Leaf Supplementation Research Articles

Impact of Maternal Moringa oleifera Leaf Supplementation on Milk and Serum Vitamin A and Carotenoid Concentrations in a Cohort of Breastfeeding Kenyan Women and Their Infants.

Background: Childhood vitamin A deficiency leads to increased morbidity and mortality. Human milk is the only source of vitamin A for exclusively breastfed infants. Dried Moringa oleifera leaf powder (moringa) is a good food source of provitamin A and other carotenoids. Its effect during lactation on human milk vitamin A and carotenoid content is unclear. Objectives: Our objective was to investigate the effect of maternal moringa consumption on human milk retinol and carotenoid concentrations and maternal and infant vitamin A status. Methods: We conducted a 3-month pilot single-blinded cluster-randomized controlled trial in breastfeeding mother-infant pairs (n = 50) in Kenya. Mothers received corn porridge with (20 g/d) or without moringa with complete breast expressions and maternal and infant serum collected at enrollment (infant <30 days old) and 3 months. Milk was analyzed for retinol and selected carotenoids; maternal/infant serum was analyzed for retinol binding protein (RBP). Results: 88% (n = 44) pairs completed milk and serum samples. Four mothers (9%) had vitamin A deficiency (RBP <0.07 µmol/L); 11 (25%) were vitamin A insufficient (VAI; RBP <1.05 µmol/L). Alpha-carotene concentration in milk was higher in the moringa than the control group at baseline (p = 0.024) and at exit (least squares means, LSM, 95%CI µg/mL 0.003, 0.003-0.004 moringa vs. 0.002, 0.001-0.003 control, n = 22/cluster; p = 0.014). In mothers with VAI, alpha-carotene was higher in the moringa group than controls at exit (LSM, 95%CI µg/mL 0.005, 0.003-0.009 moringa, n = 3, vs. 0.002, 0.000-0.004 control, n = 8, p = 0.027) with no difference at baseline. Milk carotenoids did not correlate with vitamin A status (serum RBP) in infants or mothers. Conclusions: Maternal moringa consumption did not impact concentration of milk vitamin A and resulted in limited increase in milk carotenoids in this cohort.

Economic Value of Bamboo Leaf on Nutrition Intake, Productivity, and Growth Performance on Boer Goat

This study investigated the effects of bamboo leaf supplementation on nutrition intake, productivity, and growth performance in Boer goats (Capra aegagrus hircus). A randomized complete block design was employed, with four treatment groups: Control (no bamboo leaf supplementation) and three experimental groups receiving Low Dose (50g/day/goat), Medium Dose (100g/day/goat), and High Dose (150g/day/goat) of bamboo leaves. Each treatment group comprised three repetitions. Thirty-six healthy Boer goats of similar age and weight were selected and housed individually with ad libitum access to water and a basal diet. Bamboo leaf supplementation was administered daily for 60 days, following which nutrition intake, productivity, and growth performance were assessed. Results revealed a dose-dependent increase in nutrition intake with higher levels of bamboo leaf supplementation, indicating improved feed consumption in supplemented groups. Productivity, measured as total weight gain per hectare, exhibited a similar trend, with the Medium and High Dose groups demonstrating higher productivity compared to the Control and Low Dose groups. Analysis of growth performance, represented by average daily gain (ADG), further supported these findings, showing higher ADG in the Medium and High Dose groups. Bamboo leaf supplementation positively influenced nutrition intake, productivity, and growth performance in Boer goats. These results suggest the potential of bamboo leaves as a valuable dietary supplement for enhancing goat farming efficiency and livelihoods, particularly in regions where bamboo is abundant.

Effect of Bidens tripartita leaf supplementation on the organism of rats fed a hypercaloric diet high in fat and fructose

Herbs play an important role in folk medicine, and scientific research has confirmed the properties of their use as an alternative treatment, including the treatment and mild correction of metabolic disorders during disease. Trifid bur-marigold (Bidens tripartita) is a pharmacopoeial herbal raw material that is widely used in clinical practice as an external remedy for skin lesions and as an internal remedy for digestive and respiratory disorders. In this work, the general effect of dried leaves of B. tripartita on physiological activity and metabolic processes in model animals on a high-calorie diet was determined. For the experiment, three groups of 18 male white laboratory rats were formed and fed a hypercaloric diet (increased fat content and 20% fructose solution instead of water) for 27 days, in addition to 0.4% and 4.0% dried leaves of B. tripartita. The consumption of 0.4% and 4.0% of the medicinal plant resulted in a significant delay in the body weight gain and the average daily weight gain of the rats compared to the control group. Dried leaves of B. tripartita in the diet of rats decreased the relative weight of the thymus and increased the relative weight of the brain, and at a dose of 4.0%, increased the relative weight of the lungs and individual large intestines (cecum and colon). Dietary supplementation with B. tripartita caused an increase in globulin concentration and changes in protein coefficient. Blood parameters such as: urea, urea nitrogen, inorganic phosphorus, glucose and bilirubin levels changed depending on the dose. In the general blood test, consumption of dried leaves of B. tripartita caused a decrease in hematocrit, hemoglobin concentration and platelet count, but increased the number of eosinophils. Bidens tripartita at both concentrations significantly increased ALT activity with a corresponding change in the blood De Ritis ratio. The addition of 20 g of B. tripartita leaves to the diet increased alkaline phosphatase activity and decreased alpha-amylase activity, while 200 g increased blood gamma-glutamyltransferase activity. At the end of the experiment, the rats' orientation activity, determined in the open field, changed according to the herb dose consumed: 0.4% leaves caused an increase and 4.0% a decrease. Physical activity was reduced and emotional state increased, regardless of the dose of dried B. tripartita leaves, compared to the control group of animals. The results obtained show that the addition of B. tripartita dried leaves as a dietary supplement to a high-calorie diet is safe, does not cause pathological changes or side effects, and has a significant effect on metabolic processes. This provides theoretical support for the use of B. tripartita dried leaves in the manufacture of nutraceutical and pharmacological products for the correction of metabolic disorders in humans and animals. The doses and duration of their application require further studies.

The effect of dietary supplementation of Lycium barbarum leaves on the growth performance, organ indexes and intestinal microflora of rats.

This study was conducted to investigate both fruit and different levels of leaf supplementation on the growth performance, organ indices and intestinal microflora of rats. Twenty-five healthy male Sprague-Dawley rats were randomly divided into five groups. The rats in the control (NC) and positive control (PC) groups were fed by gavage a basal diet and a basal diet with 4 g/kg of L. barbarum fruit homogenate, respectively. The test (LD, MD, and HD) groups were fed basal diets with additional 2, 4, and 8 g/kg of L. barbarum leaf homogenate, respectively. The feeding period was 35 d. The result revealed that the rats in the LD group had the highest average weight gain (p < 0.05). The cardiac and renal indexes in the LD and MD groups were significantly higher than in NC group, respectively (p < 0.05). Diversity analysis revealed that adding low concentrations of L. barbarum leaf homogenates markedly reduced the Shannon index of the rats cecum (p < 0.05). The relative abundance of Verrucomicrobiota was higher in the LD group than those in other groups (p < 0.05). The relative abundance of Actinobacteriota was found significantly higher in PC group than others (p < 0.05). The relative abundance of Akkermansia in LD group was the highest (p < 0.05). The relative abundance of Romboutsia in the PC group was considerably higher than that in other groups. The relative abundance of Candidatus_Saccharimonas in the supplementation groups was appreciably lower than those found in other groups. The relative abundance of Alloprevotella was significantly lower in PC, LD, and MD groups than in NC and HD groups (p < 0.05). The relative abundance of Oscillibacter was significantly higher in HD group than in other groups (p < 0.05). Thus, L. barbarum leaf homogenate fed to rats could increase their growth performance, internal organ weights and additionally enhance the relative abundance of beneficial bacteria. Therefore, based on the obtained data in the current study, a dose of L. barbarum leaf homogenate supplemented with 2 g/kg in diet is recommended, however, further studies are required to confirm, especially in animals.

Influence of dietary mulberry (Morus alba L.) leaf supplementation on production performance, blood metabolites, rumen fermentation characteristics and ruminal bacteria community in lactating dairy cows

The purpose of this study was to determine the effect of mulberry (Morus alba L.) leaf (ML) supplementation on the productivity, blood metabolism, rumen fermentation characteristics and rumen microbial population in lactating dairy cows. Twelve healthy multiparous Holstein dairy cows (initial characteristics: body weight = 442.83 ± 86.87 kg; milk yield = 12.55 ± 1.91 kg/d; days in milk = 150.0 ± 47.0 d) were randomly allocated to two treatment groups of six cows each. The cows were fed two different diets: a control diet (basal diet without ML; CTRL group) and a ML-supplemented diet (basal diet + 300 g ML; ML group). The trial lasted for a total of 28 d after an adjustment period of 14 d. The total rumen volatile fatty acid (VFA) levels (p = .063) and the propionate level (p < .05) were increased in the ML group, whereas the ratio of acetate to propionate was significantly decreased (p < .05). A microbiome study demonstrated that the dietary treatments significantly influenced the composition of rumen bacteria. The ruminal populations of Paludibacter propionicigenes, Ruminococcus sp. JE7A12 and Prevotella oris were greater (p < .05) in ML group than in CTRL group, while Enterococcus faecalis numbers significantly decreased (p < .05) in the ML group. Supplementing a dairy cow diet with 300 g ML has the potential to alter the microbial community and fermentation in the rumen, leading to an increase in propionate synthesis.

Kandungan Antioksidan pada Suplemen Daun Kelor terhadap Kadar Acyl Ghrelin pada Subjek Obesitas

This study aims to explore the effect of antioxidant content on acyl ghrelin levels in obese subjects. This research method is a systematic literature review approach by searching for journal articles in the PubMed database. Inclusion criteria were articles published in the last 10 years using RCT methods on experimental animals and humans with obese nutritional status and providing Moringa leaf intervention. The research results showed that of 69 articles identified, only 7 articles met the inclusion criteria. The findings obtained indicate that the high antioxidant content of Moringa leaves has great potential as a functional food source and is beneficial for body health. The conclusion of the research is that the antioxidant content in Moringa leaves has an effect on obesity and still needs to be studied for its effect on acyl ghrelin. Keywords: Acyl ghrelin, Moringa Leaf Supplements, Antioxidants, Obesity

Mulberry Leaf Dietary Supplementation Can Improve the Lipo-Nutritional Quality of Pork and Regulate Gut Microbiota in Pigs: A Comprehensive Multi-Omics Analysis.

Mulberry leaves, a common traditional Chinese medicine, represent a potential nutritional strategy to improve the fat profile, also known as the lipo-nutrition, of pork. However, the effects of mulberry leaves on pork lipo-nutrition and the microorganisms and metabolites in the porcine gut remain unclear. In this study, multi-omics analysis was employed in a Yuxi black pig animal model to explore the possible regulatory mechanism of mulberry leaves on pork quality. Sixty Yuxi black pigs were divided into two groups: the control group (n = 15) was fed a standard diet, and the experimental group (n = 45) was fed a diet supplemented with 8% mulberry leaves. Experiments were performed in three replicates (n = 15 per replicate); the two diets were ensured to be nutritionally balanced, and the feeding period was 120 days. The results showed that pigs receiving the diet supplemented with mulberry leaves had significantly reduced backfat thickness (p < 0.05) and increased intramuscular fat (IMF) content (p < 0.05) compared with pigs receiving the standard diet. Lipidomics analysis showed that mulberry leaves improved the lipid profile composition and increased the proportion of triglycerides (TGs). Interestingly, the IMF content was positively correlated with acyl C18:2 and negatively correlated with C18:1 of differential TGs. In addition, the cecal microbiological analysis showed that mulberry leaves could increase the abundance of bacteria such as UCG-005, Muribaculaceae_norank, Prevotellaceae_NK3B31_group, and Limosilactobacillus. Simultaneously, the relative levels of L-tyrosine-ethyl ester, oleic acid methyl ester, 21-deoxycortisol, N-acetyldihydrosphingosine, and mulberrin were increased. Furthermore, we found that mulberry leaf supplementation significantly increased the mRNA expression of lipoprotein lipase, fatty acid-binding protein 4, and peroxisome proliferators-activated receptor γ in muscle (p < 0.01). Mulberry leaf supplementation significantly increased the mRNA expression of diacylglycerol acyltransferase 1 (p < 0.05) while significantly decreasing the expression of acetyl CoA carboxylase in backfat (p < 0.05). Furthermore, mulberry leaf supplementation significantly upregulated the mRNA expression of hormone-sensitive triglyceride lipase and peroxisome proliferator-activated receptor α (p < 0.05) in backfat. In addition, mulberry leaf supplementation led to increased serum leptin and adiponectin (p < 0.01). Collectively, this omic profile is consistent with an increased ratio of IMF to backfat in the pig model.

Neem Leaf Supplement Ameliorates Depressive Like Behaviour in Alzheimer’s Disease Model in Adult Male Wistar Rats

Aim of the Study: Currently, reports linked neuropathological changes in Alzheimer’s Disease (AD) to be a risk factor in depression. There is a need to develop natural therapeutics, with strong antioxidant property like Neem leaf, to avert AD’s neuropathological and behavioural changes using animal model induced by neurotoxin aluminium chloride. This study is aimed at ascertaining depressive like disorders in AD model while evaluating mechanism through which Neem averts AD neurodegeneration in the fronto-cerebellar cortex and possible depressive like behaviour. Methodology: Twenty (20) adult male Wistar rats used were grouped (n=5) viz: control group (A), neurodegenerative model given aluminium (B), 200mg/kg Neem leaf supplement (C) and 200mg/kg Neem leaf treated AD model (D). Neurobehavioural changes for reward memory and depressive like behaviour were evaluated using Y-maze (open arm reward test) and the tail suspension test (TST). The frontal and cerebellar cortices were excised, fixed and processed for Haematoxylin and Eosin stain (H and E), Cresyl fast violet (CFV) stain for Nissl bodies and astrocytes immunohistochemistry using glial fibrillary acidic protein (GFAP). Behavioural test data were analyzed using ANOVA and test for significance done @ p&lt;0.05. Results: Aluminum induced neurodegeneration similar to AD pathology characterized by loss of neurons, chromatolysis, proliferation of astrocytes and decline in cognitive function in addition to exhibiting depressive like behaviour seen in a decrease number of reward arm entries, increase in time spent to reach reward arm and immobility time in the TST attributed to loss of cognitive function relative to loss of neurons integrity in the fronto-cerebellar cortex. However, neem leaf supplementation mitigates against AD model neuropathological and neurobehavioural presentations resulting in an improved neurocognition, neuron survival or repair, decline in astrocytes proliferation and decline in depressive like behavior as compared to Aluminum induced AD model. Conclusion: Neem leaf alleviates depressive like behaviour associated with neurocognitive impairment through the interaction between neuron-astrocytes to protect neurons against aluminum induced neuroinflamamation and strengthens neural circuit for cognitive function.

The Impact of Dietary Moringa oleifera Leaf Supplementation on Stress Markers, Immune Responses, and Productivity in Heat-Stressed Broilers

The Impact of Dietary Moringa oleifera Leaf Supplementation on Stress Markers, Immune Responses, and Productivity in Heat-Stressed Broilers

Effect of Moringa olifera leaves on growth and gut microbiota of Nile tilapia (Oreochromis niloticus).

The study was conducted to evaluate the effect of Moringa olifera on the growth and gut health of Tilapia (Oreochromis niloticus). The feed having 30% crude protein was prepared as an experimental diet with 4%, 8% and 10% M. olifera leaf supplementation, respectively. The control diet was devoid of M. olifera leaves. The 10 weeks feeding trial was carried out on 60 fish in aquaria. Fish was fed @ 3% of body weight twice a day. Diet with the high level of inclusion of M. olifera leaves significantly increased the growth rate, Survival Rate (SR), Specific Growth Rate (SGR) and Feed Conversion Efficiency (FCE) in all treatment groups compared to the control group. Similarly, Feed Conversion Ratio (FCR) gradually decreased and found highly-significant. To check the gut health of the Tilapia, random samples were selected and dissected. Nutrient agar was used as culture media to check the growth of bacteria. Pour Plate Method was used for viable colonies count by colony counter. Through staining method, the different bacteria such as Escherichia coli, Salmonella, Shigella and Pseudomonas aeruginosa were identify abundantly in the intestine of control diet fish but less number present in treatment diets groups. These results showed that M. olifera leaves up to 10% of dietary protein can be used for Nile tilapia for significant growth and healthy gut microbiota of fish.

Synergistic antioxidant and anti-inflammatory effects of combined supplementation with oral zinc and dietary Moringa oleifera leaf in streptozotocin-induced diabetic rats

Individual interventions with zinc or M. oleifera leaf powder have shown some promise in managing diabetes-induced oxidative stress and inflammation. This study examined the synergistic effect of oral zinc and dietary M. oleifera leaf supplementation on the serum antioxidant and anti-inflammatory activities in streptozotocin-induced diabetic rats. Seven groups of 56 rats were used in the experiment: (1) normal rats + normal diet, (2) normal rats + 3% M. oleifera leaf-supplemented diet, (3) non-treated diabetic rats, (4) diabetic rats + 200 mg/kg metformin, (5) diabetic rats + 3% M. oleifera leaf-supplemented diet, (6) diabetic rats + 100 mg/kg oral zinc sulfate, and (7) diabetic rats + 3% M. oleifera leaf-supplemented diet + 100 mg/kg oral zinc sulfate. ELISA assay kits were used for antioxidant and anti-inflammatory markers respectively. The results suggest that when all treated diabetic groups were compared with non-treated diabetic control at p&lt;0.05, only groups 4 and 7 showed a significant increase in superoxide dismutase activity. Only group 5 showed a significant increase in reduced glutathione. No significant changes were observed in catalase and the total antioxidant capacity. MDA decreased significantly in group 7 only. There was a significant reduction in interleukin-6 in groups 6 and 7. Only group 7 showed significantly reduced C-reactive protein. Tumor necrosis factor-α reduced significantly in group 6 only. Overall, the combined supplementation with zinc and M. oleifera outperformed the individual treatments with either zinc or M. oleifera in ameliorating diabetes-induced oxidative stress and inflammation, indicating a possible synergistic effect of their combination.

The Effects of Fresh Hemp Leaf Supplementation (Cannabis sativa) on the Physiological and Carcass Characteristics and Meat Quality in Transported Goats.

Transportation stress adversely affects animal health, productivity, and meat quality. Bioactive plant compounds may alleviate transit stress in livestock. This study evaluated the effects of fresh hemp leaf supplementation on blood metabolites, performance, carcass traits, and meat quality in transported goats. Twenty male goats (15 ± 2.76 kgBW) were investigated, some were given a hemp supplement (n = 10) and the remaining goats were used as a control group (n = 10). The hemp group received 10 g/30 kg body weight of fresh leaves prior to transportation. Blood samples were analyzed before and after the 200 km journey. The goats were slaughtered after transit and the quality of the meat examined. In the controlled group, transportation increased neutrophils and electrolytes, but decreased lymphocytes and hemoglobin. In contrast in the hemp-supplemented group, the hemp maintained this animal blood parameters. Body weight and carcass yield, however, did not differ between the two groups. Hemp reduced meat redness at 1 h postmortem but had minimal effects on its pH, color, water holding capacity, tenderness, and texture after 24 h. However, hemp supplementation did alter the odor profiles between the two groups detected by electronic nose sensors. In conclusion, fresh hemp leaf supplementation maintained blood metabolites and had minor advantageous effects on meat quality in response to transportation stress in goats. Further investigation using hemp supplements shows potential to alleviate transit stress, although higher doses may be required in order to further enhance its benefits.

Effect of Eriobotrya japonica Leaf Supplements on Allergic Rhinitis Symptoms and Skin Conditions in Healthy Adults: A Randomized, Double-Blind, Placebo-Controlled Study

Eriobotrya japonica (E. japonica) leaves have been used as an herbal traditional medicine in China and Japan owing to their anti-inflammatory and protective effects against skin conditions and allergy symptoms. These beneficial effects are likely mediated by the various triterpenoids present in E. japonica leaves. However, the efficacy of E. japonica leaves in the treatment of allergic rhinitis has not been evaluated in humans. Therefore, in the present study, a randomized, controlled, double-blind trial was performed on healthy adults of age &gt;20 (n = 27) who were randomly assigned to receive either 2.5 g of placebo or E. japonica leaf supplements once daily for 4 weeks. The Japanese Allergic Rhinitis Quality of Life Standard Questionnaire (JRQLQ), dermatological allergy symptoms, Dermatology Life Quality Index, and skin condition parameters were assessed at baseline and after 4 weeks. Significant differences were observed in the variability of the itchy nose, itchy eyes, and eye symptoms between the E. japonica supplementation and placebo groups after 4 weeks. Arm skin transepidermal water loss was improved only in the E. japonica supplementation group. This study suggests that E. japonica leaves can be used as a functional food ingredient to relieve allergic symptoms.

Mulberry leaf supplementation inhibits skatole deposition by regulating gut microbiota and upregulating liver cytochrome P450 1A1 expression in finishing pigs

Skatole, a strong fecal odor substance, is generated through microbial degradation of tryptophan in the animal hindgut. It easily accumulates in adipose tissue and affects meat quality. In this study, the effect of mulberry leaf supplementation on skatole in finishing pigs was studied. In a 35-day trial, 20 finishing pigs (barrows and gilts) were fed with a basal diet or basal diet with 6% mulberry leaves. Growth performance of the pigs (n = 10) was automatically recorded by a performance-testing feeder system and 8 pigs in each treatment were slaughtered and sampled for the remaining tests. Skatole and short-chain fatty acids were detected using HPLC and gas chromatography, respectively. Fecal microbiota were analyzed using 16S rRNA gene sequencing. The metabolomics analysis of feces and serum was performed with UHPLC-MS/MS. The major cytochrome P450 (CYP) enzymes that catalyze skatole degradation in the liver were tested by using RT-PCR and Western blot. Effects of major bioactive compounds in mulberry leaves on the CYP genes were verified in the hepatic cell line HepG2 in an in vitro test (n = 3). In finishing pigs, mulberry leaf supplementation had no significant effect on the average daily gain, average daily feed intake, and feed conversion ratio (P > 0.05), but reduced skatole levels in feces, serum, and backfat (P < 0.05), and increased acetic acid levels in feces (P = 0.027). Mulberry leaf supplementation decreased the relative abundance of the skatole-producing bacteria Megasphaera and Olsenella (P < 0.05). Indole-3-acetic acid, the intermediate that is essential for skatole production, was significantly reduced in feces by mulberry leaf supplementation (P < 0.05) and was positively correlated with skatole content in feces (P = 0.004). In pigs treated with mulberry leaves, liver CYP1A1 expression was increased (P < 0.05) and was negatively correlated with skatole content in backfat (P = 0.045). The in vitro test demonstrated that mulberry leaf polyphenols and polysaccharides could directly stimulate CYP1A1 expression in hepatic cells. These findings suggest that mulberry leaf supplementation reduces skatole production and deposition in finishing pigs by regulating the gut microbiota and promoting skatole degradation in liver.

Toward smallholder bamboo-integrated agro-silvopastoral systems in sub-Saharan Africa: assessing the impact of bamboo leaves on consumption pattern, growth performance and manure characteristics of West African dwarf goats

IntroductionThe selection of appropriate plant species, particularly those tailored to supplement ruminants raised under agrosilvopastoral systems, remains a persistent challenge.ObjectiveIn a 30-day study, the feed intake, growth performance, and manure characteristics of West African dwarf (WAD) goats fed bamboo leaf supplement were evaluated. Materials and methodsA total of 30 animals with an average weight of 9.9 ± 0.21 kg were divided into a control group, and four bamboo-supplemented groups, following a 2 x 2 factorial arrangement within a completely randomized design. Each group had six animal replicates and received the following dietary treatments: T1 (control) – 400g of basal diet composed of 60% Megathyrsus maximus and 40% Bridelia ferruginea leaves per day; T2—basal diet composed of the T1 diet supplemented with 400 g of Oxytenanthera abyssinica (A. Rich.) Munro leaves per day; T3—basal diet composed of the T1 diet supplemented with 400 g of Bambusa balcooa (Beema) leaves per day; T4—basal diet composed of the T1 diet supplemented with 600 g of O. abyssinica (A. Rich.) leaves per day; and T5—basal diet composed of the T1 diet supplemented with 600 g of B. balcooa leaves per day. The data were analyzed using generalized linear model procedures in Minitab Statistical Software at a 5% significance level. ResultsThe results showed varied (p &amp;lt; 0.05) dry matter compositions spanning from 894.3 to 910 g/kg with the highest contents of crude protein (194 g/kg), nitrogen (31 g/kg), crude fibre (302 g/kg), ash (156 g/kg), acid detergent fibre (429 g/kg), and acid detergent lignin (3.6 g/kg) recorded for B. ferruginea. The leaves of Bambusa balcooa contained the highest neutral detergent fibre (481 g/kg) while M. maximus had the highest organic matter content (911 g/kg). Significant interaction effect (p &amp;lt; 0.05) was observed between the supplement type and the supplementation level, impacting various parameters including feed intake ranging from 398 g (T1) to 469 g (T3) per day, weight gain from 27 g (T1) to 72 g (T2) per day, feed conversion efficiency from 6% (T1) to 15% (T2), manure output from 241 g (T2) to 260 g (T5) per day, carbon- nitrogen (C: N) ratio from 9 (T2) to 20 (T5), faecal acidity levels from 4 (T1) to 5 (T4). Comparatively, higher daily dry matter intake was recorded for the animal groups fed the B. balcooa supplement than their counterparts fed the O. abyssinica supplement. However, the O. abyssinica supplemented groups were more efficient in converting feed to body weight, with feed conversion efficiency approximately 9% higher than the B. balcooa-supplemented groups. Furthermore, in general, the animal groups that received a daily supplement of 400 g gave higher feed efficiency, with performance approximately 3.2% higher compared to the groups receiving 600 g. Likewise, these groups displayed higher faecal N output while concurrently achieving a reduced C: N ratio, indicative of enhanced nutrient utilization and potential environmental benefits. ConclusionTo conclude, integrating 400 grams of bamboo leaves into animal diet can enhance basal diet consumption, accelerate animal growth, and yield manure akin to chemical fertilizers, making it a valuable strategy for bamboo-integrated agro-silvopastoral systems practitioners.

THE PERFORMANCE OF PACIFIC WHITE SHRIMP INFECTED BY Vibrio harveyi AFTER MANGROVE LEAF EXTRACT SUPPLEMENTATION

&lt;p&gt;This research investigated the advantages and determined the optimal dosage of mangrove leaf supplementation to enhance growth and disease resistance in shrimp. PL-20 Pacific White Shrimp were given different doses of mangrove leaf extract added to their feed: 0 ppm/500 g (A), 225 ppm/500 g (B), 250 ppm/500 g (C), and 275 ppm/ 500 g (D), over a 30-day period. This experimental research was conducted using a completely randomized design with four treatments and three replications each. Several parameters were observed: growth, feed conversion ratio (FCR), energy protein productivity (EPP), and subsequent exposure to &lt;em&gt;Vibrio harveyi &lt;/em&gt;to assess survival rate (SR). The obtained data underwent ANOVA analysis, which results demonstrated significant influence in shrimp growth, with the most notable improvement observed in treatment D. Treatment D also exhibited the highest SR value following the challenge test. Throughout the study, water quality parameters remained within the suitable range for fostering shrimp growth&lt;/p&gt;

Effects of Eucommia ulmoides Oliver leaves on gut microbiota and intestinal function during in vitro simulated digestion and fermentation

In this study, the effects of Eucommia ulmoides Oliver (EUO) leaves, both young and mature, on intestinal health were evaluated using in vitro simulated digestion and fermentation model. During the digestion phase, we observed that digestive enzymes gradually released polyphenols from the EUO leaves. Notably, the digestion products of EUO young leaves (EUO-YL) had higher polyphenol content and antioxidant activity compared to those of EUO mature leaves (EUO-ML). In the fermentation stage, the digestion products of EUO leaves effectively modulated the gut microbiota by increasing the relative abundances of short-chain fatty acids (SCFAs) producing bacteria, leading to higher SCFAs concentrations in the EUO leaf supplement groups. Additionally, the fermentation products of EUO leaves improved intestinal barrier function and decreased pro-inflammatory cytokine levels in Caco-2/THP1 co-cultures exposed to lipopolysaccharide (LPS). Our research supports the potential of EUO leaves as a functional food source for the prevention or treatment of related diseases.

Effect of Moringa leaf supplements during pregnancy: Follow-up study on the development of pre-school children in rural area, Indonesia

This study is a follow-up study from experimental research that provides moringa supplements in the form of flour (GTK) and extract (GEK) as well as iron folate supplements (GBF) to pregnant women. Micronutrients are very important for the development of the child's brain during the womb and infancy. This study seeks to see the extent of the effect of Moringa leaf supplementation during pregnant women on the development of children at preschool age (5-6 years) in Jeneponto Regency. This study is an analytical observational study with a cross-sectional design with a total sample of 301 preschool-age children (5-6 years) taken by purposive sampling.in six sub-districts in Jeneponto Regency who when pregnant their mothers received moringa and iron folate supplements.

Dietary Morus alba L. leaf supplementation improves hepatic lipid accumulation of laying hens via downregulating CircACACA

Fatty liver hemorrhagic syndrome (FLHS) is the most common metabolic disease in laying hens. Morus alba L. (mulberry) leaf has the effect of regulating lipid metabolism. We evaluated the effects of dietary 3% mulberry leaf (MUL) supplementation in production performance, egg quality, and liver lipid deposition in laying hens. Differentially expressed genes and circRNAs in the liver were identified using whole-transcriptome sequencing. We also evaluated the effects of the MUL extract using an in vitro model of primary hepatocytes induced by free fatty acids and explored the role of key circRNAs in this process. Dietary supplementation with 3% MUL alleviated liver steatosis in laying hens, as shown by decreased fatty liver color score, relative liver weight (P < 0.01), and triglyceride levels (P < 0.05), and showed a tendency to reduce the mortality rate of laying hens (P=0.09). In addition, mulberry leaf supplementation significantly reduced cholesterol content in egg yolk (P < 0.01). Dietary mulberry leaf supplementation downregulated the expression of genes involved in fatty acid and cholesterol biosynthesis, and upregulated the expression of fatty acid oxidation-related genes in the liver. CircACACA, which is derived from exons 2 and 3 of the acetyl-CoA carboxylase alpha (ACACA) pre-mRNA, was significantly reduced in the MUL group (P < 0.01). Upregulation of circACACA expression reversed the lipid-lowering effect of mulberry leaf extract by upregulating sterol regulatory element-binding proteins 1 c (SREBP-1c) and fatty acid synthase (FASN) (P < 0.05). Overall, mulberry leaf is an effective therapeutic strategy for FLHS in hens and can improve liver lipid metabolism by downregulating circACACA.

Shimanami Leaf Intake Affects Bowel Movement and Intestinal Microbiota in Mice.

The Shimanami Leaf®, produced at Innoshima Island in Onomichi City, Hiroshima Prefecture, Japan, is a leafy vegetable that does not require pesticide use and has a high nutritional value. Although the leaf has abundant dietary fiber and other nutrients, reports on its biological regulatory functions are lacking. Therefore, this study aimed to elucidate the effects of Shimanami leaf intake on bowel movement and gut microbiota in mice. We examined the effects of Shimanami leaves on fecal weight, fecal water content, and intestinal microbiota composition. On day 10 of administration, the Shimanami leaf-treated group exhibited significantly higher fecal weight and water content than the control group. Next-generation sequencing analysis revealed that the ingestion of Shimanami leaf increased the abundances and diversity of intestinal bacteria, including members from Lactococcus, Streptococcus, and Muribaculaceae. Our findings suggest that Shimanami leaf supplementation improves bowel movement and promotes defecation.