Level Density Research Articles

Quantitative cytoarchitectural phenotyping of deparaffinized human brain tissues.

Advanced 3D imaging techniques and image segmentation and classification methods can profoundly transform biomedical research by offering deep insights into the cytoarchitecture of the human brain in relation to pathological conditions. Here, we propose a comprehensive pipeline for performing 3D imaging and automated quantitative cellular phenotyping on Formalin-Fixed Paraffin-Embedded (FFPE) human brain specimens, a valuable yet underutilized resource. We exploited the versatility of our method by applying it to different human specimens from both adult and pediatric, normal and abnormal brain regions. Quantitative data on neuronal volume, ellipticity, local density, and spatial clustering level were obtained from a machine learning-based analysis of the 3D cytoarchitectural organization of cells identified by different molecular markers in two subjects with malformations of cortical development (MCD). This approach will grant access to a wide range of physiological and pathological paraffin-embedded clinical specimens, allowing for volumetric imaging and quantitative analysis of human brain samples at cellular resolution. Possible genotype-phenotype correlations can be unveiled, providing new insights into the pathogenesis of various brain diseases and enlarging treatment opportunities.

Greenness Exposure and Mortality Risk in a Cardio-Oncologic Population.

Knowledge is lacking on the relationship between greenness and mortality in cancer survivors who experience coronary artery disease (CAD), a cardio-oncologic population. We aimed to investigate the association between residential greenness exposure and all-cause mortality in a cardio-oncologic population. Cancer survivors undergoing percutaneous coronary intervention at the Rabin Medical Center in Israel between 2004 and 2014 were included in the study. Clinical data were collected from medical records during index hospitalization and from the Israeli National Cancer Registry. Residential greenness was estimated by the normalized difference vegetation index (NDVI), a satellite-based index derived from Landsat imagery at a 30-meter spatial resolution, with larger values indicating higher levels of vegetative density (ranging between -1 to 1). Mortality follow-up data were obtained through the end of 2021. Cox models were used to assess the hazard ratios (HRs) for all-cause mortality per 1SD increase in NDVI. Among 1,331 patients analyzed [mean (SD) age, 75.6 (10.2) years, 373 (28%) females], the mean (SD) NDVI within a 300-meter radius was 0.12 (0.03). During a median follow-up period of 12.0 (IQR 9.2-14.7) years, 883 (66%) participants died. After adjustment for potential confounding factors, including residential socioeconomic status, air pollution, and smoking, NDVI was inversely associated with mortality hazard [HR (95% CI) = 0.93 (0.86, 0.99); p=.042]. The association was stronger among individuals with more recently (<10 years) diagnosed cancer [HR (95% CI) = 0.89 (0.81, 0.98); p=.019]. In a cohort of cardio-oncologic patients, greenness was independently associated with lower mortality.

Overexpression of StERECTA enhances drought tolerance in Arabidopsis thaliana

Drought is a major abiotic stresses that severely hinder plant growth and agricultural productivity. The receptor-like kinase gene, ERECTA, has been proved to play important role in promoting the response to abiotic stress in crops. However, the specific molecular mechanisms underlying the drought resistance mediated by ERECTA in potato (Solanum tuberosum L.) are not well understood. In this study, sequence analysis confirmed that the StERECTA gene contains eight leucine-rich repeat (LRR) domains and an S_TKc domain, and these domains were highly conserved in Solanaceae family. Under drought stress, Arabidopsis thaliana strains overexpressing StERECTA showed increased biomass, proline (PRO) content, and antioxidant enzyme activities compared to the wild-type strains while the mutant ERECTA strain (er105) exhibited opposite phenotype. Additionally, StERECTA overexpression upregulated the expression of drought response marker genes (LEA3, DREB2A and P5CS1), improved levels of ABA and auxin, reduced stomatal density and relative expression level of stomatal development related genes (SPCH, FAMA and MUTE). Furthermore, Co-immunoprecipitation (Co-IP) assays demonstrated that StERECTA physically interacted with the YODA protein. In conclusion, our study provides new insights into the role and regulatory mechanism of StERECTA in response to drought stress. These findings may serve as a basis for genetic improvement of potato to enhance their tolerance to abiotic stress.

The role of wild red deer on soil physical properties in a Mediterranean ecosystem: insights from a Portuguese mountain.

In this study we aimed to assess the role of wild red deer, along with other ungulates such as roe deer and wild boar, in the soil's physical properties, namely soil penetration resistance and depth (used as a proxy for soil compaction), hydraulic conductivity (a proxy for water infiltration), and the proportion of soil stable aggregates. Results showed that, at the density level found in our study area, red deer have a neutral effect at the soil level, not causing significant soil compaction or significantly influencing measured soil functions.

Fishery Management Enforcement Gradients to Achieve Fishery Goals

Marine protected areas (MPAs) can allow some fish populations to rebuild within their borders in areas impacted by overfishing, but the effectiveness of reserves is highly dependent on how effectively fishing mortality is controlled, which in turn depends on the level of fishery management implementation. In Cuba’s Gardens of the Queen MPA, the largest in the Caribbean, a variety of fishery management measures have been implemented to ensure the social, economic, and political viability of protecting such a large area. Here, we evaluate the biological response, in terms of fish density and the biomass of commercially valuable and ecologically important reef fish species, to a spatial gradient of fishery management enforcement, in terms of fish density and biomass, of commercially valuable and ecologically important reef fish species. The enforcement gradient is characterized by the level of protection, fishing effort, patrolling effort, distance to the nearest fishing port, and fishing intensity. Fish density and biomass were estimated from visual scuba surveys. Areas with higher levels of enforcement support higher levels of average biomass (up to 1378 kg/ha) and density (up to 2367 indv./ha) of commercially important fishes in comparison to areas with very low or no enforcement (estimates of 757 kg/ha average biomass and 1090 indv./ha average density, respectively). These fish density and biomass levels can serve as proxies in the development of harvest control rules that adjust fishing pressure according to the ratio of fished density or biomass to unfished density or biomass, through the use of the MPA Density Ratio method.

Current status of white mango scale (Aulacaspis Tubercularis Newstead) and associated factors in Assosa district western Ethiopia

White mango scale (Aulacaspis tubercularis Newstead; Hemiptera: Diaspididae) is a major insect pest of mango production threatening production and marketing of mango in Ethiopia. The present study was conducted to determine the current status of the white mango scale (WMS) and to identify the factors contributing to the damage level of the pest. A cross-sectional survey was conducted in ten selected kebeles in Assosa district, western Ethiopia, during 2021/2022 cropping season. Data on infestation level and WMS life-stage abundance were recorded from fifty randomly selected mango orchards. Data on the prevalence of WMS and factors influencing the distribution and damage levels of white mango scale infestations were analyzed using SAS and SPSS software respectively. The results of this study indicate that WMS infestation was prevalent in all assessed mango growing areas of the district. There was a significant (p < 0.05) difference between kebeles and mango orchards in infestation level and WMS life-stage population densities. Poor cultivation, crop husbandry practices, and pest management strategies have been identified as the major contributing factors for high pest infestation. The study indicates that WMS infestations are increasing in the study area; thus, there has to be strong efforts towards the implementation of safe and effective management strategies.

Bloch-Wave Phase Matching of High Harmonic Generation in Solids.

There has been a longstanding doubt that the conversion efficiency of high harmonics in solids is much lower than expected at such a high level of electron density. To address this issue, we revisit the dynamical process of high harmonic generation (HHG) in solids in terms of wavelet interference. We find that the constructive interference among the wavelets has intrinsic consistency with the phase matching of coupled waves in nonlinear optics, which we call Bloch-wave phase matching. Our analysis indicates that most of the wavelets are out of phase and coherently canceled out during the solid HHG process, leading to only a small fraction of excited electrons effectively contributing to HHG. Consequently, the conversion from the excited electron to HHG is fairly low. Moreover, a Bloch-wave phase-matching scheme is proposed and a nearly 3 orders of magnitude enhancement of solid HHG can be achieved by engineering the crystal structures. Our Letter addresses a longstanding doubt and provides a novel idea and theoretical guidance for realizing efficient all-solid-state tabletop ultraviolet light sources.

Laboratory Evaluation of Geosynthetic Interface Friction under Low Stress.

In landfill cover, geosynthetic packages are often used to fulfil different and simultaneous functions: drainage, waterproofing, separation, reinforcement, and soil protection. In this regard, various types of geosynthetics are combined in succession to allow for water and biogas drainage and to waterproof, reinforce, and provide protection from erosion over the useful lifetime, ranging over many decades if we consider the long phases of disposal, closure, and quiescence of the landfill itself. The creation of the composite cover barrier requires the evaluation of various interfaces' frictional strength under low contact stresses, both in static and seismic cases. The main purpose of this study is to summarize the results of past laboratory tests carried out on different geosynthetic-geosynthetic and geosynthetic-soil-geosynthetic interfaces using experimental instrumentation developed at the geotechnical laboratory of the University of Padua, which allows for the characterization of the interface geosynthetic friction at low contact stresses. The main aspects highlighted are the kinematic mode of failure, the wearing of the contact surfaces, the presence or absence of interstitial fluid, and, finally, the density level of the granular soil in contact with the geosynthetics.

Transformable 3D curved high-density liquid metal coils – an integrated unit for general soft actuation, sensing and communication

Rigid solenoid coils have long been indispensable in modern intelligent devices. However, their sparse structure and challenging preparation of flexible coils for soft robots impose limitations. Here, a transformable 3D curved high-density liquid metal coil (HD-LMC) is introduced that surpasses the structural density level of enameled wire. The fabrication technique employed for high-density channels in elastomers is universally applicable. Such HD-LMCs demonstrated excellent performance in pressure, temperature, non-contact distance sensors, and near-field communication. Soft electromagnetic actuators thus achieved significantly improved the electromagnetic force and power density. Moreover, precise control of swinging tail motion enables a bionic pufferfish to swim. Finally, HD-LMC is further utilized to successfully implement a soft rotary robot with integrated sensing and actuation capabilities. This groundbreaking research provides a theoretical and experimental basis for expanding the applications of liquid metal-based multi-dimensional complex flexible electronics and is expected to be widely used in liquid metal-integrated robotic systems.

The Effectiveness of Artificial Intelligence-based Pedicle Screw Trajectory Planning in Patients With Different Levels of Bone Mineral Density.

Retrospective cohort study. To evaluate the effectiveness of pedicle screw trajectory planning based on artificial intelligence (AI) software in patients with different levels of bone mineral density (BMD). AI-based pedicle screw trajectory planning has potential to improve pullout force (POF) of screws. However, there is currently no literature investigating the efficacy of AI-based pedicle screw trajectory planning in patients with different levels of BMD. The patients were divided into 5 groups (group A-E) according to their BMD. The AI software utilizes lumbar spine CT data to perform screw trajectory planning and simulate AO screw trajectories for bilateral L3-5 vertebral bodies. Both screw trajectories were subdivided into unicortical and bicortical modes. The AI software automatically calculating the POF and pullout risk of every screw trajectory. The POF and risk of screw pullout for AI-planned screw trajectories and AO standard trajectories were compared and analyzed. Forty-three patients were included. For the screw sizes, AI-planned screws were greater in diameter and length than those of AO screws (P<0.05). In groups B-E, the AI unicortical trajectories had a POF of over 200N higher than that of AO unicortical trajectories. POF was higher in all groups for the AI bicortical screw trajectories compared with the AO bicortical screw trajectories (P<0.05). AI unicortical trajectories in groups B-E had a lower risk of screw pullout compared with that of AO unicortical trajectories (P<0.05). AI unicortical screw trajectory planning for lumbar surgery in patients with BMD of 40-120mg/cm3 can significantly improve screw POF and reduce the risk of screw pullout.

Astrophysical phenomena explainable by the particles’ density levels in a Cold Genesis Theory

The paper shows that some astrophysical phenomena such as the initial TOV limit of neutron stars’ mass and the transition density interval from neutron star to a quark star, can be explained unitary, by the specific structure and the density levels of the fermionic elementary particles specific to the particle models of a Cold genesis theory of the author: superdense centroid, kerneloid and photonic shell maintained by etherono-quantonic vortex/vortices of magnetic moment(s), which explain and physical phenomena such as: the connection between the photon’s structure and the electronic neutrinos, the scattering centers experimentally evidenced inside the proton at electron-proton scattering at high and very high energies and inside the electron by X-rays, the Compton effect, the nuclear and the strong force, in a fractalic scenario of particles’ forming, from the considered etherono-quantonic energy.

The heat-mortality association in Jordan: Effect modification by greenness, population density and urbanization level

BackgroundThe Middle East is one of the most vulnerable regions to the impacts of climate change, yet evidence of the heat-related mortality remains limited in this area. Our present study investigated the heat-mortality association in Jordan and the potential modifying effect of greenness, population density and urbanization level on the association. MethodsFor each of the 42 included districts, daily meteorological and mortality data from 2000 to 2020 were obtained for the warmest months (May to September). First, a distributed lag non-linear model was applied to estimate the district level heat-mortality association, then the district specific estimates were pooled using multivariate meta-regression models to obtain an overall estimate. Last, the modifying effect of district level greenness, population density and urbanization level was examined through subgroup analysis. ResultsWhen compared to the minimum mortality temperature (MMT, percentile 0th, 22.20 °C), the 99th temperature percentile exhibited a relative risk (RR) of 1.34 (95 % CI 1.23, 1.45). Districts with low greenness had a higher heat-mortality risk (RR 1.39, 95 % CI 1.22, 1.58) when compared to the high greenness (RR 1.28, 95 % CI 1.13, 1.45). While heat-mortality risk did not significantly differ between population density subgroups, highly urbanized districts had a greater heat-mortality risk (RR 1.41, 95 % CI 1.23, 1.62) as compared to ones with low levels of urbanization (RR 1.32, 95 % CI 1.13, 1.55). Districts with high urbanization level had the highest heat-mortality risk if they were further categorized as having low greenness (RR 1.63, 95 % CI 1.30, 2.04). ConclusionExposure to heat was associated with increased mortality risk in Jordan. This risk was higher in districts with low greenness and high urbanization level. As climate change-related heat mortality will be on the rise, early warning systems in highly vulnerable communities in Jordan are required and greening initiatives should be pursued.

Carbon and Nitrogen Stocks in Soil under Native Pastures in the Pantanal Wetland Biome, Brazil

The Pantanal has a high diversity of native pastures that provide food for many wild and domestic animals. Pantanal cattle raising is practiced in an extensive grazing-based system that varies according to the flood levels in the area. This study aimed to evaluate the fractions of soil organic matter in areas of native pastures under different uses and to quantify C and N stocks in sandy soils of the Pantanal. Soil samples from three native pastures differentiated by the predominance of Hymenachne amplexicaulis, Axonopus purpusii, and Mesosetum chaseae under different land use systems (continuous grazing and no grazing for five years) were collected and used to quantify the contents of carbon, nitrogen, and humic fractions. The dynamics of SOM are modified in grazed areas of the Pantanal, with influence on C and N, including their stocks. Native pastures of Axonopus purpusii and Hymenachne amplexicaulis showed an increase in organic matter after five years without grazing, while Mesosetum chaseae showed lower soil density and nitrogen levels. The highest C stock was observed in ungrazed areas of H. amplexicaulis (127.41 Mg ha−1 in the 0–40 cm layer). The dynamics of nitrogen in Pantanal pastures are influenced by the type of vegetation and land management, with higher nitrogen content in the surface layer (0–10 cm) and an increasing C/N ratio with soil depth, indicating lower nitrogen availability.

Effect of varying levels of plant density on growth of Yardlong bean

Effect of varying levels of plant density on growth of Yardlong bean

Losses and destabilization of soil organic carbon stocks in coastal wetlands converted into aquaculture ponds.

Coastal-wetlands play a crucial role as carbon (C) reservoirs on Earth due to their C pool composition and functional sink, making them significant for mitigating global climate change. However, due to the development and utilization of wetland resources, many wetlands have been transformed into other land-use types. The current study focuses on the alterations in soil organic-C (SOC) in coastal-wetlands following reclamation into aquaculture ponds. We conducted sampling at 11 different coastal-wetlands along the tropical to temperate regions of the China coast. Each site included two community types, one with solely native species (Suaeda salsa, Phragmites australis and Mangroves) and the other with an adjacent reclaimed aquaculture pond. Across these 11 locations we compared SOC stock, active OC fractions, and soil physicochemical properties between coastal wetlands and aquaculture ponds. We observed that different soil uses, sampling sites, and their interaction had significant effects on SOC and its stock (p < .05). Reclamation significantly declined SOC concentration at depths of 0-15 cm and 15-30 cm by 35.5% and 30.3%, respectively, and also decreased SOC stock at 0-15 cm and 15-30 cm depths by 29.1% and 37.9%, respectively. Similar trends were evident for SOC stock, labile organic-C, dissolved organic-C and microbial biomass organic-C concentrations (p < .05), indicating soil C-destabilization and losses from soil following conversion. Soils in aquaculture ponds exhibited higher bulk density (BD; 11.3%) and lower levels of salinity (61.0%), soil water content (SWC; 11.7%), total nitrogen (TN) concentration (23.8%) and available-nitrogen concentration (37.7%; p < .05) than coastal-wetlands. Redundancy-analysis revealed that pH, BD and TN concentration were the key variables most linked with temporal variations in SOC fractions and stock between two land use types. This study provides a theoretical basis for the rational utilization and management of wetland resources, the achievement of an environment-friendly society, and the preservation of multiple service functions within wetland ecosystems.

Impact of planting density and shoot thinning on alstroemeria flowering, soil attributes and cost economics

A field experiment was conducted to assess the impact of various planting densities and levels of shoot thinning on cut flower yield, quality, soil chemical properties and cost economics in the commercially cultivated alstroemeria cultivar 'Capri'. The experiment involved three planting densities (4, 6 and 8 plants/m2) along with three shoot thinning levels (10 %, 20 % and 30 %). It was observed that higher planting density (4 plants/m2) promoted the length of cut stem, early bud formation, and flowering, optimal quality parameters, including maximum stem thickness, cut stem weight, floret quantity/stem, floret size, number of flowering stems/plant (yield) and vase life. Exclusive use of higher vegetative mass removal (30 % shoot thinning) was found to improve quality parameters such as the length of cut stem, number of flowering stems, stem thickness, weight of cut stem, floret quantity/stem, floret size, vase life, and early flowering. Soil samples were collected from each treatment and the consequent lab analysis revealed that the lower planting density and shoot thinning level resulted in maximum soil organic carbon, nitrogen, phosphorous and potassium content. In conclusion, planting density of 4 plants/m2 and 30 % shoot thinning emerged as the optimal combination for enhancing quantitative parameters in commercial alstroemeria cultivation. The study underscores the importance of strategic planting practices and vegetative mass management for maximizing yield and quality in alstroemeria production, along with ensuring higher economic returns.

Effect of stocking density and feeding regimes on physiological recovery in stunted Labeo rohita fingerlings reared in cages

Aim: To find out the physiological recovery status of stunted fingerling of Labeo rohita reared in floating net cages at different stocking densities and feeding rations in cages. Methodology: Stunted fingerling (8 months) of L. rohita with an average body weight of 38.23 ± 1.90 g and length 14.65 ± 0.30 cm were stocked in cages at different densities viz. T1 (10), T2 (15), T3 (20) and T4 (25) fish m-3 and varying levels of feeding rations such as 3, 4, 5 and 6% for 330 days with crude protein (28%) pellet were fed twice a day. The 4×4 factorial design was used, and each stocking density and feeding level were triplicated. A total of 48 numbers of square shaped floating net cage, made of HDPE, each measuring 27 m-3 with 6 mm mesh size were used for the experiment. Results: A significant physiological recovery was observed in the T1 (10*5 and 10*6) treatment groups, which showed highest Hb (11.23± 0.08 g d l-1), RBC (2.83 ± 0.03 ×106 cum m-1), PCV (45.76 ± 0.16 %) and lymphocyte (69.32 ± 0.12 %) content at the end of the experiment. Similarly, significant changes were observed in glucose, cortisol, and lactate dehydrogenase, which were highest in T4 and normal in T1 group as the experiment progressed. Interpretation: The result of present study recommends that cage culture of stunted Labeo rohita is possible in Indian reservoirs with lower stocking density 10 m-2 and feeding ration of 5% body weight which maintains better physiological conditions for optimal growth of fish. Key words: Cage culture, Feeding ration, Labeo rohita, Physiological parameters, Stunted, Stocking density

The effect of complex formation on the static and frequency-dependent nonlinear optical properties: A combined experimental and theoretical investigation

A novel mixed ligand Mn(II) complex of 6-Bromopyridine-2-carboxylic acid (6Brpca) and 4,4′-dimethyl-2,2′-dipyridyl has been prepared and structurally characterized using single-crystal X-ray diffraction. The spectroscopic properties were also analyzed by using FT-IR and UV–Vis spectral techniques. The coordination complexes having transition metal ions are known to have promising optical nonlinearity behavior. Therefore, B3LYP level density functional theory was used to investigate first- and second-order hyperpolarizabilities (β and γ) and provide a deep understanding of the relation between the structure and NLO properties. The calculations of frequency-dependent α, β, and γ at frequencies of ω = 0.0856252 and 0.0428126 au. for 6Brpca and Dmdpy ligands as well as Mn(II) complex have been also carried out using B3LYP/LanL2DZ level. Especially second harmonic generation (SHG) first and second hyperpolarizabilities (β(−2ω;ω,ω) and γ (−2ω;ω,ω,0)) parameters for Mn(II) complex have been calculated as 11448 × 10−30 and 680035 × 10−36 esu, respectively. It has been determined that there is a tremendous increase in β and γ parameters when 6Brpca and Dmdpy ligands coordinate to the high spin multiplicity Mn(II) ion. Theoretical calculations revealed that the large first- and second-order hyperpolarizabilities are caused by strong intramolecular charge transfer between the transition metal and the coordinated ligands. These results indicate that the the organometallic complex under investigation is valuable candidate for optoelectronic and photonic applications.

Effect of Stocking Density on the Growth of the Endangered Amazonian Fish Hypancistrus zebra (Siluriformes: Loricariidae)

ABSTRACTStocking density has a significant influence on animal homeostasis, and so is a crucial factor in the management of organisms in captivity and an important role in ex situ conservation of endangered species. Hypancistrus zebra is an endemic fish species from the Xingu River, Brazil—considered Critically Endangered since the early 2000s due to fishing pressure and, more recently, due to habitat changes resulting from the construction of the Belo Monte Hydroelectric Dam. The implementation of appropriate management in captivity is of utmost importance for its conservation, particularly in enhancing ex situ breeding techniques. This study aimed to evaluate the growth of juvenile H. zebra in aquaria with three different stocking density levels. The juveniles were distributed between three treatments, each with three replicates. We found no correlation between biomass and aquarium volume. The highest correlation coefficients were found between growth rates and densities related to the number of individuals per shelter area. Mortality rate was directly related to the initial number of fish in the aquariums, with larger populations showing higher mortality rates. The treatment with lower stocking density displayed the highest growth rate, while the treatment with higher density had the lowest growth rate, with a 23.38% difference between them.

The influence of different stocking density on growth rate and survival rate koi larvae (Cyprinus carpio)

Abstract Stocking density has an important role in the survival and growth of fish larvae. The purpose of this study was to determine the optimal stocking density level for larval rearing with improved feed. Tubifex sp. which has a high protein content of about 57% and fat as much as 13%. The method used was the experimental method with a completely randomized design (CRD) on 4 different treatments of stocking density with 3 replications. The treatments given were P0/Control (1 individual/L), P1 (2 individuals/L), P2 (3 individuals/L), and P3 (4 individuals/L) maintained for 49 days. The results obtained during the study were the growth rate values of P0 (0.021 gram/day), P1 (0.017 gram/day), P2 (0.016 gram/day), and P3 (0.017 gram/day). The survival rates are P0 (90.00%), P1 (91.00%), P2 (90.33%), and P3 (87.50%). Absolute length values P0 (1.94cm ), P1 (1.70cm ), P2 (1.70cm), and P3 (1.77 cm) The quality of the water obtained is that the temperature ranges from 26–29, the pH is 6.8–8.1, and the DO is 5–6. The results showed that different stocking densities had no significant effect (p &gt; 0.05) on the survival and growth rate of koi fish.