Lean Meat Percentage Research Articles

The prediction of lean meat and subcutaneous fat with skin content in pork cuts on the carcass meatness and weight

Early post-mortem, objective and non-destructive prediction of tissue distribution in the major pork cuts is a challenge for the meat industry. Mathematical models to predict pig carcass composition using total lean meat percentage and carcass weight were evaluated in this study. The data were obtained from 455 cold pig carcasses which were dissected according to the EU reference method; total lean meat percentage and carcass weight ranged from 42.45 to 69.21% and from 23.26 to 55.22 kg, respectively. Developed empirical models gave a reasonable fit to the experimental data and successfully predicted the carcass composition and tissue distribution in primal cuts. The second order polynomial models showed high coefficients of determination for prediction of experimental results (between 0.612 and 0.929), while the artificial neural network (ANN) model, based on the Broyden–Fletcher–Goldfarb–Shanno iterative algorithm, showed better prediction capabilities (overall r2 was 0.889). The newly developed software, based on ANN model is easy, fast, cheap and with sufficient precision for application in the meat industry.

Characteristics of Pig Carcass and Primal Cuts Measured by the Autofom Ⅲ Depend on Seasonal Classification.

The objective of this study was to investigate slaughtering performance, carcass grade, and quantitative traits of cuts according to seasonal influence by each month in pigs slaughtered in livestock processing complex (LPC) slaughterhouse in Korea, 2017. A total of 267,990 LYD (Landrace×Yorkshire×Duroc) pig data were used in this study. Results of slaughter heads, sex distribution, carcass weight, backfat thickness, grading class, total weight, and fat and lean meat percentages of each cut predicted by Autofom Ⅲ were obtained each month. The number of slaughtered pigs was the highest in early and late fall but the lowest in midsummer. Only in midsummer that the number of females was higher than that of castrates. During 2017, carcass weight was the lowest in late summer. Backfat thickness was in the range of 21–22 mm. In mid and late spring, pigs showed high 1+ grade ratio (37.05% and 36.15%, respectively). For traits of 11 cuts predicted by Autofom Ⅲ, porkbelly showed lower total weight, lean weight, and fat weight in midsummer to early fall but higher lean meat percentage compared to other seasons. Weights of deboned neck, loin, and lean meat were the highest in midfall compared to other seasons (p<0.05). In conclusion, characteristics of slaughtering, grading, and economic traits of pigs seemed to be highly seasonal. They were influenced by seasons. Results of this study could be used as basic data to develop seasonal specified management ways to improve pork production.

Effects of microencapsulated complex of organic acids and essential oils on growth performance, nutrient retention, blood profiles, fecal microflora, and lean meat percentage in weaning to finishing pigs

A total of 90 pigs (6.47 ± 0.27 kg; 21 d of age) were used in this 22 wk feeding trial to evaluate the effect of microencapsulated complex of organic acids and essential oils (MOE) on growth performance, nutrient retention, blood profile, fecal microflora, and lean meat percentage in pigs. Pigs were randomly distributed to one of three treatment groups (six replicate pens per treatment, five pigs per pen). Dietary treatments were CON, basal diet; MOE1, basal + 0.1% MOE; and MOE2, basal diet + 0.2% MOE. The average daily gain, average daily feed intake, and feed efficiency ratio were significantly different among treatments (P &lt; 0.05). The apparent total tract digestibility of dry matter was linearly increased (P &lt; 0.05) by MOE2. Red blood cells and immunoglobulin G were significantly different in pigs fed MOE (P &lt; 0.05). The Lactobacillus concentration linearly increased (P &lt; 0.05) in MOE2 treatments compared with other treatments. The meat color (a*, redness) and drip loss decreased linearly (P &lt; 0.05) with increasing level of MOE. In conclusion, MOE supplementation could improve growth performance, nutrient retention, blood profile, fecal microflora, and meat quality in weaning to finishing pigs.

A register-based study on associations between vaccination, antimicrobial use and productivity in conventional Danish finisher pig herds during 2011 to 2014

Reduction of antimicrobial use (AMU) in pigs is a priority to counteract development of antimicrobial resistance in animal and human pathogens. However, there is concern that Danish pig producers complying with official AMU restrictions might experience reduced herd health and productivity in the future, if alternative strategies are not available. Vaccination has been suggested as a strategy to prevent disease and minimise the need for antimicrobial treatments. The aim of this register-based study was to assess the associations between data on vaccination, productivity and AMU in Danish finisher herds over a 4-year period following initiation of the Yellow Card, which is a restrictive AMU control scheme. For each of the years 2011 to 2014, sow herds were grouped according to purchase patterns regarding Porcine Circovirus Type 2 (PCV2) (use/no use). For the sow herds (N = 179–433), additional information of purchases of vaccines against Mycoplasma hyopneumoniae (MYC), Actinobacillus pleuropneumoniae (APP), Porcine Reproductive and Respiratory Syndrome virus (PRRS) and Lawsonia intracellularis (LAW) was included. By use of movement data, finisher herds receiving pigs from the sow herds were tracked and included in the analyses. Finisher herds (N = 40–62) with register data on productivity (i.e. average daily weight gain, feed conversion rate, mortality and lean meat percentage) and data on prescriptions of antimicrobials measured in Animal Daily Doses/100 finishers/day as well as the proportion of parenteral AMU treatments out of all treatments (AMU-ratio) were included. Univariable combinations were tested for statistically significant associations (P < 0.05) and included in multivariable linear mixed-effects model for each of the six outcome variables representing productivity (N=4) or AMU (N=2). Herd number was included as a random effect to account for the herds appearing more than once. The variables representing PCV2, enrolment in the Danish Specific Pathogen Free (SPF) system, year, herd type and herd size were included as potential confounders. Vaccination against PRRS and higher AMU for finishers were associated with increased lean meat percentage, potentially due to disease outbreaks resulting in reduced growth of the pigs and lower carcass weight at slaughter in herds with PRRS. None of the other types of vaccines was associated with any of the productivity outcomes. Vaccination against PCV2, PRRS and APP were associated with higher levels of AMU, and vaccination against LAW with a higher AMU-ratio. This may be explained as some farmers preferring to take action soon after observing disease problems. No association was found between vaccination against MYC and AMU. Herds enrolled in SPF had significantly higher average daily weight gain than non-SPF herds.

A comparative study of four rumen buffering agents on productive performance, rumen fermentation and meat quality in growing lambs fed a total mixed ration

Controlling rumen fermentation using buffering agents could contribute to enhancing ruminant productivity and performance. This study was realized to investigate the effect of dietary supplementation of AcidBuf, sodium bicarbonate, calseapowder and WMC seaweed (Utva Lactuca extra) on the animal performance, volatile fatty acids, rumen pH, rumen histology and carcass characteristics of growing male Awassi lambs. A total of 60 lambs was divided into five groups. One group served as a control and fed only on a concentrate diet without any buffering, whereas the other four groups were fed the concentrate diet supplemented with 0.4% AcidBuf (Buf1), 0.4% AcidBuf plus sodium bicarbonate, 50 : 50 (Buf2), 0.4% calseapowder (Buf3) or 0.4% WMC Seaweed (Buf4) for 98 days. The feed conversion ratio was (P<0.05) improved in Buf2 compared to the control and other treatment groups. The propionic acid decreased, whereas butyric acid was increased in the treatment groups (P<0.05) compared to the control. The pH of the rumen fluid and the length of submucosa were (P<0.05) higher in Buf4 and Buf1, respectively, compared to the control. Hot and cold carcass weights were (P<0.05) higher in Buf4 compared to Buf1. Lean meat percentage and rib eye area were (P<0.05) higher in Buf4; while the fat percentage was (P<0.05) lower in Buf2 and Buf4 groups compared to the control. The lightness and yellowness of meat were (P<0.05) higher in Buf1 and Buf4 compared to the control. The meat pH was (P<0.05) higher in Buf3 and Buf4 compared to Buf2 (at 1 h) and control (at 24 h). The visceral depot fat (%) was reduced with Buf3 and Buf4 compared to the control. The results indicated that dietary supplementation of different buffering agents improved feed efficiency, rumen pH, carcass characteristics and decreased the body fat in growing Awassi lambs.

The effects of immunization against gonadotropin-releasing hormone on growth performance, reproductive activity and carcass traits of heavy weight gilts

Heavy weight gilts commonly show signs of oestrus during the late finishing phase, which results in a period of reduced feed intake and growth rate. Immunization against gonadotropin-releasing hormone (GnRH) (IM, immunocastration) was developed for finishing boars and recently extrapolated to females. Immunocastration acts by suppressing reproductive activity and improving the growth potential. The objective of this study was to evaluate the effects of IM on growth performance, reproductive activity and carcass characteristics of late finishing gilts. Seventy-two gilts (63.49 ± 0.39 kg) were either injected with saline (Intact) or immunized against GnRH (Immunized). The study consisted of three experimental periods: between the first to second immunization (V1 to V2, 15 to 19 weeks of age), from the second immunization to the beginning of daily boar exposure (DBE) (V2 to DBE, 19 to 21 weeks of age) and from the beginning of DBE to slaughter (S) (DBE to S, 21 to 25 weeks of age). Immunized gilts showed an overall increase (from 15 to 25 weeks) of 3.90 kg (P < 0.05) of live weight, 56 g (P < 0.05) of average daily gain (ADG) and 250 g (P < 0.001) of average daily feed intake (ADFI). Immunized gilts had a greater ADFI (+240 g, P < 0.05) and worse feed conversion ratio (+0.26, P < 0.05) from 19 (V2) to 21 weeks of age (before DBE). Furthermore, those females had higher feed intake (+410 g; P < 0.001) plus greater daily weight gain (+92 g; P < 0.05) from V2 to S, and from DBE to S (+470 g of ADFI, P < 0.001; +129 g of ADG, P < 0.01, respectively). Immunocastration had no effect on backfat thickness, lean meat percentage and weight, cold carcass yield or loin depth (P > 0.05). Immunized gilts showed 4.4% increased cold carcass weight (P < 0.01) and 10.6% greater gross flank weight (P < 0.001). Immunization against GnRH did not influence shoulder, collar, loin, belly or ham weights. Nor did it influence belly fat thickness, or meat, skin plus fat and bones yields of cold ham (P > 0.05). Immunocastration reduced ovarian and uterine weights by 82% (P < 0.001) and 93% (P < 0.001), respectively, and suppressed oestrus manifestation in all gilts in the immunized group (P < 0.001). These results indicate that immunization against GnRH is a promising tool for stimulating growth performance with no detrimental effects on carcass quality of heavy weight finishing gilts, by means of oestrus suppression.

Changes in carcass composition of Black Slavonian pigs and their crossbreds with Duroc during growth

Present study was carried out on 32 pure Black Slavonian (BS) pigs and 32 crossbreds of Black Slavonian and Duroc (BSxDu); number of barrows and gilts was equal in both groups. Every three months, 4 barrows and 4 gilts from both groups were slaughtered for the analysis of tissue and primal parts development in the series of subsequent dissection trials covering a total fattening period of 12 months. The results showed that crossing the BS pigs with Duroc resulted in significant increase in the development of main tissues and primal parts. Significantly higher live weight, carcass weight and lean meat percentage was reported in BS pigs compared to BSxDu pigs (P<0.001). The exception was lean meat percentage determined at the age of 3 months (P>0.05). BSxDu pigs entered the experiment at significantly higher live weight (P<0.05) than BS pigs; this relationship continued during entire growth period. Muscle and fatty tissues followed the similar patterns of growth. Muscle tissue was produced in higher amounts than fat in case of BS pigs; in BSxDu pigs, fat overgrew muscle at the later stage of fattening. Although BS pigs exhibited lower formation of muscle tissue than BSxDu, their carcasses were significantly leaner.

Exploration of methods for lamb carcass yield estimation in Canada

Different approaches were evaluated to improve the accuracy of carcass yield predictions of Canadian lamb carcasses using manually obtained measurements and dual-energy X-ray absorptiometry (DEXA). Several linear carcass measurements were obtained from a population of commercial lamb carcasses representative of the variability in Canadian slaughter plants (n = 155). Carcass measures were categorized into four sets according to when each measure could be obtained in the slaughter process. Each set of carcass measurements were subjected to stepwise regression and used to develop models for the estimation of lean meat and saleable yield percentages. Tissue depth measures (at the GR site) explained 44% of variation in lean meat yield in hot carcasses and 53% in cold carcasses. When additional parameters were included with cold GR, the regression model explained 61.9% of the variability in lean meat yield. Saleable yield predictions were less accurate (R2 &lt; 0.40); the greatest degree of variability was predicted when the model included ribeye area (R2 = 0.39). The DEXA scans obtained on carcass sides were able to predict about 78% of variability in carcass lean meat yield and 91% of fat content. This information could be used by the lamb meat industry to establish new carcass classification systems based on more accurate lean meat yield values.

Disruption of the ZBED6 binding site in intron 3 of IGF2 by CRISPR/Cas9 leads to enhanced muscle development in Liang Guang Small Spotted pigs.

Insulin-like growth factor 2 (IGF2) plays an important role in the development of the foetus and in post-natal growth and development. A SNP within intron 3 of porcine IGF2 disrupts a binding site for the repressor, zinc finger BED-type containing 6 (ZBED6), leading to up-regulation of IGF2 in skeletal muscle and major effects on muscle growth, heart size, and fat deposition. This favourable mutation is common in Western commercial pig populations, but is not present in most indigenous Chinese pig breeds. Here, wedescribed the efficient disruption of the ZBED6 binding site motif in intron 3 of IGF2 by CRISPR/Cas9 in porcine embryonic fibroblasts (PEFs) from the indigenous Chinese pig breed, Liang Guang Small Spotted pig. Disruption of the binding motif led to a drastic up-regulation of IGF2 expression in PEFs and enhanced myogenic potential and cell proliferation of PEFs. IGF2-edited pigs were then generated using somatic cell nuclear transfer. Enhanced muscle development was evident in one pig with biallelic deletion of the ZBED6 binding site motif, implying that the release of ZBED6 repression has a major effect on porcine muscle development. Our study confirmed the important effect of a mutation in the ZBED6 binding site motif on IGF2 expression and myogenesis, thus providing the basis for breeding a new line of Liang Guang Small Spotted pigs with improved lean meat percentage, a trait of great commercial value to pig producers.

Nondestructive Estimation of Lean Meat Yield of South Korean Pig Carcasses Using Machine Vision Technique.

In this paper, we report the development of a nondestructive prediction model for lean meat percentage (LMP) in Korean pig carcasses and in the major cuts using a machine vision technique. A popular vision system in the meat industry, the VCS2000 was installed in a modern Korean slaughterhouse, and the images of half carcasses were captured using three cameras from 175 selected pork carcasses (86 castrated males and 89 females). The imaged carcasses were divided into calibration (n=135) and validation (n=39) sets and a multilinear regression (MLR) analysis was utilized to develop the prediction equation from the calibration set. The efficiency of the prediction equation was then evaluated by an independent validation set. We found that the prediction equation—developed to estimate LMP in whole carcasses based on six variables—was characterized by a coefficient of determination (Rv2) value of 0.77 (root-mean square error [RMSEV] of 2.12%). In addition, the predicted LMP values for the major cuts: ham, belly, and shoulder exhibited Rv2 values≥0.8 (0.73 for loin parts) with low RMSEV values. However, lower accuracy (Rv(2)=0.67) was achieved for tenderloin cuts. These results indicate that the LMP in Korean pig carcasses and major cuts can be predicted successfully using the VCS2000-based prediction equation developed here. The ultimate advantages of this technique are compatibility and speed, as the VCS2000 imaging system can be installed in any slaughterhouse with minor modifications to facilitate the on-line and real-time prediction of LMP in pig carcasses.

Identification of Two Novel Single Nucleotide Polymorphisms in the Promoter Region of the Pig AMP Deaminase 1 Gene Associated with Carcass Traits.

The AMP deaminase 1 (AMPD1) gene plays an important role in purine nucleotide interconversion and energy metabolism. In this study, two novel single nucleotide polymorphisms (SNPs) (g.-626 G > A and g.-566 A>G) were detected in the proximal promoter region of the AMPD1 gene. The Chinese indigenous pig breeds (Meishan and Tongcheng) had higher frequencies of the G and A alleles than Western meat-type breeds (Landrace and Large White) at the g.-626 G > A and g.-566 A>G loci. The transcriptional activity of the AMPD1 promoter carrying the haplotype H1 (A-626G-566) was significantly (p < 0.05) higher than that of the haplotype H2 (G-626A-566). In addition, pigs with the haplotype combination H1H1 had significantly (p < 0.05) higher mRNA expression levels of the AMPD1 gene than those with haplotype combinations H1H2 and H2H2 in two different skeletal muscles. Association analyses revealed that the pigs with the haplotype combination H1H1 had significantly higher lean meat percentage values but lower average backfat thickness (ABT, cm), buttock fat thickness (cm), and thorax-waist fat thickness (cm) values than the pigs with the haplotype combinations H1H2 and H2H2. These findings demonstrate that the two novel SNPs in the promoter region of the AMPD1 gene have significant associations with pig fat deposition traits.

Bayesian estimation of direct and correlated responses to selection on linear or ratio expressions of feed efficiency in pigs

BackgroundThis study aimed at (1) deriving Bayesian methods to predict breeding values for ratio (i.e. feed conversion ratio; FCR) or linear (i.e. residual feed intake; RFI) traits; (2) estimating genetic parameters for average daily feed consumption (ADFI), average daily weight gain (ADG), lean meat percentage (LMP) along with the derived traits of RFI and FCR; and (3) deriving Bayesian estimates of direct and correlated responses to selection on RFI, FCR, ADG, ADFI, and LMP. Response to selection was defined as the difference in additive genetic mean of the selected top individuals, expected to be parents of the next generation, and the total population after integrating genetic trends out of the posterior distribution of selection responses. Inferences were based on marginal posterior distributions obtained from the Bayesian method for integration over unknown population parameters and “fixed” environmental effects and for appropriate handling of ratio traits. Terminal line pigs (n = 3724) were used for a multi-variate model for ADFI, ADG, and LMP. RFI was estimated from the conditional distribution of ADFI given ADG and LMP, using either genetic (RFIG) or phenotypic (RFIP) partial regression coefficients. The posterior distribution of the FCR’s breeding values was derived from the posterior distribution of “fixed” environmental effects and additive genetic effects on ADFI and ADG.ResultsPosterior means of heritability were 0.32, 0.26, 0.56, 0.20, and 0.15 for ADFI, ADG, LMP, RFIP, and RFIG, respectively. Selection against RFIG showed a direct response of − 0.16 kg/d and correlated responses of − 0.16 kg/kg for FCR and − 0.15 kg/d for ADFI, with no effect on other production traits. Selection against FCR resulted in a direct response of − 0.17 kg/kg and correlated responses of − 0.14 kg/d for RFIG, − 0.18 kg/d for ADFI, and 0.98% for LMP.ConclusionsThe Bayesian methodology developed here enables prediction of breeding values for FCR and RFI from a single multi-variate model. In addition, we derived posterior distributions of direct and correlated responses to selection. Genetic parameter estimates indicated a genetic basis for the studied traits and that genetic improvement through selection was possible. Direct selection against FCR or RFIP resulted in unexpected responses in production traits.

Evaluation of an automatic lean meat percentage quantification method based on a partial volume model from computed tomography scans

The quality of a pig carcass is mainly measured by the lean meat percentage (LMP), which can be virtually estimated from computed tomography (CT) scans. Different strategies exist to classify the CT voxels into tissues such as fat, lean and bone, being the thresholding-based methods the most commonly used. However, these methods are usually affected by the partial volume effect, and also by data variability, which is implicit from different CT scanners and protocols, since no standard behaviour has been defined. The aim of this paper is to extend an LMP quantification method which uses a partial volume model by adding a new step to detect the animal skin, and thoroughly evaluate the new approach by analysing each of its steps. The evaluation is performed by comparing the whole pipeline of the proposed approach with a simple thresholding method and a thresholding method with bone filling and skin detection, which is an intermediate step of the new pipeline. Five experiments have been designed to test how accurate are the results of the method regarding the LMP values computed from the manual dissection, as well as the robustness to data variability. Two different manual dissection methodologies have been tested: the partial dissection, which estimates the LMP using the lean of the four main cuts of the carcass plus the tenderloin, and the total dissection, which uses the lean of the twelve main cuts. A total of 146 half carcasses have been used for this study (105 using the partial dissection methodology, and 41 using the total dissection one). To evaluate the experiments, the LMP values virtually obtained from the three methods have been compared mostly with the LMP values from the manual dissection, computing the coefficient of determination R2 from the correlations, as well as the root mean square error of prediction by means of leave-one-out cross-validation. A statistical analysis is performed to resolve if two correlations are significantly different. The experiments’ results confirm the high accuracy of the proposed approach for the LMP estimation, and mainly its high robustness to data variability. The experiments also disclose that the detection of the animal skin and its classification as a new tissue, instead of classifying it as lean, improve the results. The evaluated method has demonstrated to be as effective as the thresholding method with bone filling and skin detection, and more robust to data variability than the other evaluated methods.

Enrichment tools for undocked heavy pigs: effects on body and gastric lesions and carcase and meat quality parameters

Two independent trials were carried out to assess the effect of two enrichment tools on the prevalence of skin and tail lesions recorded prior of slaughtering, lesions of the pars oesophagea (OGL) of the stomach, and on carcase and meat quality traits in Italian heavy pigs (body weight range: 25–160 kg). Eighty undocked barrows (Landrace × Large White) were used in two trials (20 pigs/experimental group, 5 pigs/pen). In Trial 1, the control group received a hanging metal chain (C1), while the other group received wood logs (WL) placed inside a metal rack. In Trial 2, the control group was provided with hanging chain (C2), while the pen of the other group was enriched with a vegetal edible block (EB) placed inside the metal rack. In both trials, no differences were observed in the prevalence and severity of skin, tail and gastric lesions (p > .05). In Trial 1, WL pigs presented lower backfat (p = .01), higher lean meat percentage (p = .03) and higher drip loss in the loin muscle (p = .02) than C1 pigs. Tail score and gastric lesions showed a moderate correlation (r = 0.42; p = .01) in Trial 1. Treatments had no effect on carcase or meat quality traits in Trial 2 (p > .05). In conclusion, the two enrichments provided did not affect body and gastric lesions, carcase and meat quality of Italian heavy pigs, if compared to the metal chains.

Long-term effects of castration, chronic intermittent social stress, provision of grass silage and their interactions on performance and meat and adipose tissue properties in growing-finishing pigs

In order to assess chronic stress in entire and castrated male pigs and to describe effects of a provision of grass silage in those pigs, a 2 × 2 × 2 factorial experiment comprising the factors castration, chronic intermittent social stress and provision of grass silage was carried out with 147 growing-finishing pigs from 25.6 to 102.1 kg body weight. The experimental design allowed investigating interactions between the three factors, but only few were statistically significant. Stress exposure consisted of repeated short-term confrontations with unfamiliar pigs and short-term separations. Carcasses of stress-exposed pigs had thicker backfat, lower lean meat percentage and a different fatty acids composition of the adipose tissue. While entire males differed strongly from castrates in performance, carcass characteristics and adipose tissue properties, we found no evidence for an increased level of chronic stress in entire males compared to castrates. Provision of grass silage increased stomach weight and reduced dressing percentage, but did not impair performance, adipose tissue properties or meat quality.

Effect of a live attenuated vaccine against Lawsonia intracellularis in weaned and finishing pig settings in Finland

BackgroundThe intracellular bacterium Lawsonia intracellularis is an important pathogen in modern swine production. The aim of this study was to investigate the effect of a live attenuated L. intracellularis vaccine (Enterisol Ileitis®) on the health and production parameters of weaned and finishing pigs in a commercial Finnish 850-sow farm with diagnosed L. intracellularis infection. The herd was free from enzootic pneumonia, swine dysentery, progressive atrophic rhinitis, sarcoptic mange and salmonellosis. Four weekly groups of approximately 500 piglets were included in the study for a total of approximately 2000 piglets. Half of these piglets were vaccinated at 3 weeks of age and the other half served as controls. The study piglets were ear-tagged with individual numbers and colour-coded and were individually weighed at weaning (4 weeks), delivery to the finishing farm (12–14 weeks) and at slaughter. Mortality, symptoms of diseases and medications of the study piglets were registered in the nursery and finishing unit. Feed conversion rate was calculated for the finishing period and lean meat percentage was measured at slaughter.ResultsVaccinated piglets had a higher live weight than unvaccinated piglets at delivery to the finishing unit (+ 1.18 kg, P = 0.002) and at slaughter (+ 3.57 kg, P < 0.001). The daily weight gain of vaccinated piglets was better than unvaccinated piglets in the nursery (+ 14.8 g/d, P = 0.013) and in the finishing unit (+ 30.9 g/d, P < 0.001). Vaccination had no effect on feed conversion rate or lean meat percentage (P = 0.102). Altogether, 3.9 and 4.6% of the pigs were medicated for different reasons in the vaccinated and control groups, respectively. The return on investment for the vaccination was calculated to be 0.41.ConclusionsImmunisation of piglets with a live attenuated L. intracellularis vaccine resulted in higher meat yield in pig production via significantly higher live weight and average daily weight gain in a Finnish specific pathogen-free setting.

Screening for candidate genes related with histological microstructure, meat quality and carcass characteristic in pig based on RNA-seq data.

ObjectiveThe aim of the present study was to identify genetic variants based on RNA-seq data, obtained via transcriptome sequencing of muscle tissue of pigs differing in muscle histological structure, and to verify the variants’ effect on histological microstructure and production traits in a larger pig population.MethodsRNA-seq data was used to identify the panel of single nucleotide polymorphisms (SNPs) significantly related with percentage and diameter of each fiber type (I, IIA, IIB). Detected polymorphisms were mapped to quantitative trait loci (QTLs) regions. Next, the association study was performed on 944 animals representing five breeds (Landrace, Large White, Pietrain, Duroc, and native Puławska breed) in order to evaluate the relationship of selected SNPs and histological characteristics, meat quality and carcasses traits.ResultsMapping of detected genetic variants to QTL regions showed that chromosome 14 was the most overrepresented with the identification of four QTLs related to percentage of fiber types I and IIA. The association study performed on a 293 longissimus muscle samples confirmed a significant positive effect of transforming acidic coiled-coil-containing protein 2 (TACC2) polymorphisms on fiber diameter, while SNP within forkhead box O1 (FOXO1) locus was associated with decrease of diameter of fiber types IIA and IIB. Moreover, subsequent general linear model analysis showed significant relationship of FOXO1, delta 4-desaturase, sphingolipid 1 (DEGS1), and troponin T2 (TNNT2) genes with loin ‘eye’ area, FOXO1 with loin weight, as well as FOXO1 and TACC2 with lean meat percentage. Furthermore, the intramuscular fat content was positively associated (p<0.01) with occurrence of polymorphisms within DEGS1, TNNT2 genes and negatively with occurrence of TACC2 polymorphism.ConclusionThis study’s results indicate that the SNP calling analysis based on RNA-seq data can be used to search candidate genes and establish the genetic basis of phenotypic traits. The presented results can be used for future studies evaluating the use of selected SNPs as genetic markers related to muscle histological profile and production traits in pig breeding.

Classification of trace elements in tissues from organic and conventional French pig production

This study assesses the impact of the farming system on the levels of copper, zinc, arsenic, cadmium, lead and mercury in pig tissues from three types of production (Organic (n = 28), Label Rouge (n = 12) and Conventional (n = 30)) randomly sampled in different slaughterhouses. All the concentrations were below regulatory limits. In muscles, Cu, Zn and As were measured at slightly higher levels in organic samples but no differences between organic and Label Rouge was observed. Livers from conventional and Label Rouge pig farms exhibited higher Zn and Cd contents than the organic ones, probably due to different practice in zinc or phytase supplementation of fattening diets. Principal component analysis indicated a correlation between Cu and As concentrations in liver and carcass weight, and between Zn and Cd liver levels and lean meat percentage. The linear discriminant analysis succeeded in predicting the farming process on the basis of the lean meat percentage and the liver Cd level.

Prediction of foal carcass composition and wholesale cut yields by using video image analysis

This work represents the first contribution for the application of the video image analysis (VIA) technology in predicting lean meat and fat composition in the equine species. Images of left sides of the carcass (n=42) were captured from the dorsal, lateral and medial views using a high-resolution digital camera. A total of 41 measurements (angles, lengths, widths and areas) were obtained by VIA. The variation of percentage of lean meat obtained from the forequarter (FQ) and hindquarter (HQ) carcass ranged between 5.86% and 7.83%. However, the percentage of fat (FAT) obtained from the FQ and HQ carcass presented a higher variation (CV between 41.34% and 44.58%). By combining different measurements and using prediction models with cold carcass weight (CCW) and VIA measurement the coefficient of determination (k-fold-R2) were 0.458 and 0.532 for FQ and HQ, respectively. On the other hand, employing the most comprehensive model (CCW plus all VIA measurements), the k-fold-R2 increased from 0.494 to 0.887 and 0.513 to 0.878 with respect to the simplest model (only with CCW), while precision increased with the reduction in the root mean square error (2.958 to 0.947 and 1.841 to 0.787) for the hindquarter fat and lean percentage, respectively. With CCW plus VIA measurements is possible to explain the wholesale value cuts yield variation (k-fold-R2 between 0.533 and 0.889). Overall, the VIA technology performed in the present study could be considered as an accurate method to assess the horse carcass composition which could have a role in breeding programmes and research studies to assist in the development of a value-based marketing system for horse carcass.

Comparison of the content of lean meat in pigs on farm and slaughter line

Measurement of lean meat on slaughter line and formation of price on the basis significantly contribute to the overall improvement of the quality and profitability of production and distribution of pork. The content of lean meat on live pigs was measured on farm using ultrasound device PIGLOG 105. While in slaughterhouse, the content of lean meat measured using Fat-O-Meater (FOM), two-point method (TP) and partial dissection. 59.30% of lean meat in vivo was estimated by the apparatus PIGLOG-105 one day before slaughter. It is 0.91% more then partial dissection and when compared to FOM and TP it is more 4.86% and 4.02%. Great deviation between PIGLOG-105 on one side and FOM and TP on other side indicated some error, and then partial dissection solved this mystery. After this study, slaughterhouse constructed new formulas for FOM in pig carcass classification. Regarding that, slaughterhouses which used FOM or similar equipment for measuring percentage of lean meat, should control results of the equipment described in this study, minimum twice a year.