20-Hydroxyecdysone, 24(28)-dehydromakisterone A, makisterone C, polypodine B, ajugasterone C, together with the two new phytoecdysteroids carthamosterone and 5-deoxykaladasterone, were isolated from the roots of Leuzea carthamoides. The herb yielded 20-hydroxyecdysone, 24(28)-dehydromakisterone A, makisterone C and carthamosterone.