Root Level Research Articles

Bacillus velezensis BE2 controls wheat and barley diseases by direct antagonism and induced systemic resistance.

Wheat and barley rank among the main crops cultivated on a global scale, providing the essential nutritional foundation for both humans and animals. Nevertheless, these crops are vulnerable to several fungal diseases, such as Septoria tritici blotch and net blotch, which significantly reduce yields by adversely affecting leaves and grain quality. To mitigate the effect of these diseases, chemical fungicides have proven to be genuinely effective; however, they impose a serious environmental burden. Currently, biocontrol agents have attracted attention as a sustainable alternative to fungicides, offering an eco-friendly option. The study aimed to assess the efficacy of Bacillus velezensis BE2 in reducing disease symptoms caused by Zymoseptoria tritici and Pyrenophora teres. This bacterium exhibited significant antagonistic effects in vitro by suppressing fungal development when pathogens and the beneficial strain were in direct confrontation. These findings were subsequently confirmed through microscopic analysis, which illustrated the strain's capacity to inhibit spore germination and mycelial growth in both pathogens. Additionally, the study analysed the cell-free supernatant of the bacterium using UPLC-MS (ultra-performance liquid chromatography-mass spectrometry). The results revealed that strain BE2 produces, among other metabolites, different families of cyclic lipopeptides that may be involved in biocontrol. Furthermore, the beneficial effects of strain BE2 in planta were assessed by quantifying the fungal DNA content directly at the leaf level after bacterization, using two different application methods (foliar and drenching). The results indicated that applying the beneficial bacterium at the root level significantly reduced pathogens pressure. Finally, gene expression analysis of different markers showed that BE2 application induced a priming effect within the first hours after infection. KEY POINTS: • BE2 managed Z. tritici and P. teres by direct antagonism and induced systemic resistance. • Strain BE2 produced seven metabolite families, including three cyclic lipopeptides. • Application of strain BE2 at the root level triggered plant defense mechanisms.

A Community Based Study on Various Aspects of Breast-Feeding Practices from Central India

Background: For optimum growth and development of newborns breastfeeding is of utmost importance.Identification of breastfeeding practise gaps need to be identified and filled. This study was undertaken to explorethe breastfeeding practices, influence of literacy and prevailing cultural factors on different aspects of breastfeeding.Materials and Methods: Complete line listing of 576 breastfeeding women in the study area was done. Thenthe study subjects were interviewed by making house to house visits. Data was gathered on the participant’sdemographics, breastfeeding knowledge, and practices. Privacy was maintained while conducting the interview.Anonymity and confidentiality were ensured to the study participants.Results: Data of 550 study participants was analyzed and presented here. 377 (68.5%) could not practice timelyinitiation of breast feeding. Most common reason (264, 70%) for the same was insufficient knowledge about it.Exclusive breast feeding was practiced by merely 44 (8%) subjects. Not good for health of newborn (108, 36.7%),not easily digested by the newborn (thick) (85, 28.9%) and myths prevalent in society (64, 21.8%) were three mostcommon reasons for discarding colostrum by mothers. Lower socio-economic status, muslim religion and jointfamily were found to be associated with practice of exclusive breastfeeding.Conclusion: The issue of lower prevalence of early breastfeeding initiation and exclusive breastfeeding continuesto persist in the study area. Still, colostrum is being discarded. Tailored and region specific health promotionactivities must be intensified in this regard by grass root level health care workers.

A multi-criteria drought resistance assessment of temperate Acer, Carpinus, Fraxinus, Quercus, and Tilia species

A rapidly warming climate with growing frequency of hot droughts urges Central Europe’s forestry sector to adapt to increasing climatic stress. One option is to choose native minor timber species with assumed higher stress tolerance; yet, information on the drought resistance of many species is scarce. We examined the drought resistance of adult trees of Norway maple (Acer platanoides), European hornbeam (Carpinus betulus), Common ash (Fraxinus excelsior) and Little-leaved lime (Tilia cordata) at leaf, branch, stem and root levels, combining studies on leaf water status, branch xylem hydraulics, fine root vitality and radial stem growth, for deriving an evidence-based drought resistance ranking of the species. Results were compared to Sessile oak (Quercus petraea), a fairly drought-resistant major timber species. All species showed constant growth rates despite increasing climatic aridity, indicating low climate vulnerability. Foliage loss after the severe 2018/19 drought increased in the sequence Quercus < Fraxinus < Acer < Tilia < Carpinus. The water potential at leaf turgor loss (PTLP) was no suitable indicator of the species’ climate-sensitivity of growth or drought-induced foliage loss. The growth performance of Tilia demonstrates that some angiosperm trees can achieve a fairly high degree of drought resistance through plant-internal water storage and high leaf tissue elasticity, despite a small hydraulic safety margin and high PTLP. Drought resistance as deduced from growth performance and defoliation after severe drought decreased in the sequence Quercus > Fraxinus & Acer > Tilia > Carpinus. We conclude that Acer, Carpinus, and Tilia (and Fraxinus, despite being often Hymenoscyphus-infected) are suitable timber species for Central Europe’s forestry sector in a drier and warmer climate.

Post retention strength of apical and conventional coating obturation methods using bioceramic sealer: a laboratory investigation

BackgroundOnce bioceramic sealer (BCS) enters the dentinal tubules, it cannot be reliably removed. BCS-occupied dentinal tubules reduce fibre post retention strength. Coating gutta-percha with BCS only on the apical portion may improve post retention strength due to increased retention strength between the dentin and resin cement interface. The aim of the study was to test this hypothesis.MethodsRoot canals of 27 extracted human mandibular premolars were instrumented and randomly assigned to three obturation methods: conventional coating (CC), non-coating (NC), and apical coating (AC). The root canals were obturated with gutta-percha to 4 mm from the working length under an operating microscope. After the BCS was completely set, post spaces were prepared, and quartz fibre posts were cemented. The apical 4.5 mm of the roots were removed. Two samples were prepared at the apical, middle, and coronal root levels (one for scanning electron microscope (SEM) study and another for the push out bond strength (PBS) test). After the PBS test, the samples were examined with a stereo microscope to determine the failure mode: dentine-cement (DC), post-cement (PC) and mixed. The PBS data were analysed by One way ANOVA for the specific obturation method effects. Repeated ANOVA was used for the specific effects of the root levels on PBS in different obturation methods.ResultsAt all three root levels, more continuous hybrid layers and denser resin tags were found in the NC and AC than the CC group. The AC and NC groups’ PBS was significantly higher than the CC group at the apical 1/3 (p = 0.002 and p = 0.001) and coronal 1/3 (p = 0.016 and p = 0.041). The PBS in the CC group at the middle 1/3 was significantly higher than the apical 1/3 (p = 0.022). DC failure mode was most commonly found in the CC group, while PC failure mode was found most frequently in the NC and AC groups.ConclusionsThe apical coating obturation method significantly increased PBS over the conventional coating method, potentially reducing fiber post dislodgement. However, this study was only preliminary. Clinical studies are required to confirm the results.

Molecular identification and functional characterization of a transcription factor GeRAV1 from Gelsemium elegans

BackgroundGelsemium elegans is a traditional Chinese medicinal plant and temperature is one of the key factors affecting its growth. RAV (related to ABI3/VP1) transcription factor plays multiple roles in higher plants, including the regulation of plant growth, development, and stress response. However, RAV transcription factor in G. elegans has not been reported.ResultsIn this study, three novel GeRAV genes (GeRAV1-GeRAV3) were identified from the transcriptome of G. elegans under low temperature stress. Phylogenetic analysis showed that GeRAV1-GeRAV3 proteins were clustered into groups II, IV, and V, respectively. RNA-sequencing (RNA-seq) and real-time quantitative PCR (qRT-PCR) analyses indicated that the expression of GeRAV1 and GeRAV2 was increased in response to cold stress. Furthermore, the GeRAV1 gene was successfully cloned from G. elegans leaf. It encoded a hydrophilic, unstable, and non-secretory protein that contained both AP2 and B3 domains. The amino acid sequence of GeRAV1 protein shared a high similarity of 81.97% with Camptotheca acuminata CaRAV. Subcellular localization and transcriptional self-activation experiments demonstrated that GeRAV1 was a nucleoprotein without self-activating activity. The GeRAV1 gene was constitutively expressed in the leaves, stems, and roots of the G. elegans, with the highest expression levels in roots. In addition, the expression of the GeRAV1 gene was rapidly up-regulated under abscisic acid (ABA), salicylic acid (SA), and methyl jasmonate (MeJA) stresses, suggesting that it may be involved in hormonal signaling pathways. Moreover, GeRAV1 conferred improved cold and sodium chloride tolerance in Escherichia coli Rosetta cells.ConclusionsThese findings provided a foundation for further understanding on the function and regulatory mechanism of the GeRAV1 gene in response to low-temperature stress in G. elegans.

Silicon nanoparticles and indole butyric acid positively regulate the growth performance of Freesia refracta by ameliorating oxidative stress under chromium toxicity.

Chromium (Cr) toxicity hampers ornamental crops' growth and post-harvest quality, especially in cut flower plants. Nano-enabled approaches have been developing with phenomenal potential towards improving floricultural crop production under heavy metal-stressed conditions. The current pot experiment aims to explore the ameliorative impact of silicon nanoparticles (Si-NPs; 10 mM) and indole butyric acid (IBA; 20 mM) against Cr stress (0.8 mM) in Freesia refracta. The results showed that Cr stress significantly reduced morphological traits, decreased roots-stems biomass, abridged chlorophyll (14.7%) and carotenoid contents (27.2%), limited gas exchange attributes (intercellular CO2 concentration (Ci) 24.8%, stomatal conductance (gs) 19.3% and photosynthetic rate (A) 28.8%), condensed proline (39.2%) and total protein (40%) contents and reduced vase life (15.3%) of freesia plants by increasing oxidative stress. Contrarily, antioxidant enzyme activities, MDA and H2O2 levels, and Cr concentrations in plant parts were remarkably enhanced in Cr-stressed plants than in the control. However, foliar supplementation of Si-NPs + IBA (combined form) to Cr-stressed plants increased defense mechanism and tolerance as revealed by improved vegetative and reproductive traits, increased biomass, photosynthetic pigments (chlorophyll 30.3%, carotenoid 57.2%) and gaseous exchange attributes (Ci 33.3%, gs 25.6%, A 31.1%), proline (54.5%), total protein (55.1%), and vase life (34.9%) of metal contaminated plants. Similarly, the improvement in the activities of peroxidase, catalase, and superoxide dismutase was recorded by 30.8%, 52.4%, and 60.8%, respectively, compared with Cr-stressed plants. Meanwhile, MDA (54.3%), H2O2 (32.7%) contents, and Cr levels in roots (43.3), in stems (44%), in leaves (52.8%), and in flowers (78.5%), were remarkably reduced due to combine application of Si-NPs + IBA as compared with Cr-stressed nontreated freesia plants. Thus, the hypothesis that the synergistic application of Si-NPs + IBA will be an effective approach in ameliorating Cr stress is authenticated from the results of this experiment. Furthermore, the study will be significant since it will demonstrate how Si-NPs and IBA can work synergistically to combat Cr toxicity, and even when added separately, they can improve growth characteristics both under stressed and un-stressed conditions.

Transcriptome analysis reveals how cadmium promotes root development and accumulates in Apocynum venetum, a promising plant for greening cadmium-contaminated soil

Cadmium (Cd) contamination poses a substantial threat the environment, necessitating effective remediation strategies. Phytoremediation emerges as a cost-efficient and eco-friendly approach for reducing Cd levels in the soil. In this study, the suitability of A. venetum for ameliorating Cd-contaminated soils was evaluated. Mild Cd stress promoted seedling and root growth, with the root being identified as the primary tissue for Cd accumulation. The Cd content of roots ranged from 0.35 to 0.55 mg/g under treatment with 10–50 µM CdCl2·2.5 H2O, and the bioaccumulation factor ranged from 28.78 to 84.43. Transcriptome sequencing revealed 20,292 unigenes, and 7507 nonredundant differentially expressed genes (DEGs) were identified across five comparison groups. DEGs belonging to the “MAPK signaling pathway-plant,” “monoterpenoid biosynthesis,” and “flavonoid biosynthesis pathway” exhibited higher expression levels in roots compared to stems and leaves. In addition, cytokinin-related DEGs, ROS scavenger genes, such as P450, glutathione-S-transferase (GST), and superoxide dismutase (SOD), and the cell wall biosynthesis-related genes, CSLG and D-GRL, were also upregulated in the root tissue, suggesting that Cd promotes root development. Conversely, certain ABC transporter genes, (e.g, NRAMP5), and some vacuolar iron transporters, predominantly expressed in the roots, displayed a strong correlation with Cd content, revealing the mechanism underlying the compartmentalized storage of Cd in the roots. KEGG enrichment analysis of DEGs showed that the pathways associated with the biosynthesis of flavonoids, lignin, and some terpenoids were significantly enriched in the roots under Cd stress, underscoring the pivotal role of these pathways in Cd detoxification. Our study suggests A. venetum as a potential Cd-contaminated phytoremediation plant and provides insights into the molecular-level mechanisms of root development promotion and accumulation mechanism in response to Cd stress.

Visualizing and quantifying 33P uptake and translocation by maize plants grown in soil.

Phosphorus (P) availability severely limits plant growth due to its immobility and inaccessibility in soils. Yet, visualization and measurements of P uptake from different root types or regions in soil are methodologically challenging. Here, we explored the potential of phosphor imaging combined with local injection of radioactive 33P to quantitatively visualize P uptake and translocation along roots of maize grown in soils. Rhizoboxes (20 × 40 × 1 cm) were filled with sandy field soil or quartz sand, with one maize plant per box. Soil compartments were created using a gravel layer to restrict P transfer. After 2 weeks, a compartment with the tip region of a seminal root was labeled with a NaH2 33PO4 solution containing 12 MBq of 33P. Phosphor imaging captured root P distribution at 45 min, 90 min, 135 min, 180 min, and 24 h post-labeling. After harvest, 33P levels in roots and shoots were quantified. 33P uptake exhibited a 50% increase in quartz sand compared to sandy soil, likely attributed to higher P adsorption to the sandy soil matrix than to quartz sand. Notably, only 60% of the absorbed 33P was translocated to the shoot, with the remaining 40% directed to growing root tips of lateral or seminal roots. Phosphor imaging unveiled a continuous rise in 33P signal in the labeled seminal root from immediate post-labeling until 24 h after labeling. The highest 33P activities were concentrated just above the labeled compartment, diminishing in locations farther away. Emerging laterals from the labeled root served as strong sinks for 33P, while a portion was also transported to other seminal roots. Our study quantitatively visualized 33P uptake and translocation dynamics, facilitating future investigations into diverse root regions/types and varying plant growth conditions. This improves our understanding of the significance of different P sources for plant nutrition and potentially enhances models of plant P uptake.

Comparative Evaluation of the Effect of Different Chelating Agents on Mineral Content and Erosion of Radicular Dentine: A FESEM-EDS Analysis.

Irrigating solutions play an important role in the debridement and disinfection of the root canal space, and thus, it is crucial to comprehend their effects on the composition and surface structure of radicular dentine. This study evaluated and compared the effects of 17% ethylenediaminetetraacetic acid (EDTA), 9% 1-hydroxyethylidene-1,1-bisphosphonate (HEBP) and 0.2% chitosan on the mineral content and erosion of radicular dentine when used as a final rinse. Sixty extracted human mandibular premolar teeth were decoronated and instrumented to ProTaper size F2. After final instrumentation, the samples were randomly divided into 4 groups (n=15) according to the type of final irrigant used: Normal saline (control), 17% EDTA, 9% HEBP and 0.2% chitosan. Field emission scanning electron microscopy was used to assess the erosion of radicular dentine, and energy dispersive X- ray spectroscopy was used to quantify the radicular dentine mineral composition at the coronal, middle and apical levels of all the prepared samples after final irrigation. The one-way analysis of variance was used for intra-group and inter-group comparisons of means, the Kruskal Wallis test for intra-group and inter-group comparisons of medians and Tukey's post hoc test for pairwise comparisons. There was no significant difference in the levels of calcium (Ca), phosphorus (P) and Ca/P ratio after final rinse with 17% EDTA, 9% HEBP and 0.2% chitosan at all three root levels (p>0.05); except at the coronal level, where 0.2% chitosan caused significantly less alteration in Ca levels and Ca/P ratio than 17% EDTA and 9% HEBP respectively (p<0.05). 17% EDTA, 9% HEBP and 0.2% chitosan caused no erosion at the middle and apical levels. Meanwhile, 17% EDTA and 9% HEBP caused moderate erosion at the coronal level. Alternatives to 17% EDTA during final irrigation can be 9% HEBP and 0.2% chitosan. (EEJ-2023-06-078).

Investigating the involvement of potato (Solanum tuberosum L.) StPHR1 gene in the combined stress response to phosphorus deficiency and aluminum toxicity.

Phosphorus deficiency and aluminum toxicity in acidic soils are important factors that limit crop yield. To further explore this issue, we identified 18 members of the StPHR gene family in the potato genome in this study. Through bioinformatics analysis, we found that the StPHR1 gene, an important member of this family, exhibited high expression levels in potato roots, particularly under conditions of phosphorus deficiency and aluminum toxicity stress. This suggested that the StPHR1 gene may play a crucial regulatory role in potato's resistance to phosphorus deficiency and aluminum toxicity. To validate this hypothesis, we conducted a series of experiments on the StPHR1 gene, including subcellular localization, GUS staining for tissue expression, heterologous overexpression, yeast two-hybrid hybridization, and bimolecular fluorescence complementation (BiFC). The results demonstrated that the StPHR1 gene is highly conserved in plants and is localized in the nucleus of potato cells. The heterologous overexpression of the gene in Arabidopsis plants resulted in a growth phenotype that exhibited resistance to both aluminum toxicity and phosphorus deficiency. Moreover, the heterologous overexpressing plants showed reduced aluminum content in the root system compared to the control group. Furthermore, we also identified an interaction between StPHR1 and StALMT6. These results highlight the potential application of regulating the expression of the StPHR1 gene in potato production to enhance its adaptation to the dual stress of phosphorus deficiency and high aluminum toxicity in acidic soils.

Accumulation of reactive carbonyl species in roots as the primary cause of salt stress‐induced growth retardation of Arabidopsis thaliana

AbstractSalt stress on plants induces an increase in reactive oxygen species (ROS), which then leads to the formation of reactive carbonyl species (RCS) such as acrolein and 4‐hydroxy‐(E)‐2‐nonenal (HNE), potent cytotoxins generated from lipid peroxides. We recently showed that salt‐stress treatment of Arabidopsis thaliana plants increased RCS levels, and exogenously added RCS‐scavenging chemicals alleviated the stress symptoms, indicating that RCS were responsible for the tissue damage in salt‐stressed plants. To obtain deeper insights into the role of RCS in stressed plants, we here analyzed changes in the levels of various RCS in the roots and shoots of A. thaliana. NaCl (90 mM) addition to the culture medium as a salt‐stress treatment caused growth inhibition and leaf chlorosis. Carbonyl analysis using HPLC revealed that the stress treatment induced a 2‐fold increase in the root levels of RCS, including acrolein, HNE and 4‐hydroxy‐(E)‐2‐hexenal (HHE). In the shoots, basal levels and stress‐induced increases of the RCS were lower than in roots. In the transgenic A. thaliana plants that overexpress the RCS‐scavenging enzyme 2‐alkenal reductase (AER) cDNA under the β‐estradiol (β‐ED)‐responsive promoter, salt stress induced less damage than in the wild‐type under β‐ED supplementation. The AER overexpression suppressed the stress‐induced increases in HNE, acrolein, HHE and (E)‐2‐hexenal in roots and in HNE in leaves, but not the ROS increase. These results suggest that the RCS increase in roots was the primary cause of salt‐induced damages. Enhancing RCS‐scavenging abilities, such as by AER overexpression, could be a new strategy to confer salt‐stress tolerance to plants.

Cleaning Ability of Gentlefile Rotary System Compared with Three Alternative Brands.

In this study, the cleaning ability of a stainless-steel rotary instrument called Gentlefile, was compared with three nickel-titanium rotary instruments. In this in vitro study, forty mandibular single-rooted premolars were randomly assorted into four groups: Gentlefile, ProTper Universal, RaCe files, and XP-Endo Finisher/ProTaper Universal system (n=10). Final instrumentation was done using the aforementioned files with 5.25% sodium hypochlorite and normal saline for root canal irrigation. Debris and smear layers were observed by the scanning electron microscope on the canal walls in the coronal, middle, and apical third of the root level, through a 4-point scoring system. The chi-square test and Kruskal-Wallis were used for data analysis. The Gentlefile demonstrated a promising outcome in smear layer clearance and debris removal compared with the other three rotary systems (P<0.05), specifically at the apical third of the root canal. Based on chi-square test results, there was a significant relationship between root canal cleaning (three levels of cleanliness) in ProTaper Universal (P=0.004) and Gentlefile (P=0.04) groups. Neither of the investigated systems achieved complete cleanliness. The Gentlefile rotary system can be capable of cleaning the apical third of root canals more than the other three groups including Protaper Universal, RaCe, and XP-Endo Finisher.

Evaluation of the infrazygomatic crest and mandibular buccal shelf in different sagittal skeletal patterns: a cone-beam computed tomography study

Abstract Objectives To compare bone thickness, bone height, and density in the infrazygomatic crest (IZC) and the mandibular buccal shelf (MBS) associated with different sagittal skeletal patterns. Material and Methods This retrospective study included three groups of which a Class I group consisted of 28 patients (24 female, 4 male, mean age: 26.46 ± 6.1), a Class II group comprised 31 patients (28 female, 3 male, mean age: 30.08 ± 5.9), and a Class III group included 26 patients (22 female, 4 male, mean age: 26.21 ± 5.3). Evaluations of bone characteristics were performed for both the IZC and MBS using cone-beam computed tomography (CBCT). Maxillary length and mandibular body length were also measured on lateral cephalograms. One-way ANOVA, chi-squared, and Spearman’s correlation tests were applied for statistical analysis. Results The Class II group showed significantly greater bone thickness at the distal root level of the maxillary second molar in IZC (p &lt; 0.05). While bone thickness showed no statistically significant difference in the MBS between the groups (p &gt; 0.05), the Class II group showed significantly higher bone height values at the distal root level of the mandibular second molar in the MBS (p &lt; 0.05). A statistically significant increase from the mesial aspect to the distal aspect was observed in total bone thickness in the Class I group and total bone thickness and height in the Class II and III groups in the MBS. The mandibular body length and SNB values were positively correlated with the total bone thickness and bone height of the MBS. Conclusions Bone thickness in the IZC and bone density in both the IZC and MBS were similar in the different sagittal skeletal patterns. Total bone thickness in the Class I and III groups and the total bone height in the Class II group showed a gradual increase from the mesial to the distal in the MBS. The length of the mandibular body may be a clinical clue to assist in the selection of the appropriate implantation area.

‘Self-Sustainability’ and ‘Performance’ in Microfinance – The Contextual Relevance of the Terms

The microfinance sector’s Paradigm Shift (PS) of the 1990s caused a drastic shift in the way Microfinance Organizations (MFOs) ensured their long-term survival and sustainability. The PS required MFOs to adopt a commercial approach in all their operations and pursue profitability and self-sustainability rather than depending on the provision of subsidized funds. Resorting to Schatzki’s (2002) ‘site of the social’ theoretical construct and DiMaggio and Powell’s (1983) institutional theory, this empirical paper aims to evaluate the real performance of Pakistan’s microfinance sector, following the PS, in terms of its strict adherence to its founding objectives of eradicating poverty and serving the extreme poor. This qualitative empirical research paper resorts to the Case Study approach to investigate the pre- and the post-Paradigm Shift eras of Pakistan’s microfinance sector for evaluating the sector’s real ‘success’ in accomplishing its founding aim of eradicating poverty at the grass root level. Analysis of the data reveals that the microfinance sector, in its pursuit of profitability and self-sustainability, following the PS, is compromising its founding commitment to its target beneficiaries – the extreme poor. The study contributes a theory, literature and empirical evidence-informed conceptual framework to help guide organizational change initiatives in the peculiar context of a social service/not-for-profit sector and predict outcome quality of such a change for all stakeholders. The study outcomes apprise policy-makers and practitioners on the possible negative consequences of the microfinance sector’s gradual transition from a ‘social-service’ to a ‘commercial’ model for the poor.

Assessing the Impacts and Challenges of Deployed Community Development Workers: The Case of Bishoftu Community Development Workers Training College Graduates

Community development is about placing individuals at the centre of the development process and helping them realize their potential. Community Development Workers (CDWs) are community-based resource persons who collaborate with other community activists to help fellow community members. The objective of this study is to assess factors that challenge the roles of CDWs. The study used both quantitative and qualitative study designs. Data were gathered through questionnaires, in-depth interviews and personal observation. The collected data was analyzed using mixed method of analysis of both quantitative and qualitative data. The study revealed that CDWs have proved to be key and important local actors and facilitators in bringing about attitudinal change among the communities they work with, are attracting the trust and confidence of the people they work with, have revitalized local social organizations as instruments of development, have tabled the agendas of the socially marginalized community groups, working on the principle of matching community inputs with resources, and their role approved to be well aligned with Development Policy as it fosters ‘community participation, promotes self-reliance, and is strategically fasten at the grass roots level. The study concludes that CDWs are facing challenges in contextualizing their role and institutional factors are discovered to be impeding the roles of CDWs. The study recommends a multi-disciplinary research involving educationalists, and social anthropologists could perhaps develop a more thorough of investigation and be more conclusive on the factors that influence CDWs performance initiated by this research.

The Development of an Informative Model for Namibian Women in ICT Careers

Information and communication technologies (ICTs) were identified by the United Nations as tools that enable and enhance economic development and sustainable development. Women are a social group that plays an important role in development from the family level through to community and national institutions. Despite their contribution in social and economic development, women happen to lack digital exposure and career guidance in challenging information technology careers. The challenge motivated the need to explore the occupational experience of Namibian women in information technology and factors influencing their information technology career growth. The study used qualitative research approach and a case study design focusing on Windhoek the capital city of Namibia. The sample size was 35 female workers from IT professions in Windhoek and 20 female students. The data were collected by means of questionnaires. The results showed that IT female’s workers and IT students are less represented in IT challenging jobs and IT challenging courses that include Artificial Intelligence (AI), Cybersecurity, Networking and Programming. Furthermore, the results showed that women in IT take fewer challenging positions such as computer technicians and system administrators due to the lack of skills and appropriate tools. Finally, IT female workers are being undermined due to the traditional belief that women are less efficient, hence, they do not rise to higher positions. Following the results, it was concluded that social and cultural factors affect women in the ICT profession and it is possible to develop the informative model for Namibia women in ICT through identification of challenges and developing strategies for overcoming the challenges. Following identified gaps, it is recommended stakeholders including the government and other institutions must maintain the involvements of the Namibian girl child in specialized ICT courses at grass root level from primary schools, through secondary schools up to tertiary institutions and avoid any form of gender segregation when enrolling ICT students.

Lumbosacral Plexogram: An Aid to Reconstructive Nerve Possibilities in the Lower Extremity.

The lumbosacral plexus is the network of nerves responsible for the motor and sensory function of the pelvis and lower limb. Our observation is that the anatomy of this plexus is less familiar to surgeons than that of the brachial plexus. Damage to the lumbosacral plexus and its terminal branches may have a significant impact on locomotion, posture, and stability. We have designed a visual representation of the lumbosacral plexus to aid clinicians treating peripheral nerve disorders. The utility is illustrated with a case report in which a patient underwent nerve transfers in the lower limb to restore function. A visual representation of the lumbosacral plexus is a valuable adjunct to a clinical examination and helps make sense of clinical signs. The color-coding of each root level and the arrangement of muscles from proximal to distal helps with visual recall. A clear assessment of complex lumbosacral plexus patients is essential for diagnosis and planning. As with the case described, a sound knowledge of the "plexogram" can identify solutions for complex patients and result in significant functional improvements. We hope it helps advance the field of nerve surgery and, particularly, nerve transfers.

Technology Empowerment of ASHA Workers Using E-Health Initiatives in Rural Rajasthan

Introduction: Electronic health (e-health) initiatives grant the tools and knowledge to improve health care, benefiting not only patients but also healthcare professionals and institutions. Objectives: To empower ASHA (Accredited Social Health Activist) technically in terms of using e-health initiatives and to assess feasibility and usefulness of such initiatives; to enhance digital health literacy and to observe the effect of training and skill development of grass root level healthcare workers engaged in primary care. Method: A prospective observational cohort study, in which ASHA’s were recruited in two selected villages of Rajasthan. They were trained to use a web-based application, developed for use on portable device (tablet) to screen, diagnose and provide health education on non-communicable diseases. In-depth interviews and group discussions were done with ASHAs to know their views about the tablet and application use. Results: There was 19.3%, 29.3% and 40% improvement in ASHAs’ ability to measure haemoglobin, blood sugar and blood pressure respectively and also an improvement of 86.5% in tablet and application operation was observed. This improvement in ability of ASHAs was highly significant for all above four parameters (p-value &lt;0.001). ASHAs agreed that the app was useful and helped them connect to the people and explain things more properly. They reported the beneficiaries understood more clearly through pop- up videos which also proved beneficial in increasing compliance. Conclusion: Training ASHA worker in e-health technology is feasible and can assist in screening and management of non-communicable diseases.

Genome-Wide Identification and Expression Analysis of Auxin Response Factor (ARF) Gene Family in Betula pendula

As the key transcription factors regulating auxin responsive genes expression, auxin response factors (ARFs) play critical roles in diverse aspects of plant growth and development. Betula pendula is a valuable ornamental tree, and the information on ARF gene family of B. pendula is needed for better understanding. The publication of the genome sequence of B. pendula enable to analyze the bioinformatics information and expression pattern of BpeARF gene family on the genome-wide basis. In this study, physical and chemical properties, chromosome location, phylogenetic relationship, gene structure, conserved domain, motif composition, and cis-acting element of BpeARF gene family were analyzed, and expression patterns of BpeARF genes were investigated using completely random design in different tissues and under exogenous NAA and drought treatments. A total of 17 BpeARF genes was identified from B. pendula genome, which were unevenly distributed on 13 chromosomes and encoded adequate proteins ranging from 613 to 1135 amino acids in length. Three BpeARF gene pairs were formed by segmental duplication, and the Ka/Ks values of these BpeARF gene pairs were less than 1. According to the phylogenetic relationship among B. pendula, Betula platyphylla, Populus trichocarpa, and Arabidopsis thaliana, the BpeARF genes were divided into four classes, and the intron/exon structure, conserved domain, and motif composition showed high similarity among the BpeARF genes within the same class. The cis-acting elements in the promoter regions of BpeARF genes were related to tissue development, hormone response, and stress resistance. Quantitative real-time PCR exhibited diverse expression patterns of BpeARF genes in different tissues and in response to exogenous auxin treatment and drought stress. The expressions of one, ten, seven, and three BpeARF genes were the high levels in buds, young leaves, stems, and roots, respectively. Under exogenous NAA treatment, six BpeARF genes in stems and roots were upregulated expression at all timepoints. Under drought stress, BpeARF7 and BpeARF15 were upregulated in stems and roots, and BpeARF5 and BpeARF6 were downregulated in leaves, stems, and roots. Our results provided valuable information for the classification and putative functions of BpeARF gene family, which may be helpful for selecting candidate genes and verifying gene function in the genetic engineering of birch trees in further research.

Kinetin-mediated reduction of cadmium accumulation in rice (Oryza sativa L.) via modulation of cell wall binding capacity in a NO-dependent manner

Cadmium (Cd) is a heavy metal that is toxic to both plant growth and human health. Kinetin, a precursor of cytokinin, plays crucial roles in the response of plants to abiotic stress. However, the mechanism underlying kinetin detoxification in Cd-induced stress remains poorly understood. In this study, we observed a significant increase in endogenous kinetin levels in both rice shoots and roots under Cd stress. Furthermore, the application of exogenous kinetin resulted in a reduction in hemicellulose content within cell walls, thereby reducing the cell wall's capacity to bind Cd and limit its entry into cells. In addition, exogenous kinetin downregulated the transcript levels of genes associated with Cd uptake and root-to-shoot translocation while simultaneously upregulating the transcript levels of genes involved in Cd efflux from cells and sequestration into vacuoles, consequently leading to reduced Cd accumulation in rice plants. Moreover, kinetin significantly decreased nitric oxide (NO) accumulation in response to Cd stress, potentially facilitating detoxification during Cd-induced stress through a NO-dependent mechanism. Overall, our findings suggest that kinetin enhances rice's tolerance to Cd by increasing the cell wall's ability to bind Cd and regulating gene expression related to Cd accumulation. These mechanisms may be NO-dependent and could potentially inform the development of new strategies for mitigating Cd toxicity in agricultural systems.