findings, it seems that SAZ has the potential to be an effective treatment alternative for the management of arthritis.

A randomized, clinical trial of intravenous N-acetylcysteine as an antioxidant therapy in acute organophosphorus pesticide poisoning.

The incidence of acute organophosphate (OP) poisoning has steadily increased in developing countries. Many studies showed that oxidative stress could have a significant role in its mechanism. The current study aimed to evaluate the role of N acetylcysteine (NAC) as an antioxidant in acute OP poisoned. A randomized, controlled, parallel-group trial was conducted in the period from the beginning of January 2022 to the end of June 2022. The study included 56 acute OP poisoned patients admitted to the intensive care unit (ICU) at the Poison Control Center of Ain Shams University Hospitals within 6h after the exposure. The patients were randomly allocated in two equal groups; group (A): received the standard treatment plus NAC in a total dose of 300mg/kg administered intravenously (IV) while group (B) received the standard treatment. Then both groups were compared as regards clinical parameters, laboratory investigations, ECG, and outcomes. Baseline parameters were comparable between the groups. However, NAC treatment significantly elevated concentrations of both serum catalase and glutathione peroxidase levels at 24h, it did not significantly affect the total dose of atropine required, duration of atropine and oximes treatment or need for mechanical ventilation, and length of hospital stay. Mortality was lower in the NAC group (2 out of 28) than the standard treatment-only group (5 out of 28) but the difference was not statistically significant. This trial found that NAC improved antioxidant enzyme levels including serum CAT and GPX but did not affect clinically relevant outcomes.

Effect of Supplementation of Black Seeds (Nigella sativa) on Biomarkers of Oxidative Stress: An Updated Narrative Review of Clinical and Preclinical Studies

Abstract: Increased generation and accumulation of free radicals (reactive oxygen species) and a decrease in the activity of antioxidant systems can result in oxidative stress. In this study, we investigate the antioxidant properties of black seeds (Nigella sativa). To find pertinent papers, searches were conducted using reference lists, Web of Science, Medline/PMC/PubMed, Scopus, Google Scholar, and Science Direct, among other web-based resources. Black seed (Nigella sativa) supplementation has been demonstrated in several preclinical and clinical studies to decrease lipid peroxidation and raise levels of antioxidant enzymes. There are several ways in which black seeds (Nigella sativa) can exhibit antioxidant activity: they can do this by reducing oxidative stress and free radical formation, scavenging superoxide and other reactive oxygen species, inhibiting lipid peroxidation, inhibiting nuclear factor-kB (NF-kB), upregulating genes encoding antioxidant enzymes, lowering malondialdehyde levels, elevating total antioxidant capacity levels, and enhancing antioxidant enzymes like superoxide dismutase, catalase, glutathione peroxidase, and many more. For individuals suffering from chronic conditions including diabetes, hypertension, dyslipidemia, cancer, and many more, black seeds (Nigella sativa) may thus be used as an adjuvant therapy in addition to standard drugs.

Phytochemical composition and neuroprotective potential of Mirabilis jalapa: a review

Four-o’clock plant, scientifically named as Mirabilis jalapa, has gained significant attention for both its ornamental beauty and its medicinal properties, widely utilized as folk remedies. It is a plant grown in the warm climate as a species and showed different pharmacological effects in various therapeutic sectors such as neuroprotective and anxiolytic. Using PubMed, Web of Science Semantic Scholar and Google Scholar and the search terms Mirabilis jalapa phytochemicals; neuroprotection; anxiolytic effect; traditional medicine; flavonoids; alkaloids and neurological disorders literature supporting the medicinal role of Mirabilis jalapa were sourced. The members of Mirabilis jalapa contain flavonoids, alkaloids, triterpenes leaves and saponin through which it provides possibility for the neuroprotection feature. According to research, extracts of the plant can help prevent oxidative stress, a major factor in illnesses including Alzheimer’s and Parkinson’s. Animal studies reveal that components from M. jalapa interfere with Aluminum cytotoxicity by increasing antioxidant enzyme levels, as well as decreasing oxidative damage biomolecules. In addition, the flavonoid constituent has anxiolytic effects because its extract interferes with GABAergic transmission, whereas its effectiveness is comparable to diazepam. Furthermore, the plant has exhibited anticonvulsant effect possibly due to interaction with Ca channel and Glu transmission. Thus, these studies show directions in which the plant works, indicating that it may possess valuable means to treating neurological disorders. However, further clinical trials are needed to understand the exact effects of the plant in human organisms. Mirabilis jalapa therefore, is Discovered to be of great potential as a natural antioxidant and neuroprotective compound as well as anxiolytic remedies.

Parkia biglobosa Jacq. (Locust Bean) leaves and seeds extracts attenuates diabetic-linked cognitive dysfunction in streptozotocin-induced male wistar rats.

Diabetes Mellitus is a metabolic disorder characterized by high blood glucose levels, causing significant morbidity and mortality rates. This study investigated the antidiabetic, neuroprotective, and antioxidant effects of ethanol extracts of Parkia biglobosa (PB) leaves and seeds in streptozotocin (STZ)-induced diabetic rats. The administration of STZ significantly elevated fasting blood glucose levels (FBGL) to 355-400mg/mL compared to 111mg/mL in normal controls, indicating hyperglycemia. Treatment with PB extracts at 100mg/kg and 200mg/kg significantly (p < 0.05) reduced FBGL in a dose-dependent manner. No significant difference was observed between the effects of metformin and PB extracts at 200mg/kg. Cognitive dysfunction, assessed using the Y-maze test, was significantly improved in groups treated with PB extracts (p < 0.05), particularly at 200mg/kg, through inhibition of cholinesterase activity and protection against oxidative damage. Both PB extracts also demonstrated significant inhibition (p < 0.05) of α-amylase and α-glucosidase activity, reducing postprandial hyperglycemia, with a stronger inhibition at 200mg/kg. Additionally, PB extracts significantly increased catalase (CAT) and superoxide dismutase (SOD) activities, reversing the diabetes-induced decline in antioxidant enzyme levels. Monoamine oxidase (MAO) activity, elevated in diabetic conditions, was significantly downregulated by PB treatment, further contributing to neuroprotection. The neuroprotective effects may be attributed to the inhibition of cholinesterase and MAO, which help maintain neurotransmitter levels, alongside the antioxidant properties that mitigate oxidative stress in the brain. These findings suggest that PB extracts could serve as a natural therapeutic agent for diabetes management, with its effects comparable to metformin at higher doses.

Excessive glutathione intake contributes to chemotherapy resistance in breast cancer: a propensity score matching analysis

BackgroundWe aim to explore the impact of excessive glutathione (GSH) intake on chemotherapy sensitivity in breast cancer.MethodsClinicopathological data were collected from 460 breast cancer patients who underwent adjuvant chemotherapy from January 2016 to December 2019 from Zhengzhou University People’s Hospital. The clinicopathological characteristics following GSH treatment were collected and compared with those in Non-GSH group after 1:2 propensity score matching (PSM). Intracellular GSH levels and the expression of antioxidant enzymes (NRF2, GPX4 and SOD1) were evaluated in tumor tissues in 51 patients receiving neoadjuvant chemotherapy.ResultsThe recurrence rate after adjuvant chemotherapy was significantly higher in the GSH group (n = 28, 31.8%) than that in the Non-GSH group (n = 39, 22.2%; P = 0.010). Additionally, patients in the HGSH group (high GSH intake, ≥ 16 days) exhibited an elevated recurrence rate compared to that in the LGSH group (low GSH intake, < 16 days) (n = 15 (46.8%) vs. n = 52 (22.4%); P = 0.003). Cox regression revealed that High GSH intake, Ki67 ≥ 30%, Triple negative and Lymphovascular invasion were independent risk factors of progression after adjuvant chemotherapy. Among patients receiving neoadjuvant chemotherapy, intracellular GSH levels and the expression levels of antioxidant enzymes (NRF2, GPX4 and SOD1) in the resistant patients were substantially higher (P < 0.001).ConclusionsExcessive GSH intake may contribute to chemotherapy resistance in breast cancer, and the levels of intracellular GSH and antioxidant enzymes are elevated in resistant patients after neoadjuvant chemotherapy, indicating that the standardization of GSH intake may assist in reducing chemotherapy resistance.

Antioxidant Response of Vicia faba Plant to Foliar Spray of Green Synthesis Zirconium Oxide Nanoparticles

Previous studies have reported a positive influence of zirconium oxide nanoparticles on plants, the present effort was conducted to investigate the impact of two distinct concentrations of biosynthesized zirconium oxide nanoparticles (75 and 150 mg L-1) on the physiological and antioxidant responses of Vicia faba Plants. Zirconium oxide nanoparticles (ZrO2NPs) were synthesized using Matricaria chamomilla L. plant extracts. And their morphology and extent were determined Using X-Ray Diffraction and Transmission electron microscopy The diffuse reflectance spectra were measured using UV-Vis spectroscopy, the response of V. faba plants to foliar sprays regarding concentrations of (0, 75 and 150 Mg / l-1) M-ZrO2NPs was studied. The largest concentrations of chlorophyll a and b, carotenoids. Total pigments were documented in plants receiving 75mg / l-1 of M-ZrO2NPs associated to the control, secondary metabolite (Phenolics, Flavonoids, and Tannins) were gathered in response to M-ZrO2NPs treatment Glycosides, Terpenoid, Alkaloids (Mayer reagent) and Fuocoumarins. Moreover Tannins, Resins and Saponins, in addition to the levels of enzymatic and non-enzymatic antioxidants, rose by about two-fold in plant exposed to M-ZrO2NPs compared to control plants. The nitrogen, phosphorus, the contents of bean plants in terms also potassium, calcium, zinc, iron were noted in response to two applied quantities of M-ZrO2NPs. Spraying shrubberies by 75 Mg /l-1 of M-ZrO2NPs promoted plant development in addition to the buildup of antioxidants, osmolytes, and secondary metabolites that can help plants cope with the harmful consequences of environmental stress.

Therapeutic potential of diosgenin against methotrexate-induced testicular damage in the rat.

This study evaluated diosgenin effects on methotrexate-induced testicular injury in the rats. A single dose of methotrexate (MTX) (20mg/kg, i.p) was administered, followed by two weeks of diosgenin treatment via gavage starting one day before methotrexate injection. Testicular damage was evaluated through histological examination of seminiferous tubules, as well as analysis of serum testosterone level, oxidative stress and inflammation biomarkers, and antioxidant levels. The results of this study showed that in the MTX-exposed group, oxidative stress indices of malondialdehyde (MDA), reactive oxygen species (ROS), nitrite and indices of inflammation consisting of tumor necrosis factor α (TNFα), and interleukin 6 (IL-6) have a significant increase compared to the control group. Additionally, reductions were observed in antioxidant enzymes including superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH). In addition, testosterone level decreased and signs of testicular damage were observed in the MTX group. Conversely, in the group treated with diosgenin alongside MTX at a dosage of 50mg/kg, there was a significant decrease in oxidative stress markers (MDA, ROS, nitrite) and inflammatory markers (TNFα and IL-6). Moreover, there was a significant increase in the levels of antioxidant enzymes (SOD, CAT, and GSH). Diosgenin appears to have the potential to protect testicular tissue from damage caused by the toxic effects of MTX through the reduction of oxidative stress and inflammation.

Anti-oxidative and immunological role of Cyclocarya paliurus polysaccharide on the liver injury of diabetic rats.

To investigate the effects of Cyclocarya paliurus (C. paliurus) polysaccharide on the liver injury of diabetic rats. Rats were divided into 6 groups, including normal group, model group, control group, low-dose group of C. paliurus polysaccharide treatment, middle-dose group of C. paliurus polysaccharide treatment and high-dose group of C. paliurus polysaccharide treatment. Histological analysis of liver was analyzed using hematoxilin and eosin. Levels of plasma biological parameters and anti-oxidative enzymes were determined by spectrophotometry. Nuclear factor kappa-B-p65 (NF-κB p65), tumor necrosis factor-α (TNF-α), interleukins 6 (IL-6), IL-1β were measured by real-time polymerase chain reaction and enzyme-linked immunosorbent assay. Compared with that of model group, the glucose level of plasma decreased 62.32% (P < 0.01), but glycogen and insulin level increased 1.51 times and 1.27 times in the high-dose group of C. paliurus polysaccharide treatment, respectively. Plasma alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) level decreased 47.47% (P < 0.05), 43.65% (P < 0.05) and 50.51% (P < 0.05) in the high-dose group of C. paliurus polysaccharide treatment, respectively. Superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase level increased 1.16 times (P < 0.01), 71.28% (P < 0.05), 1.29 times (P < 0.01) and 87.46% (P < 0.05) in the high-dose group of C. paliurus polysaccharide treatment, respectively. Enzyme activities of NF-κB, TNF-α, IL-1β and IL-6 were decreased 39.29% (P < 0.05), 51.11% (P < 0.05), 37.42% (P < 0.05) and 36.50% (P < 0.05), respectively. Administration of C. paliurus polysaccharide may play a protecting role for liver injury of diabetic rats through lowering glucose, ALT, AST, ALP level, increasing glycogen and insulin level, enhancing the anti-oxidative ability and down-regulating the inflammatory factors expression.

N-Butanol Fraction of Curcuma Longa (Turmeric) Ameliorates Lead Acetate-Induced Altered Sensory Motor Activity, Oxidative Stress and Histopathological Changes in the Frontal Cortex of Wistar Rat Pups

Background: Lead acetate (Pb) exposure during frontal cortex development is associated with developmental toxicity later in life, causing both morphological and functional alterations. Curcuma longa, however, has been suggested to possess neuroprotective qualities that could lessen these adverse effects. Objective: Assessed the frontal cortex following treatment with Curcuma longa. Materials and Methods: Twenty adult female Wistar rats and ten adult male Wistar rats were matched during the proestrous phase of the estrous cycle in order to mate and create five groups of six (n=6) in a 4:2 (4 females to 2 males) ratio. Gestational day 0 was marked as the confirmation of pregnancy based on if sperm is present and a vaginal plug in the vaginal smear. Four (n=4) pregnant Wistar rats were put together. Group 1 (control) rats were given 2 milliliters per kilogram of distilled water. Pb was given at a dose of 120 mg/kg to Group 2. Group 3 rats were given 120 mg/kg of lead and 100 mg/kg of vitamin C. The animals in Group 4 received 750 mg/kg of Curcuma longa and 120 mg/kg of Pb. The animals in Group 5 rats were given 1500 mg/kg of Curcuma longa and 120 mg/kg of Pb. From gestational day 7 to day 21 (14 days), the medication was administered orally. The animals were allowed to litter naturally. At postnatal day (PND) 1, some pups were euthanized using chloroform inhalation and their brains were harvested for Oxidative stress markers, histology, histochemical assessments. While some pups were kept for Cliff avoidance test at PND 4-7. Results: The study found that lead acetate (Pb) exposure during gestation significantly decreased the mean turning latency in the cliff avoidance test and increased lipid peroxidation (MDA) levels, while decreasing antioxidant enzyme levels (SOD, CAT, GSH) compared to the control group. These neurological and oxidative changes were mitigated by co-administration of Curcuma longa, with a notable improvement in the cliff avoidance test performance and restoration of the altered histological and histochemical markers. The results suggest that Curcuma longa, a natural antioxidant, has neuroprotective properties that can counteract the adverse effects of lead toxicity during gestational development. Conclusion: N-Butanol Fraction of Curcuma Longa ameliorated lead-induced neurotoxicity in rat pups.

Potential of dehydroepiandrosterone and quercetin to ameliorate copper oxide nanoparticles induced hepatotoxicity in albino wistar rats.

The current investigation was designed as an experimental endeavor to explore the protective efficacy of dehydroepiandrosterone (DHEA) and quercetin against hepatotoxicity induced by copper oxide (CuO) nanoparticles. Rats were subjected to CuO nanoparticle intoxication through intraperitoneal injection of 150mg/kg b.w. for three weeks, followed by the administration of the aforementioned antioxidants for an additional three weeks. This study systematically tracked alterations in liver enzymatic activity, antioxidant levels, apoptotic markers, and histopathological changes using the comet assay. CuO nanoparticle-intoxicated rats exhibited a significant increase in serum alanine transaminase aspartate aminotransferase (AST), and bilirubin levels, coupled with a noteworthy reduction in serum albumin. Moreover, there was a marked rise in serum tumor necrosis factor-alpha levels, concomitant with a significant decline in serum hepatocyte growth factor (HGF). Caspase-3 and Bax mRNA levels in the serum showed a substantial increase, while serum Bcl-2 mRNA levels witnessed a significant decrease. Liver tissue levels of malondialdehyde (MDA) and nitric oxide (NOx) experienced a significant elevation, and DNA damage was observed through the comet assay. Histopathological examination of the liver tissue substantiated these aforementioned findings. Administration of the antioxidants DHEA or quercetin, either individually or in combination, mitigated the parameters of hepatotoxicity to varying extents. In summary, the hepatic genotoxicity induced by CuO nanoparticles demonstrated improvement following the administration of either DHEA or quercetin. Additionally, their combined administration exhibited a more potent protective potential.