Noninvasive evaluation of global cardiac function using myocardial performance (Tei) index in murine model has not been well described. We were to assess global left ventricular (LV) function using Tei index and to determine the feasibility of Tei index in both human and mice. Forty hypertensive patients with concentric LV hypertrophy (LVH) on echocardiogram (Echo) and fifteen male mice with ascending aortic banded over 2 wk were studied using Echo. The LV Tei indices both in human with concentric LVH and in aortic banded mice were significantly higher than controls. Cutoff value of LV Tei index >0.44 in human had sensitivity 65% and specificity 95%, and cutoff value >0.49 in mice had sensitivity 100% and specificity 87.5%. The Tei index was a simple, noninvasive, and feasible Echo parameter to evaluate the global cardiac function both in human with concentric LVH and in aortic banded mice. Further study validating with invasive method is needed.