Lepidoptera Research Articles

PGE2 Binding Affinity of Hemocyte Membrane Preparations of Manduca sexta and Identification of the Receptor-Associated G Proteins in Two Lepidopteran Species.

Prostaglandin E2 (PGE2) is an eicosanoid that mediates a range of physiological actions in vertebrates and invertebrates, including reproduction and immunity. The PGE2 receptor was identified and functionally assessed in two lepidopteran insects, Manduca sexta and Spodoptera exigua. However, its binding affinity to the receptor has not been reported. The PGE2 receptor is a G-protein coupled receptor (GPCR) although its corresponding G-protein is not identified. PGE2 binding assays were performed with membrane preparations from hemocytes of M. sexta larvae. We recorded an optimal binding in 4 h reactions conducted at pH 7.5 with 12 nM tritium-labeled PGE2. We found that hemocytes express a single population of PGE2 binding sites with a high affinity (Kd = 35 pmol/mg protein), which are specific and saturable. The outcomes of experiments on the influence of purine nucleotides suggested these are functional GPCRs. A bioinformatics analysis led to a proposed trimeric G-protein in the S. exigua transcriptome, in which the Gα subunit is classified into five different types: Gα(o), Gα(q), Gα(s), Gα(12), and Gα(f). After confirming expressions of these five types in S. exigua, individual RNA interference (RNAi) treatments were applied to the larvae using gene-specific double-stranded RNAs. RNAi treatments specific to Gα(s) or Gα(12) gene expression significantly suppressed the cellular immune responses although the RNAi treatments specific to other three Gα components did not. While PGE2 treatments led to elevated hemocyte cAMP or Ca2+ levels, the RNAi treatments specific to Gα(s) or Gα(12) genes led to significantly reduced second messenger levels under PGE2, although the RNAi treatments specific to the other three Gα components did not. These results showed that the PGE2 receptor has high PGE2 affinity in the nanomolar range and binds G-proteins containing a Gα(s) or Gα(12) trimeric component in S. exigua and M. sexta, and likely, all lepidopteran insects.

Molecular Diagnostics for Monitoring Insecticide Resistance in Lepidopteran Pests

Chemical control methods to prevent crop damage have long been directly implicated in the selection of lepidoptera insect populations resistant to insecticides. More recently, new products featuring different modes of action (MoA), developed to mitigate the negative effects of control management on both producers and the environment, are rapidly losing efficacy due to the emergence of insect resistance. Among these, certain resistances are associated with molecular changes in the genomes of pest insects that are valuable for developing molecular markers for diagnostic tools, particularly the point mutations. Molecular diagnosis represents an innovative solution for insecticide resistance management (IRM) practices, allowing for the effective monitoring of insecticide resistance. This approach facilitates decision making by enabling the timely alternation between different modes of action (MoAs). In this context, this review focuses on the major lepidopteran pests that affect globally significant crops, discussing the impacts of insecticide resistance. It gathers literature on diagnostic methods; provides a comparative overview of the advantages of different techniques in terms of efficiency, cost, precision, sensitivity, and applicability; and highlights several novel diagnostic tools. Additionally, this review explores the coffee leaf miner, Leucoptera coffeella, as an applied model to illustrate potential approaches for more effective and sustainable control strategies.

Bacillus thuringiensis strains with high insecticidal activity against insect larvae of the orders Coleoptera and Lepidoptera

Abstract The concept of sustainable agricultural development is aimed at reducing the use of chemical pest control agents. Since the use of chemical pesticides poses a significant environmental threat, one of the possible solutions to overcome this problem is the study of biological approaches, in particular, the use of entomopathogenic microorganisms as biological pest control agents. Insecticidal activity of over 220 strains of Bacillus thuringiensis (Bt) and Lysinibacillus sphaericus from the collection of the Laboratory of Spore-Forming Microorganisms of the Microbial Depository Center of the Scientific and Production Center “Armbiotechnology” and 15 strains isolated from samples of dead insects (imago) of representatives of various genera of the Coleoptera order was studied. The pathogenic effect of the strains was assessed in relation to the insect species Tenebrio molitor, Zophobas morio, Leptinotarsa decemlineata Say and Pieris brassicae, belonging to the Coleoptera and Lepidoptera orders. Among the test objects, the representative of Lepidoptera Pieris brassicae demonstrated the highest degree of sensitivity to B. thuringiensis strains up to 100%, with some Bt strains causing 60–100% mortality of Tenebrio molitor and Zophobas morio larvae of different ages. The larvae of the beetle Leptinotarsa decemlineata Say were also partially susceptible, presenting mortality levels of between 31 and 60%. The article is devoted to the study of Bt bacterial strains that have pronounced insecticidal activity against insect larvae of the orders Coleoptera and Lepidoptera.

Unveiling symbiotic bacterial communities in insects feeding on the latex-rich plant Ficus microcarpa.

The diversity and health of insects that feed on plants are closely related to their mutualistic symbionts and host plants. These symbiotic partners significantly influence various metabolic activities in these insects. However, the symbiotic bacterial community of toxic plant feeders still needs further characterisation. This study aims to unravel bacterial communities associated with the different species of insect representing three insect orders: Thysanoptera, Hemiptera, and Lepidoptera, along with their predicted functional role, which exclusively feeds on latex-rich plant species Ficus microcarpa. By using 16S rRNA gene high-throughput sequencing, the analysis was able to define the major alignment of the bacterial population, primarily comprising Proteobacteria, Firmicutes, Bacteroidota, Actinobacteriota, and Acidobacteriota. Significant differences in symbiotic organisms between three insect groups were discovered by the study: hemipterans had Burkholderia and Buchnera, and lepidopterans had Acinetobacter. At the same time, Pseudomonas was detected in high abundance in both lepidopteran and thysanopteran insects. Furthermore, these symbionts exhibit consistent core functions, potentially explaining how different insects can consume the same host plant. The identified core functions of symbionts open avenues for innovative approaches in utilising these relationships to develop environment-friendly solutions for pest control, with broader implications for agriculture and environmental conservation.

A near chromosome-level genome assembly of a ghost moth (Lepidoptera, Hepialidae)

Ghost moths are an unusual family of primitive moths (Lepidoptera: Hepialidae) known for their large body size and crepuscular adult activity. These moths represent an ancient lineage, frequently have soil dwelling larvae, and are adapted to high elevations, deserts, and other extreme environments. Despite being rather speciose with more than 700 species, there is a dearth of genomic resources for the family. Here, we present the first high quality, publicly available hepialid genome, generated from an Andean species of ghost moth, Druceiella hillmani. Our genome assembly has a length of 2,586 Mbp with contig N50 of 28.1 Mb and N50 of 29, and BUSCO completeness of 97.1%, making it one of the largest genomes in the order Lepidoptera. Our assembly is a vital resource for future research on ghost moth genomics.

Host adaptability of Microplitis manilae (Hymenoptera: Braconidae) in Noctuidae.

Biological control is an effective and sustainable method of integrated pest management. Microplitis manilae (Hymenoptera: Braconidae) is a common internal parasitoid wasp of the order Lepidoptera. To determine a suitable host species for the reproduction of parasitoids, it is essential to assess host suitability and parasitic potential of the parasitized pests. Herein, we investigated host selection and exploitation of M. manilae. We evaluated the parasitic efficacy of M. manilae in 4 pest species in the Spodoptera and Mythimna genera of the Noctuidae family. The results indicated that the parasitism rate of M. manilae on 3 species in the Spodoptera genus is higher than on Mythimna separata in the Mythimna genus, with M. manilae exhibiting a higher parasitism rate and shorter development duration on Spodoptera litura compared to other species. The parasitism rate for 1st instars hosts was 86.67 ± 0.04%, while the development duration was 14.1 ± 0.03 days. However, when parasitizing the 3rd instar of Spodoptera frugiperda, parasitoids showed a higher sex ratio, of 0.71 ± 0.05. Additionally, M. manilae had parasitic effect on M. separata, providing a new choice for its parasitism. The results identify the optimal host, which could enhance and reproduction rate and survival rate of M. manilae, thus facilitating their large-scale propagation. Understanding the parasitic effects of M. manilae on pests can further its field application, also plays a major role in promoting the development of biological control technologies and sustainable agricultural production.

Conserved fungal effector NLS1 suppresses Lepidoptera insect immunity by targeting the host defense protein Hdd11.

Entomopathogenic fungi have been widely used as the main mycoinsecticide for controlling agricultural and forest pests. The effector molecules of these mycopathogens have evolved to adapt to their hosts. The role of fungal effectors in evading the host immune system in insects remains mainly unclear. We characterized the widely distributed fungal effector necrosis-inducing-like secreted protein 1 (NLS1) in the entomopathogenic fungus Metarhizium robertsii. Our findings revealed the presence of M. robertsii NLS1 (MrNLS1) in host hemocytes during the early stage of hemocoel infection. MrNLS1 knock down (ΔMrNLS1) reduced fungal pathogenicity during infection and altered the expression of host immune genes. The molecular docking results and the yeast 2-hybrid assay confirmed that MrNLS1 interacts with the host defense protein Hdd11. The phylogenetic analysis indicated that Hdd11 is conserved across a broad range of Lepidoptera species. Knock down of hdd11 in Helicoverpa armigera, Bombyx mori, and Galleria mellonella markedly suppressed their immune responses against M. robertsii. However, no significant difference was observed in the mean lethal time between hdd11-knockdown Lepidoptera species infected with ΔMrNLS1 and those infected with wild-type M. robertsii. Therefore, in Lepidoptera insects, Hdd11 is essential for fungal defense. In conclusion, M. robertsii infects Lepidoptera insects by targeting host Hdd11 through its protein MrNLS1, thereby suppressing the host immune response. Our findings clarify the molecular mechanisms underlying fungal infection pathogenesis.

From Proline to Chlorantraniliprole Mimics: Computer-Aided Design, Simple Preparation, and Excellent Insecticidal Profiles.

Chlorantraniliprole (CHL), a favored agricultural insecticide, is renowned for its high efficiency and broad-spectrum effectiveness against lepidoptera insects. However, the urgency for new insecticide development is underscored by the intricate multistep preparation process and modest overall yields of CHL, along with the escalating challenge of insect resistance. In response, we have crafted CHL mimics from proline employing computer-aided drug design. Molecular docking analysis of CHL's interactions with the ryanodine receptor (RyR) revealed that the nitrogen atom within the pyrazole moiety does not engage in pivotal interactions. Its removal may not abolish bioactivity entirely but could substantially simplify the synthetic process, thereby enhancing atom economy. This revelation prompted the exclusion of nitrogen and the subsequent formation of a pyrrole ring, enabling the meticulous design of synthetic pathways characterized by cost-effective precursors, streamlined synthesis, the avoidance of toxic reagents, minimal instrumentation, and high yields in the pursuit of innovative RyR modulators. Among these modulators, A1 and B1, obtained with yields exceeding 60%, showcased exceptional insecticidal potency, with LC50 values spanning from 0.12 to 1.47 mg L-1 against P. xylostella and M. separate. The inhibitory effects of these two compounds on insect detoxification enzymes imply a reduced likelihood of eliciting resistance in comparison to CHL, a finding further corroborated by their insecticidal potency against resistant pests. Moreover, molecular docking, MD simulations, and DFT calculations provided valuable structural insights, potentially unraveling the superior insecticidal activity of these two molecules, and thus paving the way for developing more potent insecticides.

Molecular characterization and identification of insect pollinators of Valeriana jatamansi Jones from Shimla Hills, Western Himalaya, India

Valeriana jatamansi Jones a perennial medicinal herb belongs to the family Caprifoliaceae and highly pollinated by wild insect pollinators. In present investigation a total of 51 species of flower visitors were reported on V. jatamansi at altitude range of 1,927 to 2,850 m, in different localities of Shimla Hills, Western Himalaya. The collected insect pollinator species were taxonomically identified and 29 of them were olecularly characterized and identified using the cytochrome c oxidase subunit 1 (COI) sequence from mitochondrial DNA (mtDNA). BLAST analysis revealed 98 to 100 per cent similarity with the existing GenBank sequences. The average AT content was found to be significantly higher (69.8%) compared to the GC content (30.0%), indicating AT bias in all sequences. Phylogenetic analysis of 29 different species of insect ‘pollinator of V. jatamansi revealed two clades, one shows phylogenetic relationship between 28 species which belongs to four orders Diptera, Hymenoptera, Hemiptera and Lepidoptera and the other shows the one species which belongs to order Coleoptera. This study contributes to increasing the number of published accounts of NCBI and helps to accurately distinguish the pollinator fauna of Valeriana Jatamansi.

EpOME mediates the immune resolution and its alkoxide analog enhances the virulence of microbial insecticides against the legume pod borer, Maruca vitrata

Excessive and unnecessary immune responses cause serious adverse effects due to self-tissue damage and energy consumption, particularly at the late stage of infection to terminate the induced immunity. Unlike mammals, which use long-chain fatty acid oxylipins, C18 oxygenated polyunsaturated fatty acids are suggested to act as immune resolvins in insects, including two epoxyoctadecamonoenoic acids (9,10-EpOME and 12,13-EpOME). This study investigated the physiological roles of EpOMEs in immune resolution in the lepidopteran insect, Maruca vitrata. The levels of two EpOMEs in the larvae increased during the late infection stage upon immune challenge. At their peak concentrations at 96 h post-infection (pi), both EpOMEs were found at similar levels: 323.18 pg/mg body weight for 9,10-EpOME and 322.07 pg/mg body weight for 12,13-EpOME. Both EpOMEs inhibited cellular and humoral immune responses, with 12,13-EpOME being more potent than 9,10-EpOME. Genes associated with EpOME synthase and degradation, identified as Mv-CYP1 and Mv-sEH, were detected in various developmental stages and tissues of M. vitrata. RNA interference (RNAi) targeting Mv-CYP1 failed to inhibit the immune response, whereas RNAi targeting Mv-sEH enhanced the immunosuppression. In contrast to the acute (< 12 h pi) immune response involving eicosanoid biosynthesis, the expression of these two genes linked to EpOME metabolism increased significantly at the late infection stage (> 12 h pi). Several alkoxide analogs of EpOME, with the epoxide group replaced by an alkoxide group, were synthesized; one such derivative demonstrated substantially greater efficacy than the natural EpOMEs in inhibiting the immune response. Additionally, using EpOME alkoxide significantly increased the effectiveness of microbial insecticides. Moreover, exposing young larvae to sublethal doses of EpOME alkoxide or sEH inhibitor induced severe developmental delays. These results suggest a novel strategy for insect pest control using insect immune resolvin analogs.

Molecular Functions and Physiological Roles of Gustatory Receptors of the Silkworm Bombyx mori.

Complete elucidation of members of the gustatory receptor (Gr) family in lepidopteran insects began in the silkworm Bombyx mori. Grs of lepidopteran insects were initially classified into four subfamilies based on the results of phylogenetic studies and analyses of a few ligands. However, with further ligand analysis, it has become clear that plant secondary metabolites are important targets not only for Grs in the bitter subfamily but also for the Drosophila melanogaster Gr43a orthologue subfamily and Grs in the sugar subfamily. Gene knockout experiments showed that B. mori Gr6 (BmGr6) and BmGr9 are involved in the recognition of the feeding-promoting compounds chlorogenic acid and isoquercetin in mulberry leaves by the maxillary palps, suggesting that these Grs are responsible for palpation-dependent host recognition without biting. On the other hand, BmGr expression was also confirmed in nonsensory organs. Midgut enteroendocrine cells that produce specific neuropeptides were shown to express specific BmGrs, suggesting that BmGrs are involved in the induction of endocrine secretion in response to changes in the midgut contents. Furthermore, gene knockout experiments indicated that BmGr6 is indeed involved in the secretion of myosuppressin. On the other hand, BmGr9 was shown to induce signal transduction that is not derived from the intracellular signaling cascade mediated by G proteins but from the fructose-regulated cation channel of BmGr9 itself. Cryogenic electron microscopy revealed the mechanism by which the ion channel of the BmGr9 homotetramer opens upon binding of fructose to the ligand-binding pocket. Research on BmGrs has contributed greatly to our understanding of the functions and roles of Grs in insects.

A Vip3Af mutant confers high resistance to broad lepidopteran insect pests

A <scp>Vip3Af</scp> mutant confers high resistance to broad lepidopteran insect pests

Efficient CRISPR/Cas9-mediated genome editing in the European corn borer, Ostrinia nubilalis.

The European corn borer (Ostrinia nubilalis) is an agricultural pest and burgeoning model for research on speciation, seasonal adaptation and insect resistance management. Although previous work in O. nubilalis has identified genes associated with differences in life cycle, reproduction, and resistance to Bt toxins, the general lack of a robust gene-editing protocol for O. nubilalis has been a barrier to functional validation of candidate genes. Here, we demonstrate an efficient and practical methodology for heritable gene mutagenesis in O. nubilalis using the CRISPR/Cas9 genome editing system. Precise loss-of-function (LOF) mutations were generated at two circadian clock genes, period (per) and pigment-dispersing factor receptor (pdfr), and a developmental gene, prothoracicotropic hormone (ptth). Precluding the need for a visible genetic marker, gene-editing efficiency remained high across different single guide RNAs (sgRNA) and germline transmission of mutations to F1 offspring approached 100%. When single or dual sgRNAs were injected at a high concentration, gene-specific phenotypic differences in behaviour and development were identified in F0 mutants. Specifically, F0 gene mutants demonstrated that PER, but not PDFR, is essential for normal timing of eclosion. PTTH F0 mutants were significantly heavier and exhibited a higher incidence of diapause. This work will accelerate future studies of gene function in O. nubilalis and facilitate the development of similar screens in other Lepidopteran and non-model insects.

Detection of Coffee Leaf Miner Using RGB Aerial Imagery and Machine Learning

The sustainability of coffee production is a concern for producers around the world. To be sustainable, it is necessary to achieve satisfactory levels of coffee productivity and quality. Pests and diseases cause reduced productivity and can affect the quality of coffee beans. To ensure sustainability, producers need to monitor pests that can lead to substantial crop losses, such as the coffee leaf miner, Leucoptera coffeella (Lepidoptera: Lyonetiidae), which belongs to the Lepidoptera order and the Lyonetiidae family. This research aimed to use machine learning techniques and vegetation indices to remotely identify infestations of the coffee leaf miner in coffee-growing regions. Field assessments of coffee leaf miner infestation were conducted in September 2023. Aerial images were taken using remotely piloted aircraft to determine 13 vegetative indices with RGB (red, green, blue) images. The vegetation indices were calculated using ArcGis 10.8 software. A comprehensive database encompassing details of coffee leaf miner infestation, vegetation indices, and crop data. The dataset was divided into training and testing subsets. A set of four machine learning algorithms was utilized: Random Forest (RF), Logistic Regression (LR), Support Vector Machine (SVM), and Stochastic Gradient Descent (SGD). Following hyperparameter tuning, the test subset was employed for model validation. Remarkably, both the SVM and SGD models demonstrated superior performance in estimating coffee leaf miner infestations, with kappa indices of 0.6 and 0.67, respectively. The combined use of vegetation indices and crop data increased the accuracy of coffee leaf miner detection. The RF model performed poorly, while the SVM and SGD models performed better. This situation highlights the challenges of tracking coffee leaf miner infestations in fields with varying ages of coffee plants, different cultivars, and other environmental variables.

Chromosome-level genome assembly of predatory Arma chinensis

Arma chinensis is a natural enemy that preys on various species and can suppress agricultural and forest pests in the orders Lepidoptera and Coleoptera. Here, we aimed to determine the genome of A. chinensis assembled at the chromosome-level using PacBio and Hi-C technologies. The assembled genome was 986 Mb, with a contig N50 of 2.40 Mb, scaffold N50 of 134.98 Mb, and BUSCO completeness of 96.10%. Hi-C data aided in anchoring the assembly onto seven chromosomes. A sequence of ~ 496.2 Mb was annotated as a repeat element, constituting 51.15% of the genome. We functionally annotated 84.79% of 20,853 predicted protein-encoding genes. This high-quality A. chinensis genome provides a novel genomic resource for future research on Pentatomidae insects.

Utility of Cry1Ja for Transgenic Insect Control.

Insect control traits are a key component of improving the efficacy of insect pest management and maximizing crop yields for growers. Insect traits based on proteins expressed by the bacteria Bacillus thuringiensis (Bt) have proven to be very effective tools in achieving this goal. Unfortunately, the adaptability of insects has led to resistance to certain proteins in current commercial products. Therefore, new insecticidal traits representing a different mode of action (MoA) than those currently in use are needed. Cry1Ja has good insecticidal activity against various lepidopteran species, and it provides robust protection against insect feeding with in planta expression. For Bt proteins, different MoAs are determined by their binding sites in the insect midgut. In this study, competitive binding assays are performed using brush border membrane vesicles (BBMVs) from Helicoverpa zea, Spodoptera frugiperda, and Chrysodeixis includens to evaluate the MoA of Cry1Ja relative to representatives of the various Bt proteins that are expressed in current commercial products for lepidopteran insect protection. This study highlights differences in the shared Cry protein binding sites in three insect species, Cry1Ja bioactivity against Cry1Fa resistant FAW, and in planta efficacy against target pests. These data illustrate the potential of Cry1Ja for new insect trait development.

Species-specific evolution of lepidopteran TspC5 tetraspanins associated with dominant resistance to Bacillus thuringiensis toxin Cry1Ac

Transgenic crops producing insecticidal proteins from the bacterium Bacillus thuringiensis (Bt) have proven to be highly effective in managing some key pests. However, the evolution of resistance by the target pests threatens the sustainability of Bt crops. The L31S mutation in a tetraspanin encoded by HarmTspC5 (previously known as HaTSPAN1) has been shown to confer dominant resistance to the Bt protein Cry1Ac in Helicoverpa armigera, a globally damaging lepidopteran pest. However, the broader implications of the L31S mutation in the tetraspanins of other lepidopteran species remain unclear. The evolutionary analyses in this study indicate that TspC5s have evolved in a species-specific manner among the lepidopteran insects. To investigate the role of TspC5s in conferring dominant resistance to Cry1Ac, we used the piggyBac-based transformation system to generate four transgenic H. armigera strains that express exogenous TspC5 variants from three phylogenetically close species (Helicoverpa zea, Helicoverpa assulta and Heliothis virescens) and one phylogenetically distant species (Plutella xylostella). In comparison with the background SCD strain of H. armigera, the transgenic strains expressing HzeaTspC5-L31S, HassTspC5-L31S, or HvirTspC5-L31S exhibited significant resistance to Cry1Ac (10.0-, 21.4-, and 81.1-fold, respectively), whereas the strain expressing PxylTspC5-L27S remained susceptible. Furthermore, the Cry1Ac resistant phenotypes followed an autosomal dominant inheritance pattern and were closely linked to the introduced mutant TspC5s. These findings reveal the conserved role of TspC5s from Helicoverpa and Heliothis species in mediating the dominant resistance to Cry1Ac, and they provide crucial insights for assessing resistance risks related to mutant tetraspanins and devising adaptive resistance management strategies for these major lepidopteran pests.

A Nematode Isolate, Oscheius Tipulae, Exhibiting a Wide Entomopathogenic Spectrum and its Application to Control Dipteran Insect Pests.

An entomopathogenic nematode, Oscheius tipulae, was isolated from a soil sample. The identification of this species was supported by morphological and molecular markers. The nematode isolate exhibited pathogenicity against different target insects including lepidopteran, coleopteran, and dipteran insects. The virulence of this nematode was similar to that of a well-known entomopathogenic nematode, Steinernema carpocapsae, against the same insect targets. A comparative metagenomics analysis of these two nematode species predicted the existence of a combined total of 272 bacterial species in their intestines, of which 51 bacterial species were shared between the two nematode species. In particular, the common gut bacteria included several entomopathogenic bacteria including Xenorhabdus nematophila, which is known as a symbiotic bacterium to S. carpocapsae. The nematode virulence of O. tipulae to insects was enhanced by an addition of dexamethasone but suppressed by an addition of arachidonic acid, suggesting that the immune defenses of the target insects against the nematode infection is mediated by eicosanoids, which would be manipulated by the symbiotic bacteria of the nematode. Unlike S. carpocapsae, O. tipulae showed high virulence against dipteran insects including fruit flies, onion flies, and mosquitoes. O. tipulae showed particularly high control efficacies against the onion maggot, Delia platura, infesting the Welsh onion in the rhizosphere in both pot and field assays.

Nutritional Evaluation of Eri Silkworm Pupa Strains: Addressing Entomophobia and Promoting Entomophagy

Samia ricini widely known as Eri silkworm is a commonly reared lepidopteran throughout the year. Six different strains of Eri silkworm are present, named according to their body pattern and color - greenish blue zebra (GBZ), greenish blue spotted (GBS), greenish blue plain (GBP), yellow zebra (YZ), yellow spotted (YS) and yellow plain (YP). Apart from being reared mostly for Eri silk, this lepidopteran insect is also considered as a popular delicacy among the tribes of Northeast India, along with it being moderately famous among the locals too. The proximate analysis of the lyophilized samples was carried out as per AOAC (2015) guidelines and mineral analysis was carried out in atomic absorption spectroscopy. Among the six different strains, YS contained highest moisture (7.82 gm) and crude fiber content (18.8 gm), whereas, YZ showed highest amount of total ash (4.32 gm) and crude fat content (22 gm). Furthermore, carbohydrate content was seen to be highest in GBS (14.41 gm). Hence, this study is aimed to unveil the nutritional worth of the six distinct pupa strains of Eri silkworm in an effort to dispel entomophobia, and promote entomophagy owing to the nutritional benefit it offers.

The Insects Visitor on Wild Israeli Grass Flowers (Asystasia gangetica (L.) T. Anderson)

Insects are invertebrate fauna that are important in various ecosystems and have high diversity and adaptability to various habitats. Insect diversity plays an important role (pollinator, pest control, or natural enemy) in several sectors, such as agriculture, ecology, human health, and natural resources. Apart from their beneficial role, the presence of insects can also be detrimental to humans; for example, insects can cause damage to agricultural products. Therefore, this research aimed to determine the diversity and role of insect visitors to the flowers of the Israeli grass plant (Asystasia gangetica). The research was carried out using a scan sampling survey method around community settlements, especially locations that various wild plants still cover. The research results showed that ten species and 255 individual insects visited Israeli grass flowers, which were divided into five orders, namely Lepidoptera, Coleoptera, Hemiptera, Diptera, and Hymenoptera. The order Lepidoptera is a flower-visiting insect species with the highest abundance and is a pollinator. Flowering plants that grow wild, such as Israel grass, benefit society indirectly by being used as traditional medicine. However, they also positively impact the diversity of flower-visiting insects. The presence of flower-visiting insects that work around grass indirectly has the potential to conserve beneficial grass plants but is underestimated by the community.