Length Of Ileum Research Articles

Effects of Dietary Supplementation With Bacillus subtilis, as an Alternative to Antibiotics, on Growth Performance, Serum Immunity, and Intestinal Health in Broiler Chickens.

Bacillus subtilis (B. subtilis) as in-feed probiotics is a potential alternative for antibiotic growth promoters (AGP) in the poultry industry. The current study investigated the effects of B. subtilis on the performance, immunity, gut microbiota, and intestinal barrier function of broiler chickens. A 42-day feeding trial was conducted with a total of 600 1-day-old Arbor Acres broilers with similar initial body weight, which was randomly divided into one of five dietary treatments: the basal diet (Ctrl), Ctrl + virginiamycin (AGP), Ctrl + B. subtilis A (BSA), Ctrl + B. subtilis B (BSB), and Ctrl + B. subtilis A + B (1:1, BSAB). The results showed significantly increased average daily gain in a step-wise manner from the control, B. subtilis, and to the AGP groups. The mortality rate of the B. subtilis group was significantly lower than the AGP group. The concentrations of serum immunoglobulin (Ig) G (IgG), IgA, and IgM in the B. subtilis and AGP groups were higher than the control group, and the B. subtilis groups had the highest content of serum lysozyme and relative weight of thymus. Dietary B. subtilis increased the relative length of ileum and the relative weight of jejunum compared with the AGP group. The villus height (V), crypt depth (C), V/C, and intestinal wall thickness of the jejunum in the B. subtilis and AGP groups were increased relative to the control group. Dietary B. subtilis increased the messenger RNA (mRNA) expression of ZO-1, Occludin, and Claudin-1, the same as AGP. The contents of lactic acid, succinic acid, and butyric acid in the ileum and cecum were increased by dietary B. subtilis. Dietary B. subtilis significantly increased the lactobacillus and bifidobacteria in the ileum and cecum and decreased the coliforms and Clostridium perfringens in the cecum. The improved performance and decreased mortality rate observed in the feeding trial could be accrued to the positive effects of B. subtilis on the immune response capacity, gut health, and gut microflora balance, and the combination of two strains showed additional benefits on the intestinal morphology and tight junction protein expressions. Therefore, it can be concluded that dietary B. subtilis A and B could be used as alternatives to synthetic antibiotics in the promotion of gut health and productivity index in broiler production.

Nano-Bio zinc synthesized by Bacillus subtilis modulates broiler performance, intestinal morphology and expression of tight junction's proteins

Nano-zinc particles was synthesized using the Bacillus subtilis and the particles were characterized by transmission electron microscopy (TEM) methods. Treatment was applied in a completely randomized design with three sources of zinc (zinc sulfate, organic zinc, and nano-bio zinc) along with a control diet nonsupplemented with zinc, resulting in a total of 4 treatments with 6 replicates of 10 chicks. Performance criteria, ileum morphometry and microbial population and jejunum tight junction proteins relative expression were evaluated during the trial. The particle size of the synthesized zinc nanoparticles ranges 80 to 350 nm, with crystalline spherical or roughly spherical forms. Inclusion of nano-bio zinc increased BW and improved FCR compared to the organic and ZnSO4. Zinc supplementation meaningfully (P< 0.01) changed ileum morphology and reduced ileum microbiota compared to non-supplemented control group. Relative length of duodenum, jejunum and ileum and relative weight of liver and bursa of Fabricius were reduced (P< 0.01), but relative weight of carcass, breast and thigh were increased by supplementation of zinc on d 42. Supplementation of zinc increased (P<0.01) the relative expression of claudin-1, occludin and zonula occluden-1 and reduced (P<0.01) claudin-5 and zonula occluden-2 on day 28. Claudin-1, claudin-5, occludin and zonula occluden-1 expression were higher (P< 0.01) in chicks fed organic and nano-bio zinc compared to the chicks fed ZnSO4 on d 42. Taken together, the results indicated that the biosynthesis of zinc bio-nanoparticles using Bacillus subtilis for poultry is feasible. Furthermore, the data reported herein demonstrated that nano-bio zinc can effectively improve performance, intestinal health and integrity.

The Effects of Probiotic from Hymenachne Acutigluma Silage in Feed to the Length of Small Intestine and Caeca in Pegagan Ducks

This study aims to determine the effect of giving probiotics from (hymenachne acutigluma) silage in feed to the length of smallintestine and caeca in Pegagan ducks. The object of this research is sixty 5 months Pegagan ducks. The research design used was a Completely Randomized Design (CRD) with 5 treatments and 4 replications. There is 3 Pegagan ducks as a variable unit: P0 (Control); P1 (Ration + Probiotics with a dose 0,2%); P2 (Ration + Probiotics with a dose 0.4%); P3 (Ration + Probiotics with a dose 0.6%); P4 (Ration + Probiotics with a dose 0.8%). The observed variables were length of small intestine, length of duodenum, length of jejunum, length of ileum, and length of caeca. The results of this study indicate that administration of probiotics from (hymenachne acutigluma) silage in feed can increase the percentage of small intestine length and caeca. The conclusion of this research is the provision of probiotics from (Hymenachne acutigluma) silage with a dose 0.2% can increase the percentage of small intestine length and caeca in Pegagan ducks.

Enterococcus faecium PNC01 isolated from the intestinal mucosa of chicken as an alternative for antibiotics to reduce feed conversion rate in broiler chickens

BackgroundThe development and utilization of probiotics had many environmental benefits for replacing antibiotics in animal production. Bacteria in the intestinal mucosa have better adhesion to the host intestinal epithelial cells compared to bacteria in the intestinal contents. In this study, lactic acid bacteria were isolated from the intestinal mucosa of broiler chickens and investigated as the substitution to antibiotic in broiler production.ResultsIn addition to acid resistance, high temperature resistance, antimicrobial sensitivity tests, and intestinal epithelial cell adhesion, Enterococcus faecium PNC01 (E. faecium PNC01) was showed to be non-cytotoxic to epithelial cells. Draft genome sequence of E. faecium PNC01 predicted that it synthesized bacteriocin to perform probiotic functions and bacteriocin activity assay showed it inhibited Salmonella typhimurium from invading intestinal epithelial cells. Diet supplemented with E. faecium PNC01 increased the ileal villus height and crypt depth in broiler chickens, reduced the relative length of the cecum at day 21, and reduced the relative length of jejunum and ileum at day 42. Diet supplemented with E. faecium PNC01 increased the relative abundance of Firmicutes and Lactobacillus, decreased the relative abundance of Bacteroides in the cecal microbiota.ConclusionE. faecium PNC01 replaced antibiotics to reduce the feed conversion rate. Furthermore, E. faecium PNC01 improved intestinal morphology and altered the composition of microbiota in the cecum to reduce feed conversion rate. Thus, it can be used as an alternative for antibiotics in broiler production to avoid the adverse impact of antibiotics by altering the gut microbiota.Graphic

Nano-Bio Selenium Synthesized by Bacillus subtilis Modulates Broiler Performance, Intestinal Morphology and Microbiota, and Expression of Tight Junction's Proteins.

A green and ecofriendly bio-based synthesis of nano selenium particles was performed using the Bacillus subtilis and the products were characterized by field emission scanning electron microscope (FESEM), dynamic light scattering (DLS) and transmission electron microscopy (TEM) methods. Dietary treatments included a control diet nonsupplemented with selenium and control diet supplemented with different sources of selenium (sodium selenite, organic Se, and nano-bio Se), resulting in a total of 4 treatments with 6 replicates of 10 chicks. Broilers were assessed for performance measures, ileum morphometry, and microbial population and jejunum tight junction proteins' relative expression. The particle size of the synthesized selenium nanoparticles ranges 40 to 150nm, with crystalline spherical shape. Inclusion of selenium increased body weight (BW) and improved FCR compared to the control diet (P < 0.05). Among the selenium sources, the highest BW were achieved in chicks fed sodium selenite or nano-bio Se. Selenium supplementation meaningfully (P < - 0.01) changed ileum morphology and reduced ileum microbiota. Inclusion of selenium increased the relative weight of the carcass, breast, and thigh and reduced the relative weight of the liver and bursa of Fabricius on day 42 (P < 0.01). The relative length of duodenum, jejunum, and ileum were increased on day 14 but reduced on day 42 by inclusion of selenium (P < 0.05). Supplementation of selenium increased (P < 0.01) the expression of claudin-1, occludin, and zonula occluden-1 and reduced (P < 0.01) the expression of claudin-5 and zonula occluden-2 on day 28. Inclusion of nano-bio selenium increased (P < 0.05) the expression of occludin, zonula occluden-1, and zonula occluden-2 and reduced (P < 0.05) the expression of claudin-5 compared to the organic selenium and sodium selenite on day 42. In conclusion, this data suggest feasibility of the biosynthesis of selenium nanoparticles by Bacillus subtilis. Additionally, the data reported herein demonstrate that nano-bio selenium can effectively improve performance and intestinal integrity compared to the common organic and inorganic sources of selenium.

The effect of grit administration on the macroscopic and microscopic appearance of starter phase broilers digestive tract

Digestive tract has a key role in poultry growth due to its function in absorbing nutrients. Grit is one of feed ingredients widely used to accelerate grinding process. The purpose of this study was to determine the effect of grit administration on broiler chickens in the starter phase from age 1 to 21 days on the macroscopic and microscopic appearance of the digestive tract. The study design consisted of four combinations and five replications group from 200 broilers, with 10 broilers in each group. The treatments given were 0% grit; 0.5% grit of BW (body weight); 1% grit of BW; 1.5% grit of BW. The results showed that grit administration affected the macroscopic appearance of broilers digestive tract. Grit administration had a significant effect (P<0.05) on gizzard weight, duodenal length, jejunum length, ileum length, total intestinal length and total intestinal surface of broilers and had no significant effect (P>0.05) on the width of the small intestine. The results showed that grit administration affected the microscopic appearance of broiler chicken intestines. Grit administration had a significant effect (P<0.05) on the basal width and surface area of the small intestine villi and had no significant effect (P>0.05) on the villi apical width, villi height, and cryptic depth. In conclusion, grit administration grit administration to starter phase broilers can improve the macroscopic appearance of digestive tract and can improve the surface area of the small intestine villi with the optimal concentration 0.5% of body weight.

Effects of Resveratrol on Growth Performance, Intestinal Development, and Antioxidant Status of Broilers under Heat Stress.

Simple SummaryBroilers have unique physiological characteristics, no sweat glands and full of feathers, which makes it difficult to dissipate heat in high-temperature environments and is prone to heat stress (HS). HS has strong adverse effects on the meat production, growth performance, intestinal morphology, mortality and welfare of broilers, which can be alleviated by nutrition regulation. Resveratrol has been found to reduce the damage of HS on meat quality, immune and inflammatory response of broilers. However, there are few reports on the effects of resveratrol on the intestinal development and antioxidant capacity of broilers under HS. We demonstrated that resveratrol could improve the intestinal development and growth performance of broilers under HS. Besides, these findings suggest that resveratrol may offer an effective nutritional strategy to improve intestinal antioxidant function by regulating the expression of critical factors in the Nrf2 signaling pathway.The study investigated resveratrol’s effect on growth performance, intestinal development, and antioxidant capacity of broilers subjected to heat stress (HS). A total of 162 21-day-old male AA broilers were randomly divided into 3 treatment groups with 6 replicates of 9 birds each. The 3 treatment groups were as follows: the control (CON), in which broilers were housed at 22 ± 1 °C for 24 h day−1, and the HS and HS + resveratrol (400 mg/kg) groups, in which broilers were housed at 33 ± 1 °C for 10 h a day from 8:00 to 18:00 and 22 ± 1 °C for rest of the time. Results indicated that birds in the HS group exhibited lower (p < 0.05) final body weight (BW) and average daily gain (ADG) compared with birds in the CON group. HS birds also had lower (p < 0.05) relative jejunum weight, relative ileum and jejunum length, jejunal villus height, and villus height to crypt depth ratios than the CON group. The activities of glutathione peroxidase (GPX), glutathione S-transferase (GST), superoxide dismutase (SOD), and the mRNA levels of NF-E2-related factor 2 (Nrf2), SOD1, and GPX were also lower (p < 0.05) in the HS than CON group. The HS group had higher (p < 0.05) protein carbonyl (PC) contents and Kelch-like ECH-associated protein 1 (Keap1) mRNA levels. Compared with HS group, the HS + resveratrol group exhibited higher (p < 0.05) BW and ADG, relative jejunum weight, relative length of ileum, jejunal villus height, activities of GPX and GST, and mRNA levels of Nrf2 and SOD1, but they had lower (p < 0.05) PC content and Keap1 mRNA levels. In conclusion, resveratrol can improve the intestinal development and antioxidant function of broilers under HS, and therefore improve growth performance. The mechanism by which resveratrol enhances the intestinal antioxidant capacity is mediated by Nrf2 signaling pathway.

The effect of binahong leaf meal (Anredera cordifolia (ten.) Steenis) as feed additive on digestive organs profile of broiler chickens

Binahong (Anredera cordifolia (Ten.) Steenis) is known as an herb plant that has a biofunctional compound and is used for medical treatment. This study aims to determine the effect of binahong leaf meal (BL) on the digestive organs’ profile, namely intestinal length and weight (duodenum, jejunum, and ileum), and percentages of liver, heart, and bursal of broiler chickens. A total of one hundred and ninety-two male DOC of broiler were divided into six treatments with four replications, and each replication consisted of 8 birds. The treatment groups were: C (negative control), T: tetracycline 50 ppm (positive control), B1 (1% BLM), B2 (2% BL), B4 (4%), and B8 (8% BL). The data obtained were performed by analysis of variance and followed with Duncan’s multiple range test. The results showed that BLM’s addition increased to 8%, significantly different (P <0.01), to jejunum length, ileum length, liver and heart weight percentage. Based on our study, it can be concluded that the addition of BLM up to 4% produced the best performance on the broiler’s digestive organ profile.

Effect of black soldier fly, Hermetia illucens (Linnaeus) prepupae meal on growth performance and gut development in broiler chicken

An experiment was conducted to explore the suitability of black soldier fly (BSF) Hermetia illucens (Linnaeus) prepupae as poultry feed. Ninety day old, unsexed, Cobb chicks were distributed into 2 treatment groups, with 5 replicates, having 9 birds in each replicate, in a completely randomized design. The two dietary treatments consisted of corn-soy control diet and 5% supplemented insect meal-based diet. Results indicated similar (P > 0.05) body weight gain, feed intake or feed conversion ratio in chicks fed either insect meal or control diet during the experimental period. There was also no significant difference (P > 0.05) in the crude protein digestibility, cellular immunity as well as the length or weight of duodenum, jejunum, ileum and caecum between the treatments. It could be concluded that the black soldier fly prepupae meal can be incorporated at 5% in broiler diet. Thus, BSF can play a significant role in converting waste into feed and add to the feed basket in future.

Effect of â-mannanase supplementation and feed presentation on carcass characteristics and macro-mineral digestibility of growing rabbits fed palm kernel based diets

The influence of â -mannanase supplementation and feed presentation of palm kernel cake (PKC) based diets were assessed on grower rabbits. Twenty crossbred grower rabbits (757.5 ± 65g) of mixed sex aged between eight to ten weeks old were used during the trial in 2 X 2 factorial design conducted in a completely randomized experimental design giving four treatment interactions with five replicates of one rabbit per replicate. The treatments were; T1- diet in mash form without enzyme; T2- diet in pellet form without enzyme; T3- diet in mash form with â-mannanase (0.5g/kg) and T4- diet in pellet form with mannanase (0.5g/kg). The trial lasted for six weeks. The animals were weighed individually at the beginning of the study and weekly thereafter. At the end of the study period the feacal samples were collected and animals were slaughtered and measurements taken from the carcasses. The carcass yield showed no interaction, though full GIT weights (g) were significantly influenced (T1-23.04, T2-17.67, T3-19.99 and T4- 18.74. Enzyme inclusion and feed form elicited significant effects on carcass characteristics, with varying improvements observed. Enzyme inclusion on the mash diet gave lower organ weights compared to other diets. Feed form and enzyme inclusion influenced lengths of the segments of the GIT, with rabbit on Diet 3 having the longest length. Pelleting influenced ileum lengths positively. Rabbits fed pelleted or mash diets whether supplemented or non-supplemented with â-mannanase digested dry matter, calcium, phosphorus and magnesium in the same level. In conclusion, supplementation of diets with â-mannanase and pelleting of diets improved phosphorus digestibility in palm kernel cake based diets. In addition, rabbits feed mash and pelleted rations with addition of â-mannanase had improved loins as opposed to those fed mash and non-supplemented rabbits while other carcass and visceral organ characteristics were not significantly affected.

Evaluating whole grain millet feeding in broiler-starter chicks at 0-21days post hatch

A 21-d investigation was conducted to ascertain the effect of 50% whole millet grain replacement for milled millet on growth performance, gastrointestinal conditions and development in broiler chickens. Eighty-four one-day-old unsexed broiler chicks were randomly allotted to two treatment groups with 7 replicates of 6 chicks per replicate. Two diets; whole millet diet (WM), consisting of 50% of the millet provided as whole grain, and the ground millet diet (GM), as the control, were formulated and fed ad-libitum throughout the study period. Performance data: feed intake, daily live weight gain and feed conversion ratio were recorded weekly, i.e. at 7, 14 and 21d, while gastrointestinal assessment: jejunal, digesta pH, relative weights of the proventriculus, gizzard, pancreas and liver, and jejunal and ileal morphometry were conducted on 21d. Similarities (p &gt; 0.05) were observed in the growth performance, digesta pH and relative weight and length of duodenum, jejunum, ileum and caeca for chickens in both treatments. A 25.7% increase in gizzard weight was observed in chickens on WM, as well as significant (p &lt; 0.05) increases in jejunal and ileal height and jejunal villus width were recorded on the chickens. A significant reduction in flock uniformity was observed in chickens fed the whole millet diet at 14d. It could therefore be concluded that 50% whole millet grain replacement for milled millet resulted in similar growth performance and improved gastrointestinal function and activity in 21-d post hatch chickens.

Effect of cereal type and plant extract addition on the growth performance, intestinal morphology, caecal microflora, and gut barriers gene expression of broiler chickens

Feeding broiler chickens on diets based on cereal grains of high non-starch polysaccharides content such as wheat and barley can negatively impact their performance and gut health. Plant extracts can be used as a potential tool to alleviate these negative effects. The present study assessed the effects of dietary cereal type and the inclusion of a plant extract blend (PEB) on the growth performance, intestinal histomorphology, caecal microflora, and gene expression of selected biomarkers for gut integrity in broiler chickens in a 42-d experiment. Ross-308 male broilers were assigned into different dietary treatments and fed on two cereal types (corn- vs. wheat/barley-based) with/without added graded concentrations of a PEB (0, 250, 500, 1000, and 2000 mg/kg diet). There were no significant differences in the growth performance parameters, intestinal histomorphology, and caecal microflora due to the impact of dietary cereal type. However, lactobacilli count in the caecal microflora was increased in the group fed on a corn-based diet. The PEB supplementation especially at a level of 500 to 1000 mg/kg diet significantly increased the average BW and decreased the feed conversion ratio. It also increased the villi length of duodenum, jejunum, and ileum, decreased the duodenal crypt depth, and increased the villi length to crypt depth ratio in the duodenum, jejunum and ileum. Supplementation of the PEB decreased the total bacterial and coliform count and increased the lactobacilli count in a linear pattern. Gene expression of Occludin and Junction Adhesion Molecule was significantly increased in the PEB supplemented diets, whereby no influence was observed on mucin expression. In conclusion, supplementation of a PEB at levels of 500–1000 mg/kg can be used as a tool to improve broiler performance and gut health.

Modified Ileal Neobladder: Experience of 37 Cases

Bladder cancer is the second most common malignancy of genitourinary tract. About 20% cases are muscle invasive and most of the non-muscle invasive bladder cancer progressed to muscle invasion with time. The standard care for organ confined muscle invasive bladder cancer is radical cystectomy followed by urinary diversion. Orthotopic bladder substitution is considered standard urinary diversion after radical cystectomy in patients who have no urethral malignancy and intact external urethral sphincter. Neobladder constructed from ileum as described by Studer is considered standard and followed by most centers worldwide since 1985. We modified the procedure to improve the outcome and minimize post-operative complications by reducing the length of ileum used for construction of neobladder in 37 patients. Voiding, storage function and complications were evaluated during follow-up period up to 24 months after surgery. Three patients died during follow-up period of which 2 from metastatic disease and one from unrelated cause. Daytime and night-time continence rates were 86.49% and 78.38% respectively. Average functional capacity (437ml), maximum flow rate(15.7ml/s) and mean post void residual urine (44ml) during the period of evaluation. Clean intermittent self-catheterization needed in 67.57% patients due to incomplete emptying during early postoperative period but after 3 months most of the patients learnt to void without any aid. Upper tract dilatation found in one patient due to stricture at urethrovesical junction and required endoscopic incision and dilatation later. 12 patients had an urodynamic study at 12 months after surgery and found low pressure reservoir with small residual urine in all cases. Modified ileal neobladder is a safe and reliable procedure with satisfactory short-term result. Bangladesh Journal of Urology, Vol. 22, No. 1, January 2019 p.20-24

Dietary chitosan oligosaccharides alleviate heat stress–induced intestinal oxidative stress and inflammatory response in yellow-feather broilers

The purpose of this study was to evaluate the effects of chitosan oligosaccharides (COS) on intestinal permeability, morphology, antioxidant status, and inflammatory response in heat-stressed broilers. A total of 108 thirty-five-day-old Chinese yellow-feather broilers (body weight 470.31 ± 13.15 g) were randomly allocated to 3 dietary treatments as follows: CON group, basal diet and raised under normal temperature (24°C); HS group, basal diet and raised under cycle heat stress (34°C from 10:00–18:00 and 24°C for the rest time); HSC group, basal diet with 200 mg/kg COS supplementation and raised under cycle heat stress. Each treatment had 6 replication pens and 6 broilers per pen. Compared with the CON group, heat stress decreased (P < 0.05) the relative weight of duodenum and jejunum; the relative length and villus height (VH) of duodenum, jejunum, and ileum; the ileum VH to crypt depth ratio; duodenum mucosal catalase (CAT) activity; and jejunum mucosal glutathione peroxidase (GSH-Px) and CAT activity, whereas it increased (P < 0.05) serum diamine oxidase (DAO) activity and D-lactate acid (D-LA) content, duodenum and jejunum mucosal malondialdehyde (MDA) and interleukin-1β (IL-1β) content, and ileum mucosal tumor necrosis factor-α content. Compared to the HS group, dietary COS supplementation increased (P < 0.05) the relative length of duodenum, jejunum, and ileum; the VH of jejunum and ileum; and duodenum and jejunum mucosal GSH-Px activity, whereas it decreased (P < 0.05) serum DAO activity and D-LA concentration and duodenum and jejunum mucosal MDA and IL-1β content. These results suggested that dietary COS supplementation had beneficial effects on intestinal morphology by increasing jejunum and ileum VH; permeability by decreasing serum DAO activity and D-LA content; antioxidant capacity by decreasing duodenum and jejunum mucosal MDA content and by increasing duodenum and jejunum GSH-Px activity; and inflammatory response by decreasing duodenum and jejunum mucosal IL-1β content.

Ultrastructural intestinal mucosa change after prolonged inhibition of gastric acid secretion by omeprazole in male rats.

Omeprazole is a potent inhibitor of gastric acid secretion. It was reported that omeprazole induced dramatic gastric mucosa morphologic changes from the resting state to the stimulated state. However, the effect of omeprazole administration on the ultrastructure and absorptive function of small intestines was largely unknown. Here, male Sprague-Dawley rats were daily treated with a single dose of omeprazole for 12 or 24weeks. Ultrastructure intestinal mucosal change in duodenum, jejunum, and ileum was observed. We also determined small intestine inflammation, using intraepithelial lymphocytes activation. Finally, magnesium levels were measured in plasma, urine, feces, muscle, and bone to determine systemic magnesium balance. Omeprazole-treated rats had significantly decreased the width of tight junction, villous length, and absorptive area of duodenum, jejunum, and ileum compared to control rats. The small intestine of the omeprazole-treated group showed significantly higher intraepithelial lymphocytes activation levels compared with the control group. Lower secretory granules of Paneth cells at the base of the crypts were showed in omeprazole-treated rats. They also had significantly lower plasma, urinary, bone, and muscle Mg2+ contents indicating hypomagnesemia with systemic magnesium deficiency. In conclusion, prolonged omeprazole treatment-induced small intestinal inflammation and villous atrophy, which led to decrease small intestinal magnesium absorption in the condition of proton pump inhibitor-induced hypomagnesemia.

Ileocecal valve syndrome and vitamin b12 deficiency after surgery: a multicentric prospective study

Patients undergoing colon resection are often concerned about their functional outcomes after surgery. The primary aim of this prospective, multicentric study was to assess the intestinal activity and health-related quality-of-life (HRQL) after ileocecal valve removal. The secondary aim was to evaluate any vitamin B12 deficiency. The study included patients undergoing right colectomy, extended right colectomy and ileocecal resection for either neoplastic or benign disease. Selected items of GIQLI and EORTC QLQ-CR29 questionnaires were used to investigate intestinal activity and HRQL before and after surgery. Blood samples for vitamin B12 level were collected before and during the follow-up period. The empirical rule effect size (ERES) method was used to explain the clinical effect of statistical results. Linear mixed effect (LME) model for longitudinal data was applied to detect the most important parameters affecting the total score. A total of 158 patients were considered. Applying the ERES method, the analysis of both questionnaires showed clinically and statistically significant improvement of HRQL at the end of the follow-up period. Applying the LME model, worsening of HRQL was correlated with female gender and ileum length when using GIQLI questionnaire, and with female gender, open approach, and advanced cancer stage when using the EORTC QLQ-CR29 questionnaire. No significant deficiency in vitamin B12 levels was observed regardless of the length of surgical specimen. In our series, no deterioration of HRQL and no vitamin B12 deficiency were found during the follow-up period. Nevertheless, warning patients about potential changes in bowel habits is mandatory. In our series, no deterioration of HRQL and no vitamin B12 deficiency were found during the follow-up period. Nevertheless, warning patients about potential changes in bowel habits is mandatory.

PD41-02 SHORT SEGMENT ILEAL ORTHOTOPIC NEOBLADDER FOLLOWING RADICAL CYSTECTOMY FOR BLADDER CANCER: DOES SIZE MATTER?

You have accessJournal of UrologyTrauma/Reconstruction/Diversion: Ureter (including Pyeloplasty) and Bladder Reconstruction (including fistula), Augmentation, Substitution, Diversion II (PD41)1 Apr 2020PD41-02 SHORT SEGMENT ILEAL ORTHOTOPIC NEOBLADDER FOLLOWING RADICAL CYSTECTOMY FOR BLADDER CANCER: DOES SIZE MATTER? Anthony Devasia*, Arun George, Ramani Kumar, and Chandra Singh Anthony Devasia*Anthony Devasia* More articles by this author , Arun GeorgeArun George More articles by this author , Ramani KumarRamani Kumar More articles by this author , and Chandra SinghChandra Singh More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000000921.02AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Orthotopic neobladder is a diversion that is increasingly used following cystectomy for bladder cancer. The standard length of ileum used is 60 cm. We evaluated the functional outcomes of a short segment (35+5cm) ileal neobladder. METHODS: Following at least one side nerve sparing radical cystectomy for bladder cancer, orthotopic ileal neobladder was constructed using 35 cm of ileum for the pouch with an additional 5cm chimney. Preoperatively all patients were taught pelvic floor excercises and advised to perform them several times a day while awaiting surgery. Postoperatively frequent voiding was advised with alarm regulated voids at night keeping the voided volume to <400ml.Data was retrieved from our electronic database. Pouch capacity, voiding, continence, erectile function, upper tracts and biochemistry were assessed RESULTS: Of a total of 39 males who underwent the procedure from 2003 to 2019, an N pouch was constructed in 34. The mean age was 50.7 (SD 10.5)years and the mean follow up was 54 months. At 1 month, continence was 59% and at 3 months, 82.4% were fully continent with occasional nocturnal leak. By 6 months, all were continent not requiring pads with a rare leak of a few drops in a few, only at night. All men voided spontaneously without the need for clean catheterisation with a mean Q max of 22 ml/sec (SD 12), voided volume of 273ml (SD 109) and a residue of 30 ml(SD 23). There was no metabolic abnormality and renal function remained stable in all with no upper tract dilatation. Spontaneous erections were seen in 44% and 17% required PDE5 inhibitors. Six received chemotherapy (neoadjuvant 5, adjuvant 1). Two, in their late sixties who were fully continent for several years developed mild worsening of continence that improved after restarting pelvic floor exercises. Five years post operatively, one developed a calculus on the suture at the urethral anastomosis causing a ball valve effect, resulting in poor flow. The flow was restored after removal of the calculus. Thirty three (84.6%)were tumor free at the last review and 6 expired with late recurrence and metastases CONCLUSIONS: Orthotopic neobladder with a short segment of ileum (35+5) had good outcomes in terms of voiding to completion, continence and capacity, with biochemical and upper tract preservation in a carefully selected group. Source of Funding: nil © 2020 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 203Issue Supplement 4April 2020Page: e821-e821 Advertisement Copyright & Permissions© 2020 by American Urological Association Education and Research, Inc.MetricsAuthor Information Anthony Devasia* More articles by this author Arun George More articles by this author Ramani Kumar More articles by this author Chandra Singh More articles by this author Expand All Advertisement PDF downloadLoading ...

Growth, carcass characteristics, and meat quality of broilers fed a low-energy diet supplemented with a multienzyme preparation

The effect of a low-ME diet with a multienzyme (Kemzyme Plus, Kemin, Des Moines, IA) blend on performance, meat quality, and carcass traits was evaluated in Hubbard broiler chicks. A total of 120 Hubbard broiler chicks were allocated to the following 4 experimental groups and every group was separated into 6 replicates, with 5 birds per replicate: control (3,180 kcal/kg of ME), control + 0.50 g/kg diet of enzyme (Cont-Enz), low-ME diet (3,080 kcal/kg), and low-ME + 0.50 g/kg diet of enzyme (low-ME-Enz). The trail lasted for 16 D (32 to 48 D of age). No significant differences in growth parameters or carcass traits were observed among treatments. However, liver weight increased with the low-ME-Enz diet (P = 0.038). The low-ME diet recorded the highest weight for the bursa (P = 0.043) and thymus (P = 0.019). Dietary treatments had significant impacts on the length of duodenum, ileum, and cecum, as well as the weight of duodenum. The length of duodenum, ileum, and cecum increased with enzyme supplementation. The myofibril fragmentation index was lower with the Cont-Enz, low-ME, and low-ME-Enz diets than with the control diet (P = 0.043). The shear force increased with the low-ME-Enz diet (P = 0.022) than the control diet. Dietary treatments influenced breast meat yellowness (P = 0.019), whereas the low-ME diet had the lowest yellowness at the slaughtering age. The dietary treatments affected the breast meat pH (P = 0.001), with the control diet having the highest pH value after 24 hours. Thus, there was no effect of low-ME or enzyme supplementation to the control or low-ME diet on growth performance or carcass yield. However, feeding a low-ME diet or Cont-Enz preparation influenced organ and small intestine weights and meat characteristics.

Sodium butyrate as an effective feed additive to improve growth performance and gastrointestinal development in broilers.

This study was conducted to evaluate the effects of dietary sodium butyrate (SB) supplementation on growth performance, the development of gastrointestinal tract and immune organs (thymus, spleen and bursa of fabricius), and serum antibody titer after Newcastle disease (ND) vaccination in broilers. The total of 288 1‐day‐old broilers were randomly allocated to four groups with six replications according to initial body weight. Four treatment groups were designed as follows and fed the indicated diets: CON, basal diet; T1, basal diet supplemented with 0.3 g/kg SB; T2, basal diet supplemented with 0.6 g/kg SB; T3, basal diet supplemented with 1.2 g/kg SB. During days 1–21, broilers fed the T2 diet had higher (p < .05) average daily gain (ADG) than broilers fed the CON diet. On day 21, dietary SB supplementation showed linear increase (p < .05) in relative weight of the duodenum, jejunum, ileum, small intestine (the sum weight of duodenum, jejunum and ileum), pancreas and thymus, and linear increase (p < .05) in relative length of the duodenum, jejunum, ileum, small intestine (the sum length of duodenum, jejunum and ileum) and caeca. Meanwhile, dietary SB supplementation showed linear increase in the antibody titer against ND on days 14, 21, 28 and 35. In conclusion, dietary SB supplementation improved the development of gastrointestinal by increasing the relative weight and length, as well as enhanced the immune response of ND vaccine.

Sodium butyrate enhances growth performance and intestinal development in broilers

The aim of this study was to evaluate the effects of sodium butyrate (SB) on growth performance and development of digestive and immune organs in broilers. Dietary treatments had similar compositions but with 0%, 0.03%, 0.06%, and 0.12% SB substituted (weight/weight) for identical amounts of the basal diet. SB supplementation linearly increased (P &amp;lt; 0.05) average daily gain for each period, except for days 15–21. SB supplementation linearly increased (P &amp;lt; 0.05) the relative weight of proventriculus (day 7), gizzard (days 7 and 14), duodenum (days 21 and 28), jejunum (day 21), ileum (day 21), small intestine (day 21), rectum (day 14), pancreas (days 7 and 21), liver (days 21 and 28), and thymus (days 7, 14, and 21). SB supplementation linearly increased (P &amp;lt; 0.05) the relative length of duodenum (day 21), jejunum (days 14 and 21), ileum (days 14 and 21) and small intestine (days 14 and 21), caeca (day 21) and rectum (day 21), as well as it improved intestinal structure by increasing the villus height in jejunum and ileum, and increasing goblet cell counts in duodenum, jejunum, and ileum. Collectively, dietary SB supplementation improved the growth performance of broilers by improving the development and morphological structure of the broilers’ intestinal organs.