Level Of Aberrations Research Articles

Depth of focus as a function of spherical aberration using adaptive optics in pseudophakic subjects.

To measure visual acuity at three different defocus planes in pseudophakic subjects with varying levels of spherical aberration induced by an adaptive optics visual simulator. The study aimed to simulate Extended Depth of Focus (EDOF) intraocular lenses (IOLs). Private hospital (IMO, Barcelona, Spain). Observational case series; modelling theory. Through-focus visual acuity was measured in 26 pseudophakic subjects (age 63 ± 10 years old) with an adaptive optics visual simulator optimized for clinical use (VAO, Voptica SL, Murcia, Spain). Measurements were made under five different conditions of induced negative spherical aberration: 0, -0.07, -0.15, -0.23 and -0.30 μm (pupil diameter: 4.5 mm). Results were also modelled using ray tracing simulations. On average, depth of focus was extended when spherical aberration increased from -0.07 to -0.15 μm (4.5 mm pupil diameter). Some indivisuals (27%) experienced improved depth of focus with higher magnitudes of spherical aberration, while others (23%) exhibited no benefit from increased (negative) SA, as visual acuity dropped below acceptable levels. Depth of focus calculations based on ray tracing showed general agreement with the measurements. The visual conditions of EDOF IOLs were artificially recreated in a population of pseudophakic patients implanted with a monofocal IOL. The variability seen across subjects in visual acuity at different defocus planes suggests that visual simulators might be capable of screening subjects for suitability and tolerability of these advanced technology lenses.

Can volatile sulphides act as fumigant or contact toxicant in cowpea weevil, Callosobruchus maculatus F.?: a comparative analysis

For understanding the toxic action of volatile sulphides, we have evaluated the insecticidal efficacy of sulphide mixture (diallyldisulfide, diallylsulfide and diallyltrisulfide) against Callosobruchus maculatus F. adults by fumigant toxicity (FT) and contact toxicity (CT) bioassays. Further, diffusional characteristics of sulphides inside the treatment vials were analysed by GC-FPD and microstructural impacts on treated beetles were observed using SEM. In the comparative analysis, maximum level of insecticidal toxicity (0.88 µl/L of LC50), residue persistence (32.28 ppm/adult) and microstructural aberration in beetle elytra was observed in CT compared to FT bioassay (1.60 µl/L of LC50 and 22.67 ppm/adult of residue). Whereas, the GC-FPD based diffusion analysis indicated even distribution of volatiles inside the vials in both FT and CT bioassays. The study results suggests that volatiles act as fumigant/vapours in both bioassays with respect to even diffusion and toxicity occurs based on insect–volatile contacts inside the treatment chambers.

STUDY OF CHROMOSOME STABILITY OF HUMAN SOMATIC CELLS IN THE DEVELOPMENT OF THE SECONDARY RADIATION-INDUCED BYSTANDER EFFECT.

To establish the level of chromosomal instability in human peripheral blood lymphocytes during thedevelopment of secondary radiation-induced bystander effect. Human peripheral blood lymphocytes; culture of human non-small-cell lung cancer cell lineA549 (irradiated in vitro by 137Cs in a dose of 0.50 Gy/unirradiated). Cytogenetic analysis of uniformly colored chromosomes of human peripheral blood lymphocytes with determination of the frequency of all types of aberrations. The mean group frequency of chromosome aberrations (4.75 per 100 cells) in human peripheral blood lymphocytes co-cultivated with conditioned culture medium from bystander cells of human non-small cell lung cancercell lines A-549 exceeded the control (1.60 per 100 cells) (p < 0.001) due to an increase in the frequency of singlefragments from 1.20 to 3.95 per 100 cells (p < 0.001). The mean group frequency of chromosome aberrations andchromatid-type aberrations in human peripheral blood lymphocytes during the development of primary (5.80 and4.35 per 100 cells, respectively) and secondary (4.75 and 3.95 per 100 cells, respectively) radiation-induced bystander effect did not significantly differ from each other (p > 0.05). Under the conditions of co-cultivation of human peripheral blood lymphocytes with conditioned culture medium from bystander cells of human non-small cell lung cancer A-549, a secondary radiation-induced bystander effect was registered, the cytogenetic manifestation of which was an increase in the level of chromosomalinstability due to the rise in the frequency of chromatid-type aberrations (single fragments). The levels of chromatid-type chromosome aberrations during the development of the primary and secondary radiation-inducedbystander effect did not differ significantly. The development of the secondary radiation-induced bystander effectdid not affect the induction of chromosomal-type aberrations.

Vitamin B12 Protects Against Genotoxicity Induced by Cisplatin.

Cisplatin is an effective synthetic chemotherapeutic drug used for cancer treatment. Vitamin B12 has been shown to possess anti-genotoxic activity. This study aimed to investigate the effect of vitamin B12 on chromosomal damage induced by cisplatin. The level of sister chromatid exchanges (SCEs) and chromosomal aberrations (CAs) were measured in cultured human blood lymphocytes treated with cisplatin and/or vitamin B12. The results showed a significantly elevated frequency of CAs and SCEs of cisplatin-treated cultures compared to the control (P < 0.05). The CAs and SCEs induced by cisplatin were significantly lowered by pretreatment of cell cultures with vitamin B12. In addition, cisplatin caused a slight reduction in the mitotic index (MI), while vitamin B12 did not modulate the effect of cisplatin on MI. Vitamin B12 can protect human lymphocytes against genotoxicity associated with cisplatin.

Accommodating version of a schematic eye for emmetropia and myopia.

To develop an accommodating, wide-angle, schematic eye for emmetropia and myopia in which spectacle refraction and accommodation level are input parameters. The schematic eye is based on an earlier unaccommodated refraction-dependent eye for myopia developed by Atchison in 2006. This has a parabolic gradient index lens and parameters derived from biometric and optical measurements on young adults. Several parameters are linearly dependent upon spectacle refraction (anterior radius of curvature of the cornea, axial length and vertex radii of curvature and conic asphericities of a biconic retina). The new accommodated schematic eye incorporates accommodation-dependent changes in several lens-related parameters. These changes are based on literature values for anterior chamber depth, lens thickness, vitreous chamber depth, lens surface radii of curvature and lens front surface asphericity. A parabolic variation of refractive index with relative distance from the lens centre is retained, with the same edge and centre refractive indices as the earlier model, but the distribution has been manipulated to maintain focus near the retina for the emmetropic case at 0 and 4 D accommodation. The asphericity of the lens back surface is changed so that spherical aberration and peripheral refraction approximately match typical literature trends. The model is used to compare spherical aberration and peripheral refraction in eyes with up to 4 D of myopia and 4 D of accommodation. The levels of spherical aberration in the unaccommodated schematic eyes are similar to literature values for young adults, but the changes in spherical aberration with accommodation are approximately two-thirds of that found in an experimental study. As intended, peripheral refractions in the accommodated schematic eyes are similar to those of their unaccommodated counterparts. The wide-angle model extends the range of schematic eyes to include both refraction and accommodation as variable input parameters. It may be useful in predicting aspects of retinal image quality.

Cytogenetic Violations in Blood Lymphocytes of Macaca Mulatta Monkeys in the Long Term after Irradiation with Accelerated Krypton Ions

A study has been performed of cytogenetic violations that occur in blood lymphocytes of Macaca mulatta monkeys after local exposure of the hippocampal region to accelerated 78Kr ions at a dose of 3 Gy. Analysis revealed a low level of chromosomal aberrations in the control group of monkeys. The number of cells with chromosomal aberrations did not exceed 1.67٪. The main fraction was chromatid-type aberrations. A cytogenetic analysis of peripheral blood lymphocytes of monkeys exposed to accelerated krypton ions revealed the maximum level of chromosomal aberrations 24 h after exposure, which exceeded the control level by 2.7 times. By day 96, the number of aberrations decreased 1.7-fold. However, it still exceeded the control level. By 460 days, some animals showed a slight increase in the number of aberrations compared to the previous observation period. In the long term, a number of irradiated animals showed deviations from the standard behavior of monkeys. Data on chromosomal aberrations in blood lymphocytes of monkeys and humans after in vitro irradiation with 170 MeV protons are compared. The relevance of this research is due to the preparation of interplanetary manned flights.

Higher Order Aberrations in Patients with Unilateral Nasolacrimal Duct Obstruction.

To compare corneal higher order aberrations (HOAs) in patients with epiphora caused by unilateral primary acquired nasolacrimal duct obstruction (PANDO) and their fellow eyes. A total of 122 eyes of 61 patients with unilateral PANDO were included. Affected eyes were named as group 1 and contralateral eyes as group 2. The Munk score and fluorescein dye disappearance test (FDDT) were assessed. Corneal topographic and HOAs measurements were taken by Sirius topography. There were 47 female and 14 male patients. The mean keratometry was 43.84 ± 1.4 in group 1 and 43.96 ± 1.4 in group 2 (p = 0.73). The mean corneal topographic astigmatism was 0.81 ± 0.7 in group 1 and 0.78 ± 0.5 in group 2 (p = 0.57). The mean coma-like aberration was 0.19 ± 0.2 in group 1 and 0.10 ± 0.1 in group 2 (p = 0.03). The mean spherical-like aberration was 0.16 ± 0.1 in group 1 and 0.11 ± 0.1 in group 2 (p = 0.04). The mean total corneal HOA was 0.30 ± 0.5 in group 1 and 0.23 ± 0.2 in group 2 (p = 0.04). The mean Munk score was 3.47 ± 1.0 in group 1. There was a positive correlation between the Munk score and both coma-like aberration (r = 0.53, p = 0.003) and total corneal HOAs (r = 0.44, p = 0.010). The mean FDDT grade was 2.60 ± 0.1 in group 1. A positive correlation was observed between the FDDT grade and both coma-like aberration (r = 0.48, p = 0.001) and total corneal HOAs (r = 0.33, p = 0.001). Epiphora in patients with PANDO can affect corneal HOAs. As patients' symptoms and tear pooling were increased as corneal HOA levels increased.

Differential Lucy-Richardson-Rosen algorithm for near diffraction-limited image restoration assisting by a wavefront sensor

Lucy-Richardson-Rosen algorithm (LR2A) is one of the most important tools for image restoration, especially in computer vision, target imaging and so on. However, the performance of the LR2A can be affected by large optical aberration and imaging noise and even failure under severe environment. To address the mentioned problem, we proposed a new Differential Lucy-Richardson-Rosen Algorithm(D-LR2A) to reduce the influence of optical aberrations and detection noise with advantages of faster speed of convergence and better restoration quality. In the modified optical configuration, a Hartmann-Shack wavefront sensor is employed to measure optical aberration and estimate accurately the Point Spread Function (PSF) of the optical imaging system. The numeric and experimental results show that the proposed method can be used to restore images with large optical aberration and worse noise level. Besides that, it also has faster speed, better restoration quality, and thus easier application as compared to those of LR2A

REV1 coordinates a multi-faceted tolerance response to DNA alkylation damage and prevents chromosome shattering in Drosophila melanogaster.

When replication forks encounter damaged DNA, cells utilize damage tolerance mechanisms to allow replication to proceed. These include translesion synthesis at the fork, postreplication gap filling, and template switching via fork reversal or homologous recombination. The extent to which these different damage tolerance mechanisms are utilized depends on cell, tissue, and developmental context-specific cues, the last two of which are poorly understood. To address this gap, we have investigated damage tolerance responses in Drosophila melanogaster. We report that tolerance of DNA alkylation damage in rapidly dividing larval tissues depends heavily on translesion synthesis. Furthermore, we show that the REV1 protein plays a multi-faceted role in damage tolerance in Drosophila. Larvae lacking REV1 are hypersensitive to methyl methanesulfonate (MMS) and have highly elevated levels of γ-H2Av (Drosophila γ-H2AX) foci and chromosome aberrations in MMS-treated tissues. Loss of the REV1 C-terminal domain (CTD), which recruits multiple translesion polymerases to damage sites, sensitizes flies to MMS. In the absence of the REV1 CTD, DNA polymerases eta and zeta become critical for MMS tolerance. In addition, flies lacking REV3, the catalytic subunit of polymerase zeta, require the deoxycytidyl transferase activity of REV1 to tolerate MMS. Together, our results demonstrate that Drosophila prioritize the use of multiple translesion polymerases to tolerate alkylation damage and highlight the critical role of REV1 in the coordination of this response to prevent genome instability.

Werner helicase interacting protein 1 contributes to G-quadruplex processing in human cells

Genome replication is frequently impeded by highly stable DNA secondary structures, including G-quadruplex (G4) DNA, that can hinder the progression of the replication fork. Human WRNIP1 (Werner helicase Interacting Protein 1) associates with various components of the replication machinery and plays a crucial role in genome maintenance processes. However, its detailed function is still not fully understood. Here we show that human WRNIP1 interacts with G4 structures and provide evidence for its contribution to G4 processing. The absence of WRNIP1 results in elevated levels of G4 structures, DNA damage and chromosome aberrations following treatment with PhenDC3, a G4-stabilizing ligand. Additionally, we establish a functional and physical relationship between WRNIP1 and the PIF1 helicase in G4 processing. In summary, our results suggest that WRNIP1 aids genome replication and maintenance by regulating G4 processing and this activity relies on Pif1 DNA helicase.

Assessment of Wavefront Aberrations in Children with Myopia Against the Background of Ocular Surface Disorders in Combination with Computer Vision Syndrome Digital and Eye Strain

Purpose: to assess the state of the wavefront in children with myopia, who have various manifestations of digital eye strain and symptoms of ocular surface disorders, and to evaluate the diagnostic significance of wavefront criteria for assessing the state of the ocular surface.Patients and methods. The study involved 76 children (152 eyes) with myopia aged 8 to 18 years who used gadgets and computers for more than 2 hours a day. All patients used glasses as a method of optical correction and were constantly worn. The patients were divided into 2 groups: 1st with symptoms of ocular surface disorder, 2nd — comparison group. Subjective signs were studied using the online questionnaire “State of the ocular surface”. Using the “Keratograph 5M Oculus” the following parameters were assessed: non­invasive tear film breakup time (NTBR), including the first tear film breakup time, average tear film breakup time, breakup time gradient and maximum tear film breakup zone, the same device was used to perform infrared meibography and study of the lipid layer of the tear film. All patients underwent wavefront aberrometry in a darkened room without cycloplegia.Results. It was found that in the group of patients with the presence of subjective phenomena of disturbance of the state of the ocular surface, identified using the online questionnaire “State of the ocular surface”, rotor aberrations were statistically significantly higher than in the comparison group. At the same time, the indicators of higher­order corneal aberrations have comparable values. Correlation analysis between indicators of the state of the ocular surface and parameters of the wavefront in the group of children with disturbances of the state of the ocular surface showed that a higher level of corneal aberrations corresponds to a higher number of points on the questionnaire of disturbances of the ocular surface and the state of visual comfort, in turn, when comparing data on non­invasive tear film breakup time, a negative correlation was established, which means that with a higher number of points on the questionnaire, and therefore, with a more significant degree of impairment of the ocular surface, the tear film breakup time was shorter.Conclusion. The structure of the wavefront in children with myopia against the background of a disorder of the ocular surface in combination with CVS and digital eye strain is significantly different from the structure of the wavefront in the comparison group.

Molecular analysis for ovarian cancer detection in patient-friendly samples

BackgroundHigh ovarian cancer mortality rates motivate the development of effective and patient-friendly diagnostics. Here, we explored the potential of molecular testing in patient-friendly samples for ovarian cancer detection.MethodsHome-collected urine, cervicovaginal self-samples, and clinician-taken cervical scrapes were prospectively collected from 54 patients diagnosed with a highly suspicious ovarian mass (benign n = 25, malignant n = 29). All samples were tested for nine methylation markers, using quantitative methylation-specific PCRs that were verified on ovarian tissue samples, and compared to non-paired patient-friendly samples of 110 age-matched healthy controls. Copy number analysis was performed on a subset of urine samples of ovarian cancer patients by shallow whole-genome sequencing.ResultsThree methylation markers are significantly elevated in full void urine of ovarian cancer patients as compared to healthy controls (C2CD4D, P = 0.008; CDO1, P = 0.022; MAL, P = 0.008), of which two are also discriminatory in cervical scrapes (C2CD4D, P = 0.001; CDO1, P = 0.004). When comparing benign and malignant ovarian masses, GHSR shows significantly elevated methylation levels in the urine sediment of ovarian cancer patients (P = 0.024). Other methylation markers demonstrate comparably high methylation levels in benign and malignant ovarian masses. Cervicovaginal self-samples show no elevated methylation levels in patients with ovarian masses as compared to healthy controls. Copy number changes are identified in 4 out of 23 urine samples of ovarian cancer patients.ConclusionsOur study reveals increased methylation levels of ovarian cancer-associated genes and copy number aberrations in the urine of ovarian cancer patients. Our findings support continued research into urine biomarkers for ovarian cancer detection and highlight the importance of including benign ovarian masses in future studies to develop a clinically useful test.

Risk Factors for Corneal Monochromatic Aberrations and Implications for Multifocal and Extended Depth-of-Focus Intraocular Lens Implantation.

To discuss factors influencing corneal aberrations that might influence the optical quality after intraocular lens (IOL) implantation. PubMed and Scopus were the main resources used to search the medical literature. An extensive search was performed to identify relevant articles concerning factors influencing the level of corneal aberrations as of August 27, 2023. The following keywords were used in various combinations: corneal, aberrations, defocus, astigmatism, spherical aberration, coma, trefoil, quadrafoil, intraocular lens, and IOL. Conclusive evidence is lacking regarding the correlation between age and changes in corneal aberrations. Patients with astigmatism have greater corneal higher-order aberrations than those with minimal astigmatism, particularly concerning trefoil and coma. Increased levels of corneal higher-order aberrations are noted following contact lens wear, in patients with dry eye disease, and with pterygium. Increased higher-order aberrations have been reported following corneal refractive surgery and for 3 months following trabeculectomy; regarding intraocular lens surgery, the results remain controversial. Several factors influence the level of corneal higher-order aberrations. Multifocal and extended depth-of-focus IOLs can share similarities in their optical properties, and the main difference arises in their design and performance with respect to spherical aberration. Preoperative evaluation is critical for proper IOL choice, particularly in corneas with risk of high levels of aberrations. [J Refract Surg. 2024;40(6):e420-e434.].

Higher-order Aberrations — the Choice of Optimal Intraocular Correction in the Surgical Treatment of Cataracts. Review of the Literature

The cornea is the most powerful refractive element of the eye and plays a fundamental role in the quality of vision. Imperfection of corneas shape leads to the focusing errors formation, known as optical aberrations, which are responsible for visual performance deterioration. Understanding and assessing wavefront errors in IOL selection and calculation is great importance to achieve maximum optical outcome in the postoperative period. The article presents literature data of the effect of higher-order aberrations on the vision quality in unoperated eyes, changes of the wavefront in the eyes after cornea surgical interventions, the effect of various types of IOLs (spherical, aspherical, multifocal and EDOF) on the total error of the eye wavefront, recommendations at their choice with different severity levels of optical aberrations, as well as promising areas of research on this issue.

Ocular parameters associated with visual performance of enhanced monofocal intraocular lens

BackgroundAn enhanced monofocal intraocular lenses (IOLs) (Tecnis Eyhance ICB00 and Tecnis Eyhance Toric DIU) has been developed to enhance intermediate vision while avoiding the disadvantages of multifocal IOLs. Although many studies have demonstrated the improvement of intermediate visual acuity with enhanced monofocal IOLs, it is not known specifically for which patients these IOLs should be recommended or avoided. In this study, we aim to find out which ocular parameters affect vision performance and photic phenomenon of ICB00 or DIU at different distances.MethodsPatients who underwent cataract surgery with ICB00 or DIU, performed by a single surgeon, were included. Before surgery, the patients’ age, gender, axial length, anterior chamber depth, spherical aberration Z (4,0), vertical coma, horizontal coma, angle kappa (κ), angle alpha (α), and other ocular parameters were investigated. One month after surgery, uncorrected near visual acuity (UNVA at 40 cm), uncorrected intermediate visual acuity (UIVA at 66 cm), uncorrected distance logMAR visual acuity (UDVA), IOL decentration, and quality of vision (QoV) questionnaires were conducted.ResultsA total of 43 patients (58 eyes) were included. The results of the univariate linear regression analyses showed a negative correlation between spherical aberration and logMAR UNVA and UIVA (p = 0.003, β=-0.51 and p = 0.018, β=-0.23, respectively) and a positive correlation between angle α and logMAR UIVA (p = 0.036, β = 0.19). Deeper anterior chamber depth (ACD) was associated with poorer total QoV (p = 0.018, β = 14.43), particularly in glare, halo, blur, and fluctuation perception. A higher degree of IOL decentration tended to decrease UNVA and UIVA (Pearson correlation coefficient, r = 0.336 and r = 0.221, respectively); however, no significant effect was observed on UDVA (Pearson correlation coefficient, r = 0.042).ConclusionsIn enhanced monofocal IOLs, a higher level of spherical aberration is associated with better performance in UNVA and UIVA, whereas a larger angle α has a negative impact. A deeper ACD negatively affects the QoV.

Comparison of visual quality after wavefront-guided LASIK in patients with different levels of preoperative total ocular higher-order aberrations: a retrospective study.

To compare the visual quality after wavefront-guided femtosecond LASIK (WFG FS-LASIK) in patients with different levels of preoperative total ocular higher-order aberrations to guide clinical decision-making regarding patient selection and treatment strategies. This study included 112 right eyes of 112 patients who previously underwent WFG FS-LASIK for correcting myopia and myopic astigmatism. The patients were divided into two groups based on the mean values of preoperative total ocular HOAs (0.30 ± 0.09 µm): HOA ≤ 0.3 and > 0.3 groups. The visual acuity, manifest refraction, corneal Strehl ratio (SR), root mean square (RMS) of corneal and ocular aberrations, and area under the log contrast sensitivity function (AULCSF) of both groups were compared preoperatively and at 1, 3, 6, and 12 months postoperatively. The induced ocular HOAs and coma (Δ = 1 mo - Preop) were significantly lower in the HOAs > 0.3 group than in the HOAs ≤ 0.3 group (ΔHOAs: 0.39 ± 0.19 vs. 0.29 ± 0.18 μm, t = 2.797, P = 0.006; Δ coma: 0.30 ± 0.19 vs. 0.20 ± 0.21 μm, t = 2.542, P = 0.012). In the HOAs > 0.3 group, ΔHOAs were negatively correlated with the preoperative ocular HOAs (r = -0.315, P = 0.019). In the HOAs ≤ 0.3 group, the regression equation for Δ HOAs = 0.098 + 0.053 |SE| (F = 21.756, P < 0.001). In the HOAs > 0.3 group, the regression equation for ΔHOAs = 0.534 - 1.081 HOAs + 0.038|Sphere| (F = 7.954, P = 0.001). The postoperative uncorrected distance visual acuity, spherical equivalent, corneal aberrations, SR and AULCSF of both groups were similar (all P > 0.05). Furthermore, the ocular aberrations were not significantly different between both groups at 3, 6, and 12 months postoperatively (all P > 0.05). In addition, compared with the preoperative period, the AULCSF of both groups were significantly increased in the postoperative period (all P < 0.05). The induced ocular HOAs and coma in HOAs > 0.3 group were lower. However, both groups achieved equivalent and excellent visual quality after WFG FS-LASIK. WFG FS-LASIK may provide significant visual benefits for a wider range of patients.

Wavefront characteristics in amblyopia of different etiologies in children

Forty-five patients (90 eyes) were examined. All patients were divided into five groups: 1 - with dysbinocular amblyopia; 2 - with refractive amblyopia; 3 - with anisometropic amblyopia; 4 - with relative amblyopia due to congenital myopia; 5 (control) - fellow eyes without amblyopia. Aberrometry was performed using the OPD-Scan III device (Nidek, Japan). Fixation parameters were studied on the MP-3 microperimeter (Nidek, Japan). Correlation analysis was performed using Pearson's linear correlation coefficient (r). In amblyopia associated with congenital myopia, a significant increase in corneal and internal aberrations RMS, Total HOA, astigmatism (V) (0.65±0.26; 1.01±0.31; 4.22±1.17; -2.17±0.72; 0.86±0.3, respectively; control group - 0.44±0.19; 0.58±0.27; 1.0±0.75; -0.94±0.89; 0.47±0.65) and internal spherical aberration (0.06±0.02; control group - 0.04±0.03) was found. In dysbinocular amblyopia, a significant increase in internal aberrations Trefoil (V) and Coma (H) (0.75±0.52 and 0.17±0.35, respectively; control group - 0.05±0.28 and -0.07±0.21) was found, which correlated with a decrease in fixation density in the 2° ring (r= -0.40, r= -0.41). The increased level of higher-order aberrations in amblyopia associated with congenital myopia is due to the anatomical and optical features of the eyes. The increase in internal aberrations Trefoil (V) and Coma (H) in dysbinocular amblyopia is associated with a mismatch of the optical elements of the eye due to impaired fixation, i.e., it is not the cause, but the consequence of amblyopia.

Risk factors associated with visual performance of enhanced monofocal intraocular lens

Aims/Purpose: To investigate the ocular parameters that affect visual acuity and quality with an enhanced monofocal intraocular lens (Tecnis Eyhance ICB00 and Tecnis Eyhance Toric DIU).Methods: Patients who underwent cataract surgery with ICB00 or DIU by a single surgeon were included. Prior to surgery, the patients' age, gender, axial length, anterior chamber depth, spherical aberration Z (4,0), vertical coma, horizontal coma, angle κ, angle α and other ocular parameters were investigated. At 1 month after surgery, uncorrected near (40 cm, UNVA), intermediate (66 cm, UIVA) and distance logMAR visual acuity (UDVA), IOL decentration amount and a quality of visual acuity (QoV) questionnaire were conducted.Results: A total of 43 patients with 58 eyes were enrolled in this study. The results of univariate linear regression analyses showed a negative correlation between spherical aberration and logMAR UNVA and UIVA (p = 0.003, β = −0.51 and p = 0.018, β = −0.23, respectively), and a positive correlation between angle α and logMAR UIVA (p = 0.036, β = 0.19). Deeper anterior chamber depth was associated with poorer total QoV (p = 0.018, β = 14.43), particularly in the glare, halo, blurred and fluctuation perception. A higher amount of IOL decenteration showed a tendency to decrease UNVA and UIVA (Pearson correlation coefficient, r = 0.336, r = 0.221), but there was no significant effect on UDVA (Pearson correlation coefficient, r = 0.042).Conclusions: In enhanced monofocal intraocular lenses, a higher level of spherical aberration is associated with better performance in near and intermediate visual acuity, while a larger angle α has a negative impact. A deeper anterior chamber depth negatively effects on the quality of visual acuity. Moreover, as the amount of IOL decentration increases, there is a tendency for a decrease in near and intermediate visual acuity.

Concurrent TERT Amplifications in EGFR Mutated Lung Adenocarcinoma are Associated with Higher Levels of Copy Number Aberrations - A Hypothesis-Generating Study

Concurrent TERT Amplifications in EGFR Mutated Lung Adenocarcinoma are Associated with Higher Levels of Copy Number Aberrations - A Hypothesis-Generating Study

Study of antimutagenic activity of medicinal plant infusions Crataegus sanguinea Pall. family Rosaceae in plant test systems

Medicinal plants, integral to traditional medicine systems, are rich sources of biologically active substances that benefit physiological and biochemical processes within living organisms. Amongst these activities, antimutagenic and genoprotective properties stand out, offering mitigation against genotoxic effects induced by adverse environmental factors on genetic material. This study delves into the mutagenic and antimutagenic capacities of aqueous and alcoholic infusions derived from Crataegus sanguinea Pall. (Rosaceae family), utilising Hordeum vulgare L. as a plant-based test subject. The assessment employed the metaphase chromosome analysis technique. Results indicate that these infusions exhibit no mutagenic activity, with the level of chromosomal aberrations in barley seeds treated with these infusions not exceeding the natural mutation rate in a statistically significant manner. When infusions were combined with Methyl methanesulfonate (positive control) exposure – irrespective of exposure sequence – a statistically significant attenuation in MMS-induced mutagenesis was observed (p&lt;0.01). A 56-60% reduction quantified the antimutagenic efficacy of C.sanguinea infusions. This metric underscores the infusions’ capability to inhibit MMS-induced mutagenesis by 50–60%, positioning these water and alcohol-based extracts of common hawthorn as viable candidates for safeguarding against chemically induced mutagenic factors.