To evaluate the effects of LLL on IL-6 levels in the GCF and the Probing Pocket Depth measurements (PPD) during orthodontic tooth movement in periodontally affected rabbits. Twenty-four rabbits were divided into 3 groups: Group 1(G1), healthy rabbits with orthodontic movement, Group 2(G2) periodontally affected rabbits and orthodontic movement, Group 3(G3) periodontally affected rabbits with orthodontic movement and LLL therapy. A 0.014 stainless steel spring was inserted in the upper central incisors to produce 60gm force. Laser CAT 500 was applied for 3min/day for 2 weeks. PPD measurements were obtained at base line and after 14 days with electronic periodontal probe. IL-6 levels increased gradually after application of orthodontic force, afterwards the 8th day, a significant difference in the Il-6 levels between G1 vs. G2 and G2 vs. G3 was observed. PPD measurements showed significant difference between the three groups at base line and after 14 days. LLL application can enhance periodontal ligament regeneration and decrease the periodontal tissue destruction through suppression of IL-6 levels.