PURPOSE: The CRAVE (Cravings for Rest and Volitional Energy Expenditure) Scale measures the intrinsic desire for movement and sedentary behaviors as assessed “right now”. The purpose of this investigation was to evaluate reliability and construct validity of the CRAVE scale before, during and after a university lecture. METHODS: The CRAVE Scale and Thayer Activation-Deactivation (AD) Checklist were administered to 41 students (mean age 22.5±5.1 years; 26.8% non-Caucasian; 24.4% female) around a 50-minute lecture. CRAVE: 13 items (7-Rest & 6-Move), 1-10 Likert scale, given pre-, mid- and post-lecture. AD Checklist: 20 items, 1-10 Likert Scale, measures perceived energy, tiredness, tension and calmness, only assessed pre-lecture. Lectures were at either 9AM, 12PM or 3PM. A linear mixed effects model was used to compare pre-, mid- and post-lecture CRAVE Scores across the day. Correlations were calculated to evaluate CRAVE and AD Checklist relationships. RESULTS: Desire to Move: significantly higher post-lecture compared to pre (32.2±2.0 vs. 27.5±2.0, p=0.007) and higher than mid (28.5±2.0, p=0.034). Desire to Rest: lower post-lecture compared to pre (28.3±2.8 vs. 33.4±2.8, p=0.016) and lower than mid (33.1±2.8, p=0.019). Cronbach alpha coefficients for pre-, mid- and post-lecture (desire to Move: 0.90, 0.94, 0.93, respectively; Rest: 0.89, 0.94, 0.93). Inter-class correlations: Move=.85; Rest=.90. The desire to move pre- and mid-lecture were similar at different times of the day. The post-class desire to move was significantly higher at 3PM compared to 9AM (44.0±16.6 vs. 28.11±12.2, p=0.019). There were no differences in desire to rest based on time of day. Desire to move at baseline was significantly associated with energy (r=0.38, p=0.018) and calmness (r= -0.47, p=0.003). Desire to rest at baseline was significantly associated with energy (r= -0.38, p=0.026), tiredness (r= 0.48. p=0.003). Tension was unrelated to either move or rest. CONCLUSION: The desire to move significantly increased while the desire to rest significantly decreased across a lecture period. Desires to move/rest were correlated with energy (positively and negatively), but move was most strongly associated with calmness and rest with tiredness. Finally, the CRAVE Scale showed high internal consistency.