Previous researchers have indicated the importance of body composition and muscle quality in athletic performance. However, body composition and muscle-quality measures in swimmers and divers over a training season have yet to be evaluated. To identify changes in body composition and muscle characteristics over a competitive season and identify relationships between these variables and performance in National Collegiate Athletic Association Division I swimmers and divers. Cross-sectional study. University laboratory. A total of 17 collegiate swimmers and divers (age = 18.6 ± 0.7 years, height = 175.8 ± 4.0 cm, body mass = 69.7 ± 7.0 kg). At preseason and postseason, body composition in each participant was assessed using dual-energy x-ray absorptiometry. Echo intensity and muscle cross-sectional area were determined from an ultrasound panoramic scan of the vastus lateralis muscle. Race times were obtained from the university athletic Web site. Lean mass (P = .016), arm lean mass (P = .008), and muscle cross-sectional area (P = .03) were higher at postseason, whereas body fat percentage (P = .041) and echo intensity (P = .0007) were lower at postseason. Performance improved from preseason to postseason in all event groups (sprinters, distance swimmers, and divers; P < .05). Body composition and muscle characteristics improved through 1 training season, which may have implications for performance. Quantifying body composition and muscle characteristics may be beneficial for professionals who work with athletes in order to improve performance and prevent injury.