Abstract Objective The purpose of this study was to investigate the role of language on neurocognitive test outcomes and concussion symptom ratings in professional football players. Methods Design/Setting - A retrospective cross-section analysis of 1546 male Canadian Football League (CFL) athletes was conducted using baseline data collected from the Immediate Post-Concussion Assessment and Cognitive Test (ImPACT) across the 2016–18 competitive seasons. Independent Variables - Participants (1546) were divided into three language categories, native English-speaking, bilingual – whose first language was English, and English as a second language (ESL). Years of education, age, and concussion history were entered as co-variates. Outcome Measure -The 5 Composite scores from ImPACT and the 22 symptoms from the post-concussion symptom scale (PCSS). Results Results of the MANCOVA showed no significant differences between language groups on any of the five ImPACT composite scores F(10, 3072) = 1.09, p = 0.36. The Kruskall-Wallis test revealed significant differences were found in three symptoms including dizziness [X2(2, 1486) = 32.85, p < 0.001)], sadness [X2(2, 1486) = 6.505, p = 0.04], and concentration [X2(2, 1486) = 11.01, p = 0.004)] with the bilingual and non-native English speakers having higher scores. Conclusions This study suggests that cultural and linguistic differences should be considered when administering CNTs. While differences in cognitive outcomes have not been consistently found across studies, differences in baseline symptom reports have been consistently demonstrated. Information pertaining to a patient’s level of acculturation and language proficiency is important for examiners when working with diverse populations. Continuing to develop language-specific normative databases is encouraged.