Lesion Sterilization And Tissue Repair Research Articles

The application of lesion sterilization and tissue repair 3Mix-MP for treating rat's dental pulp tissue

Background: Lesion sterilization and tissue repair (LSTR) 3Mix-MP are three broad-spectrum antibiotics, including metronidazole, ciprofloxacin and minocycline are mixed with propylene glycol or macrogol. There is the possibility ofthe healing process that marked proliferation ofnew blood vessels and proliferation offibroblasts in the treatment ofirreversible pulpitis by pulp capping LSTR 3MixMP because of the principle of the method LSTR 3Mix-MP is to kill bacteria. Purpose: The purpose of this study to prove the effect of LSTR 3Mix-MP on chronic inflammation and the healing process in rat dental pulp tissue in vivo. Methods: Rattus norvegicus anaesthetized by using ketamine and xylazine dissolved in sterile isotonic saline solution (0.2 ml/50gr mm) on the upper right thigh. Cavity preparation class I to perforation by using a low speed tapered diamond round bur. In the treatment group, rats were treated 3Mix-MP at a dose of10 mg and then covered with glass ionomer cement for 7 days on the pulp that has been opened for 3 days. The control group treated with saline irrigation on the pulp that has been opened for 3 days. Rats were killed after seven days, and then made preparations pulp tissue to count the number oflymphocytes, macrophages, plasma cells, blood vessels, and fibroblasts Results: There is an increase in the average number ofmacrophage cells, plasma, and fibroblasts; and decreased lymphocytes and blood vessels in the treated group exposure LSTR 3Mix-MP. Conclusion: LSTR 3Mix-MP can reduce chronic inflammation process and enhance the healing process in rat dental pulp tissue.

Nonsurgical Root Canal Therapy of Large Cystic Periapical Lesions Using Simple Aspiration and LSTR (Lesion Sterilization and Tissue Repair) Technique: Case Reports and Review

Introduction: This review and case report presents the treatment of two large cystic periapical lesions using simple aspiration technique for decompression and triple antibiotic paste for disinfection of the root canal system. Methods: A non-surgical approach was followed for the two large cystic periapical lesions. Simple aspiration irrigation technique along with lesion sterilization therapy with triple antibiotic paste was used without any surgical intervention. Results: Complete periapical healing of both the cyst like periapical lesions occurred without any surgical intervention. A 1 year follow-up radiograph showed complete healing of the lesion. Conclusion: Pathogenesis, histology, and molecular cell biology of inflammatory apical true cysts are not different from pocket cysts. Therefore, inflammatory apical true cysts might regress after nonsurgical root canal therapy by the mechanism of apoptosis or programmed cell death, similar to inflammatory pocket cysts. Non-surgical approach using a combination of Simple Aspiration technique and LSTR (lesion sterilization and tissue repair) technique was successful in healing large cyst-like periradicular lesions.

Lesion Sterilization and Tissue Repair (LSTR): A Review

Lesion Sterilization and Tissue Repair (LSTR): A Review

Lesion Sterilization and Tissue Repair (LSTR)-3mix MP Therapy showed Reliable Efficacy against the Most Resistant Endodontic Bacteria Enterococcus faecalis

Sterilization of bacteria in root canal system is one of the prominent problems. Some bacteria could remain in root canal even after using conventional medicaments. Evidences suggested that Enterococcus faecalis (E. faecalis) caused substantial root canal infections. So, elimination of such organism is important to achieve treatment success. Research hypothesis: Sterilization of root canal with Lesion Sterilization and Tissue Repair (LSTR)-3mix MP Therapy is thought to be more effective against E. faecalis comparing to single antibiotic. Methods: In-vitro cross-sectional with interventional type of study to observe the zone of inhibitions by E. faecalis culture using LSTR- 3mix (metronidazole, ciprofloxacin and minocycline) and calcium hydroxide (control). Results: The results strongly recommended that LSTR-3mix sufficiently able to inhibit E. faecalis growth. DOI: http://dx.doi.org/10.3329/cdcj.v9i2.12311 City Dental College J. Volume-9, Number-2, July-2012

A Comparative Evaluation of Noninstrumentation Endodontic Techniques with Conventional ZOE Pulpectomy in Deciduous Molars: An in vivo Study

ABSTRACT Pulp exposures secondary to caries are the most common in primary teeth due to the relatively large size of pulp chamber. Pulp in primary teeth is capable of healing, following control of infection and inflammation. Pulpotec and LSTR are the simple noninstrumentation endodontic treatment procedures which are capable of compensating for the inconvenience caused by conventional pulpectomy, and thereby preserve the vitality of the pulp. This study was conducted to assess clinical efficacy of pulpotec and LSTR and compare with that of conventional ZOE pulpectomy at 1, 3, 6 and 12 months postoperatively. Around 34 children in the age group of 4 to 9 years with deep carious lesions affecting the pulps of 60 primary mandibular molars were randomly divided into three groups with 20 teeth in each group. Results Clinical evaluation was done at 1 month's interval. Both clinical and radiographic evaluations were done at 3, 6 and 12 months. Data obtained was analyzed statistically using Fisher's exact test. The results concluded that pulpotomy and pulpotec could be a good alternative for conventional ZOE pulpectomy. Long-term radiographic evaluations should be undertaken to further strengthen the efficacy of lesion sterilization and tissue repair (LSTR) as NIET.

Susceptibility of Enterococcus faecalis to a Combination of Antibacterial Drugs (3Mix) in vitro

It has been reported that enterococci cause significantly persistent root canal infection, especially after Ca(OH)2 intracanal dressing, and that they often show tolerance to certain antibacterial drugs. We aimed to evaluate the susceptibility of enterococci to a combination of antibacterial drugs, i.e. ciprofloxacin, metronidazole and minocycline (3Mix), which is used for Lesion Sterilization and Tissue Repair (LSTR) therapy. The minimum inhibitory concentrations (MICs) of ciprofloxacin and minocycline on E. faecalis (6 strains) and E. faecium (1 strain) were 5–20 μg/mL, respectively, and no inhibitory effect was observed with metronidazole. However, 3Mix (100 μg each/mL), as a mixture, inhibited the growth of every strain completely. In addition, 3Mix also inhibited all bacterial growth in faeces (16 samples). The present result strongly indicates that 3Mix is sufficiently able to inhibit enterococcal growth, and may be useful for endodontic treatment, even in cases where enterococci are suspected to cause endodontic disorders.