Lensless microscopy is an imaging technique that allows high-resolution imaging over a large field of view with a cost-effective design. Conventional lensless microscopy often utilizes multi-height phase retrieval and pixel-super-resolution algorithms to reconstruct high-resolution images, requiring mechanical stages for three-dimensional relative movements between a light source, camera, and sample. However, the excessive use of stages inevitably increases the bulkiness of the system and extends the image acquisition time. Here, we propose a motion-free lensless microscope that incorporates an RGB LED matrix array. A high-resolution holographic image is reconstructed from subpixel-shifted color images obtained with LED illuminations without any mechanical movement. Using a prototype system, we have demonstrated a spatial-bandwidth product of 30 megapixels with a resolution of 0.87 µm and a field of view of 24 mm2. The usability of the proposed method has been further tested for histopathologic examination. Our system features a compact and high-performance design with inexpensive optoelectronic elements, a conventional CMOS sensor and an LED matrix, which are well-aligned with the original design motivation of lensless imaging methods.
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