Leafy Vegetables Research Articles

A review of conditions influencing fate of Shiga toxin-producing Escherichia coli O157:H7 in leafy greens.

The accuracy of predictive microbial models used in quantitative microbial risk assessment (QMRA) relies on the relevancy of conditions influencing growth or inactivation. The continued use of log-linear models in studies remains widespread, despite evidence that they fail to accurately account for biphasic kinetics or include parameters to account for the effect of environmental conditions within the model equation. Although many experimental studies detail conditions of interest, studies that do not do so lead to uncertainty in QMRA modeling because the applicability of the predictive microbial models to the conditions in the risk scenarios is questionable or must be extrapolated. The current study systematically reviewed 65 articles that provided quantitative data and documented the conditions influencing the inactivation or growth of Shiga toxin-producing Escherichia coli (STEC) O157:H7 in leafy greens. The conditions were identified and categorized as environmental, biological, chemical, and/or processing. Our study found that temperature (n=37 studies) and sanitizing and washing procedures (n=12 studies) were the most studied conditions in the farm-to-table continuum of leafy greens. In addition, relative humidity was also established to affect growth and inactivation in more than one stage in the continuum. This study proposes the evaluation of the interactive effects of multiple conditions in processing and storage stages from controlled experiments as they relate to the fate of STEC O157:H7 in leafy greens for future quantitative analysis.

P22-10 Nitrite and nitrate content in leafy vegetables

P22-10 Nitrite and nitrate content in leafy vegetables

Impact of Spent Mushroom Substrate Combined with Hydroponic Leafy Vegetable Roots on Pleurotus citrinopileatus Productivity and Fruit Bodies Biological Properties.

Agricultural activities produce large quantities of organic byproducts and waste rich in lignocellulosic materials, which are not sufficiently utilized. In this study, alternative agricultural waste products, namely, spent mushroom substrate (SMS) from the cultivation of edible Pleurotus ostreatus mushrooms and the roots of leafy vegetables from hydroponic cultivation (HRL), were evaluated for their potential to be used as substrates for the cultivation of Pleurotus citrinopileatus and their effects on the quality, the nutritional value, the chemical properties (lipid, protein, carbohydrate, ash, fatty acid and carbohydrate composition) and the bioactive content (total phenolic compounds and antioxidant activity) of produced mushrooms. SMS and HRL (in different ratios with and without additives) and wheat straw with additives (WS-control) were used. During incubation, the linear growth rate of the mycelium (Kr, mm/day) was measured and used for screening. Mushroom cultivation took place in bags, where several characteristics were examined: earliness (duration between the day of substrate inoculation and the day of first harvest) and biological efficiency (B.E. %, the ratio of the weight of fresh mushrooms produced per dry weight of the substrate × 100). Furthermore, this study aimed to investigate the effect of the protein extract (PE) and carbohydrate extract (CE) of P. citrinopileatus after in vitro digestion (fraction less than 3kDa: PE-DP-3; digestate fraction: CE-D, respectively) on the expression of antioxidant-related genes in the THP-1 cell line. The results showed that mushrooms grown on SMS 50%-HRL 40% had the fastest growth (6.1 mm/d) and the highest protein and lipid contents (34.7% d.w.; 5.1% d.w.). The highest B.E. (73.5%), total carbohydrate (65.7%) and total phenolic compound (60.2 mg GAE/g d.w.) values were recorded on the control substrate. Antioxidant activity was observed in all extracts; the total flavonoid content was low in the samples, and the maximum total triterpene value was detected in SMS 80%-HRL 20% (9.8 mg UA/g d.w.). In all mushrooms, linoleic acid (C18:2) was the main fatty acid (above 60%), and fructose was the dominant individual saccharide. In the investigation of the regulation pathway, NFE2L2 gene expression was upregulated only in the SMS 60%-HRL 40% intervention during incubation with CE-D samples. Additionally, the transcription levels of antioxidant-related genes, SOD1, CAT, HMOX1 and GSR, were increased in the SMS 60-30% intervention. Compared to WS, the alternative substrates are observed to trigger a pathway concerning CE that may resist oxidative stress. This study supports the utilization of agricultural byproducts through sustainable and environmentally friendly practices while simultaneously producing high-value-added products such as mushrooms. Therefore, alternative substrates, particularly those containing HRL, could serve as natural sources of antioxidant potential.

WITHDRAWN: Evaluating Collaborative Emission Reduction Efforts in Urban Road Passenger Transportation: insights from the City Scale

Road passenger transportation contributes significantly to carbon dioxide (CO2) and pollutant emissions, posing challenges for urban sustainability, particularly in rapidly urbanizing cities such as Fuzhou, which was recognized as a global sustainable development city for 2023. This study addresses the research gap by integrating road green factors, which refer to the ecological attributes of roads, such as green space, vegetation, and tree coverage that contribute to improving air quality and reducing pollution, into emission reduction strategies for city-scale road passenger transportation. This novel approach has not been extensively explored in the existing literature. The purpose of this study was to identify a rational pathway for emissions reduction by analyzing the economic, demographic, transportation infrastructure, road green space, and policy factor impacts on emissions. Employing a system dynamics (SD) model, this research evaluates the emissions from road passenger transportation in Fuzhou from 2010 to 2030 under various scenarios, including a baseline scenario (BS), controlled private cars travel demand (CPD), promotion of new energy vehicles (PVE), improvement in energy efficiency (IEE), increase in road capacity (IRC), and improvement in road green level (IRG). The principal results indicate that by optimizing the traffic structure and integrating other emission reduction measures, peak CO2 emissions can be achieved by 2024 under the three scenarios or by 2023 under the four scenarios. Furthermore, significant reductions in pollutants of 84.62% and 86.07% are projected, respectively. The study concluded that the economic benefits of CO2 emissions reductions consistently exceeded those of carbon monoxide (CO), hydrocarbon (HC), and nitrogen oxide (NOx) reductions, highlighting the importance of a holistic approach to emissions reduction strategies. Based on these findings, policy recommendations were proposed to guide the formulation of effective road passenger transportation policies aimed at achieving sustainable urban development.

Development and evaluation of a lutein-fortified yoghurt: Stability assessment, sensory properties and human bioavailability study

Lutein is a carotenoid that humans obtain primarily from green-leafy vegetables. However, due to generally low consumption of these foods, this bioactive is not sufficiently consumed. Therefore, there is growing interest in developing lutein-fortified foods to provide an additional source of lutein supplementation. In this study, we developed a lutein-fortified yoghurt and conducted a 72-hour single-dose bioavailability study in human subjects. Lutein remained stable in the yoghurt over shelf life at 14.6 mg of lutein per 100 g of yoghurt. Sensory evaluation yielded results similar to a lutein-free yoghurt. In the bioavailability study, consuming 100 g of lutein-fortified yoghurt significantly increased the average serum concentration of lutein by 1946.86 ± 1514.72 h μg·L−1 in human subjects, following a typical pharmacokinetic curve of absorption and decline. These findings confirm that our lutein-fortified yoghurt is suitable for commercial use and effectively enhances people’s serum lutein levels.

Vegetable and Fruit Intake and LOX-Index in Japanese Municipal Workers.

Vegetable and fruit intake has been reported to be associated with decreased risk of cardiovascular disease. To date, however, no study has examined the association between vegetable and fruit intake and LOX-index, which reflects the progression of atherosclerosis and is a predictive biomarker of stroke and coronary heart disease. Here, we examined the cross-sectional association between vegetable and fruit intake and LOX-index in Japanese municipal workers. Participants were 338 workers (166 men and 172 women aged 19-71 y) with no history of serious disease who participated in a health and nutrition survey. Vegetable and fruit intake was assessed using a validated brief self-administered diet history questionnaire. LOX-index was calculated by multiplying serum concentrations of the soluble form of lectin-like oxidized LDL receptor-1 by those of LOX-1 ligands containing apolipoprotein B. Multiple regression analysis was used to estimate the geometric mean of LOX-index according to tertile of vegetable and/or fruit intake. Total vegetable and fruit intake was associated with a trend toward decreased LOX-index after adjustment for covariates (p for trend=0.067). In stratified analyses by sex, a significant inverse association between total vegetable and fruit intake and LOX-index was observed in women (p for trend=0.023), whereas such association was not observed in men (p for trend=0.70). None of the intakes of vegetables, fruit, green and yellow vegetables, or other vegetables was associated with LOX-index. Our results suggest that higher intake of total vegetables and fruit is associated with a lower LOX-index in Japanese women.

Exploring risk factors of food loss and waste: A comprehensive framework using root cause analysis tools

Food loss and waste occurring early in the food supply chain leads to increased resource wastage, including land, water, fertilisers, pesticides, energy, and labour. Targeting food loss and waste prevention benefits various aspects such as food security, productivity, economic growth, climate change mitigation, resource conservation, and waste management. Understanding the causes of food loss and waste is crucial for the design of effective solutions and their prioritisation. The purpose of this study was to investigate the risk factors and underlying causes of FLW in leafy vegetables, spanning from agricultural production to retail. To achieve this, the research methodology encompassed a case study conducted among small-scale producers and retailers. A comprehensive approach was adopted by integrating a systematic literature review of global food loss and waste causes and those specific to the Latin American context. This approach was complemented by exploratory research, involving interviews with various stakeholders, coupled with root-cause analysis. A methodology was developed for the creation of a causal map, incorporating the 'Five Whys' technique, the current reality tree, and an interrelationship diagram. The study's findings underscore the critical role of retailer-supplier dynamics in the management and flow of products, identifying a significant research gap in the development of comprehensive strategies for waste reduction. By exploring these dynamics, particularly in the context of take-back agreements and the lack of public policies incentivizing food loss and waste reduction, the research reveals the multifaceted nature of food loss and waste and the necessity for collaborative efforts across the supply chain to mitigate its impacts. The application of the structured methodology provides valuable insights into the root causes and critical risk factors of FLW, offering a foundation for the development of integrated strategies aimed at reducing food loss and waste and advancing towards global sustainability goals.

Greenhouse farming: Hydroponic vertical farming- Internet of Things (IOT) Technologies: An updated review

This review paper of literature highlights the importance of greenhouse urban farming technology, hydroponics, aeroponics, aquaponics, vertical farming and applications of Internet of Things (IOT) technologies. The greenhouse farming is a well known modern agriculture technology for optimal plant growth. Hydroponics, a soilless cultivation technique using nutrient solutions under controlled conditions, is used for growing vegetables, high-value crops, and flowers. Vertical farming is a popular trend in hydroponics that involves stacking multiple layers of plants in a vertical arrangement. This farming method saves space, reduces water usage, and increases yields per square foot of growing area. In comparison to conventional greenhouse cultivation, hydroponics requires less fertilizer, pesticides, and water due to the precise control over their distribution. The term “Internet of Things (IOT)” is a system of interconnected computing devices, sensors, objects, microcontrollers, and cloud servers that can transmit data across a network and control other devices remotely without human intervention. A better management of nutrient solution in hydroponic systems requires optimum pH, electrical conductivity (EC), or ions concentration. The three main greenhouse farming gases (GHGs) emissions in hydroponics hi-tech urban farming are nitrous oxide, methane and carbon dioxide (CO2). To measure the levels of CO2 in hydroponics, the more intermediate to advanced grower can use a CO2 monitoring and controllers system. Hydroponic vertical farming is in infancy in India. Although hydroponic vertical farming units for production of crops like strawberry, lettuce and other leafy vegetables, foliage and flowers are functioning in major metros of India. However, the organized hydroponic vertical farms for production of food crops are not available in India. Therefore, only a few successful hydroponic vertical farms have been built in India mainly due to the initial high price tag on construction and the cost of maintaining them afterwards. Another disadvantage is carbon footprint of hydroponic vertical farming is very high, and discourages its applications.

Establishing the Performance of Next-Generation Amplicon Sequencing for Detection of Giardia duodenalis in Ready-to-Eat Packaged Leafy Greens

Giardia duodenalis is a globally distributed intestinal parasite that commonly infects both humans and animals. G. duodenalis is a species complex, which includes eight assemblages that vary both in genetic structure and host specificity. The prevalence of mixed-assemblage G. duodenalis cysts on food, an understudied infection route for G. duodenalis, remains unknown. In the present study, a method able to detect G. duodenalis mixed-assemblage infections using next-generation amplicon sequencing (NGS) of the beta-giardin gene was applied in combination with the US-FDA’s BAM Chapter 19b protocol for the detection of G. duodenalis from fresh produce to ascertain the limit of detection of G. duodenalis on leafy greens. Ready-to-eat baby Romaine lettuce was inoculated with 5 (n = 5), 20 (n = 10), 100 (n = 10), 200 (n = 10), or 1,000 (n = 10) G. duodenalis cysts of the assemblage B strain H3. Detection of G. duodenalis was successful in 100% of the samples seeded with 1,000, 200, and 100 cysts, in 50% of the samples seeded with 20 cysts, and in none of the samples seeded with 5 cysts. We thus demonstrate robust detection of G. duodenalis on packaged leafy greens using the BAM Chapter 19B method coupled with assemblage-sensitive NGS. This protocol provides a new diagnostic tool useful for both prevalence studies and outbreak investigations involving fresh produce that may assist in better describing the role of G. duodenalis in foodborne illness and in protecting consumers from contaminated fresh produce.

Improved Winter Wheat Yield Estimation by Combining Remote Sensing Data, Machine Learning, and Phenological Metrics

Accurate yield prediction is essential for global food security and effective agricultural management. Traditional empirical statistical models and crop models face significant limitations, including high computational demands and dependency on high-resolution soil and daily weather data, that restrict their scalability across different temporal and spatial scales. Moreover, the lack of sufficient observational data further hinders the broad application of these methods. In this study, building on the SCYM method, we propose an integrated framework that combines crop models and machine learning techniques to optimize crop yield modeling methods and the selection of vegetation indices. We evaluated three commonly used vegetation indices and three widely applied ML techniques. Additionally, we assessed the impact of combining meteorological and phenological variables on yield estimation accuracy. The results indicated that the green chlorophyll vegetation index (GCVI) outperformed the normalized difference vegetation index (NDVI) and enhanced vegetation index (EVI) in linear models, achieving an R2 of 0.31 and an RMSE of 396 kg/ha. Non-linear ML methods, particularly LightGBM, demonstrated superior performance, with an R2 of 0.42 and RMSE of 365 kg/ha for GCVI. The combination of GCVI with meteorological and phenological data provided the best results, with an R2 of 0.60 and an RMSE of 295 kg/ha. Our proposed framework significantly enhances the accuracy and efficiency of winter wheat yield estimation, supporting more effective agricultural management and policymaking.

A formative study of the sociocultural influences on dietary behaviours during pregnancy in rural Bangladesh.

Balanced energy protein supplementation (BEP) is recommended for contexts of high maternal undernutrition by the World Health Organization. Despite recent improvements in undernutrition, Bangladesh remains a context where BEP could help accelerate progress towards nutrition goals. In preparation for an effective trial testing a fortified BEP, a qualitative study was undertaken to better understand sociocultural factors influencing dietary behaviours in pregnancy. Married women of reproductive age (n = 23), their husbands (n = 6) and mothers-in-law (n = 6) were interviewed, and focus group discussions were conducted with women (n = 4). Women had a clear understanding of which nutritious foods are important to consume during pregnancy, including green leafy vegetables, dairy and other animal-source foods. Many explained affordability as a barrier to consuming those foods with the desired frequency. Women acquired information about diet and nutrition in pregnancy from community health workers as well as other women in the community. Most preferred to seek information from their own networks before formal health care providers. Women and husbands generally had positive views about micronutrient supplements, although some mothers-in-law were more hesitant. Some food taboos relating to the consumption of certain foods like duck and pigeon meat persist, mainly stemming from concerns for the unborn child. Opportunities exist to build on existing perceptions of healthy diets, potentially framing food or nutrient supplements as a beneficial 'add-on' to promote a healthy pregnancy. There is a scope to strengthen nutrition counselling, especially for the family members, to dispel myths and misconceptions and promote dietary and other support for pregnant women.

Multi-Regression Modeling of Harvest Income in Smallholder Long Coriander Production: Insights from Battambang, Cambodia

The target of this study is to promote Long Coriander production; the most widely recognized leafy vegetable in Southeast Asia and Cambodia. As a substitute for regular coriander, it is valued for its taste and health benefits. The market price for Long Coriander seeds typically goes up and down depending on the season, similar to other fluctuations observed in other agricultural products. In an effort to fulfill the study's expectations, researchers intend to identify the key factors affecting the profitability of Long Coriander production for smallholders in Battambang, Cambodia. Using multi-regression models, the analysis will determine the statistical significance of specific cost categories and their influence on incomes from both single and annual harvests of Long Coriander. Two regression analyses will be applied: ordinary least squares (OLS) and stepwise regression. Understanding the factors influencing Long Coriander harvest income is crucial for developing targeted strategies to improve the livelihoods of rural producers. With the knowledge gained from this study, smallholders will be able to make strategies that will maximize profitability and maintain the long-term sustainability of Long Coriander production within the context of agriculture. Additionally, the study also defines variables that can be controlled by producers, besides the variables that have a significant effect by chance.

Optimizing vertical farm cultivation of Cichorium spinosum L.: White Light's influence and nutrition management

The objective of this study was to examine the integration of a wild leafy vegetable, Cichorium spinosum L., in vertical farms. This research comprises two experiments focusing on different “white” light products and nutrient solutions. During both experiments, the temperature varied between 25 and 28 °C, relative humidity ranged from 50 to 70 %, carbon dioxide was at 450 ppm, and light intensity was set at 300 μmol m−2 s−1 respectively. In the lighting experiment, the three spectra used had the commercial names Neutral, Full and a SunLike™, and their spectral composition (blue:green:red:far-red) were 14:32:43:10, 16:36:40:8, and 21:34:36:7 respectively. The photoperiod was set to 12 h and the plant density was 50 plants m−2. Results showed no significant impact on agronomical parameters and leaf anatomy. The stomatal length and width decreased as the red:blue ratio of the light sources decreased, being greater in the Neutral treatment (red:blue ratio of 3.1) compared to the Full and SunLike™ (red:blue ratios of 2.5 and 1.7 respectively). Based on these results the preferable “white light” product was the one with the highest efficiency and lowest market price at the time of the experiment. In the nutrient solution experiment, the agronomical and nutritional attributes of stamnagathi plants supplied with a control nutrient solution, “N10-Fe15” were compared to plants cultivated under limited nitrogen, “N4-Fe15” and elevated iron, “N10-Fe48”, EC was 1.5 ds m−1, and pH was 5.6–6.5. The experiment simulated commercial practices by increasing the photoperiod to 15 h and plant density to 100 plants per square meter. The results did not demonstrate significant effect of the nutrient solution differences on the agronomical characteristics except from a decrease in total Kjeldahl nitrogen under limited nitrogen conditions. Notably, leaf tissue phosphorus content increased under elevated iron conditions. The nitrate content remained within safe for consumption thresholds for all treatments. Based on these results, stamnagathi can be integrated in vertical farms under limited nitrogen conditions. Stamnagathi's resilience to elevated iron in the nutrient solution demonstrated its potential for future biofortification experiments.

Utilization of Sustainable Food Gardens (P2L) as an effort to improve household food security in Cijaku Village

Food security is the condition of fulfilling food needs in terms of quantity and quality from household to national levels, and one way to meet these needs is through the application of Sustainable Food Gardens (P2L). Sustainable Food Gardens are crucial for enhancing food self-sufficiency, supporting food consumption diversification by cultivating various crops and raising livestock, optimizing land use in household gardens, and contributing to local economic strengthening through local production. The implementation of P2L enables communities to contribute to environmental sustainability in the agricultural sector. This service is conducted in Cijaku Village, Cijaku District, Lebak Regency, Banten Province. It utilizes qualitative descriptive methods by conducting socialization, assistance, focus group discussions, and evaluation. Empowering communities through Sustainable Food Utilization to support household food security involves various factors that support sustainability and food self-sufficiency at the local level. The results of the socialization showed that 48.14% of respondents did not know about the P2L program before, and 40.74% had never attended related training. After the socialization, 51.85% of the community understood that P2L could help family food security, and 59.26% started implementing P2L by planting green vegetables. Socialization is considered easy to do (48.14%) and well-delivered (51.85%). P2L can support food diversification, and sustainable land management, serve as an adaptation strategy to climate change, optimize water usage, empower women, increase household production, and enhance community involvement in meeting food needs independently.

Comparative Life Cycle Assessment of alternative winter salad value chains supplying the United Kingdom

Lettuce is an established food commodity in the UK increasingly facing supply challenges in winter due to adverse weather events and rising energy costs. We investigate whether an agroecologically grown salad mix of lettuce and underutilised leafy greens produced in the Azores, Portugal, could be part of a sustainable solution. We performed a Life Cycle Assessment to compare the environmental impacts of this salad mix with four other value chains for winter salad supply to the UK: conventional open-field lettuce production chains in Spain using (1) current irrigation practices; (2) 100 % desalinated irrigation water; or hydroponic controlled environment agriculture within the UK powered by (3) the national electricity mix; (4) 100 % wind-generated electricity. Results indicated that the leafy-greens agroecological value chain incurred the smallest environmental burdens across 7 to 11 of 16 impact categories studied. Substituting Spanish winter salad supply with agroecological leafy green production in the Azores, if well managed, could reduce many environmental burdens whilst diversifying leafy greens intake. Nevertheless, all winter value chains were associated with larger environmental burdens than conventional open-field production of lettuce in the UK summer, pointing to the importance of seasonal consumption and wider adoption of agroecological techniques to effectively reduce environmental impact.

Efektifitas pupuk organic cair limbah sajen (limbah sayuran dan tajin) terhadap pertumbuhan tanaman cabai (capsicum annum l)

Background: Consumption green vegetables domestic is so high because of an awareness to start healthy live, it will generate green vegetable waste of a leaf that is not cooked because it is eaten caterpillar or vegetable stale that are not suitable consumption at home. Besides green vegetable waste, in the house also produced waste water from washing rice ( tajin ), basically Indonesian always eats rice, hence waste water from washing rice ( tajin ) will be produced every day. Conclusion: Waste water from washing rice ( tajin ) cause many spent, while green vegetable waste will cause pollution smell to the environment. Solution the both of waste has liquid organik fertilizer is making green vegetable waste and waste water from washing rice that can increase the growth of plants. Methods: A method of writing that is used is carried out the identification of a problem, study of literature, an analysis of the potential, design and strategy. Findings: The treatment aims to understand the organik fertilizers green vegetable waste and waste water from washing rice can increase the higher of plants and the leaves of red chili.

Evaluating the Nutritional Value of Dehydrated Green Leafy Vegetables (Bengal Gram, Fenugreek, and Spinach)

Evaluating the Nutritional Value of Dehydrated Green Leafy Vegetables (Bengal Gram, Fenugreek, and Spinach)

Berry Pomace Extracts as a Natural Washing Aid to Mitigate Enterohaemorrhagic E. coli in Fresh Produce.

Enterohemorrhagic Escherichia coli (EHEC) outbreaks have been frequently linked to the consumption of produce. Furthermore, produce grown on organic farms possess a higher risk, as the farmers avoid antibiotics and chemicals. This study sets out to evaluate the effectiveness of advanced postharvest disinfection processes using berry pomace extracts (BPEs) in reducing EHEC load in two common leafy greens, spinach and lettuce. Spinach and lettuce were inoculated with ~5 log CFU/leaf EHEC EDL-933 and then treated with three different concentrations of BPE (1, 1.5, and 2 gallic acid equivalent, GAE mg/mL) for increasing periods of time. After the wash, the bacteria were quantified. Changes in the relative expression of virulence genes and the genes involved in cell division and replication and response against stress/antibiotics were studied. We observed a significant reduction in EHEC EDL933, ranging from 0.5 to 1.6 log CFU/spinach leaf (p < 0.05) washed with BPE water. A similar trend of reduction, ranging from 0.3 to 1.3 log CFU/mL, was observed in pre-inoculated lettuce washed with BPE water. We also quantified the remaining bacterial population in the residual treatment solutions and found the survived bacterial cells (~3 log CFU/mL) were low despite repeated washing with the same solution. In addition, we evaluated the phenolic concentration in leftover BPE, which did not change significantly, even after multiple uses. Alterations in gene expression levels were observed, with downregulation ranging from 1 to 3 log folds in the genes responsible for the adhesion and virulence of EHEC EDL933 and significant upregulation of genes responsible for survival against stress. All other genes were upregulated, ranging from 2 to 7 log folds, with a dose-dependent decrease in expression. This finding shows the potential of BPE to be used for sanitation of fresh produce as a natural and sustainable approach.

Patterns of food consumption among women with breast cancer: A multicenter study in Indonesia

BackgroundBreast cancer (BC) is a leading cancer among women globally, with a rising incidence in Indonesia. Dietary habits are implicated in BC development and progression, yet there is limited understanding among Indonesian women. This study aims to evaluate the patterns of food consumption among Indonesian women with BC. MethodsA cross-sectional study was conducted among Indonesian women with BC at three national hospitals. The sample size was determined to be 362 participants. Each participant was provided with information and asked to provide written informed consent before face-to-face interviews. Dietary behaviors were assessed using a food frequency questionnaire (FFQ) adapted to local cuisine. Exploratory factor analysis was used to describe dietary behavior patterns. All analyses were performed using R version 4.3.0 with the epicalc and psych packages. ResultsParticipants were predominantly aged 50 years or older, had middle incomes, and exhibited varied dietary habits. We identified three dietary patterns: the “Carnivorous and Sugar” diet, the “Vegetables and Snacks” diet, and the “Traditional” diet. The “Carnivorous and Sugar” diet was characterized by a high consumption of meat, poultry, sweetened drinks or condiments, and desserts. The “Vegetables and Snacks” diet involved more frequent consumption of leafy green vegetables, yellow or orange vegetables, packaged snacks, and unpackaged snacks. The “Traditional” diet was marked by a high intake of tubers, eggs, and soybean products. ConclusionThis study's findings enhance our understanding of dietary patterns among Indonesian women with BC and their potential impact on BC risk. These findings highlight the importance of promoting healthy dietary habits, such as consuming more fruits, and vegetables while reducing the intake of red and processed meats, and sugary foods for BC prevention.

Enhancing Leafy Greens’ Production: Nutrient Film Technique Systems and Automation in Container-Based Vertical Farming

Urban agriculture has emerged as a crucial strategy to address food security and sustainability challenges, particularly in densely populated areas. This study focused on enhancing leafy greens’ production, specifically lettuce (Lactuca sativa L.) and arugula or rocket (Eruca sativa L.), using Nutrient Film Technique (NFT) systems and automation in container-based vertical farming. The study utilized a 20-foot shipping container retrofitted to create a thermally insulated and automated growth environment equipped with energy-efficient LED lighting and precise climate control systems. The results demonstrated significant improvements in crop yields, with the NFT systems achieving productivity up to 11 times higher than traditional methods in protected horticulture. These systems enabled continuous cultivation cycles, responding to the high market demand for fresh local produce. Moreover, the integration of low-cost sensors and automation technologies, each costing under USD 300, ensured that the environmental conditions were consistently optimal, highlighting this approach’s economic feasibility and scalability. This low-cost framework aligns with industry standards for affordable technology, making it accessible for small- to medium-sized urban agriculture enterprises. This study underscores the potential of vertical farming as a sustainable solution for urban food production. It provides a model that can be replicated and scaled to meet the growing demand for healthy, locally grown vegetables.