Response Of Legumes Research Articles

Contrasted response to climate change of winter and spring grain legumes in southwestern France

Abstract Climate change could undermine grain legumes ability to fix atmospheric nitrogen and their contribution to increase cropping systems sustainability. Pea (Pisum sativum L.) and faba bean (Vicia faba L.) are the two most widely grown grain legumes in Europe, yet the potential impact of climate change on their performances has not been quantified. We calibrated and evaluated the STICS soil-crop model for spring pea, winter pea and winter faba bean using experimental data from southwestern France and explored the effect of contrasting climate change scenarios. After calibration, STICS accurately simulated grain yield and amount of N2 fixed for the experimental growing seasons. Assuming no change in crop management, mean and inter-annual variability of grain yield and fixed N2 were assessed for historical (1995−2015), mid-term (2020−2040) and long-term (2060−2080) periods in one location in southwestern France. We considered projections from three climate models and two Representative [CO2] Pathways (RCP 4.5 and RCP 8.5). The climate models spanned a wide range of changes in temperature (+0.3 to +4.1 °C) and rainfall (−15% to +8%) depending on time horizon and RCP. Simulated grain yield increased over the long term in most scenarios (+1 to +25%), and spring pea tended to benefit less than winter pea and winter faba bean. Nevertheless, for the climate scenario with a decrease in rainfall and the strongest increase in temperature, simulated spring pea grain yield decreased by 28% while winter legumes yields were less affected (−14% for pea and no decrease for faba bean). Simulated changes in the amount of N2 fixed followed the grain yield response. Temperature rise caused a shortening in crop cycle duration. Simulated temperature stress significantly increased for spring and winter pea in most climate change scenarios while winter faba bean was rather unaffected due to greater upper temperature thresholds. N2 fixation of spring pea was reduced by above-optimal temperature during its vegetative growth in spring while N2 fixation of winter legumes was enhanced by the increase in temperature during their vegetative growth in winter. Simulated drought stress only increased in the climate scenario predicting a decrease in rainfall. Overall, [CO2] increase would allow offsetting negative effects of temperature and drought on grain yield and N2 fixation, except for climate scenarios involving a decrease in rainfall and the strong increase in temperature. The contrasted simulated response of winter and spring grain legumes to climate change in southwestern France points to the opportunity to tap grain legume diversity and cultivar choice as an adaptation strategy.

Potential effects of a high CO2 future on leguminous species.

Legumes provide an important source of food and feed due to their high protein levels and many health benefits, and also impart environmental and agronomic advantages as a consequence of their ability to fix nitrogen through their symbiotic relationship with rhizobia. As a result of our growing population, the demand for products derived from legumes will likely expand considerably in coming years. Since there is little scope for increasing production area, improving the productivity of such crops in the face of climate change will be essential. While a growing number of studies have assessed the effects of climate change on legume yield, there is a paucity of information regarding the direct impact of elevated CO2 concentration (e[CO2]) itself, which is a main driver of climate change and has a substantial physiological effect on plants. In this review, we discuss current knowledge regarding the influence of e[CO2] on the photosynthetic process, as well as biomass production, seed yield, quality, and stress tolerance in legumes, and examine how these responses differ from those observed in non-nodulating plants. Although these relationships are proving to be extremely complex, mounting evidence suggests that under limiting conditions, overall declines in many of these parameters could ensue. While further research will be required to unravel precise mechanisms underlying e[CO2] responses of legumes, it is clear that integrating such knowledge into legume breeding programs will be indispensable for achieving yield gains by harnessing the potential positive effects, and minimizing the detrimental impacts, of CO2 in the future.

Nitrogen fertilization and its legacy have inconsistent and often negative effect on plant growth in undeveloped post mining soils

Nitrogen supplement by adding N-fertilizer and/or growing of N-fixing trees are considered as important stimulus for the development of early vegetation. We performed pot experiments to determine the responses of legumes (N-fixing) and grasses (non-N fixing) in respect to their biomass to N fertilization, legacy of N, and combination of these two in various ages of post mining soils. N legacy was achieved either by long-term mineral N fertilization (legacy of inorganic N) (experiment 1) or growing of N2 fixing plant (legacy of N-fixing) (experiment 2). The first pot experiment was conducted to test N fertilization and legacy of inorganic N and these two on growth of Festuca rubra and Lotus corniculatus when grown separately. Following this, plant-soil feedback was defined as positive or negative by comparing the second generation of plant biomass in soils with and without plants in the previous generation. In the second pot experiment, we compared the performance of P. compressa and L. corniculatus in the presence of legacy of N-fixing and N fertiliaztion when grown together, and at the same time, plant-soil feedback was determined.N fertilization increased the growth of grasses, but only increased biomass of legumes in young soils. N-legacy induced variable plant growth, in particular often decreased the biomass of legumes. Legacy of inorganic N was only beneficial for grasses rather than legumes in medium soils. When growing in the same pot, legacy of N-fixing showed positive effects on grasses, depending on soil age and N fertilization. Repeated growing of plants in the same soil mostly resulted in negative plant-soil feedback. Negative plant-soil feedback was not alleviated by N fertilization and legacy of inorganic N for both grasses and legumes when grown separately. However, growing grasses and legumes together can induce positive plant-soil feedback with no legacy of N-fixing.In conclusion, N addition often stimulates plant growth more than long-term N effect. Legacy of N tends to be only beneficial for non-N fixing plants in medium aged soils. While negative plant-soil feedback occurs for grasses and legumes both grown separately or together, legumes can induce positive plant-soil feedback when grown with grasses in no legacy of N-fixing soils.

Yield and Nitrogen Uptake in Wheat and Chickpea Grown Under Elevated Carbon Dioxide Level

Increase in the atmospheric carbon dioxide (CO2) concentration has fertilization effect on crops if nutrient supply remains adequate. Response of cereals and legumes to increased CO2 concentration might differ due to the nitrogen fixing ability of leguminous crops. Considering the importance of differential response of cereal and legumes under elevated CO2 concentration, a field study was conducted to compare the effect of elevated CO2 on yield and plant nitrogen uptake in wheat and chickpea crop. Elevated CO2 level (550 ppm) increased yield by 15.1% and 16.7% over ambient in wheat and by 21.1% and 21.9% in chickpea (p ≤ 0.05) during the first and second years of the study. Nitrogen content in wheat grains decreased under elevated CO2 concentration. Chickpea, being a leguminous crop, showed no change in grain N content. However, higher biomass and grain yield resulted in higher N uptake in both the crops under elevated CO2 level. Under elevated CO2 concentration more partitioning of biomass toward seeds lead to higher seed N partitioning in chickpea. The study showed that although growth and yield of crops might increase in high CO2 condition, nitrogen concentration in grains and soil available N status might decrease in cereals like wheat. But chickpea might not get affected due to their ability to fix atmospheric N2.

Changes in environmental CO2 concentration can modify Rhizobium-soybean specificity and condition plant fitness and productivity

Over the past 10 years, it has been demonstrated in the literature that legume responses to elevated [CO2], whether positive, negative, or null, are in part dependent on the Rhizobium species and genotypes that establish symbiosis with the plant. However, all the strains used in these past experiments were isolated in field conditions at ambient [CO2]. We studied for first time the fitness response of soybean inoculated with a Rhizobium strain that has been previously isolated from nodules of plants grown at elevated [CO2] in field conditions at a FACE site. In experiments developed in controlled growth chambers, and in the field under ambient [CO2], the plants inoculated with the strain isolated at elevated [CO2] showed similar response as plants without inoculation. We hypothesize that deficient nodulation may be associated with a change in root exudates caused by the change in [CO2]. This study showed that the strains isolated in nodules at elevated [CO2] are not capable of properly nodulating soybean plants grown at ambient [CO2] and that the origin of strains do not ensure the performance of plants under the same conditions. However, more research is needed in order to understand how changes in environmental conditions can affect the symbiotic relationship and ultimately how we can improve plant fitness in a changeable world.

Water use of selected grain legumes in response to varying irrigation regimes

Grain legumes have potential to contribute to food and nutritional security in water-scarce areas. Information on their yield, water use and water productivity (WP) would be useful for their promotion. The aim of the study was to make a comparative assessment of adaptation, yield, water use and WP of an African indigenous grain legume (bambara groundnut) and two major grain legumes (dry bean and groundnut) under rainfed, deficit and optimum irrigation conditions. Field trials were conducted during the 2015/16 and 2016/17 summer seasons in KwaZulu-Natal, South Africa, using a split-plot design arranged in completely randomised blocks with three replications. Data collected included stomatal conductance, leaf area index, timing of key phenological stages and yield. Water use was calculated as a residual of the soil water balance. Water productivity was obtained as the quotient of grain yield and water use. Crops adapted to limited soil water availability through stomatal regulation and reduction in canopy size and duration. Yield, yield components and WP varied significantly (P < 0.05) among crop species. During 2015/16, groundnut had the highest yield and WP (10 540 kg·ha−1 and 0.99 kg·m−3, respectively). During 2016/17, the highest yield and WP were observed in dry bean, 2 911 kg·ha−1 and 0.75 kg·m−3, respectively. For both seasons, dry bean had the lowest water use (143–268 mm) across all water treatments. Dry bean and groundnut out–performed bambara groundnut with respect to yield, harvest index and WP. For any promotion of bambara groundnut as an alternative crop, there is need for crop improvement to improve yield and WP.

Ineos signs technology and catalyst agreement for new PDH unit in Europe

Over the past 10 years, it has been demonstrated in the literature that legume responses to elevated [CO2], whether positive, negative, or null, are in part dependent on the Rhizobium species and genotypes that establish symbiosis with the plant. However, all the strains used in these past experiments were isolated in field conditions at ambient [CO2]. We studied for first time the fitness response of soybean inoculated with a Rhizobium strain that has been previously isolated from nodules of plants grown at elevated [CO2] in field conditions at a FACE site. In experiments developed in controlled growth chambers, and in the field under ambient [CO2], the plants inoculated with the strain isolated at elevated [CO2] showed similar response as plants without inoculation. We hypothesize that deficient nodulation may be associated with a change in root exudates caused by the change in [CO2]. This study showed that the strains isolated in nodules at elevated [CO2] are not capable of properly nodulating soybean plants grown at ambient [CO2] and that the origin of strains do not ensure the performance of plants under the same conditions. However, more research is needed in order to understand how changes in environmental conditions can affect the symbiotic relationship and ultimately how we can improve plant fitness in a changeable world.

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Over the past 10 years, it has been demonstrated in the literature that legume responses to elevated [CO2], whether positive, negative, or null, are in part dependent on the Rhizobium species and genotypes that establish symbiosis with the plant. However, all the strains used in these past experiments were isolated in field conditions at ambient [CO2]. We studied for first time the fitness response of soybean inoculated with a Rhizobium strain that has been previously isolated from nodules of plants grown at elevated [CO2] in field conditions at a FACE site. In experiments developed in controlled growth chambers, and in the field under ambient [CO2], the plants inoculated with the strain isolated at elevated [CO2] showed similar response as plants without inoculation. We hypothesize that deficient nodulation may be associated with a change in root exudates caused by the change in [CO2]. This study showed that the strains isolated in nodules at elevated [CO2] are not capable of properly nodulating soybean plants grown at ambient [CO2] and that the origin of strains do not ensure the performance of plants under the same conditions. However, more research is needed in order to understand how changes in environmental conditions can affect the symbiotic relationship and ultimately how we can improve plant fitness in a changeable world.

DKSH cooperates with TNG in the exclusive marketing of titanium dioxide

Over the past 10 years, it has been demonstrated in the literature that legume responses to elevated [CO2], whether positive, negative, or null, are in part dependent on the Rhizobium species and genotypes that establish symbiosis with the plant. However, all the strains used in these past experiments were isolated in field conditions at ambient [CO2]. We studied for first time the fitness response of soybean inoculated with a Rhizobium strain that has been previously isolated from nodules of plants grown at elevated [CO2] in field conditions at a FACE site. In experiments developed in controlled growth chambers, and in the field under ambient [CO2], the plants inoculated with the strain isolated at elevated [CO2] showed similar response as plants without inoculation. We hypothesize that deficient nodulation may be associated with a change in root exudates caused by the change in [CO2]. This study showed that the strains isolated in nodules at elevated [CO2] are not capable of properly nodulating soybean plants grown at ambient [CO2] and that the origin of strains do not ensure the performance of plants under the same conditions. However, more research is needed in order to understand how changes in environmental conditions can affect the symbiotic relationship and ultimately how we can improve plant fitness in a changeable world.

The influence of phosphorus and calcium application rates on the mineral composition of the perennial legume Macrotyloma axillare

The response of legumes to the supply of either phosphorus (P) or calcium (Ca) alone has been extensively investigated but no prior studies have evaluated their combinations in the perennial legume Macrotyloma axillare (Macrotyloma legume). This study assessed the mineral composition of Macrotyloma legume in response to the combined application of P and Ca in an Ultisol. The experiment was conducted in a greenhouse in Nova Odessa city, São Paulo, Brazil. Five rates of P and Ca, respectively, were combined in a 52 incomplete fractional factorial design. Thus, the treatments consisted of 13 P and Ca combination rates (respectively, in mg dm−3): 0‒0, 0‒40, 0‒80, 15‒20, 15‒60, 30‒0, 30‒40, 30‒80, 45‒20, 45‒60, 60‒0, 60‒40 and 60‒80 arranged in a randomised block design with four replications. Mineral composition was evaluated after two harvests (53 d after sowing and 34 d after the first harvest). During the initial growth of the Macrotyloma legume, P × Ca interactions occurred. The P supply antagonistically influenced the nitrogen content. The excess P supply reduced nitrogen accumulation in the shoots and caused a lack of nitrogen in the leaves. The Ca supply synergistically impacted the magnesium content and caused a ‘Viets effect’ in potassium content.

Potential of Cool‐Season Species as Cover Crops and Forage in the Southeastern United States

Core Ideas Productivity of cool‐season cover crops depends on seasonal weather conditions. The annual grasses can produce substantial regrowth following winter harvest. Optimized growth termination justifies dual‐purpose plantings of cool‐season annuals. Cool‐season annuals can provide nutritious forage from late winter to early spring in addition to contributing soil conservation benefits during the winter season in the lower southern states. This application may reduce the direct input cost coming from adopting a winter cover crop in row crop production. A 2‐year field trial was conducted to determine the relative early‐season forage value of a variety of crops when planted for combined purposes of forage production and winter cover crops. The eight cover crop treatments included seven monocultures—annual ryegrass (Lolium multiflorum), cereal rye (Secale cereal), oats (Avena sativa), triticale (Triticale hexaploide), radish (Raphanus sativus), hairy vetch (Vicia villosa), and crimson clover (Trifolium incarnatum)—and a mixture of annual ryegrass/crimson clover/radish. Two harvests were made each year considering potential crop rotation. The dry matter (DM) yield ranged across cool‐season annual crop treatments from 1478 lb/acre for crimson clover in 2016 to 3983 lb/acre for oats in 2015. Delayed fall planting due to excessive rainfall in 2015 reduced DM yields and delayed growth for an initial harvest by almost 3 weeks compared with the first year. All treatments produced highly digestible forage, based on in vitro true digestibility (IVTD) analysis, ranging from 71–92%. As the growing season advanced, acid detergent fiber (ADF) in triticale increased at the greatest rate among the tested cover crops. Crude protein (CP) decreased more than 7 percentage points between harvests in the grasses and radish while CP response of legumes was not consistent between years. Dual‐purpose plantings of oats or an early maturing annual legume for combined cover crop contributions and forage production appear particularly appropriate for cropping systems with no‐till April planting into grazed‐out cover crop stubble.

Response of Grain Legumes to Phosphorus Application in the Guinea Savanna Agro-Ecological Zones of Ghana.

Grain legumes (cowpea, peanut, and soybean) play important roles in household food and income security in smallholder farming systems in the Guinea Savanna agro-ecological zones of Ghana. However, yields are low, rarely exceeding 600 kg ha–1, prompting the need to evaluate responses of grain legumes to P fertilizer applications for two seasons. Conducting P studies is critical to help farmers adopt economic-based recommendations. Treatments evaluated in 2015 for the three crops were (i) farmers’ practice (no input and planted by farmer); (ii) control (no input and planted by researcher), and (iii) triple super phosphate (TSP) fertilizer. However, for soybean, an additional two treatments (inoculant only and inoculant plus TSP fertilizer) were included. In 2016, the treatments were the same, except on-farm demonstrations were not conducted on cowpea. The demonstrations were laid out in a Randomized Complete Block Design with each demonstration rep-resenting a replicate within a region. On average, P-fertilizer application increased yields by 296; 527, and 390 kg ha–1 for cowpea, peanut, and soybean grains, respectively. On average over the two seasons, P-fertilizer increased yield by 9.85; 13.00, and 17.56 per kg ha–1 kg–1 P applied for cowpea, soybean, and peanut, respectively, and these applications were cost effective. Peanut showed little response to P in the Upper East Region compared with a greater response in the Northern and Upper West Regions, suggesting that benefits from P-fertilizer for peanut may be location-specific. On average, rhizobium inoculation increased grain yield by 157 kg ha–1 across the three regions and significantly positive effects of inoculation were observed in both seasons. Our results show that substantial increases in grain legume yield may be achieved by applying P fertilizers, but farmers cannot afford them because of their relatively high cost. Planting adapted and improved varieties and using rhizobium inoculants may provide the most economically viable and low risk options for increasing yields of grain legumes in the savanna agro-ecological zones of Ghana

Crop response to manure and fertilizer in Burkina Faso and Niger

Farmyard manure (FYM) is valuable for soil management, especially for soils with < 10 g kg−1 organic C in semi-arid West Africa. This study determined short-term FYM effects on yield and on response to N, P and K fertilizer for 20 trials in Niger and 28 trials in Burkina Faso involving six crops. The comparisons were of 0 and 2.5 Mg ha−1 yr−1 FYM applied in Niger, and of 0 and 5 Mg ha−1 FYM applied once in 2 years in Burkina Faso. Fertilizer and FYM application alone had little effect on yield in Niger but there was a synergistic effect of fertilizer P with FYM which included increased mean responses to P of, respectively: 0.22 and 0.43 Mg ha−1 for sorghum grain and fodder (Sorghum bicolor L.); 0.15 and 0.27 Mg ha−1 for cowpea grain and fodder; 0.16 Mg ha−1 grain for pearl millet (Pennisetum glaucum L.) when intercropped with cowpea (Vigna unguiculata L.); and 0.39 Mg ha−1 for groundnut fodder (Arachis hypogea L.). Application of FYM increased pearl millet response to N but decreased legume response to K fertilizer. In Burkina Faso, there was a mean grain yield increase of 0.29 Mg ha−1 yr−1 due to FYM and the effect of applying both FYM and fertilizer was additive except for a synergy of N fertilizer plus manure application for maize (Zea mays L.). Therefore, farmers should apply FYM and fertilizer together in Niger but these can be applied alone or together in Burkina Faso with mostly similar effects.

An overview of the role and significance of 15N methodologies in quantifying biological N2 fixation (BNF) and BNF dynamics in agro-ecosystems

Quantitative estimates of BNF are needed to improve our understanding of the ecology of N in the environment and aid efforts to improve agricultural N management. Static models based on the principle of 15N isotope dilution have been proposed to estimate the proportion of N in a N2-fixing species that is derived from the atmosphere via biological N2 fixation. Furthermore, equations have been developed to quantify the movement of biologically fixed N between neighboring species or from legumes to cereals in crop rotations. The present paper is structured to provide a comprehensive overview of these methods in a logical and systematic manner. While the relevant literature is vast, some aspects have fortunately been covered by recent in-depth reviews which will be identified and briefly summarized. The overview will emphasize the more practical indirect methodologies based either on artificial 15N enrichment or 15N depletion, or alternatively on 15N natural abundance. In considering methods used to estimate symbiotic dependence, the major structural division is whether or not a non-N2-fixing reference plant is employed, and approaches taken to remove this source of error are described. Four examples are provided to illustrate the contemporary success of 15N-based methods, one in basic research involving endophytic BNF, and three in applied research involving legume breeding for enhanced BNF, the response of legumes to climate change and biotic and abiotic factors affecting legume symbiotic performance.

Contrasting responses of crop legumes and cereals to nitrogen availability.

In nonagricultural systems, the relationship between intrinsic water-use efficiency (WUEi ) and leaf nitrogen (Narea ) is known to be stronger for legumes than for nonlegumes. We tested whether these relationships are retained for major agricultural legumes and nonlegumes. We compared the response to N nutrition of WUEi (and its component parts, photosynthesis (Asat ) and stomatal conductance (gs )) for legumes Cicer arietinum, Glycine max, Lupinus alba and Vicia faba, nonlegume dicots Brassica napus and Helianthus annus, and nonlegume cereals Hordeum vulgare and Triticum aestivum. Surprisingly, and in contrast to studied cereals and nonlegume dicots, Narea was positively related to photosynthesis in the legumes, explaining nearly half of the variance in Asat . WUEi was tightly coupled to Narea for agricultural legumes and nonlegume dicots, but not for cereal crops. Our analysis suggests that breeding efforts to reduce gs in legumes could increase WUEi by 120-218% while maintaining Asat at nonlegume values. Physiologically informed breeding of legumes can enhance sustainable agriculture by reducing requirements for water and N.

Nutrient Content and Nutritional Water Productivity of Selected Grain Legumes in Response to Production Environment.

There is a need to incorporate nutrition into aspects of crop and water productivity to tackle food and nutrition insecurity (FNS). The study determined the nutritional water productivity (NWP) of selected major (groundnut, dry bean) and indigenous (bambara groundnut and cowpea) grain legumes in response to water regimes and environments. Field trials were conducted during 2015/16 and 2016/17 at three sites in KwaZulu-Natal, South Africa (Ukulinga, Fountainhill and Umbumbulu). Yield and evapotranspiration (ET) data were collected. Grain was analysed for protein, fat, Ca, Fe and Zn nutrient content (NC). Yield, ET and NC were then used to compute NWP. Overall, the major legumes performed better than the indigenous grain legumes. Groundnut had the highest NWPfat. Groundnut and dry bean had the highest NWPprotein. For NWPFe, Zn and Ca, dry bean and cowpea were more productive. Yield instability caused fluctuations in NWP. Water treatments were not significant (p > 0.05). While there is scope to improve NWP under rainfed conditions, a lack of crop improvement currently limits the potential of indigenous grain legumes. This provides an initial insight on the nutrient content and NWP of a limited number of selected grain legumes in response to the production environment. There is a need for follow-up research to include cowpea data. Future studies should provide more experimental data and explore effects of additional factors such as management practices (fertiliser levels and plant density), climate and edaphic factors on nutrient content and NWP of crops.

Responses of legumes and grasses to non-, moderate, and dense shade in Missouri, USA. I. Forage yield and its species-level plasticity

Responses of legumes and grasses to non-, moderate, and dense shade in Missouri, USA. I. Forage yield and its species-level plasticity

Responses of legumes and grasses to non-, moderate, and dense shade in Missouri, USA. II. Forage quality and its species-level plasticity

From a series of shade tolerance screening trials conducted in an outdoor Shade Tolerance Screening Laboratory, 22 forages (16 grasses and 6 legumes) were selected for quality evaluation. The forages were grown under non-shade (100% of full sun, the control), moderate shade (45%), and dense shade (20%) with adequate water and nutrients and free of competition from other plants. All 22 forages had equal or higher percent crude protein (%CP) and CP yield (g pot−1) under moderate shade than in the control. Under dense shade, 22 and 18 forages had equal or higher %CP and CP yield than in the control, respectively. Under moderate and dense shade, 14 and 15 forages, respectively, maintained relative feed value (RFV) equal to that in the control. Legumes had higher %CP and RFV than grasses with no differences for CP yield. Relative distance plasticity index (RDPI), an indicator of a species’ adaptability to different environments, suggested grasses maintained RFV better than legumes under shade, while legumes were more resilient under shade in maintaining %CP than grasses. Our results suggest most grass and legume forages will maintain or have improved quality when grown in agroforestry practices with light to moderate shade compared to forages grown in open pastures.

Negative Impact Analysis of Pigment contents in Legumes in response to Sulphur dioxide Treatment

Negative Impact Analysis of Pigment contents in Legumes in response to Sulphur dioxide Treatment

Responses of tropical legumes from the Brazilian Atlantic Rainforest to simulated acid rain.

We investigated the morphological and anatomical effects of simulated acid rain on leaves of two species native to the Brazilian Atlantic Rainforest: Paubrasilia echinata and Libidibia ferrea var. leiostachya. Saplings were subjected to acid rain in a simulation chamber during 10days for 15min daily, using H2SO4 solution pH 3.0 and, in the control, deionized water. At the end of the experiment, fragments from young and expanding leaves were anatomically analyzed. Although L. ferrea var. leiostachya leaves are more hydrophobic, rain droplets remained in contact with them for a longer time, as in the hydrophilic P. echinata leaves, droplets coalesce and rapidly run off. Visual symptomatology consisted in interveinal and marginal necrotic dots. Microscopic damage found included epicuticular wax flaking, turgor loss and epidermal cell shape alteration, hypertrophy of parenchymatous cells, and epidermal and mesophyll cell collapse. Formation of a wound tissue was observed in P. echinata, and it isolated the necrosis to the adaxial leaf surface. Acid rain increased thickness of all leaf tissues except spongy parenchyma in young leaves of L. ferrea var. leiostachya, and such thickness was maintained throughout leaf expansion. To our knowledge, this is the first report of acidity causing increase in leaf tissue thickness. This could represent the beginning of cell hypertrophy, which was seen in visually affected leaf regions. Paubrasilia echinata was more sensitive, showing earlier symptoms, but the anatomical damage in L. ferrea var. leiostachya was more severe, probably due to the higher time of contact with acid solution in this species.