Introduction: Aortic pulse wave velocity (aPWV) is the gold-standard measure of arterial stiffness. In daily practice, the carotid-femoral PWV (cfPWV) is widely used for arterial stiffness assessment. PWV is calculated by dividing traveled distance by transit time. But, the inaccuracy of distance measurement and the approximation of the true aortic length which change with age may influence the absolute value of cfPWV. Making the assumption that the flow wave of continuous spectral Doppler corresponds to the real pulse wave, aPWV can be directly measured in the aorta by transesophageal echography (TEE). The aim of this study is to evaluate whether aPWV measured by Doppler technique during TEE is easily feasible and to analyse the reproducibility of the technique. Methods: Doppler images were obtained from the TEE examination of the thoracic aorta in 44 patients undergoing elective cardiac surgery. From a random choice of 194 acquisitions, a total of 100 images were analyzed offline by two different investigators in two separated periods to measure the transit time according to the two tangents method. Results: The Bland-Altman plots illustrate that there is no significant difference nor bias between the two readings made by one observer and neither for the mean of the observations made by separated observers. Intra-observer reproducibility of the Doppler measurements was high ( Intra Class Correlation (ICC) = 0.98 and 0.96). Agreement between observer was also good (ICC=0.98). Conclusions: These data demonstrate that Doppler technique during TEE is a feasible and reproducible method to calculate aPWV.