The activities of amylases, proteinase, cellulase, lipase, peroxidase, catalase, polyphenol oxidase and glucose-6-phosphatase in mature, young and very young stages of Termitomyces robustus (Beeli) Heim and Lentinus subnudus Berk were determined. Apart from β-amylase and glucose-6-phosphatase, the activities of the assayed enzymes increased from very young to mature fruitbody stages. Moreover, while the young fruitbody showed the highest glucose-6-phosphatase activity out of the three mushroom stages, there was no distinct trend with respect to the β-amylase activities. For cellulase, the activity in the stipe was greater than that of the pileus, while for the other enzymes, the reverse was the case. The mature fruit-bodies show the greatest loss of flavour, whiteness and food nutrients during storage. The findings are discussed in relation to past work.