Leakage Values Research Articles

A New Mass Spectroscopy-Based Method for Assessing the Periodontal–Endodontic Interface after Intracanal Placement of Biomaterials In Vitro

Optimizing the interface between biomaterials and dental hard tissues can prevent leakage of bacteria or inflammatory mediators into periapical tissues and thus avoid alveolar bone inflammation. In this study, an analysis system for testing the periodontal-endodontic interface using gas leakage and subsequent mass spectrometry was developed and validated using the roots of 15 single-rooted teeth in four groups: (I) roots without root canal filling, (II) roots with an inserted gutta-percha post without sealer, (III) roots with gutta-percha post and sealer, (IV) roots filled with sealer only, and (V) adhesively covered roots. Helium was used as the test gas, and its leakage rate was found by measuring the rising ion current using mass spectrometry. This system made it possible to differentiate between the leakage rates of tooth specimens with different fillings. Roots without filling showed the highest leakage values (p < 0.05). Specimens with a gutta-percha post without sealer showed statistically significantly higher leakage values than groups with a filling of gutta-percha and sealer or sealer alone (p < 0.05). This study shows that a standardized analysis system can be developed for periodontal-endodontic interfaces to prevent biomaterials and tissue degradation products from affecting the surrounding alveolar bone tissue.

Marker-Assisted Introgression of the Salinity Tolerance Locus Saltol in Temperate Japonica Rice

BackgroundRice is one of the most salt sensitive crops at seedling, early vegetative and reproductive stages. Varieties with salinity tolerance at seedling stage promote an efficient growth at early stages in salt affected soils, leading to healthy vegetative growth that protects crop yield. Saltol major QTL confers capacity to young rice plants growing under salt condition by maintaining a low Na+/K+ molar ratio in the shoots.ResultsMarker-assisted backcross (MABC) procedure was adopted to transfer Saltol locus conferring salt tolerance at seedling stage from donor indica IR64-Saltol to two temperate japonica varieties, Vialone Nano and Onice. Forward and background selections were accomplished using polymorphic KASP markers and a final evaluation of genetic background recovery of the selected lines was conducted using 15,580 SNP markers obtained from Genotyping by Sequencing. Three MABC generations followed by two selfing, allowed the identification of introgression lines achieving a recovery of the recurrent parent (RP) genome up to 100% (based on KASP markers) or 98.97% (based on GBS). Lines with highest RP genome recovery (RPGR) were evaluated for agronomical-phenological traits in field under non-salinized conditions. VN1, VN4, O1 lines were selected considering the agronomic evaluations and the RPGR% results as the most interesting for commercial exploitation. A physiological characterization was conducted by evaluating salt tolerance under hydroponic conditions. The selected lines showed lower standard evaluation system (SES) scores: 62% of VN4, and 57% of O1 plants reaching SES 3 or SES 5 respectively, while only 40% of Vialone Nano and 25% of Onice plants recorded scores from 3 to 5, respectively. VN1, VN4 and O1 showed a reduced electrolyte leakage values, and limited negative effects on relative water content and shoot/root fresh weight ratio.ConclusionThe Saltol locus was successfully transferred to two elite varieties by MABC in a time frame of three years. The application of background selection until BC3F3 allowed the selection of lines with a RPGR up to 98.97%. Physiological evaluations for the selected lines indicate an improved salinity tolerance at seedling stage. The results supported the effectiveness of the Saltol locus in temperate japonica and of the MABC procedure for recovering of the RP favorable traits.

Rootstock-mediated physiological and fruit set responses in pepper under heat stress

An increase in high temperature causes major losses in pepper yields, especially in greenhouses when extending the cropping season to late spring or summer in mild climate areas. Grafting has been identified as a possible tool to cope with this abiotic stress. The objective of this study was to analyze the heat stress impact on a sweet pepper variety grafted onto rootstocks with diverse heat stress tolerances to evaluate high-temperature effects on the leaf metabolism, pollen traits and fruit set. To do so, under two greenhouses conditions (28/22°C and 38/22°C for control and heat stress, respectively), we compared the variety grafted onto two rootstocks (VA/A57 and VA/A55, tolerant and nontolerant, respectively), and used varieties ungrafted (VA) and self-grafted (VA/VA) as controls. VA/A57 obtained the lowest electrolyte leakage, non-disturbed chlorophyll and carotenoids concentration values, increased ascorbic acid and phenols concentrations, and no hydrogen peroxide accumulation. These findings indicate better predisposition to overcome heat stress than other plant combinations. Such physiological responses in leaves conferred by the tolerant rootstock coincided with the highest proline concentration in anthers, and better pollen germination and fruit set compared to the other graft combinations. We conclude that grafting peppers onto a heat stress-tolerant rootstock, such as A57, could overcome negative high-temperature effects better than an ungrafted variety. Moreover, the better physiological performance noted in vegetative parts conferred by a heat stress-tolerant rootstock would also lead to better performance in the reproductive development phase. All this indicates that using tolerant rootstocks in pepper could be an interesting method to alleviate heat stress effects on this crop.

Biostimulation effects of linseed and citrus oils on growth, antioxidant enzymes activity, metabolic changes and water relations of Khaya senegalensis seedlings under drought stress

This study aim to use environmentally friendly material (Linseed and citrus oils) to reduce the harmful effect of water deficit on Khaya senegalensis plant due to its economic importance as a source of wood in addition to many uses of alternative medicine. Therefore, Khaya senegalensis plants were grown in green house with three irrigation intervals (5, 7 and 9 days) and oils foliar spray as linseed oil (Lin) at (0, 5, 7%) and citrus oils at (0, 1, 2%). The results indicated that the 9 days irrigation interval gave the lowest values of the most studied growth parameters, water relations (relative water content (RWC%), water retention capacity (WRC) and membrane stability index(MSI%), photosynthesis pigments, chlorophyll stability index (CSI%), minerals content, total free amino acid while increased values of electrolyte leakage (EL%), total sugar, total phenol, and antioxidant enzymes activities(Catalase (CAT), Peroxidase (POX), Superoxide dismutase (SOD)). Both oils treated especially linseed oil, at 7% increased values of all parameters and chemical composition compared with untreated plants plus different irrigation intervals, except EL% was decreased. The data provided evidence that linseed and citrus oils treatment reduces the adverse effect of water deficit on Khaya senegalensis plants and can play a role in providing stress tolerance.

Farklı irrigasyon tekniklerinin kalsiyum hidroksit uzaklaştırma etkinliğinin Bilgisayarlı Sıvı Filtrasyon methodu kullanılarak mikrosızıntılarının değerlendirilmesi

Aim: To compare the efficiency of sonic irrigation, passive ultrasonic irrigation, and conventional syringe irrigation (CI) in removing pure and injectable calcium hydroxide (CH) from the root canals of mandibular second premolars by measuring the probable microleakage of residual CH particles in root canal obturation using computerized liquid filtration (CLF). Materials and Methods: Eighty instrumented mandibular second premolars were categorized into three experimental groups (n = 20 each) based on the irrigation method used for removing CH and a control group (n = 20) in which CH was not used. Each experimental group was divided into two equal groups (pure and injectable CH). After 1 week, CH was removed from the root canals, and the teeth were obturated. CLF measurements were performed 1 week after obturation. Nonparametric variables between two groups were compared using the Mann-Whitney U test, and the Kruskal-Wallis test was used to compare more than two groups. The significance level was set at p &amp;lt; 0.05. Results: All experimental groups showed higher leakage values than the control group. The highest leakage values were evident in the CI group, and the lowest leakage values were found in the sonic (EDDY) irrigation group. No significant differences were detected among the different forms of CH. Conclusions: None of the analyzed techniques could completely remove CH from root canals, resulting in higher leakage values. Sonic irrigation with EDDY was the best in removing CH from root canals.

Airtightness evaluation of Canadian dwellings and influencing factors based on measured data and predictive models.

The airtightness of buildings has a significant impact on buildings' energy efficiency, maintenance and occupant comfort. The main goal of this study is to provide an evaluation of the air leakage characteristics of dwellings in different regions in Canada. This study evaluated the key influencing factors on airtightness performance based on a large set of measured data (73,450 dwellings located in Canada with 11 measurement parameters for each). Machine learning models based on multivariate regression (MVR) and Random Forest Ensemble (RFE) were developed to predict the air leakage value. The RFE model, which shows better results than MVR, was used to evaluate the effect of the ageing of buildings. Results showed that the maximum increase in air leakage occurs during the first year after construction - approximately 25%, and then 3.7% in the second year, after which the increase rate becomes insignificant and relatively constant - approximately 0.3% per year. The findings from this study can provide significant information for building designs, building performance simulations and strengthening standards and guidelines policies on indoor environmental quality.

Electrical characteristics investigation of ferroelectric memories using stacked and mixed hafnium zirconium oxides

• Ferroelectric characteristics of mixed and stacked HfZrO devices were investigated. • Stacked HfZrO device showed a much stronger ferroelectricity than mixed one. • A surface nitrogen plasma treatment can alleviate ferroelectric domain pinning. • Nitrogen incorporation into stacked HfZrO further improved the endurance cycling. In this work, we investigate ferroelectric characteristics of hafnium zirconium oxide (HfZrO) metal-ferroelectric-metal (MFM) devices with mixed and stacked film structure. The experimental results reveal that the stacked HfZrO MFM device has a lower dynamic leakage and stronger ferroelectric polarization value of approximately 27.3 μC/cm 2 , which is much higher than 17.5 μC/cm 2 measured from mixed HfZrO MFM device. From endurance cycling test, we also confirm that the stacked HfZrO MFM capacitor with a remote nitrogen plasma treatment featuring a large initial hysteresis window can achieve a stable operation up to 10 8 cycles at an operating voltage of 2.5 V, which is even longer than that of mixed HfZrO MFM capacitor by one order of magnitude. Therefore, the stacked MFM structure with proper interface engineering provides a promising approach for the process integration of ferroelectric memory under the comprehensive consideration of device performance and endurance.

Risk factors of adjacent vertebral refracture after percutaneous vertebroplasty for osteoporotic vertebral compression fractures in super-old patients

To analyze the risk factors for refracture of adjacent vertebrae after percutaneous vertebroplasty (PVP) in super-old patients with osteoporotic vertebral compression fractures(OVCFs). A retrospective analysis was performed on 40 patients(age≥90 years) with OVCFs who underwent PVP between June 2012 and June 2019. There were 7 males and 33 females, age from 90 to 101 years old with an average of (94.6±1.6) years. Patients were divided into two groups according to whether adjacent vertebral refracture occurred after PVP. Among them, 20 patients occurred refracture after PVP (refracture group) and 20 patients did not occur it(control group). The general information, radiological data and pelvic parameters of the two groups were collected. The items included age, gender, body mass index (BMI), fracture site and bone mineral density(BMD) T-value, fracture to operation time, compression degree of injured vertebra, recovery degree of anterior edge of injured vertebra, bone cement injection amount, bone cement leakage, pelvic index(PI), pelvic tilt angle (PT), sacral angle(SS), et al. Factors that may be related to refracture were included in the single-factor study, and multivariate Logistic regression analysis was performed on the risk factors with statistical significance in the single-factor analysis to further clarify the independent risk factors for refracture of adjacent vertebral bodies after PVP. There were no significant differences in age, gender, fracture site, fracture to operation time, compression degree of injured vertebra and recovery degree of anterior edge of injured vertebra between two groups (P>0.05). There were significant differences in BMI, BMD T-value, bone cement injection amount and bone cement leakage rate between two groups(P<0.05). The PI and PT values of the refracture group were higher than those of the control group(P<0.05). There was no significant difference in SS between two groups (P>0.05). Multivariate Logistic regression analysis showed that decreased BMD T-value, bone cement leakage, increased PT and PI values increased the risk of recurrence of adjacent vertebral fractures in OVCFs (P<0.05). There are many risk factors for the recurrence of adjacent vertebral fractures in super-old patients with OVCFs. Patients with high PI and PT values may be one of the risk factors.

Off‐State Drain Leakage Current Mechanism in GaN High Electron Mobility Transistors from Thermal Storage Test

The effect of the thermal storage test on GaN high electron mobility transistor (HEMT) is investigated in this study by observing off‐state drain leakage current and on‐state hysteresis and maximum transconductance. The recovery post thermal stress is also observed which points to native defects within the heterostructure as the leakage values after 72 hrs of recovery are measured to be one order higher than the pristine device. On‐state hysteresis recovery and off‐state drain leakage are dominated by similar defects as observed from the characteristic recovery times. Trap analysis through drain current spectroscopy reveals an additional trap level of EC – 0.83 eV from the thermal storage test. The electrical measurements are in close correlation with the Raman active mode leading to reduced lifetimes (≈0.44 ps) at higher temperatures, where anharmonic phonon decay is observed following the reported Balkanski model. This gives an overall picture of the phonon‐induced defect generation from the thermal stress and the recovery from the reduced scattering probability within the material observed after 72 hrs recovery; also observed within the HEMTs from electrical characterization.

Effect of plasma activated water on Escherichia coli disinfection and quality of kale and spinach

Plasma activated water (PAW) is one of the promising technologies for fresh food disinfection. In this study, PAW was generated by activating water under nonthermal plasma for 10, 20, 30, 45 and 60 min. The effectiveness of Escherichia coli inactivation by PAW treatment on kale and spinach samples was assessed. The differences between kale and spinach samples in terms of the product quality and nutritional characteristics upon PAW treatment was also investigated. Further, changes in leaf structure and surface morphology upon PAW treatment was also evaluated through FTIR cuticle analysis and SEM imaging of leaf surfaces. Results showed that, around 6 log CFU/g reduction in E. coli population was observed in PAW-45 min treatment. However, PAW treatment significantly reduced the total chlorophyll content in both kale and spinach. The total phenolic content, flavonoid content and ascorbic content were altered according to the PAW activation time. Further, kale and spinach behaved differently in terms of antioxidant activity and membrane electrolytic leakage values upon PAW treatment. Clear changes in the cuticular layer and the surface morphological characteristics of the leaf samples were observed after PAW which could be the reason for the significant differences between kale and spinach characteristics in response to PAW treatments.

Comparison of physical and mechanical properties of three different restorative materials in primary teeth: an in vitro study

Physical and mechanical properties of three different (Ketac Molar Easymix, Dyract XP, Cention N (CN)) restoratives with different ingredients were evaluated. Four groups were obtained; Group 1: CN LightCure, Group 2: CN SelfCure, Group 3: Ketac Molar Easymix and Group 4: Dyract XP. Disk-shaped samples (n = 10) were prepared and evaluated for the surface roughness test using a profilometer. For the flexure strength test, 2 × 2 × 25mm bar-shaped samples (n = 10) were prepared, and a three-point bending test was applied to the samples. After preparing cavities for microleakage tests, teeth were restored with restoratives, immersed in dye, and microleakage was assessed. For the microtensile bond strength (µTBS) test, ten sticks were obtained for each group and were stressed under tension. According to surface roughness tests, CN SelfCure showed the lowest value (0.13μm), while Ketac Molar Easymix showed the highest value (0.28μm), and significant differences were found between the groups. In flexural strength tests, the highest values were seen in CN SelfCure (82.94MPa), with statistically significant differences between the groups. When CN SelfCure was applied with an adhesive, the teeth showed statistically decreased leakage than other groups on the gingival side. Higher leakage values were seen on the gingival side than the occlusal side in most groups, and the SelfCure groups showed decreased leakage than the LightCure groups. According to µTBS tests, the highest value was obtained in CN SelfCure-Adhesive group, while the lowest was in CN LightCure-Non-adhesive group. When µTBS was evaluated regardless of adhesive use, the SelfCure groups showed higher µTBS values than the LightCure groups. As a result of the µTBS and microleakage test, the difference between the use of CN with and without adhesive, regardless of the polymerization type, was found to be significant (p < 0.05). Cention N showed better properties in SelfCure mode, compared to the rest materials tested, but further in vitro and in vivo studies are needed to investigate the effect of different polymerization modes and the oral environmental conditions.

Analysis of 7T SRAM Cell Based on MTCMOS, SVL and I-SVL Technique

Objective: To design and propose an optimized Volatile 7T based SRAM cell in terms of leakage currents and dynamic power. Methods: The methodology involved is Multi threshold Voltage CMOS (MTCMOS), Self Controllable Voltage Level (SVL) and Improved Self Controllable Voltage Level (I-SVL). Findings: The proposed work demonstrates that 7T based SRAM cell using I-SVL method is efficient in terms of leakage currents and dynamic power. Also, Comparative Leakage current and dynamic power analyses are done between MTCMOS, SVL, and I-SVL methods The Proposed work based on I-SVL is significant than the MTCMOS and SVL Technique. All the circuits are developed using the Cadence virtuoso tool and spectre simulator is used to carry out the simulation. Novelty: The paper proposes Low power Volatile Memory cell based on 7T with improvements in leakage and dynamic power values in comparison with the earlier literatures. The proposed I-SVL based cell is 89% and 85% efficient in terms of dynamic power in comparison with the earlier references. Keywords: Improved – Self Controllable Voltage Level (I-SVL); Improved Lower SVL (I-LSVL); Improved Upper SVL (I-USVL); Multi threshold Voltage CMOS(MTCMOS); Self Controllable Voltage Level (SVL); SRAM

Genome-Wide Analysis of the WRKY Gene Family in Malus domestica and the Role of MdWRKY70L in Response to Drought and Salt Stresses.

The WRKY transcription factors are unique regulatory proteins in plants, which are important in the stress responses of plants. In this study, 113 WRKY genes were identified from the apple genome GDDH13 and a comprehensive analysis was performed, including chromosome mapping, and phylogenetic, motif and collinearity analysis. MdWRKYs are expressed in different tissues, such as seeds, flowers, stems and leaves. We analyzed seven WRKY proteins in different groups and found that all of them were localized in the nucleus. Among the 113 MdWRKYs, MdWRKY70L was induced by both drought and salt stresses. Overexpression of it in transgenic tobacco plants conferred enhanced stress tolerance to drought and salt. The malondialdehyde content and relative electrolyte leakage values were lower, while the chlorophyll content was higher in transgenic plants than in the wild-type under stressed conditions. In conclusion, this study identified the WRKY members in the apple genome GDDH13, and revealed the function of MdWRKY70L in the response to drought and salt stresses.

The Effect of Combining Post-Harvest Calcium Nanoparticles with a Salicylic Acid Treatment on Cucumber Tissue Breakdown via Enzyme Activity during Shelf Life.

In the present study, an experiment was carried out on the postharvest of cucumber fruit during a 14-day shelf life. The aim was to assess the impact of calcium nanoparticles (CaNPs) blended with different concentrations of salicylic acid (SA) on the shelf life of cucumbers during the seasons of 2018 and 2019. The investigation further monitored the influences of CaNPs-SA on some physical properties of cucumber, including the percentage weight loss, color, and fruit firmness. In addition, chemical properties, such as total soluble solids (SSC%), total acidity (TA%), total soluble sugars, and chlorophyll pigmentation of the fruit skin, were assessed during a 14-day shelf lifeCell wall degradation enzymes (CWEAs) such as polygalacturonase (PG), cel-lulase (CEL), xylanase (XYL), and pectinase (PT) were also researched. In addition, the generation rates of H2O2 and O2•− were calculated, as well as the reduction of DPPH. The lipid peroxidation (malondialdehyde, MDA) and cell membrane permeability (IL%) of cell wall composites were also determined. CaNPs-SA at 2 mM suppressed CWEAs, preserved fruit quality, reduced weight loss throughout the shelf-life period, and reduced the percent leakage value. At this concentration, we also found the lowest levels of MDA and the highest levels of DPPH.

Design, additive manufacturing and component testing of pneumatic rotary vane actuators for lightweight robots

Purpose This study aims to investigate the suitability of a multi-step prototyping strategy for producing pneumatic rotary vane actuators (RVAs) for the development of lightweight robots and actuation systems. Design/methodology/approach RVAs typically have cast aluminum housings and injection-molded seals that consist of hard thermoplastic cores and soft elastomeric overmolds. Using a combination of additive manufacturing (AM), computer numerical control (CNC) machining and elastomer molding, a conventionally manufactured standard RVA was replicated. The standard housing design was modified, and polymeric replicas were obtained by selective laser sintering (SLS) or PolyJet (PJ) printing and subsequent CNC milling. Using laser-sintered molds, actuator seals were replicated by overmolding laser-sintered polyamide cores with silicone (SIL) and polyurethane (PU) elastomers. The replica RVAs were subjected to a series of leakage, friction and durability experiments. Findings The AM-based prototyping strategy described is suitable for producing functional and reliable RVAs for research and product development. In a representative durability experiment, the RVAs in this study endured between 40,000 and 1,000,000 load cycles. Frictional torques were around 0.5 Nm, which is 10% of the theoretical torque at 6 bar and comparable to that of the standard RVA. Models and parameters are provided for describing the velocity-dependent frictional torque. Leakage experiments at 10,000 load cycles and 6 bar differential pressure showed that PJ housings exhibit lower leakage values (6.8 L/min) than laser-sintered housings (15.2 L/min), and PU seals exhibit lower values (8.0 l/min) than SIL seals (14.0 L/min). Combining PU seals with PJ housings led to an initial leakage of 0.4 L/min, which increased to only 1.2 L/min after 10,000 load cycles. Overall, the PU material used was more difficult to process but also more abrasion- and tear-resistant than the SIL elastomer. Research limitations/implications More work is needed to understand individual cause–effect relationships between specific design features and system behavior. Originality/value To date, pneumatic RVAs have been manufactured by large-scale production technologies. The absence of suitable prototyping strategies has limited the available range to fixed sizes and has thus complicated the use of RVAs in research and product development. This paper proves that functional pneumatic RVAs can be produced by using more accessible manufacturing technologies and provides the tools for prototyping of application-specific RVAs.

Electrical property and phase transition analysis of KNN-based lead-free ferroelectric films

The electrical properties, phase construction, thermal stability and phase transition behavior are investigated in 0.95(K0.49Na0.49Li0.02)(Ta0.2Nb0.8)O3–0.05BaZrO3 with 2 wt% MnO2 (KNNLT-BZM) lead-free epitaxial ferroelectric film on LaNiO3 (LNO)-coated SrTiO3 (STO) (001) substrate. The x-ray diffraction results show that a mixed orthorhombic (O) and tetragonal (T) phase is obtained in KNNLT-BZM film. The MnO2 doping can effectively suppress its leakage current, which greatly improves the electrical performance featured by a lower leakage value of 8 × 10–11 A cm−2, a twice remnant polarization of 44.7 μC cm−2, and frequency-dependent ferroelectricity between 50 Hz and 10 kHz. Strikingly, the KNNLT-BZM film maintained ferroelectric nature up to 200 °C, and exhibit a phase transition from O + T mixed phase to T phase (T Mix-T) at 300 °C with a high Curie temperature above 440 °C. These results suggest great application potentials of KNN-based films in lead-free micro-electronic devices.

Comparative evaluation of coronal sealing ability of light cure temporary restorative materials with conventional temporary restorative material using stereomicroscope

Background: The coronal seal is a crucial factor in success of endodontic therapy. Hence the aim of the present study was to assess the sealing ability by evaluating microleakage of three different types of interim restorative materials. Method: A total of 80 extracted human premolars were divided randomly in to 4 groups. Group A: Control group, Group B: Systemp Inlay, Group C: Temp IT Blue, Group D: Cavit-G. Standardized access cavity preparation was done followed by placement of cotton pellet in the access cavity, Interim restorative materials were placed as per the assigned group of restorative materials. Teeth were stained with 2% methylene blue solution for 1 week after which all the teeth were analysed for dye penetration under stereomicroscope. Statistical analysis of data was done using one-way ANOVA and Post Hoc Tukey test with a significance level of P ≤ 0.05. Results: Systemp Inlay showed the least micro leakage value followed by Temp.it blu and Cavit G. Intergroup comparison showed statistically significant difference between Systemp Inlay and other groups whereas Temp IT Blue and Cavit G showed no statistical significance.

MsSAMS, a cold stress-responsive gene, provides resistance to environmental stress in T2-generation transgenic plants

The SAMS (S-adenosylmethionine synthetase) gene is known to play an important role in the mechanism of cold resistance, as overexpression of this gene results in phenotypic changes in T1-generation transgenic plants. Accordingly, this study was conducted to test the expression of the MsSAMS gene in T2-generation transgenic plants and to investigate the resistance of these plants and the function of the transgene in response to various environmental stresses. For the morphological analysis of T2-generation transgenic plants overexpressing the MsSAMS gene, observations using scanning electron microscopy (SEM) were performed. T2-generation transgenic plants were obtained by planting a total of 5 lines, and their characteristics were tested by comparisons with those of the control. SEM revealed that the thickest leaves were produced by the T6 transgenic line-161.24 ± 8.05µm. The number of stomata ranged from 20.00 ± 2.65 to 34.00 ± 1.00 in the T2-generation transgenic plants, but the control had more stomata. Resistance to various factors, such as low temperature, drought, and oxidative stress, in the T2-generation transgenic plants was also confirmed. Under cold-stress conditions, the T6 transgenic line presented the lowest value (22.73%) of ion leakage, and under drought-stress conditions, compared with the control, the transgenic lines presented lower ion leakage after being treated with various concentrations of mannitol. Even under oxidative-stress conditions, the T2-generation transgenic plants presented ion leakage levels that were 32.91 ± 4.24 to 48.33 ± 3.54% lower than those of the control after treatment with various concentrations of methyl viologen. Regarding SAMS enzyme activity, as the duration of cold treatment increased, the activity in the transgenic plants tended to decrease and then increase. During 48h of cold treatment, the control showed a decrease in SAM content, while the T2-generation transgenic plants presented an increase in SAM content, from 13.58 ± 1.04 to 22.75 ± 1.95mg protein/g FW. The results suggest that the MsSAMS gene may be important to the mechanisms of resistance to oxidative and drought stresses in addition to its previously known association with cold resistance. Based on these results, it was suggested that the MsSAMS gene, whose expression is induced by cold stress, can serve as a marker of various responses to environmental stresses, because resistance to cold damage and various environmental stresses are stably inherited in the T2 generation.

Investigation of Groundwater Logging for Possible Changes in Recharge Boundaries and Conditions in the City of Aswan, Egypt

Groundwater is of great importance in our daily life, and its importance is due to its multiple uses, whether in agriculture, industry or other uses. Increasing the Groundwater Levels (GWL) in any area is a great benefit for its importance and multiplicity of uses, but in the city of Aswan, it is different, as the increase in the GWL causes severe damage to buildings and leads to poor quality of agricultural land and the destruction of infrastructure due to the lack of good management. The main objective of this study is to develop a conceptual model of the groundwater system to gain better understanding of water dynamics in the study area and to investigate different management scenarios of the use of groundwater. The model was developed using MODFLOW code to achieve the objective of the study, where the necessary field data were collected to feed the model from the study area, such as Surface Water Levels (SWL) in the Aswan Dam lake and the Nile River, GWL in the Aswan Aquifer and the different characteristics of the layers constituting the aquifer, such as porosity and recharge for different periods to ensure obtaining the most accurate and best results from the model. The model was calibrated with mean residual and absolute mean residual which reached −0.08 and 0.629 m, respectively, with a Root Mean Square Error (RMSE) of 0.737m and a normalized RMSE of 4.319%. Two future scenarios have been developed to arrive at a future vision of GWL in the Aswan aquifer. The first scenario investigated GWL in the study area by changing the values of recharge to the aquifer resulting from an increase in the drinking water and sewage networks’ leakage values, which were predicted in the future for years 2025, 2030, 2035 and 2040. The GWL in the study area are increasing as a result of the increase in the amount of leakage in the years 2025, 2030, 2035 and 2040 compared to the GWL in the study area for the year 2020 by 0.29%, 1.31%, 2.01% and 3.16%, respectively. The second scenario investigated GWL by changing the water levels in El hebs (the lake between the High Dam and the Aswan Dam) as follows (108 m, 110 m, 112 m, 114 m, 116 m and 118 m), where the groundwater levels were calculated in the Aswan Aquifer corresponding to each level. The percentage of increase in groundwater levels corresponding to the levels 108 m, 110 m, 112 m, 114 m, 116 m and 118 m compared to the groundwater levels at the level of 106 m was found as follows: 0.92%, 2%, 2.87%, 4.05%, 4.91% and 5.67%, respectively. The simulation results are intended to support integrated groundwater modeling for the components of the hydrological water budget in the city of Aswan. Furthermore, the model provides us with a better understanding of long-term scenarios for the waterlogging in the city. The results are useful for managing the water logging problems and planning the future infrastructure in the city of Aswan.

Stones hounsfield unit value and predictors of urinary leakage after PCNL

BackgroundTo evaluate the predictive factors of urinary leakage (UL) following percutaneous nephrolithotomy (PNL) and to investigate the relationship between Hounsfield unit (HU) of stone and UL.MethodsWe retrospectively reviewed the data of 728 patients who underwent PNL between January 2012 and January 2017. In total, 396 patients were eligible for the study. Patient demographics, renal factors, stone properties and operative details were collected. The association between UL and these variables were assessed by univariate and multivariate analysis.ResultsThere was no statistically significant correlation considering age, body mass index and the presence of hypertension. The presence of diabetes mellitus (DM) was significantly correlated with UL (p < 0.001). Kidney related factors such as parenchymal thickness, hydronephrosis grade (HN), previous stone treatment, and stone related factors, such as stone surface area, stone burden, stone localization and HU value of stone, were found to affect UL status significantly (p < 0.001). Operation time, fluoroscopy time, treatment outcome, j stent use, percutenous nephrostomy (PCN) catheter stay time and the hospitalization time also had significant effect on UL (p < 0.001). Multivariable logistic analysis showed that presence of DM, parenchymal thickness, HU values, HN, operation time, j stent use, and PCN catheter stay time are independently related with UL following PCNL. Moreover, we determined a HU cut-off value of 933 with ROC analysis, which demostrated 84.9% sensitivity and 67.1% specificity for predicting UL.ConclusionThis study has shown that we are more likely to encounter postoperative UL in stones with higher HU values. Therefore, we suggest clinicians to inform patients with this type of kidney stones about the probable complication of UL.