Event Abstract Back to Event The influence of caffeine on aspirin passage into the central nervous system in rats V. Vasovic1, V Jakovljevic1, Momir Mikov1, S Vukmirovic1*, N Stilinovic1 and Momir Mikov2 1 Medical faculty of Novi Sad, Department of Pharmaoclogy, Toxicology and Clinical Pharmacology, Serbia 2 Medical faculty of Novi Sad, Department of Occupational Medicine, Serbia Introduction. In this study the effect of caffeine on aspirin passage through the blood-brain barrier(BBB) into the central nervous system(CNS) was examined. Methods. The experiments were carried out on anaesthetized white rats(n=60); aspirin was given as a retrograde intra-arterial bolus injection(15sec) into the right axillary artery. This way of administration permitted the aspirin to be directed towards the brachiocephalic trunk, and then by blood circulation to CNS, avoiding, thus, the interference of peripheral organs to aspirin pharmacokinetics. In this study it was followed the dynamics of aspirin passage into the CNS. There were two groups of animals. To the animals of the control group it was subcutaneously given physiological solution 30min before the intra-arterial aspirin injection, whereas the animals of the test group received at the same time caffeine also subcutaneously. Caffeine and physiological solution were applied in equimolar doses. The animals were sacrificed by decapitation 30,60,90,120 and 240sec after the aspirin application. Blood samples were taken from the left jugular vein, and the brain, after rinsing, was divided into brainstem, cerebellum and cerebral hemispheres, in order to determine aspirin concentrations by standard spectrophotofluorimetric method. Results. The aspirin concentrations were significantly lower(30 times) in the CNS than in blood what is probably a consequence of aspirin dissociation at the physiological pH value. The central kinetics of aspirin indicated that there were two CNS compartments, one consisted of the brainstem and cerebellum, and the other consisting of the cerebral hemispheres. Caffeine did not affect control group significantly the aspirin concentrations either in blood or the brain compartments, compared to the control groups of animals. Conclusion. Thus, it can he concluded that caffeine has not a significant effect upon aspirin passage through BBB into the CNS, so that the analgesic effect of aspirin cannot he enhanced by facilitating passage its passage to the CNS. Keywords: Caffeine, Aspirin, Central Nervous System Conference: 8th Southeast European Congress on Xenobiotic Metabolism and Toxicity - XEMET 2010, Thessaloniki, Greece, 1 Oct - 5 Oct, 2010. Presentation Type: Poster Topic: Xenobiotic toxicity Citation: Vasovic V, Jakovljevic V, Mikov M, Vukmirovic S, Stilinovic N and Mikov M (2010). The influence of caffeine on aspirin passage into the central nervous system in rats. Front. Pharmacol. Conference Abstract: 8th Southeast European Congress on Xenobiotic Metabolism and Toxicity - XEMET 2010. doi: 10.3389/conf.fphar.2010.60.00211 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 28 Oct 2010; Published Online: 04 Nov 2010. * Correspondence: Dr. S Vukmirovic, Medical faculty of Novi Sad, Department of Pharmaoclogy, Toxicology and Clinical Pharmacology, Novi Sad, Serbia, sasavukmirovic@neobee.net Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers V. Vasovic V Jakovljevic Momir Mikov S Vukmirovic N Stilinovic Momir Mikov Google V. Vasovic V Jakovljevic Momir Mikov S Vukmirovic N Stilinovic Momir Mikov Google Scholar V. Vasovic V Jakovljevic Momir Mikov S Vukmirovic N Stilinovic Momir Mikov PubMed V. Vasovic V Jakovljevic Momir Mikov S Vukmirovic N Stilinovic Momir Mikov Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.